Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 419,739 C→A 100% T591N (ACC→AAC)  RCAP_rcc00388 → OmpA/MotB domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034419,7390C→A100.0% 791.5 203T591N (ACC→AAC) RCAP_rcc00388OmpA/MotB domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (121/82):  ref base (0/0):  total (121/82)

Only 100 of 217 total aligned reads displayed.

TTCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGACCCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGCCCGCG  >  NC_014034/419641‑419836
                                                                                                  |                                                                                                 
ttCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAacc                                                                                                 >  1:2487342/1‑101 (MQ=255)
tccgatctgcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTGTGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAacc                                                                                                 <  1:973652/93‑1 (MQ=255)
 tCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCg                                                                                                >  2:3843252/1‑101 (MQ=255)
  ccGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCgg                                                                                               >  1:1528712/1‑101 (MQ=255)
       cgcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCAt                                                                                          <  1:759359/101‑1 (MQ=255)
        gcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATc                                                                                         <  2:337465/101‑1 (MQ=255)
        gcCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATc                                                                                         <  1:377332/101‑1 (MQ=255)
          cGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGa                                                                                       >  1:909871/1‑101 (MQ=255)
            ggTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAAt                                                                                     >  2:4461904/1‑101 (MQ=255)
            ggTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAAt                                                                                     >  1:1079489/1‑101 (MQ=255)
            ggTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGCCGCATCGAAt                                                                                     >  2:250835/1‑101 (MQ=255)
              tGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTc                                                                                   <  1:2858552/101‑1 (MQ=255)
                 tGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGtg                                                                                >  1:4488767/1‑101 (MQ=255)
                    cgcgCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGtgctg                                                                             <  1:1747503/101‑1 (MQ=255)
                      cgcAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTgctgct                                                                           <  1:796628/101‑1 (MQ=255)
                        cAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTgg                                                                         >  2:2220891/1‑101 (MQ=255)
                           ccTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATg                                                                      <  2:1192261/101‑1 (MQ=255)
                           ccTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATg                                                                      <  2:4624524/101‑1 (MQ=255)
                            cTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGc                                                                     >  1:1968926/1‑101 (MQ=255)
                             tGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGcc                                                                    >  2:1757951/1‑101 (MQ=255)
                             tGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGcc                                                                    >  1:2805843/1‑101 (MQ=255)
                             tGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGcc                                                                    <  1:3574895/101‑1 (MQ=255)
                               aCGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCaa                                                                  <  1:4262241/101‑1 (MQ=255)
                                cGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAg                                                                 <  2:105165/101‑1 (MQ=255)
                                cGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAg                                                                 >  2:4078934/1‑101 (MQ=255)
                                cGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAg                                                                 <  2:1464855/101‑1 (MQ=255)
                                 gCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGc                                                                <  2:2754802/101‑1 (MQ=255)
                                  cccAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGcc                                                               <  1:2590962/101‑1 (MQ=255)
                                    cAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCgg                                                             >  1:3071641/1‑101 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                            >  2:2436694/1‑101 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                            >  2:1872792/1‑101 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                            >  1:239008/1‑101 (MQ=255)
                                     aaGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGt                                                            >  1:177456/1‑101 (MQ=255)
                                      aGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTg                                                           <  1:3669691/101‑1 (MQ=255)
                                      aGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTg                                                           <  1:4428893/101‑1 (MQ=255)
                                      aGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTg                                                           <  2:1300447/101‑1 (MQ=255)
                                       gggCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgg                                                          >  1:4746803/1‑101 (MQ=255)
                                       gggCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgg                                                          <  2:3278448/101‑1 (MQ=255)
                                        ggCTATGGGGAAAGCCATCCCAGCGCCGAGAATGACACCGCCAAGGGCCGCGAGGCGAACCGGCGCACCGAATTCGTGCTGCTGGATGCCAAGCCGGTggg                                                         <  2:4224128/101‑1 (MQ=255)
                                        ggCTATGGGGAAAACCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGGGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTggg                                                         <  1:2187113/101‑1 (MQ=255)
                                        ggCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTggg                                                         >  2:2670184/1‑101 (MQ=255)
                                        ggCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTggg                                                         <  2:314114/101‑1 (MQ=255)
                                         gCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggg                                                        >  2:2099345/1‑101 (MQ=255)
                                          cTATGGCGAAAGCCAGCCCATAGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGGGCTGCTGTATGCCAAGCCGGTggggg                                                       >  2:1564131/1‑101 (MQ=255)
                                           tATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggggg                                                      >  2:3979087/1‑101 (MQ=255)
                                           tATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTgggggg                                                      <  1:3321541/101‑1 (MQ=255)
                                             tGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgcg                                                    <  2:4565388/101‑1 (MQ=255)
                                             tGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgcg                                                    <  2:2487198/101‑1 (MQ=255)
                                             tGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGgcg                                                    >  2:1820389/1‑101 (MQ=255)
                                               gCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGcc                                                  >  1:2773960/1‑101 (MQ=255)
                                               gCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGcc                                                  >  1:2029682/1‑101 (MQ=255)
                                                 gAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCgg                                                <  2:3189577/101‑1 (MQ=255)
                                                    agccagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCa                                             <  1:4540942/101‑1 (MQ=255)
                                                     gccagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAg                                            >  1:4086977/1‑101 (MQ=255)
                                                     gccagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGCGGGGGGCGCCGGGCAg                                            >  1:4115037/1‑101 (MQ=255)
                                                       cagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgat                                          >  1:2190993/1‑101 (MQ=255)
                                                       cagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgat                                          >  2:2857559/1‑101 (MQ=255)
                                                       cagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgat                                          >  1:503616/1‑101 (MQ=255)
                                                       cagccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgat                                          <  1:4388887/101‑1 (MQ=255)
                                                        agccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatc                                         >  2:354814/1‑101 (MQ=255)
                                                        agccCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatc                                         <  2:845294/101‑1 (MQ=255)
                                                          cccATCGCCGAGAATTACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatcga                                       <  1:250860/101‑1 (MQ=255)
                                                            cATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAgatcgatc                                     >  1:1227868/1‑101 (MQ=255)
                                                              tCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTg                                   <  1:3501780/101‑1 (MQ=255)
                                                               cGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGc                                  <  1:1644930/101‑1 (MQ=255)
                                                               cGCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGc                                  >  1:138613/1‑101 (MQ=255)
                                                                gCCGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGcc                                 >  1:1265795/1‑101 (MQ=255)
                                                                 ccGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCAGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCg                                <  2:4103049/101‑1 (MQ=255)
                                                                 cagagaATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCg                                <  1:2894359/99‑1 (MQ=255)
                                                                  cGAGAATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGTCGCCGGGCAGATCGATCTGCCgg                               >  1:42051/1‑101 (MQ=255)
                                                                    agaATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTg                             <  2:3867500/101‑1 (MQ=255)
                                                                    agaATGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTg                             >  2:766220/1‑101 (MQ=255)
                                                                      aaTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGAc                           >  2:582387/1‑101 (MQ=255)
                                                                       aTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACg                          <  2:1410278/101‑1 (MQ=255)
                                                                       aTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACg                          >  2:2396351/1‑101 (MQ=255)
                                                                       aTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACg                          <  1:3931984/101‑1 (MQ=255)
                                                                       aTGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACg                          >  2:2782571/1‑101 (MQ=255)
                                                                        tGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCGGATCGATCTGCCGGCGAGgg                         >  2:1660167/1‑101 (MQ=255)
                                                                        tGACACCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACgg                         <  1:1361810/101‑1 (MQ=255)
                                                                          acacCGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTg                       <  2:46137/101‑1 (MQ=255)
                                                                             ccGACAAGGGCCGCGGGGGGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCg                    <  1:3283866/101‑1 (MQ=255)
                                                                             ccGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCg                    <  1:1063399/101‑1 (MQ=255)
                                                                              cGACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCgg                   <  2:2645714/101‑1 (MQ=255)
                                                                               gACAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGa                  >  1:78139/1‑101 (MQ=255)
                                                                                aCAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGaa                 <  1:2099466/101‑1 (MQ=255)
                                                                                aCAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGaa                 >  1:2688339/1‑101 (MQ=255)
                                                                                 cAAGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAc                >  1:18251/1‑101 (MQ=255)
                                                                                  aggggCCGCGGGGCGAACGTGCGCATCGAGTTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAcc               <  2:4380827/99‑1 (MQ=255)
                                                                                  aaGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAcc               >  2:532890/1‑101 (MQ=255)
                                                                                   aGGGCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACCGTGCCGGAAccg              <  1:2574131/101‑1 (MQ=255)
                                                                                    gggCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAccgc             <  1:3364329/101‑1 (MQ=255)
                                                                                    gggCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAccgc             <  1:1923700/101‑1 (MQ=255)
                                                                                      gCCGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAAccgccg           >  1:441980/1‑101 (MQ=255)
                                                                                       ccGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGa          >  2:3092637/1‑101 (MQ=255)
                                                                                       ccGCGAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGa          <  1:2436829/101‑1 (MQ=255)
                                                                                        cgcgAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGaa         <  2:503565/101‑1 (MQ=255)
                                                                                           gAGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGcc      >  1:1789629/1‑101 (MQ=255)
                                                                                            aGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGccc     >  2:2600885/1‑101 (MQ=255)
                                                                                            aGGCGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGccc     <  1:4604428/101‑1 (MQ=255)
                                                                                               cGAACCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGCCcgcc  >  2:4529949/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
TTCCGCGCGCCGGGTGCTGACGCGCAACCTGACGCCCAAGGGCTATGGCGAAAGCCAGCCCATCGCCGAGAATGACACCGACAAGGGCCGCGAGGCGACCCGGCGCATCGAATTCGTGCTGCTGGATGCCAAGCCGGTGGGGGGCGCCGGGCAGATCGATCTGCCGGTGACGGTGCCGGAACCGCCGAAGCCCGCG  >  NC_014034/419641‑419836

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG