Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,541,575 G→C 100% S53S (TCG→TCC prpB → methylisocitrate lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,541,5750G→C100.0% 845.1 221S53S (TCG→TCCprpBmethylisocitrate lyase
Reads supporting (aligned to +/- strand):  new base (111/110):  ref base (0/0):  total (111/110)

Only 100 of 236 total aligned reads displayed.

GCCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541477‑3541672
                                                                                                  |                                                                                                 
 cccTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGgc                                                                                                <  2:2667568/101‑1 (MQ=255)
   cTGGCCCGGCGCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGACGCGGGGGTTCAGGCGCTGTATCTATCCGgcgc                                                                                              >  1:4098534/1‑101 (MQ=255)
    tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             <  1:1051596/101‑1 (MQ=255)
    tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             <  2:702624/101‑1 (MQ=255)
    tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             <  1:2440372/101‑1 (MQ=255)
    tGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGcgcg                                                                                             >  1:2829010/1‑101 (MQ=255)
     ggcccggaccGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTCTATCTGTCCGGCgcgg                                                                                            >  2:3047017/1‑101 (MQ=255)
       cccggcccggCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcggcg                                                                                          <  1:848069/101‑1 (MQ=255)
       cccggcccggCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCgcggcg                                                                                          <  1:1227602/101‑1 (MQ=255)
         cggcccggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGAt                                                                                        >  1:1457700/1‑101 (MQ=255)
              cggcATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCgg                                                                                   >  1:1635034/1‑101 (MQ=255)
                 cATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCt                                                                                >  1:4484115/1‑101 (MQ=255)
                    ccTGAGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCACGGGGATTCAGGGGCTTTATCTGTCCGGCGCGGAGATGTCGGCCTCGa                                                                             >  2:490575/1‑101 (MQ=255)
                      tGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATg                                                                           >  1:721721/1‑101 (MQ=255)
                          ccTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGcc                                                                       >  1:1748378/1‑101 (MQ=255)
                          ccTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGcc                                                                       <  2:3235921/101‑1 (MQ=255)
                           cTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCt                                                                      >  2:2952523/1‑101 (MQ=255)
                           cTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCt                                                                      <  2:1570002/101‑1 (MQ=255)
                           cTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCt                                                                      <  1:3162095/101‑1 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     >  1:3898715/1‑101 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     <  1:3277425/101‑1 (MQ=255)
                            tGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTg                                                                     >  2:2509187/1‑101 (MQ=255)
                               cGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCg                                                                  >  1:2539363/1‑101 (MQ=255)
                                 ggCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGa                                                                >  1:2569594/1‑101 (MQ=255)
                                   cgcgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATTTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGAcc                                                              <  2:2422239/101‑1 (MQ=255)
                                     cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            <  2:4628886/101‑1 (MQ=255)
                                     cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            >  1:1358943/1‑101 (MQ=255)
                                     cgcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTc                                                            <  1:1270722/101‑1 (MQ=255)
                                      gcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCg                                                           <  1:3133105/101‑1 (MQ=255)
                                      gcATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCg                                                           >  1:3479991/1‑101 (MQ=255)
                                       cATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCgg                                                          <  2:875871/101‑1 (MQ=255)
                                       cATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCgg                                                          >  2:2617108/1‑101 (MQ=255)
                                        acaaCGGGCTGGCCCCGCGGCAGGCTAAAGCCGCGGTGTTTCAGGCGCTTTATCTGCCCGGCGCGGCGATGGCGGCCTCGATGGGCCTCCCGGACCTCGGc                                                         <  2:848979/99‑1 (MQ=255)
                                        aTAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGc                                                         >  2:126713/1‑101 (MQ=255)
                                        aTAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGc                                                         >  2:1413343/1‑101 (MQ=255)
                                         tAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCa                                                        >  2:4493011/1‑101 (MQ=255)
                                          aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCCGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       >  1:3060203/1‑101 (MQ=255)
                                          aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       >  1:4736575/1‑101 (MQ=255)
                                          aaCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCAt                                                       <  2:3838823/101‑1 (MQ=255)
                                           aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      >  1:914781/1‑101 (MQ=255)
                                           aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      >  1:1929843/1‑101 (MQ=255)
                                           aCGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATc                                                      >  1:3576338/1‑101 (MQ=255)
                                            cGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATcc                                                     <  2:3390227/101‑1 (MQ=255)
                                            cGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATcc                                                     <  2:1537514/101‑1 (MQ=255)
                                            cGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATcc                                                     <  1:3860429/101‑1 (MQ=255)
                                             ggCATGGCCGCGCTGCAGGCGAAAGCCGCTGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                    <  2:1621964/101‑1 (MQ=255)
                                             ggCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCt                                                    <  1:4014210/101‑1 (MQ=255)
                                              gCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTg                                                   <  1:3065589/101‑1 (MQ=255)
                                               cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                  <  1:4710716/101‑1 (MQ=255)
                                               cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                  >  1:493630/1‑101 (MQ=255)
                                               cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGa                                                  <  2:1457606/101‑1 (MQ=255)
                                               cATGGCCGCGCTGCAGGCGAAAGCCGCGGGGGTTCAGGGGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATTCGCCTGCCGGAACTTGGCATCCTgc                                                  >  2:3270896/1‑100 (MQ=255)
                                                aTGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGAc                                                 >  2:3926065/1‑101 (MQ=255)
                                                     cgcgCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTgg                                            >  1:1831341/1‑101 (MQ=255)
                                                      gcacTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGa                                           <  1:3386949/98‑1 (MQ=255)
                                                       cgcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGCCCTGCCGGACCTCGGCATCCTGACGGTGGAc                                          <  1:4203662/101‑1 (MQ=255)
                                                        gcTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACg                                         <  1:1977564/101‑1 (MQ=255)
                                                         cTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGa                                        <  2:4345658/101‑1 (MQ=255)
                                                          tGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAg                                       >  1:2052260/1‑101 (MQ=255)
                                                           gCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAgg                                      <  2:1183880/101‑1 (MQ=255)
                                                             cggCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTc                                    <  2:4044829/100‑1 (MQ=255)
                                                               gCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGc                                  >  2:3800572/1‑101 (MQ=255)
                                                                cGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGcc                                 >  1:1738713/1‑101 (MQ=255)
                                                                cGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGcc                                 >  1:303975/1‑101 (MQ=255)
                                                                 gAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCt                                >  2:3619713/1‑101 (MQ=255)
                                                                  aaaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtt                               >  1:1470909/1‑101 (MQ=255)
                                                                   aaGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttt                              <  2:2752494/101‑1 (MQ=255)
                                                                    aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                             <  2:3670909/101‑1 (MQ=255)
                                                                    aGCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCtttt                             >  1:2394047/1‑101 (MQ=255)
                                                                     gCCGCGGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCttttt                            <  2:2041182/101‑1 (MQ=255)
                                                                       cgcgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCa                          <  2:233883/101‑1 (MQ=255)
                                                                         cgGGGTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATc                        >  1:2365584/1‑101 (MQ=255)
                                                                          ggggTTTCAGGCGTTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATcc                       <  1:3176867/101‑1 (MQ=255)
                                                                           gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCg                      <  1:3663094/101‑1 (MQ=255)
                                                                           gggTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCg                      <  1:2039097/101‑1 (MQ=255)
                                                                            ggTTTCAGGCGCTTTATGTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGc                     <  2:4268626/101‑1 (MQ=255)
                                                                             gTTTCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGcc                    >  2:4072272/1‑101 (MQ=255)
                                                                              tttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCa                   >  1:978725/1‑101 (MQ=255)
                                                                               ttCAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAg                  <  1:381592/101‑1 (MQ=255)
                                                                                 cAGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGGCGCCTTTTTCATCCGCCAGAt                >  2:2163986/1‑101 (MQ=255)
                                                                                  aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               <  1:4493261/101‑1 (MQ=255)
                                                                                  aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               <  2:4452677/101‑1 (MQ=255)
                                                                                  aGGCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATc               >  2:2214720/1‑101 (MQ=255)
                                                                                   ggCGCTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCa              <  1:4622653/101‑1 (MQ=255)
                                                                                   ggCCCTTTATCGGTTCGGTGCGTCGATGTCGGCCGCGATGGTCCTGCCGGGCCTCGGCATCCTGACGGTGGACGAGGTCGGCTTTTTCATCCGCCAGATCa              <  2:2900356/101‑1 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGCCCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             >  2:635408/1‑101 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCag             >  1:610478/1‑100 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             <  1:2243429/101‑1 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             <  1:2950626/101‑1 (MQ=255)
                                                                                    gcgcTTTATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             <  2:1390589/101‑1 (MQ=255)
                                                                                    gcgcTTTATCTGCCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCAc             <  2:285193/101‑1 (MQ=255)
                                                                                       cTTTTTTTGTCCGGCTCCGCTATTTCCGCCCCGGTGGGCCCTCCCGGCCTTGGCCACCCGGCGGGGGGCGAAGGCGCCTTTTTCATCCGCCAGATCAcgcg          <  2:2448054/101‑1 (MQ=255)
                                                                                        tttATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCgg         <  1:3428824/101‑1 (MQ=255)
                                                                                          tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGGTCACGCGGGc       >  2:1988738/1‑101 (MQ=255)
                                                                                          tATCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGc       <  1:4332937/101‑1 (MQ=255)
                                                                                           aTCTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGcc      >  2:1211132/1‑101 (MQ=255)
                                                                                             cTGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTc    <  2:560258/101‑1 (MQ=255)
                                                                                              tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   >  1:4244793/1‑101 (MQ=255)
                                                                                              tGTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCg   >  2:3004218/1‑101 (MQ=255)
                                                                                               gTCCGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCgg  >  2:352746/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
GCCCTGGCCCGGCCCGGCATCCTGCGCCTGCCGGGCGCGCATAACGGCATGGCCGCGCTGCAGGCGAAAGCCGCGGGGTTTCAGGCGCTTTATCTGTCGGGCGCGGCGATGTCGGCCTCGATGGGCCTGCCGGACCTCGGCATCCTGACGGTGGACGAGGTCGCCTTTTTCATCCGCCAGATCACGCGGGCCTCGG  >  NC_014034/3541477‑3541672

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG