Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,653 T→C 100% F427L (TTC→CTC)  aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6530T→C100.0% 731.2 196F427L (TTC→CTC) aceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (101/94):  ref base (0/0):  total (102/94)

Only 100 of 209 total aligned reads displayed.

AGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCC  >  NC_014034/3531555‑3531749
                                                                                                  |                                                                                                
aGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                                                                <  2:1901704/101‑1 (MQ=255)
aGCAGGTGCGCGCAAAGTGGCTGGCCGAGGGCACGACCACCGAAGACGCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTc                                                                                                <  1:4567548/101‑1 (MQ=255)
 gCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCg                                                                                               <  2:3618226/101‑1 (MQ=255)
   aGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGc                                                                                             >  2:1228897/1‑101 (MQ=255)
    ggTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGcc                                                                                            <  2:488331/101‑1 (MQ=255)
    ggTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGcc                                                                                            >  2:1410003/1‑101 (MQ=255)
        cgcgcgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGa                                                                                        >  1:2098701/1‑101 (MQ=255)
            cgcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGcc                                                                                    <  1:2790760/101‑1 (MQ=255)
             gcAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCg                                                                                   >  1:870669/1‑101 (MQ=255)
              cAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGa                                                                                  >  1:1336805/1‑101 (MQ=255)
               gggggctggcCGAGGGCACGATCACCGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                                                                 <  2:1842336/98‑1 (MQ=255)
               aGTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGAt                                                                                 <  2:1058700/101‑1 (MQ=255)
                gTGGCTGGCCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATg                                                                                >  1:713845/1‑101 (MQ=255)
                 tggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGc                                                                               >  2:1732645/1‑101 (MQ=255)
                  ggctggcCGAGGGCCGGTGCGCGGAAGACGCCTAACCGAACGGGCCTGAGCTGATGAAGCCCGACTATGACGGCACGCCCCTCGGCCGCGAAGCCGATGcc                                                                              <  2:3683100/101‑2 (MQ=255)
                  ggctggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACTCCCCTTTGCCGCCAAGCCGATGcc                                                                              >  2:2817470/1‑100 (MQ=255)
                   gctggcCGAGGGCACGATCACCGAAGACGCCTACCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGcct                                                                             <  1:4103422/101‑3 (MQ=255)
                      ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                          >  1:1985925/1‑101 (MQ=255)
                      ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                          >  1:1863572/1‑101 (MQ=255)
                       gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCt                                                                         <  2:1277330/101‑1 (MQ=255)
                        ccGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCTGCGAAGCCGATGCCTGGCTc                                                                        <  1:1840728/101‑1 (MQ=255)
                          gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                      >  1:2253548/1‑101 (MQ=255)
                           aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                     >  2:3336061/1‑101 (MQ=255)
                           aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCATCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                     >  1:4398117/1‑101 (MQ=255)
                              gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCACGGcc                                                                  >  2:2697093/1‑101 (MQ=255)
                                aCGATCCCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                <  1:446214/101‑1 (MQ=255)
                                aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                <  1:959787/101‑1 (MQ=255)
                                aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                >  1:1108230/1‑101 (MQ=255)
                                  gaccACCCAAGCCCCCTAACCGAAGGCGCCCGGGATGATCAAGCCGGACTAGGACGACCCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                              <  1:3989462/98‑1 (MQ=255)
                                  gATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                              >  2:1681775/1‑101 (MQ=255)
                                   aTCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                             <  2:4398149/101‑1 (MQ=255)
                                   aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                             >  2:3312969/1‑101 (MQ=255)
                                      aCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAc                                                          >  1:802940/1‑101 (MQ=255)
                                       ccGAAGACGCCTATCCGAACGGGCCCGAGCTTATGAAGCCGGGCTATGACGACACCCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                         <  1:3910413/101‑1 (MQ=255)
                                       ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                         >  2:2725834/1‑101 (MQ=255)
                                       ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                         >  2:3207744/1‑101 (MQ=255)
                                         gAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                       <  2:2048691/101‑1 (MQ=255)
                                          aaGTCGCCTATCCGAACGGTCCCGAGCGGATGAAGCCCGACTATGACGACACGCCCCTCCGCCCCGACGCGGATGCCTGGCTGAAGGCCTTCCGGACCggg                                                      >  2:2405392/1‑99 (MQ=255)
                                             aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                   >  2:905439/1‑101 (MQ=255)
                                             aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                   <  1:178578/101‑1 (MQ=255)
                                              cGCCTATCCGGCCGGGCCGGAGCTGTTGAAGCCCGATTATGACCACACGCCCCTCGGCCGCGAATCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                  <  2:3937368/101‑1 (MQ=255)
                                              cGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                  >  1:3451587/1‑101 (MQ=255)
                                               gcctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATagc                                                 >  2:4177558/4‑101 (MQ=255)
                                                cctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                <  1:2920394/99‑1 (MQ=255)
                                                cctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                <  2:2968559/99‑1 (MQ=255)
                                                cctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgcg                                                >  1:684644/3‑101 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                              <  1:2172247/101‑1 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                              >  1:2331638/1‑101 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                              >  2:2504078/1‑101 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                              >  1:1466891/1‑101 (MQ=255)
                                                  tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                              <  1:3718064/101‑1 (MQ=255)
                                                  tATCCAACCGGCCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                              <  2:954988/101‑1 (MQ=255)
                                                       gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                         >  2:3542825/1‑101 (MQ=255)
                                                        aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                        >  2:1810170/1‑101 (MQ=255)
                                                         aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                       <  1:3136618/101‑1 (MQ=255)
                                                         aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                       >  1:2503534/1‑101 (MQ=255)
                                                          cGGGCCCGTGCTGATGACGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcgg                                      <  2:4264013/101‑1 (MQ=255)
                                                           gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                     >  1:54049/1‑101 (MQ=255)
                                                           gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                     >  2:2096685/1‑101 (MQ=255)
                                                            ggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCATACCGAGATCGCGACGCGGGCgggg                                    >  1:2612976/1‑101 (MQ=255)
                                                            ggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                    <  1:3435177/101‑1 (MQ=255)
                                                            ggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                    >  1:3347299/1‑101 (MQ=255)
                                                            ggCCCGAGCTGACGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                    <  1:2009700/101‑1 (MQ=255)
                                                              cccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                  <  1:4327247/101‑1 (MQ=255)
                                                               ccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtg                                 >  1:1578211/1‑101 (MQ=255)
                                                                cGAGCTAATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                <  1:755674/101‑1 (MQ=255)
                                                                 gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGGGATCGCGACGCGGGCGGGGgtggt                               >  1:397771/1‑99 (MQ=255)
                                                                 gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGtt                               <  2:4494813/101‑1 (MQ=255)
                                                                   gCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTcc                             <  1:568374/101‑1 (MQ=255)
                                                                    cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                            >  1:2970203/1‑101 (MQ=255)
                                                                     tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                           >  1:1922656/1‑101 (MQ=255)
                                                                     tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                           >  1:3031284/1‑101 (MQ=255)
                                                                      gatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcaca                          >  1:3253070/1‑101 (MQ=255)
                                                                        tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                        <  2:2210479/101‑1 (MQ=255)
                                                                         gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAcc                       <  2:3097341/101‑1 (MQ=255)
                                                                          aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                      <  2:2883080/101‑1 (MQ=255)
                                                                          aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCt                      >  1:648307/1‑101 (MQ=255)
                                                                          aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCt                      >  2:3375098/1‑101 (MQ=255)
                                                                           aGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                     <  1:1732750/101‑1 (MQ=255)
                                                                           aGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                     >  1:4134449/1‑101 (MQ=255)
                                                                            gCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGa                    <  1:3313171/101‑1 (MQ=255)
                                                                              ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                  >  2:576323/1‑101 (MQ=255)
                                                                              ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                  <  2:1959964/101‑1 (MQ=255)
                                                                                   tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt             <  1:2864530/101‑1 (MQ=255)
                                                                                    aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg            >  1:1253340/1‑101 (MQ=255)
                                                                                    aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTg            <  2:2331505/101‑1 (MQ=255)
                                                                                     tGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGc           >  1:2312106/1‑101 (MQ=255)
                                                                                      gacgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGcc          >  1:2824227/1‑101 (MQ=255)
                                                                                      gacgacACGCCCCGCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTCCCACAACCTGATCACCCTGcc          <  1:2697247/101‑1 (MQ=255)
                                                                                       acgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc         <  2:3801352/101‑1 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa        <  2:1482100/101‑1 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa        <  2:217569/101‑1 (MQ=255)
                                                                                        cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGCGTTCCACAACCTGATCACCCTGCCCa        >  2:2603234/1‑101 (MQ=255)
                                                                                         gacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAc       >  1:1264175/1‑101 (MQ=255)
                                                                                         gacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAc       <  2:3451375/101‑1 (MQ=255)
                                                                                          acacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAcc      >  2:694323/1‑101 (MQ=255)
                                                                                           cacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCt     <  2:553073/101‑1 (MQ=255)
                                                                                             cGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTc   <  2:802867/101‑1 (MQ=255)
                                                                                              gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc  >  2:2128420/1‑101 (MQ=255)
                                                                                              gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc  <  1:993058/101‑1 (MQ=255)
                                                                                                  |                                                                                                
AGCAGGTGCGCGCGCAGTGGCTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCC  >  NC_014034/3531555‑3531749

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG