Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 429,595 T→G 100% R133R (AGG→CGG)  pyrB ← aspartate carbamoyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034429,5950T→G100.0% 737.3 198R133R (AGG→CGG) pyrBaspartate carbamoyltransferase
Reads supporting (aligned to +/- strand):  new base (92/106):  ref base (0/0):  total (92/106)

Only 100 of 208 total aligned reads displayed.

GACATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCTCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATGC  >  NC_014034/429497‑429692
                                                                                                  |                                                                                                 
gACATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGcc                                                                                                 >  2:2671506/1‑101 (MQ=255)
 aCATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGccc                                                                                                >  1:2264492/1‑101 (MQ=255)
  cagcgccgcAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCg                                                                                               >  2:1284540/4‑101 (MQ=255)
   aTCGCCGCAAATCGCCACCGTCAGCCGCTGGGTCCGGCCCTTGGCACGCCGGATCGGCAAGGCATCCAAAAGCGCCCGCGTCGGGCGCTCGGGCCGCCCGt                                                                                              >  2:3855890/1‑101 (MQ=255)
         gcAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCg                                                                                        <  2:2126755/101‑1 (MQ=255)
          cAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGc                                                                                       <  1:3056932/101‑1 (MQ=255)
          cAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGc                                                                                       <  1:1522064/101‑1 (MQ=255)
           aaaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCa                                                                                      <  1:1986781/101‑1 (MQ=255)
           aaaTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGGGTTCGTGCCGCCCGTCGCCCGCa                                                                                      >  2:2331530/1‑101 (MQ=255)
             aTCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCAtt                                                                                    >  1:3184636/1‑101 (MQ=255)
               cGCCACCGTCAGCCGCTGGATCCAGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGa                                                                                  <  2:3304561/101‑1 (MQ=255)
                gCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGACGGGTGTTCGTGCCGCCCGTCGCCCGCATTGAt                                                                                 >  1:4036336/1‑101 (MQ=255)
                 ccACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGTTc                                                                                <  1:3466366/101‑1 (MQ=255)
                  cACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATca                                                                               >  2:3020738/1‑101 (MQ=255)
                  cACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATca                                                                               >  2:697392/1‑101 (MQ=255)
                    ccGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTCTTCGTGCCGCCCGTCGCCCGCATTGATcaca                                                                             <  1:4209060/101‑1 (MQ=255)
                     cGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAg                                                                            <  2:4230826/101‑1 (MQ=255)
                      gTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGc                                                                           <  1:3481041/101‑1 (MQ=255)
                        cAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGcac                                                                         >  1:3738741/1‑101 (MQ=255)
                         aGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGcaca                                                                        <  1:4672391/101‑1 (MQ=255)
                         aGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGcaca                                                                        >  2:419050/1‑101 (MQ=255)
                          gCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACaa                                                                       >  1:4253865/1‑101 (MQ=255)
                           ccGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAAt                                                                      <  1:1277579/101‑1 (MQ=255)
                            cGCTGGATCCGGCCCTTGGCACGCCGGAGCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAAtt                                                                     <  2:1361066/101‑1 (MQ=255)
                             gCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTc                                                                    <  1:1864954/101‑1 (MQ=255)
                              cTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAgatcggaaga                                                                   >  2:598221/1‑92 (MQ=255)
                              cTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCa                                                                   <  1:2942383/101‑1 (MQ=255)
                              cTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCa                                                                   <  1:2661743/101‑1 (MQ=255)
                               tGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTcgc                                                                  <  1:1584060/101‑3 (MQ=255)
                               tGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCAc                                                                  >  1:1158428/1‑101 (MQ=255)
                                ggATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCAcc                                                                 >  2:4046440/1‑101 (MQ=255)
                                 gATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCt                                                                <  2:291124/101‑1 (MQ=255)
                                 gATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCt                                                                >  1:4369918/1‑101 (MQ=255)
                                 gATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCt                                                                >  2:1869019/1‑101 (MQ=255)
                                 gATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCt                                                                <  1:104579/101‑1 (MQ=255)
                                   tCCGGCCCTTGGCACGCCCGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTc                                                              <  1:3934806/101‑1 (MQ=255)
                                     cGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTg                                                            <  1:1033800/101‑1 (MQ=255)
                                        cccTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTgcgc                                                         <  1:2761712/101‑1 (MQ=255)
                                         ccTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGcc                                                        <  1:2153617/101‑1 (MQ=255)
                                          cTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCa                                                       <  2:2264359/101‑1 (MQ=255)
                                           ttGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCag                                                      <  1:697456/101‑1 (MQ=255)
                                            tGGCACGCCGGATCGTCAGGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCaga                                                     >  1:2949570/1‑101 (MQ=255)
                                            tGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCaga                                                     <  1:4341403/101‑1 (MQ=255)
                                            tGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCaga                                                     <  2:3329495/101‑1 (MQ=255)
                                             ggCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCagaa                                                    <  2:192247/101‑1 (MQ=255)
                                               cACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCagaaga                                                  <  2:504182/101‑1 (MQ=255)
                                                aCGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGAt                                                 >  1:3658475/1‑101 (MQ=255)
                                                 cGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATc                                                <  1:4722103/101‑1 (MQ=255)
                                                  gCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCg                                               >  2:2205822/1‑101 (MQ=255)
                                                  gCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCg                                               >  2:1095183/1‑101 (MQ=255)
                                                    cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCTCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             <  1:3856132/101‑1 (MQ=255)
                                                    cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             <  2:2782533/101‑1 (MQ=255)
                                                    cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             <  2:2410280/101‑1 (MQ=255)
                                                    cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             <  2:1521807/101‑1 (MQ=255)
                                                    cGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGAc                                             >  2:1702013/1‑101 (MQ=255)
                                                     ggATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACg                                            <  2:2892687/101‑1 (MQ=255)
                                                       aTCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGgc                                          <  1:2525625/101‑1 (MQ=255)
                                                        tCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGgcg                                         >  1:2452453/1‑101 (MQ=255)
                                                          gTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGcc                                       >  2:388044/1‑101 (MQ=255)
                                                          gTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGcc                                       >  1:443550/1‑101 (MQ=255)
                                                          gTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGcc                                       >  2:4488622/1‑101 (MQ=255)
                                                           tCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCg                                      <  2:1582132/101‑1 (MQ=255)
                                                           tCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCg                                      <  2:2017872/101‑1 (MQ=255)
                                                           tCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCg                                      <  1:1302877/101‑1 (MQ=255)
                                                            cAAGGCATCCAAAAGGCCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCgg                                     <  2:4362977/101‑1 (MQ=255)
                                                            cAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCgg                                     >  1:3371982/1‑101 (MQ=255)
                                                              aGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggcg                                   >  2:1176071/1‑99 (MQ=255)
                                                              aGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggag                                   >  2:636226/1‑101 (MQ=255)
                                                                gCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggagga                                 >  1:841683/1‑101 (MQ=255)
                                                                gCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggagga                                 <  1:2331663/101‑1 (MQ=255)
                                                                gCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCggagga                                 <  1:2311157/101‑1 (MQ=255)
                                                                 cATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGgaggag                                >  2:4291393/1‑101 (MQ=255)
                                                                  aTCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGaggagg                               >  1:3609353/1‑101 (MQ=255)
                                                                   tCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAggg                              <  1:2789285/101‑1 (MQ=255)
                                                                    ccAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGa                             >  2:1368228/1‑101 (MQ=255)
                                                                     cAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGAt                            <  1:3320708/101‑1 (MQ=255)
                                                                     cAAAAGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGAt                            <  2:2571319/101‑1 (MQ=255)
                                                                      aaaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATg                           >  2:3728457/1‑101 (MQ=255)
                                                                      aaaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATg                           <  1:410102/101‑1 (MQ=255)
                                                                      aaaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGACCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATg                           >  2:1804361/1‑101 (MQ=255)
                                                                       aaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGc                          >  2:3178695/1‑101 (MQ=255)
                                                                       aaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGc                          >  2:4546618/1‑101 (MQ=255)
                                                                       aaaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGc                          <  1:938656/101‑1 (MQ=255)
                                                                        aaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGcc                         >  2:3685746/1‑101 (MQ=255)
                                                                        aaGCGCCTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGcc                         <  2:4095286/101‑1 (MQ=255)
                                                                             ccTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcacc                    >  2:1633237/1‑101 (MQ=255)
                                                                              cTGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGcacca                   >  2:3109428/1‑101 (MQ=255)
                                                                               tGCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGCTGCCGcaccac                  >  2:2616326/1‑101 (MQ=255)
                                                                                gCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCaccacc                 >  1:1734366/1‑101 (MQ=255)
                                                                                gCGTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCaccacc                 <  2:3636297/101‑1 (MQ=255)
                                                                                  tctgggTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcag               <  2:792232/98‑1 (MQ=255)
                                                                                  gTCGGGTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcag               >  2:4716654/1‑101 (MQ=255)
                                                                                     gggTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcagcag            >  1:858115/1‑101 (MQ=255)
                                                                                     gggTGTTCGAGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACcagcag            <  1:2624204/101‑1 (MQ=255)
                                                                                      ggTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGa           >  2:4750301/1‑101 (MQ=255)
                                                                                      ggTGTTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGa           <  1:1368322/101‑1 (MQ=255)
                                                                                       gtgtTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGAt          >  2:4606766/1‑101 (MQ=255)
                                                                                       gtgtTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGAt          <  1:3685975/101‑1 (MQ=255)
                                                                                         gtTCGTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCg        <  1:1811262/101‑1 (MQ=255)
                                                                                             gTGCCGCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGAt    <  1:1095269/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
GACATCGCCGCAAATCGCCACCGTCAGCCGCTGGATCCGGCCCTTGGCACGCCGGATCGTCAAGGCATCCAAAAGCGCCTGCGTCGGGTGTTCGTGCCTCCCGTCGCCCGCATTGATCACAGCACAATTCACCTTCTGCGCCAGAAGATCGACGGCGCCGGAGGAGGGATGCCGCACCACCAGCAGATCGGGATGC  >  NC_014034/429497‑429692

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 40 ≤  ATCG