Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,531,673 G→C 100% A433A (GCG→GCC aceA → isocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,531,6730G→C100.0% 815.4 212A433A (GCG→GCCaceAisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (104/108):  ref base (0/0):  total (104/108)

Only 100 of 217 total aligned reads displayed.

CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770
                                                                                                  |                                                                                                 
  ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                                               >  1:1863572/1‑101 (MQ=255)
  ggcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGc                                                                                               >  1:1985925/1‑101 (MQ=255)
   gcCGAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCTGCCGCGAAGCCGATGCCTGGCt                                                                                              <  2:1976295/101‑1 (MQ=255)
      gAGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCaa                                                                                           >  1:2253548/1‑101 (MQ=255)
       aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                                          >  2:3336061/1‑101 (MQ=255)
       aGGGCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCATCGGCCGCGAAGCCGATGCCTGGCTCAAg                                                                                          >  1:4398117/1‑101 (MQ=255)
        gggCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAgg                                                                                         >  1:2883256/1‑101 (MQ=255)
          gCACGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCACGGcc                                                                                       >  2:2697093/1‑101 (MQ=255)
            aCGATCCCCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                                     <  1:446214/101‑1 (MQ=255)
            aCGATCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCtt                                                                                     <  1:1258418/101‑1 (MQ=255)
              gatccgcGAAGCCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGACCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  2:846060/95‑1 (MQ=255)
              gaccACCCAAGCCCCCTAACCGAAGGCGCCCGGGATGATCAAGCCGGACTAGGACGACCCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  1:3989462/98‑1 (MQ=255)
              gATCCCCGAAGCCGCCAGTCCGAACGGGCCCGAGCGGATGAAGCCCGACTATGACGACACGCCCCCCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTcc                                                                                   <  2:4476817/101‑1 (MQ=255)
               aTCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                                                  <  2:4398149/101‑1 (MQ=255)
               aTCACCGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCa                                                                                  >  2:3312969/1‑101 (MQ=255)
                   ccGAAGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGAcc                                                                              >  2:3207744/1‑101 (MQ=255)
                     gAAGCCGCCTATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCga                                                                            <  2:2048691/101‑1 (MQ=255)
                      aaGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgag                                                                           >  1:2851081/1‑101 (MQ=255)
                       aGACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCgaga                                                                          >  2:1035886/1‑101 (MQ=255)
                        gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                                         <  1:372423/101‑1 (MQ=255)
                        gACGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGAt                                                                         <  1:1228987/101‑1 (MQ=255)
                         aCGCCTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATc                                                                        >  2:1231331/1‑101 (MQ=255)
                          cGCCTATCCGGCCGGGCCGGAGCTGTTGAAGCCCGATTATGACCACACGCCCCTCGGCCGCGAATCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcg                                                                       <  2:3937368/101‑1 (MQ=255)
                           gcctATCCGACCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATcgc                                                                      <  2:1374709/98‑1 (MQ=255)
                           gcctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATagc                                                                      >  2:4177558/4‑101 (MQ=255)
                             ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                                    >  1:1785886/2‑101 (MQ=255)
                             ctATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGa                                                                    >  2:2270838/2‑101 (MQ=255)
                              tATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                                                   <  1:2172247/101‑1 (MQ=255)
                              tATCCAACCGGCCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAc                                                                   <  2:954988/101‑1 (MQ=255)
                                tCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGAcgc                                                                 >  1:1735757/1‑101 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  2:3542825/1‑101 (MQ=255)
                                   gAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcggg                                                              >  1:1461201/1‑101 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                                             >  2:1810170/1‑101 (MQ=255)
                                    aaCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggc                                                             >  2:2399828/1‑101 (MQ=255)
                                     aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                                            <  1:3136618/101‑1 (MQ=255)
                                     aCGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcg                                                            >  1:2503534/1‑101 (MQ=255)
                                      cGGGCCCGTGCTGATGACGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACgcgggcgg                                                           <  2:4264013/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  2:2096685/1‑101 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  1:54049/1‑101 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          <  1:3291561/101‑1 (MQ=255)
                                       gggCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGcgggcggg                                                          >  1:3448155/1‑101 (MQ=255)
                                        ggCCCGAGCTGACGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                                         <  1:2009700/101‑1 (MQ=255)
                                        ggCCAGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCgggg                                                         <  1:1588781/101‑1 (MQ=255)
                                          cccGAGCTGATGAAGCCCGACTATGCCGACGCGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       <  1:1877841/101‑1 (MQ=255)
                                          cccGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgt                                                       <  1:4327247/101‑1 (MQ=255)
                                            cGAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                                     <  1:679688/101‑1 (MQ=255)
                                            cGAGCTAATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGgtgt                                                     <  1:755674/101‑1 (MQ=255)
                                             gAGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGGGATCGCGACGCGGGCGGGGgtggt                                                    >  1:397771/1‑99 (MQ=255)
                                              aGCTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTc                                                   >  2:3075737/1‑101 (MQ=255)
                                                cTGATGAAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 >  1:2970203/1‑101 (MQ=255)
                                                cTGATGAACCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCca                                                 <  2:3637460/101‑1 (MQ=255)
                                                 tgatgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCcac                                                >  1:3031284/1‑101 (MQ=255)
                                                    tgaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAc                                             <  2:2210479/101‑1 (MQ=255)
                                                     gaAGCCCGACTATGCCGACACACCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAcc                                            <  1:1563921/101‑1 (MQ=255)
                                                     gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGCGATCGGGGCGCGGGCGGTGGGGTTCCACAAcc                                            >  2:2942970/1‑101 (MQ=255)
                                                     gaAGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAAcc                                            <  2:3097341/101‑1 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTGGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           >  2:526210/1‑101 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           <  2:2883080/101‑1 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCt                                           >  1:699215/1‑101 (MQ=255)
                                                      aaGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCt                                           >  2:3375098/1‑101 (MQ=255)
                                                       aGCCCGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTg                                          >  1:4134449/1‑101 (MQ=255)
                                                         cccGACTAGGACGACACGCCCCGCGGCCCGGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGCCGCGGGCGGGGGTGTTCCACAACCTGAt                                        <  2:1915336/101‑1 (MQ=255)
                                                          ccGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATc                                       >  2:576323/1‑101 (MQ=255)
                                                           cGACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCa                                      >  1:641669/1‑101 (MQ=255)
                                                            gACTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAc                                     >  2:1708566/1‑101 (MQ=255)
                                                              cTATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCAccc                                   >  2:2837137/1‑101 (MQ=255)
                                                               tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt                                  <  2:713772/101‑1 (MQ=255)
                                                               tATGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCt                                  <  1:2329845/101‑1 (MQ=255)
                                                                aTGACGACACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTg                                 >  1:869092/1‑101 (MQ=255)
                                                                  gacgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGcc                               >  1:2824227/1‑101 (MQ=255)
                                                                   acgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGccc                              <  2:3801352/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             <  2:1482100/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCa                             <  2:217569/101‑1 (MQ=255)
                                                                    cgacACGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGCGTTCCACAACCTGATCACCCTGCCCa                             >  2:2603234/1‑101 (MQ=255)
                                                                      ccacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCAcc                           <  1:3347243/100‑1 (MQ=255)
                                                                       cacGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGGGTTCCACAACCTGATCACCCTGCCCACCt                          >  1:950568/1‑101 (MQ=255)
                                                                        acGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCtt                         <  2:2503390/101‑1 (MQ=255)
                                                                         cGCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTc                        >  1:2676005/1‑101 (MQ=255)
                                                                          gCCCCTCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTcc                       >  2:2128420/1‑101 (MQ=255)
                                                                            ccctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCAt                     <  1:2270971/98‑1 (MQ=255)
                                                                            ccctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCAt                     <  2:1319976/98‑1 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGGGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   >  1:4640064/2‑101 (MQ=255)
                                                                              ctCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCt                   <  1:3538103/100‑1 (MQ=255)
                                                                               tCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTg                  <  2:1863460/101‑1 (MQ=255)
                                                                               tCGGCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTg                  <  1:2751493/101‑1 (MQ=255)
                                                                                 ggCCGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGAc                >  1:356063/1‑101 (MQ=255)
                                                                                   ccGCGAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACcg              >  2:2773818/1‑101 (MQ=255)
                                                                                      cgAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGa           >  1:3858970/1‑101 (MQ=255)
                                                                                       gAAGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaa          <  1:2128541/101‑1 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         >  2:4084733/1‑101 (MQ=255)
                                                                                        aaGCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGaaa         <  2:684580/101‑1 (MQ=255)
                                                                                          gCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATc       >  1:320296/1‑101 (MQ=255)
                                                                                          gCCGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATc       >  2:4578760/1‑101 (MQ=255)
                                                                                           ccGATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCg      >  2:1410410/1‑101 (MQ=255)
                                                                                             gATGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTATTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgat    <  2:2004456/101‑1 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACGTTCCATCTGACCGCGAAATCgatg   >  1:1851695/1‑101 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   >  1:3378329/1‑101 (MQ=255)
                                                                                              aTGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatg   <  1:1037801/101‑1 (MQ=255)
                                                                                               tGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  >  2:3896651/1‑101 (MQ=255)
                                                                                               tGCCTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCgatgg  <  1:964303/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CTGGCCGAGGGCACGATCACCGAAGACGCTTATCCGAACGGGCCCGAGCTGATGAAGCCCGACTATGACGACACGCCCTTCGGCCGCGAAGCCGATGCGTGGCTCAAGGCCTTCCAGACCGAGATCGCGACGCGGGCGGGGGTGTTCCACAACCTGATCACCCTGCCCACCTTCCATCTGACCGCGAAATCGATGG  >  NC_014034/3531575‑3531770

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG