Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 362,920 C→T 100% A5V (GCA→GTA)  bztA → glutamate/aspartate ABC transporter periplasmic glutamate/aspartate‑binding protein BztA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034362,9200C→T100.0% 943.1 245A5V (GCA→GTA) bztAglutamate/aspartate ABC transporter periplasmic glutamate/aspartate‑binding protein BztA
Reads supporting (aligned to +/- strand):  new base (130/115):  ref base (0/0):  total (130/115)

Only 100 of 249 total aligned reads displayed.

AGCCGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGCATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGATCTGCGGC  >  NC_014034/362822‑363017
                                                                                                  |                                                                                                 
aGCCGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAt                                                                                                 >  2:1178672/1‑101 (MQ=255)
aGCCGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAt                                                                                                 >  1:4527584/1‑101 (MQ=255)
 gCCGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAtt                                                                                                <  2:623186/101‑1 (MQ=255)
 gCCGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAtt                                                                                                <  1:1807756/101‑1 (MQ=255)
   cGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAttct                                                                                              >  2:4553834/1‑101 (MQ=255)
    ggTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAttctt                                                                                             <  2:4482660/101‑1 (MQ=255)
    ggTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAttctt                                                                                             <  2:3710682/101‑1 (MQ=255)
     gTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTAttcttc                                                                                            <  1:1362336/101‑1 (MQ=255)
       gatgatCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCgg                                                                                          <  2:2941594/101‑1 (MQ=255)
         tgatCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCt                                                                                        <  2:1398458/101‑1 (MQ=255)
            tCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCgg                                                                                     <  1:3755907/101‑1 (MQ=255)
            tCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCgg                                                                                     <  1:385423/101‑1 (MQ=255)
            tCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACAGCCGCAACGGCGGCAACTCAGGAAGAGGGAAAAATGAAAAAATCCGTATTATTTGCCTCgg                                                                                     >  2:2020679/1‑101 (MQ=255)
              ggCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTc                                                                                   <  1:1478987/101‑1 (MQ=255)
              ggCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTc                                                                                   >  2:2306579/1‑101 (MQ=255)
                cacaGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGc                                                                                 >  1:3529429/1‑101 (MQ=255)
                cacaGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGc                                                                                 <  2:4085746/101‑1 (MQ=255)
                   aGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACt                                                                              <  1:3042993/101‑1 (MQ=255)
                    gAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTg                                                                             <  2:1785833/101‑1 (MQ=255)
                         tGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGgccgc                                                                        >  1:1888230/1‑101 (MQ=255)
                           cAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGccc                                                                      <  1:2189287/101‑1 (MQ=255)
                           cAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGccc                                                                      >  1:44719/1‑101 (MQ=255)
                            aGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGcccc                                                                     >  1:3978622/1‑100 (MQ=255)
                            aGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCt                                                                     >  1:466643/1‑101 (MQ=255)
                            aGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCt                                                                     <  1:1059418/101‑1 (MQ=255)
                             gACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTc                                                                    <  2:3837542/101‑1 (MQ=255)
                             gACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTc                                                                    <  2:482268/101‑1 (MQ=255)
                             gACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTc                                                                    <  2:1271908/101‑1 (MQ=255)
                              aCCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTcc                                                                   >  1:3817854/1‑100 (MQ=255)
                              aCCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCg                                                                   <  1:186777/101‑1 (MQ=255)
                               cccGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGt                                                                  >  2:2285219/1‑101 (MQ=255)
                                  gTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggc                                                               >  2:2576439/1‑101 (MQ=255)
                                  gTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggc                                                               <  1:2996681/101‑1 (MQ=255)
                                  gTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggc                                                               <  2:1888118/101‑1 (MQ=255)
                                   tCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggcg                                                              >  2:725103/1‑101 (MQ=255)
                                    cAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGGCGCACTGGCCGCCCTCGTggcgg                                                             >  2:1042070/1‑101 (MQ=255)
                                     aaGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggcggg                                                            <  2:3228739/101‑1 (MQ=255)
                                     aaGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggcggg                                                            >  1:3472909/1‑101 (MQ=255)
                                       gggCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGGCGCACTTGCCGCCCTCGTggcgcgcg                                                          >  2:4444659/1‑101 (MQ=255)
                                        ggCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTggcgcgcgg                                                         >  2:3029840/1‑100 (MQ=255)
                                        ggCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGGAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTTGCCGCCCTCGTggcgggggg                                                         >  1:4573328/1‑98 (MQ=255)
                                          cAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGgcgg                                                       >  2:2076164/1‑101 (MQ=255)
                                           aGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGggggc                                                      >  2:4274314/1‑101 (MQ=255)
                                           aGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCggggg                                                      >  2:2461278/1‑100 (MQ=255)
                                           aGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCgcggc                                                      <  2:3117725/101‑1 (MQ=255)
                                             aCTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGggggcgg                                                    >  1:3033285/1‑100 (MQ=255)
                                              cTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCgcggcggc                                                   >  1:3595153/1‑99 (MQ=255)
                                                cACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGgcgggggccg                                                 >  2:3565587/1‑95 (MQ=255)
                                                cACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGGGGCgg                                                 >  1:79966/1‑101 (MQ=255)
                                                 cccgccgCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGc                                                <  2:706775/100‑1 (MQ=255)
                                                  ccgccgCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGGGGCGGcc                                               >  1:1601787/1‑101 (MQ=255)
                                                    gccgcAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTc                                             <  2:3932456/101‑1 (MQ=255)
                                                    gccgcAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTc                                             <  2:1636548/101‑1 (MQ=255)
                                                     ccgcAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGccccc                                            >  1:483759/1‑98 (MQ=255)
                                                      cgcAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGCGGCCGCCCCgc                                           >  1:3346229/1‑100 (MQ=255)
                                                       gcAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCTAc                                          <  1:2718264/101‑1 (MQ=255)
                                                           cggcggCAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTg                                      <  1:620988/101‑1 (MQ=255)
                                                            ggcggcAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTgg                                     <  2:4573075/101‑1 (MQ=255)
                                                              cggcAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGAt                                   <  1:1962514/101‑1 (MQ=255)
                                                               ggcAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATg                                  <  1:1272338/101‑1 (MQ=255)
                                                               ggcAACTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATg                                  <  1:4348117/101‑1 (MQ=255)
                                                                  aaCTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGCGGCCGGCCCCACGCCGGATGACg                               >  2:3791768/1‑101 (MQ=255)
                                                                   aCTCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGt                              <  1:2461421/101‑1 (MQ=255)
                                                                     tCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCa                            <  2:3779655/101‑1 (MQ=255)
                                                                     tCAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCTCGCCGCCGGGTGACGGCa                            >  2:2165479/1‑101 (MQ=255)
                                                                      cAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCaa                           <  1:110003/101‑1 (MQ=255)
                                                                      cAGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCaa                           <  2:1124753/101‑1 (MQ=255)
                                                                       aGGAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAg                          <  2:2089518/101‑1 (MQ=255)
                                                                        ggAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAgg                         <  2:4338887/101‑1 (MQ=255)
                                                                        ggAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAgg                         <  1:1261454/101‑1 (MQ=255)
                                                                        ggAAGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCCCGACGCCGGATGACGGCAAgg                         >  2:1503806/1‑101 (MQ=255)
                                                                          aaGAGGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGcc                       <  2:2835983/101‑1 (MQ=255)
                                                                           agagGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGccc                      <  2:3529217/101‑1 (MQ=255)
                                                                           agagGTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCTCGGCCCCGGATGACGTCAAGGgcc                      >  2:962026/1‑101 (MQ=255)
                                                                              ggTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCcgcg                   <  1:2321822/101‑1 (MQ=255)
                                                                               gTAAACATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCCGGGGCGGCCCCGGCGCCGGGTGACGGCAAAGCCCCCgg                  >  1:973144/1‑101 (MQ=255)
                                                                                 aaaCATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGcc                <  1:872272/101‑1 (MQ=255)
                                                                                 aaaCATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCCTCACGCCGGGTGACGTCACACGCCGCGGcc                >  2:3625337/1‑101 (MQ=255)
                                                                                  aaCATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGCGGCGGCCCCGACGCTGGATGACGGCAAGGCCCCCGGccc               >  1:37134/1‑100 (MQ=255)
                                                                                    cATGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGGCTCGACGCTGGGTGACGTCCAGGGCCGCGGCCCGc             >  2:3685239/1‑101 (MQ=255)
                                                                                     aTGAAAAAATCCGTATTCTTCGGCTCGGGCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCCCGACCCTGGCTGACGTCAAGGGCCGCGGCCCGCt            >  2:861844/1‑101 (MQ=255)
                                                                                      tGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCTCGCCGCCGGATGACGGCAAGGCCCCCGGCCAGccg           >  1:4133891/1‑99 (MQ=255)
                                                                                      tGAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCTCGACGCCGGATGACGTCAAGGGCCCCGGCCAGccg           >  2:3971789/1‑99 (MQ=255)
                                                                                       gAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGCGGCGGCCTCGACGCTGGGTGACGTCAAGGCCCGCGGCCCGCTGa          >  1:3236975/1‑101 (MQ=255)
                                                                                       gAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGGGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGa          >  1:3409246/1‑101 (MQ=255)
                                                                                       gAAAAAATCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCCGGATGACGTCAAGGCCCCCgggccgcgga          >  1:317441/1‑93 (MQ=255)
                                                                                        aaaaaaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGGTGACGTCAAGGGCCGCGGCCAGCCGAt         >  2:24830/1‑101 (MQ=255)
                                                                                        aaaaaaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGAt         <  2:3472697/101‑1 (MQ=255)
                                                                                         aaaaaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCTCGACGCTGGATGACGGCAAGGCCCCCGGCCCGCTGAtt        >  2:2616787/1‑100 (MQ=255)
                                                                                          aaaaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGATCt       <  1:72471/101‑1 (MQ=255)
                                                                                           aaaTCCGTATTCTTCGGCTCGGTCGCACTGACCGCCCTCGTGGCGGGGGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGATCTg      <  1:3910467/101‑1 (MQ=255)
                                                                                            aaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGGCGCCGCCCCGCCGGTGGAAGACGCCAAGGGCCCCGGCCCGCGGATCttg     >  1:1068706/1‑99 (MQ=255)
                                                                                            aaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGCCGCTGGGTGACGGCAAGGGCCGCGGCCAGGTGATCTGc     >  2:894123/1‑101 (MQ=255)
                                                                                            aaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGGTGACGTCAAGGGCCGCGGGCAGCTGATCTGc     >  2:2742578/1‑101 (MQ=255)
                                                                                            aaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCCGGGTGACGTCAAGGCCCGCGGCCAGCTGATCTGc     >  1:3083300/1‑101 (MQ=255)
                                                                                            aaTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGGCGGCCCCGACGCTGGGTGACGTTAAGGCCCCCGGCCAGCTGATTTGc     >  1:2062595/1‑101 (MQ=255)
                                                                                             aTCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGGGTCGGCCTCGCCGCCGGGTGACGGCAAGGCCCGCGGCCCGCTGATCTGCg    >  1:3451474/1‑101 (MQ=255)
                                                                                              tCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGCGTCGGCCTCGACGCTGGATGACGTCAACGCCCCCGGCCAGCTGATCTGGgg   >  2:4693492/1‑101 (MQ=255)
                                                                                              tCCGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGATCTGCgg   <  1:2611537/101‑1 (MQ=255)
                                                                                               ccGTATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGGGCGGCGGCGGCCCCGACGCTGGGTGACGTCAAGGCCCGCGGCCCGCCGATCTGCGGc  >  1:2541743/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
AGCCGGTGATGATCGGCACAGAACTTGCAGACCCGTCAAGGGCAGACTCACCGCCGCAACGGCGGCAACTCAGGAAGAGGTAAACATGAAAAAATCCGCATTCTTCGGCTCGGTCGCACTGGCCGCCCTCGTGGCGGGCGCGGCGTCGGCCTCGACGCTGGATGACGTCAAGGCCCGCGGCCAGCTGATCTGCGGC  >  NC_014034/362822‑363017

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG