Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,604 T→C 100% A410A (GCT→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6040T→C100.0% 713.5 192A410A (GCT→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (98/93):  ref base (0/1):  total (98/94)

Only 100 of 216 total aligned reads displayed.

CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531506‑3531701
                                                                                                  |                                                                                                 
cTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTa                                                                                                 >  2:178553/1‑101 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGGCGAGGGCACGATTACCGAAGACGCCTAt                                                                                                >  1:466119/1‑101 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAt                                                                                                >  2:860600/1‑101 (MQ=255)
 tGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTAt                                                                                                >  2:229667/1‑101 (MQ=255)
  gACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATc                                                                                               >  1:2968747/1‑101 (MQ=255)
   aCCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATcc                                                                                              <  2:2008478/101‑1 (MQ=255)
    ccTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCg                                                                                             <  2:1029700/101‑1 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            >  1:4188381/1‑101 (MQ=255)
     cTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGa                                                                                            >  1:1960081/1‑101 (MQ=255)
       acaacaACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAAc                                                                                          <  1:4352540/101‑1 (MQ=255)
         aacaacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACgg                                                                                        <  1:882047/101‑1 (MQ=255)
           caacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGc                                                                                      >  1:2676651/1‑101 (MQ=255)
           caacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGc                                                                                      <  1:1405035/101‑1 (MQ=255)
            aacTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCCCGATCACAGAAGACGCCTATCCGAACGGGcc                                                                                     >  1:1000919/1‑101 (MQ=255)
             acTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGccc                                                                                    >  1:4264260/1‑101 (MQ=255)
               ccGCCCAGCTTCAACTGGACGCGGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGa                                                                                  <  1:4650534/100‑1 (MQ=255)
                cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAg                                                                                 >  2:362501/1‑101 (MQ=255)
                cGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAg                                                                                 >  1:4494770/1‑101 (MQ=255)
                 gCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGc                                                                                <  1:20980/101‑1 (MQ=255)
                 gCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGc                                                                                >  1:2174609/1‑101 (MQ=255)
                   ccAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtg                                                                              >  1:988154/1‑101 (MQ=255)
                     aGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgat                                                                            <  2:2625557/101‑1 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatg                                                                           <  1:392242/101‑1 (MQ=255)
                      gCTTCAACTGGACGCTGAACCTGCGCAAGCAGGGGCGCGCGCAGTGGCTGGCCGAGGGGACGGTCACCGAAGACGACTATCCGAACCGGCCCGAGCtgatg                                                                           >  2:3910171/1‑101 (MQ=255)
                       cTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCCCGATCACCGAAGAGGCCTATCCGAACGGGCCCGAGCtgatga                                                                          >  2:1388406/1‑101 (MQ=255)
                       cTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCtgatga                                                                          >  1:2850755/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                         >  1:3618445/1‑101 (MQ=255)
                        ttCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGaa                                                                         >  2:2017614/1‑101 (MQ=255)
                         tCAACTGGACGCTGAACCGGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                        <  2:384182/101‑1 (MQ=255)
                         tCAACTGGACGCTGAACATGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAg                                                                        <  1:3332129/101‑1 (MQ=255)
                             cTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGcccc                                                                    >  2:4705452/1‑100 (MQ=255)
                               ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGGGCGGAGGAAGCCGGAc                                                                  >  2:1155109/1‑101 (MQ=255)
                               ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAc                                                                  >  1:2561464/1‑101 (MQ=255)
                               ggACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGAc                                                                  >  2:2329712/1‑101 (MQ=255)
                                gACGCTGACCCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCAGACt                                                                 <  2:1346514/101‑1 (MQ=255)
                                gACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACt                                                                 <  1:1455406/101‑1 (MQ=255)
                                   gCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATg                                                              <  2:4160640/101‑1 (MQ=255)
                                   gCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATg                                                              >  2:521890/1‑101 (MQ=255)
                                    cTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATTCGAACGGGCCCGGGCTGATGAAGCCTGACTAAga                                                             >  2:4556348/1‑101 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGGGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            <  2:2546197/101‑1 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            <  1:2803422/101‑1 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            <  2:2850579/101‑1 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            >  1:3801587/1‑101 (MQ=255)
                                     tGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgac                                                            <  2:2089961/101‑1 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           <  2:1107628/101‑1 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  1:857320/1‑101 (MQ=255)
                                      gAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATgacg                                                           >  1:1299036/1‑101 (MQ=255)
                                           cgagcAAGCAGTGACGGGCGCAGTGGCTGGCCGCGGTCGCTGCCAACAAAGCCGCCTCCCCGACCGGGCCCGAGCTGATGAAGCCCGACTACGACGACACg                                                      <  2:296424/98‑1 (MQ=255)
                                             cgcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcc                                                    >  2:4635341/1‑101 (MQ=255)
                                              gcAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGccc                                                   >  1:63770/1‑101 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGGGCTGATGAAGCCCGACTATGACGACACGcccc                                                  >  2:3120562/1‑100 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  >  2:1258328/1‑100 (MQ=255)
                                               cAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccc                                                  >  2:4327000/1‑100 (MQ=255)
                                                aaGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGcccct                                                 <  1:1496969/101‑3 (MQ=255)
                                                 aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                <  2:2174485/101‑1 (MQ=255)
                                                 aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                <  2:1901704/101‑1 (MQ=255)
                                                 aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                <  1:549653/101‑1 (MQ=255)
                                                  gCAGGGGCGCGCGCAGTGGCTGGCCGAGGGCACCAGCCCCGAAGACGCCTATCCGAAGGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                               <  1:4058906/101‑1 (MQ=255)
                                                    aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                             >  2:1228897/1‑101 (MQ=255)
                                                     ggTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGcc                                            <  2:488331/101‑1 (MQ=255)
                                                       tGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgc                                          >  1:4316924/1‑101 (MQ=255)
                                                        gcgcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCcgcg                                         >  2:2713320/1‑101 (MQ=255)
                                                         cgcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGa                                        >  2:3537884/1‑101 (MQ=255)
                                                           cgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAg                                      <  1:362535/101‑1 (MQ=255)
                                                              gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                   >  2:964226/1‑101 (MQ=255)
                                                              gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                   >  2:1563820/1‑101 (MQ=255)
                                                               cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                  >  1:1336805/1‑101 (MQ=255)
                                                                gggggctggcCGAGGGCACGATCACCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  2:1842336/98‑1 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  1:2725535/101‑1 (MQ=255)
                                                                aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                 <  2:2732566/101‑1 (MQ=255)
                                                                 gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                >  2:3778908/1‑101 (MQ=255)
                                                                  tggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGc                               >  2:1732645/1‑101 (MQ=255)
                                                                   ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACTCCCCTTTGCCGCCAAGCCGATGcc                              >  2:2817470/1‑100 (MQ=255)
                                                                     ctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCACCTCGGCCGCGAAGCGGATGCCTg                            >  2:3181662/1‑101 (MQ=255)
                                                                       ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                          >  1:1863572/1‑101 (MQ=255)
                                                                        gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                         >  2:3936732/1‑101 (MQ=255)
                                                                        gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                         <  2:1277330/101‑1 (MQ=255)
                                                                           gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                      >  1:2253548/1‑101 (MQ=255)
                                                                            ggggCCCGATCGCCGAAGACGCCGAGCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     <  1:2823726/100‑1 (MQ=255)
                                                                            aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                     >  2:3336061/1‑101 (MQ=255)
                                                                             gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                    >  1:2883256/1‑101 (MQ=255)
                                                                              ggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGc                   >  1:3097541/1‑101 (MQ=255)
                                                                               gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGcc                  <  2:2093595/101‑1 (MQ=255)
                                                                                 aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                >  1:1108230/1‑101 (MQ=255)
                                                                                 aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                <  1:959787/101‑1 (MQ=255)
                                                                                   gATCCCCGAAGCCGCCAGTCCGAACGGGCCCGAGCGGATGAAGCCCGACTATGACGACACGCCCCCCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc              <  2:4476817/101‑1 (MQ=255)
                                                                                   gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc              <  1:2954779/101‑1 (MQ=255)
                                                                                    aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa             >  2:3312969/1‑101 (MQ=255)
                                                                                       aCCGAAGACGCCTATCCGAACGGGCCGGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc          <  2:3284959/101‑1 (MQ=255)
                                                                                       aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc          >  1:802940/1‑101 (MQ=255)
                                                                                        ccGAAGACGCCTATCCGAACGGGCCCGAGCTTATGAAGCCGGGCTATGACGACACCCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc         <  1:3910413/101‑1 (MQ=255)
                                                                                        ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc         >  2:3207744/1‑101 (MQ=255)
                                                                                         cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg        >  1:4400658/1‑101 (MQ=255)
                                                                                         cGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCg        <  2:63763/101‑1 (MQ=255)
                                                                                             gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt    <  1:372423/101‑1 (MQ=255)
                                                                                             gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt    <  1:4550667/101‑1 (MQ=255)
                                                                                             gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt    <  1:1228987/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   <  1:178578/101‑1 (MQ=255)
                                                                                              aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc   >  2:905439/1‑101 (MQ=255)
                                                                                               cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg  >  1:3451587/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTGACCTACAACAACTCGCCCAGCTTCAACTGGACGCTGAACCTGCGCAAGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCG  >  NC_014034/3531506‑3531701

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 24 ≤  ATCG  < 32 ≤  ATCG  < 40 ≤  ATCG