Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 367,286 G→A 100% A214T (GCC→ACC)  bztD → glutamate/aspartate ABC transporter ATP‑binding protein BztD

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034367,2860G→A100.0% 1006.8 269A214T (GCC→ACC) bztDglutamate/aspartate ABC transporter ATP‑binding protein BztD
Reads supporting (aligned to +/- strand):  new base (148/117):  ref base (0/2):  total (148/121)

Only 100 of 281 total aligned reads displayed.

TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCGCAA  >  NC_014034/367188‑367386
                                                                                                  |                                                                                                    
 cgacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAccc                                                                                                   >  1:1418734/1‑101 (MQ=255)
 cgacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCAccc                                                                                                   >  2:4445139/1‑101 (MQ=255)
  gacgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCa                                                                                                  >  1:2097524/1‑101 (MQ=255)
    cgaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATg                                                                                                <  2:1526260/101‑1 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                               >  1:3231452/1‑101 (MQ=255)
     gaACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATga                                                                                               <  1:614320/101‑1 (MQ=255)
      accccACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATgag                                                                                              <  1:3866431/99‑1 (MQ=255)
             cTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCt                                                                                       >  2:3329270/1‑101 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCtt                                                                                      >  1:3559065/1‑101 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCtt                                                                                      <  2:3801604/101‑1 (MQ=255)
              tCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCtt                                                                                      <  2:1339721/101‑1 (MQ=255)
               cGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCttt                                                                                     >  2:2146939/1‑101 (MQ=255)
                ggCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTg                                                                                    >  2:359511/1‑101 (MQ=255)
                    tttGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCa                                                                                >  1:3143676/2‑101 (MQ=255)
                     ttGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAg                                                                               <  1:3776058/101‑1 (MQ=255)
                     ttGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAg                                                                               <  1:3672665/101‑1 (MQ=255)
                      tGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCtttagatcgg                                                                              >  1:2166536/1‑94 (MQ=255)
                      tGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAgg                                                                              >  2:3206102/1‑101 (MQ=255)
                       gACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGc                                                                             >  1:175451/1‑101 (MQ=255)
                        aCCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCg                                                                            <  2:263423/101‑1 (MQ=255)
                            cGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTgg                                                                        <  2:3717312/101‑1 (MQ=255)
                            cGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTgg                                                                        <  1:1035855/101‑1 (MQ=255)
                             gagaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGc                                                                       >  1:259984/1‑101 (MQ=255)
                              agaTGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCg                                                                      >  1:1825039/1‑101 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGaa                                                                    <  2:2065118/101‑1 (MQ=255)
                                atgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGaa                                                                    >  2:4697582/1‑101 (MQ=255)
                                 tgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAc                                                                   <  2:2045918/101‑1 (MQ=255)
                                 tgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAc                                                                   >  2:1265182/1‑101 (MQ=255)
                                 tgatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAc                                                                   >  2:518935/1‑101 (MQ=255)
                                  gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGTCGGTGGCGAAcc                                                                  >  1:1871538/1‑101 (MQ=255)
                                  gatCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAcc                                                                  >  1:3756601/1‑101 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                                >  1:4708056/1‑101 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                                >  2:4492275/1‑101 (MQ=255)
                                    tCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCgg                                                                >  2:94368/1‑101 (MQ=255)
                                     cAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGAATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCggg                                                               >  2:1050068/1‑101 (MQ=255)
                                      aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGt                                                              >  2:1588003/1‑101 (MQ=255)
                                      aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGt                                                              <  1:784484/101‑1 (MQ=255)
                                      aaGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGCCTTTGCCCAGGCGGGGGCGAACAgggg                                                              >  2:769249/1‑100 (MQ=255)
                                       aGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTg                                                             >  1:3393622/1‑101 (MQ=255)
                                       aGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTg                                                             <  2:4603632/101‑1 (MQ=255)
                                       aGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTg                                                             <  1:1907969/101‑1 (MQ=255)
                                         gAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGAt                                                           >  1:2813463/1‑101 (MQ=255)
                                         gAAGTGCTGGAAACGATGATCCCGCGCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGAt                                                           <  1:4659431/101‑1 (MQ=255)
                                          aaGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATc                                                          >  2:3977278/1‑101 (MQ=255)
                                            gTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGAATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCtt                                                        <  1:1050147/101‑1 (MQ=255)
                                             tGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTc                                                       >  2:2271916/1‑101 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                      <  2:241993/101‑1 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                      <  1:2039129/101‑1 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                      <  2:3575390/101‑1 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                      >  2:1190959/1‑101 (MQ=255)
                                              gCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCa                                                      <  2:3828939/101‑1 (MQ=255)
                                                tGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATg                                                    >  1:3962359/1‑101 (MQ=255)
                                                 ggATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATgg                                                   >  2:1514045/1‑101 (MQ=255)
                                                   aTACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGcc                                                 >  1:169945/1‑101 (MQ=255)
                                                    tACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACAGGGTGATCTTCATGGCCg                                                >  2:4337150/1‑101 (MQ=255)
                                                      cGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAAACGGGTGATCTTCATGGCCGAc                                              >  2:2535794/1‑101 (MQ=255)
                                                       gatgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACg                                             <  2:1705231/101‑1 (MQ=255)
                                                        atgatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACgg                                            <  1:486164/101‑1 (MQ=255)
                                                          gatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGc                                          >  1:953230/1‑101 (MQ=255)
                                                          gatCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGc                                          <  2:1904576/101‑1 (MQ=255)
                                                             ccAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGa                                       <  1:1575933/101‑1 (MQ=255)
                                                              cAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAt                                      <  2:2898590/101‑1 (MQ=255)
                                                               aGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtc                                     >  1:655838/1‑101 (MQ=255)
                                                                gCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcg                                    >  2:11172/1‑101 (MQ=255)
                                                                 cTCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgt                                   >  1:427556/1‑101 (MQ=255)
                                                                  tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                                  <  1:2870986/101‑1 (MQ=255)
                                                                  tCGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtc                                  <  1:834435/101‑1 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                                 <  2:3453857/101‑1 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                                 >  2:3128534/1‑101 (MQ=255)
                                                                   cGCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGAtcgtcg                                 <  2:864062/101‑1 (MQ=255)
                                                                    gCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGa                                <  2:623750/101‑1 (MQ=255)
                                                                    gCCGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGa                                >  1:1200066/1‑101 (MQ=255)
                                                                      cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                              <  2:4085590/101‑1 (MQ=255)
                                                                      cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                              >  1:3345909/1‑101 (MQ=255)
                                                                      cGAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGc                              >  1:205578/1‑101 (MQ=255)
                                                                       gAGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCa                             >  1:4449593/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                            >  1:2447075/1‑101 (MQ=255)
                                                                        aGGAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAg                            <  2:4025665/101‑1 (MQ=255)
                                                                          gAAGGCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaa                          >  1:1286585/1‑101 (MQ=255)
                                                                              gCATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGaacaac                      <  2:4469261/101‑1 (MQ=255)
                                                                               cATGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                     <  2:552698/101‑1 (MQ=255)
                                                                               cATGACGATGCTCTGCGTCACCCATGAGATGGGCCTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAcc                     <  1:111880/101‑1 (MQ=255)
                                                                                aggACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAAccc                    <  2:3295702/99‑1 (MQ=255)
                                                                                aTGACGATGCTCTGCGT.ACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCg                   >  2:2699329/1‑101 (MQ=255)
                                                                                 tGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCg                   >  1:2643182/1‑101 (MQ=255)
                                                                                 tGACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCg                   <  1:295952/101‑1 (MQ=255)
                                                                                  gACGATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGc                  >  1:2721214/1‑101 (MQ=255)
                                                                                     gATGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATg               >  1:4506553/1‑101 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa              >  2:1798956/1‑101 (MQ=255)
                                                                                      aTGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGa              <  1:223100/101‑1 (MQ=255)
                                                                                       tGCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAc             <  2:393046/101‑1 (MQ=255)
                                                                                        gCTCTGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGAct            >  1:3119099/1‑101 (MQ=255)
                                                                                         ctctGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGActt           <  1:401657/101‑1 (MQ=255)
                                                                                          catgtgTCACCCAAGAGATGGCCTTTGCCCAGGCGGTGGGGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACACCCCGCATGActcc          <  2:2199005/96‑3 (MQ=37)
                                                                                            tGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCCGATCGTCGAGCAGAACAACCCGCATGActtctt        >  1:4113748/1‑101 (MQ=255)
                                                                                            tGCGTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCCGAACAACCCGCATGActtctt        <  1:3494851/101‑1 (MQ=255)
                                                                                              cGTCACCCATGAGGTGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcc      <  1:2348591/101‑1 (MQ=255)
                                                                                               gTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcca     >  1:151377/1‑100 (MQ=255)
                                                                                               gTCACCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCCGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTcca     <  2:4747938/101‑2 (MQ=255)
                                                                                                  gCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGACCCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCACaa  <  1:4076930/101‑1 (MQ=255)
                                                                                                  |                                                                                                    
TCGACGAACCCACCTCGGCGCTTGACCCCGAGATGATCAAGGAAGTGCTGGATACGATGATCCAGCTCGCCGAGGAAGGCATGACGATGCTCTGCGTCGCCCATGAGATGGGCTTTGCCCAGGCGGTGGCGAACCGGGTGATCTTCATGGCCGACGGGCAGATCGTCGAGCAGAACAACCCGCATGACTTCTTCCGCAA  >  NC_014034/367188‑367386

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG