Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,539,498 T→G 100% D261E (GAT→GAG prpD → 2‑methylcitrate dehydratase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,539,4980T→G100.0% 891.3 239D261E (GAT→GAGprpD2‑methylcitrate dehydratase
Reads supporting (aligned to +/- strand):  new base (120/119):  ref base (0/0):  total (120/119)

Only 100 of 264 total aligned reads displayed.

ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595
                                                                                                  |                                                                                                 
   gAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGcgcg                                                                                              <  2:1037651/101‑1 (MQ=255)
    aGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGt                                                                                             <  2:789154/101‑1 (MQ=255)
    aGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGt                                                                                             >  1:987391/1‑101 (MQ=255)
     ggCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTAGGGCCCAGAGGCGAGtt                                                                                            >  2:1478014/1‑101 (MQ=255)
       cGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTac                                                                                          >  1:3449267/1‑101 (MQ=255)
        ggTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTaca                                                                                         <  1:3892357/101‑1 (MQ=255)
        ggTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTaca                                                                                         >  1:3064303/1‑101 (MQ=255)
        ggTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTaca                                                                                         <  2:1152815/101‑1 (MQ=255)
        ggTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTaca                                                                                         <  1:1961515/101‑1 (MQ=255)
         gTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTacac                                                                                        >  1:4293722/1‑101 (MQ=255)
          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       >  2:2008353/1‑101 (MQ=255)
          tGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACAcc                                                                                       >  1:3871991/1‑101 (MQ=255)
             aCCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTc                                                                                    <  1:4029157/101‑1 (MQ=255)
             aCCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTc                                                                                    >  2:1755446/1‑101 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:379422/101‑1 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   <  1:2801972/101‑1 (MQ=255)
              ccGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTcc                                                                                   >  2:42352/1‑101 (MQ=255)
               cgcgCCGTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCg                                                                                  <  1:777487/101‑1 (MQ=255)
               cgcgCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCg                                                                                  <  1:1670624/101‑1 (MQ=255)
                gcgcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGc                                                                                 >  2:1267369/1‑101 (MQ=255)
                 cgcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGcc                                                                                <  2:3885550/101‑1 (MQ=255)
                 cgcCCGGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGcc                                                                                <  2:943234/101‑1 (MQ=255)
                  gcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCt                                                                               >  1:1351590/1‑101 (MQ=255)
                  gcCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCt                                                                               >  2:2279957/1‑101 (MQ=255)
                   cccTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTg                                                                              <  2:367669/101‑1 (MQ=255)
                        cgcgGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCgg                                                                         >  2:4609795/1‑101 (MQ=255)
                           ggcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCa                                                                      >  2:112880/1‑101 (MQ=255)
                            gcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCAt                                                                     <  1:1586447/101‑1 (MQ=255)
                            gcgAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCAt                                                                     >  1:1753857/1‑101 (MQ=255)
                              gAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   >  1:2185779/1‑101 (MQ=255)
                              gAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATgg                                                                   >  2:3443881/1‑101 (MQ=255)
                               aaGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGc                                                                  >  2:962351/1‑101 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 <  2:1383897/101‑1 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 >  2:2710426/1‑101 (MQ=255)
                                aGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCg                                                                 >  2:296077/1‑101 (MQ=255)
                                 ggCGCCCCCAGCCCCGCCTGGGAGGGGGAAGAAGGTTTCATTGCCTGGATGCTCTCGGGCCCAGAGACGCGTTACACCGGCCGCCCTCCCGGccctagaga                                                                >  2:3144163/1‑94 (MQ=255)
                                   cgcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGc                                                              <  1:920406/101‑1 (MQ=255)
                                    gcCCCCAGCCCCGCCTGGGAGGGGGAAGACGGGTTCATAGCCTGGATGCTCTCGGGACCAGAGGAGCGTTAAACCCACACCCTCCCCGACAATGGCGAGca                                                             >  2:611126/1‑100 (MQ=255)
                                      ccccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGa                                                           <  1:1253796/101‑1 (MQ=255)
                                       cccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaa                                                          <  1:2469873/101‑1 (MQ=255)
                                        ccAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGaaa                                                         <  1:1147897/101‑1 (MQ=255)
                                         cAGCCCCGCCTGGGAGGTGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTCCACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAc                                                        <  2:528868/101‑1 (MQ=255)
                                          aGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       >  1:1780565/1‑101 (MQ=255)
                                          aGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcg                                                       <  2:1556929/101‑1 (MQ=255)
                                           gCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAAcgc                                                      <  1:3187259/101‑1 (MQ=255)
                                               cGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCa                                                  >  2:451374/1‑101 (MQ=255)
                                                  cTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATcc                                               >  2:1311039/1‑101 (MQ=255)
                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             >  2:4396276/1‑101 (MQ=255)
                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  1:2995332/101‑1 (MQ=255)
                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  1:3554357/101‑1 (MQ=255)
                                                    gggAGGGGGAAGACGGTTTCATCGCCTGGAGGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCtt                                             <  1:940781/101‑1 (MQ=255)
                                                      gAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGa                                           >  2:2543725/1‑101 (MQ=255)
                                                       aGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGac                                          >  2:2150555/1‑101 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         >  2:647353/1‑101 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGaca                                         <  1:3279724/101‑1 (MQ=255)
                                                        gggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCCAGCCGAAACGCGCCATCCTTGaca                                         >  2:1822951/1‑101 (MQ=255)
                                                         ggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGacac                                        <  2:3515266/101‑1 (MQ=255)
                                                         ggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGacac                                        >  2:3267670/1‑101 (MQ=255)
                                                         ggggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGacac                                        >  2:4150642/1‑101 (MQ=255)
                                                          gggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACAcc                                       <  2:745472/101‑1 (MQ=255)
                                                           ggAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCt                                      >  2:2817390/1‑101 (MQ=255)
                                                            gAAGACGTTTTCACCGCCTGGATGCTCCCGGGCCCAGAGGCGCGTTACACCGTCCCCCTTCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     <  1:3687228/101‑1 (MQ=255)
                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     <  1:1713635/101‑1 (MQ=255)
                                                            gAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCta                                     >  2:3737513/1‑101 (MQ=255)
                                                             aaGCCGTTTTCACCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  2:489920/101‑1 (MQ=255)
                                                             aaGACGGTTTCCCCGCCGGGATGCTCTCGGACCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  1:140563/101‑1 (MQ=255)
                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    >  2:2687361/1‑101 (MQ=255)
                                                             aaGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    <  1:2940135/101‑1 (MQ=255)
                                                             aaGACGGTTTCAGCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtat                                    >  1:2577156/1‑101 (MQ=255)
                                                              aGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCtata                                   >  1:1471489/1‑101 (MQ=255)
                                                                gcGGTTTCATCGCCTGGATGCTCTGGGGCCCGGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGGGAGCCGAAACGCGCCATCCTTGACACCTATAcc                                 <  1:1309329/100‑1 (MQ=255)
                                                                 cGGTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCa                                <  1:2742059/101‑1 (MQ=255)
                                                                  ggTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCaa                               <  1:2008470/101‑1 (MQ=255)
                                                                  ggTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCaa                               >  1:3443336/1‑101 (MQ=255)
                                                                  ggTTTCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCaa                               >  2:2494938/1‑101 (MQ=255)
                                                                     ttCATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGACACGCGCCATCCTTGACACCTATACCAAGGa                            >  2:1393553/1‑101 (MQ=255)
                                                                      tCANCNNCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGaa                           <  1:2366105/101‑1 (MQ=255)
                                                                       cATCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAat                          >  1:474015/1‑101 (MQ=255)
                                                                        aTCGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAata                         >  2:265610/1‑101 (MQ=255)
                                                                         tggCCTGGATGCTCACGGGCCCGGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  2:1465830/99‑1 (MQ=255)
                                                                         tCGCCTGGATCCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAatat                        <  1:2114755/101‑1 (MQ=255)
                                                                          cGCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATAtt                       <  1:3629063/101‑1 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      <  1:451418/101‑1 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      >  2:4203994/1‑101 (MQ=255)
                                                                           gCCTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTc                      <  2:4293475/101‑1 (MQ=255)
                                                                            ccTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcg                     >  2:2401506/1‑101 (MQ=255)
                                                                             cTGGATGCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTcgg                    >  2:191261/1‑101 (MQ=255)
                                                                                   gCTCTCGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAat              <  1:1042386/101‑1 (MQ=255)
                                                                                   gCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCGATGGCGAGCCGAAACGCGCTATGCTTGACACCTATACCAAGGAATATTCGGCGGAat              >  1:4056817/1‑101 (MQ=255)
                                                                                   gCTCTCGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAat              >  1:2328485/1‑101 (MQ=255)
                                                                                     tctcGGGCCCAGAGGCGCGTTACCCCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAatat            <  1:2883172/101‑1 (MQ=255)
                                                                                        cGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         <  1:2280090/101‑1 (MQ=255)
                                                                                        cGGGCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaa         >  1:1563319/1‑101 (MQ=255)
                                                                                         gggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        >  1:578914/1‑101 (MQ=255)
                                                                                         gggCCCAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        >  1:4177888/1‑101 (MQ=255)
                                                                                         gggCCAGGAGGCGCGTTCCACCGTCCGCCCGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCaaa        <  2:1613721/101‑1 (MQ=255)
                                                                                            cccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGcc     <  1:66069/101‑1 (MQ=255)
                                                                                             ccAGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCa    >  2:2898444/1‑101 (MQ=255)
                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  >  2:3358534/1‑101 (MQ=255)
                                                                                               aGAGGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAgg  >  2:1054891/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
ATCGAGGCGGTGGACCGCGCCCTGCGCGGCGAAGGCGCCCCCAGCCCCGCCTGGGAGGGGGAAGACGGTTTCATCGCCTGGATGCTCTCGGGCCCAGATGCGCGTTACACCGTCCGCCTGCCCGGCCATGGCGAGCCGAAACGCGCCATCCTTGACACCTATACCAAGGAATATTCGGCGGAATATCAAAGCCAGG  >  NC_014034/3539400‑3539595

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG