Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,503 G→A 2.9% intergenic (+106/+42) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,5030G→A 2.9% 6.8 ‑3.4 ‑0.0206intergenic (+106/+42)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (6/0):  ref base (108/91):  total (115/91)
Fisher's exact test strand distribution p-value = 3.48e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 223 total aligned reads displayed.

GGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCG  >  NC_014034/432403‑432602
                                                                                                      |                                                                                                   
ggacggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGA.TTTCATCTTGCCCGAAATATCCCAGGGGTGAAtt                                                                                           >  2:3830301/5‑101 (MQ=2)
  cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCgg                                                                                                    <  2:4710265/101‑1 (MQ=255)
  cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCgg                                                                                                    >  2:1655298/1‑101 (MQ=255)
  cAGGGGGGCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.GTTTATCTTGCCCCCAATATCCCgg                                                                                                    >  1:1016629/1‑101 (MQ=21)
  acggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGA.TTTCATCTTGCCCGAAATATCCCAGGGGTGAAttgg                                                                                         >  2:4747599/3‑99 (MQ=2)
   cggggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGA.TTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggc                                                                                        >  1:4450050/2‑98 (MQ=2)
     gggggCTCTGCCCCCCGG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGA.TTTCATCTTGCCCGAAATATCCCAGGGGTGAAttggcca                                                                                      >  1:2340374/1‑96 (MQ=2)
      ggggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGt                                                                                                <  1:1398620/101‑1 (MQ=255)
       gggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTg                                                                                               >  1:4591989/1‑101 (MQ=255)
        ggCGCTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGa                                                                                              <  1:2948303/101‑1 (MQ=255)
         gcgcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGaa                                                                                             <  1:364557/101‑1 (MQ=255)
           gcTGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAAtt                                                                                           >  1:641755/1‑101 (MQ=255)
             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                         >  2:3028665/1‑101 (MQ=255)
             tGCCCCCCGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAAtttt                                                                                         >  2:3064907/1‑101 (MQ=255)
             tGCCCCACGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGAAGATACGGGATTCG.TTTCATCTTGCCCCAAATATACCGCGGGGGAAtttt                                                                                         >  2:3444843/1‑101 (MQ=18)
               ccccccGTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTgg                                                                                       <  2:1748473/101‑1 (MQ=255)
                cccccGTCCTGCGGTCTCCCCCCGGGATATTTGAGGCAAGATGAAA.CGAAGCCCGGATCGGCTTTCATCTTGCCTCAAATATCCCGGGGGTGAATTTTGgc                                                                                      >  2:2080351/1‑101 (MQ=14)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                 >  2:1037890/4‑101 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                 >  1:3051550/4‑101 (MQ=255)
                     gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                                                                 <  1:228190/98‑1 (MQ=255)
                      tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                                                >  1:4330189/3‑101 (MQ=255)
                      tccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGcc                                                                                <  2:4031372/99‑1 (MQ=255)
                       ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                               <  1:1574485/100‑1 (MQ=255)
                       ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCa                                                                               <  2:3153534/100‑1 (MQ=255)
                        cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                                              <  1:4220636/101‑1 (MQ=255)
                        cTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaa                                                                              >  1:4295049/1‑101 (MQ=255)
                        cTGCGGACTCCCCCCGGGATATTTGAGGCAAAATTAAAGCCGAACCGGGCTTCG.TTTCATCTTGCCCCCAATATTCCGGGGGTGGAGTTTTGGGCAGGCaa                                                                              >  2:4115367/1‑101 (MQ=21)
                          gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                                                            >  2:3651936/1‑101 (MQ=255)
                             gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag                                                                         <  1:816543/101‑1 (MQ=255)
                              gcTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                                                        <  2:3602634/100‑1 (MQ=255)
                               ccccccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAggg                                                                       <  2:458129/99‑1 (MQ=255)
                               cTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCAGCCAAAAGAggg                                                                       >  2:2400328/1‑101 (MQ=255)
                                tCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgggg                                                                      >  2:4081973/1‑101 (MQ=255)
                                  cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                                                    <  2:3627293/101‑1 (MQ=255)
                                  cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                                                    <  2:80764/101‑1 (MQ=255)
                                  cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                                                                    <  2:2996028/101‑1 (MQ=255)
                                   ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                                                   <  2:1124098/101‑1 (MQ=255)
                                   ccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCa                                                                   <  1:803232/101‑1 (MQ=255)
                                     ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                                                                 <  2:556818/101‑1 (MQ=255)
                                      cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                                                                >  2:1148084/1‑101 (MQ=255)
                                          atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                                                            >  2:2498536/1‑101 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                          >  2:4425583/1‑101 (MQ=255)
                                            atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                                                          <  1:4426054/101‑1 (MQ=255)
                                             tttGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                                         >  1:2641929/1‑101 (MQ=255)
                                             tttGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                                                         >  2:4285313/1‑101 (MQ=255)
                                              ttGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                                                        <  1:2192299/101‑1 (MQ=255)
                                               tGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                                       >  1:2074174/1‑101 (MQ=255)
                                               tGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                                       >  1:92859/1‑101 (MQ=255)
                                               tGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATc                                                       >  2:27802/1‑101 (MQ=255)
                                                  ggCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc                                                    >  2:2239338/1‑101 (MQ=255)
                                                   gCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg                                                   <  1:2934093/101‑1 (MQ=255)
                                                    cAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTTCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                                                  <  1:3663250/101‑1 (MQ=255)
                                                    cAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATTCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgc                                                  >  2:3987792/1‑101 (MQ=255)
                                                      aGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                                                <  1:2267930/101‑1 (MQ=255)
                                                      aGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcgg                                                >  1:3526882/1‑101 (MQ=255)
                                                       gATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc                                               >  1:1077303/1‑101 (MQ=255)
                                                        aTGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcg                                              >  1:3829740/1‑101 (MQ=255)
                                                         tGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc                                             >  1:2498727/1‑101 (MQ=255)
                                                         tGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc                                             >  1:1423441/1‑101 (MQ=255)
                                                         tGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc                                             >  2:3128865/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                            <  1:2792525/101‑1 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                            >  2:3238606/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                            <  2:3405209/101‑1 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                            >  2:3992843/1‑101 (MQ=255)
                                                          gAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg                                            >  1:3586830/1‑101 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                           >  2:1326072/1‑101 (MQ=255)
                                                           aaaGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcgg                                           >  1:2888257/1‑101 (MQ=255)
                                                            aaGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                          >  1:1732315/1‑101 (MQ=255)
                                                            aaGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggc                                          >  2:501568/1‑101 (MQ=255)
                                                              gCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcgg                                        >  2:875566/1‑101 (MQ=255)
                                                               ccGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcggggcggc                                       >  1:777452/1‑101 (MQ=255)
                                                               ccGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                                       <  1:2392117/101‑1 (MQ=255)
                                                               ccGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggc                                       <  2:4039034/101‑1 (MQ=255)
                                                                cGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg                                      >  1:3903671/1‑101 (MQ=255)
                                                                cGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCgcggcgcggcggcg                                      >  1:1206248/1‑101 (MQ=255)
                                                                   tCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGcggcgcggcggcgcgg                                   >  2:640113/1‑101 (MQ=255)
                                                                    ccGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggggcggc                                  >  1:3791429/1‑101 (MQ=255)
                                                                    ccGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCggcgcggcggcgcggc                                  <  1:1258252/101‑1 (MQ=255)
                                                                        gCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAg                              <  1:2489193/101‑1 (MQ=255)
                                                                           tCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCg                           <  2:729037/101‑1 (MQ=255)
                                                                           tCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCg                           <  1:3285927/101‑1 (MQ=255)
                                                                             g.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAACGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGt                         >  1:4019900/1‑101 (MQ=255)
                                                                               tttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTc                        <  2:4316235/101‑1 (MQ=255)
                                                                                ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg                       >  1:1913462/1‑101 (MQ=255)
                                                                                ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg                       >  2:1046949/1‑101 (MQ=255)
                                                                                ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg                       >  1:4577043/1‑101 (MQ=255)
                                                                                ttCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCg                       >  2:2866339/1‑101 (MQ=255)
                                                                                  cATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt                     >  1:3102345/1‑101 (MQ=255)
                                                                                  cATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGtt                     >  1:3756663/1‑101 (MQ=255)
                                                                                   aTCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTg                    <  1:3225020/101‑1 (MQ=255)
                                                                                     cTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGAt                  >  1:3025368/1‑101 (MQ=255)
                                                                                       tGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcg                <  2:3717584/101‑1 (MQ=255)
                                                                                       tGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcg                >  2:2166851/1‑101 (MQ=255)
                                                                                        gCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATcgc               <  2:1681371/101‑1 (MQ=255)
                                                                                            cAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCgcgcgc           >  1:2821131/1‑101 (MQ=255)
                                                                                             aaaTATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGcc          >  2:4467136/1‑101 (MQ=255)
                                                                                               atatCCCTGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCa        >  1:3184871/1‑101 (MQ=255)
                                                                                                 atCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAg      >  1:4133506/1‑101 (MQ=255)
                                                                                                     cGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCg  <  1:3029349/101‑1 (MQ=255)
                                                                                                     cGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCg  >  1:131043/1‑101 (MQ=255)
                                                                                                      |                                                                                                   
GGCAGGGGGGCGCTGCCCCCCGT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCG.TTTCATCTTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCGGCGCGGCGCAGCCGGTCGTTGATCGCGCGCCCAAGCCCG  >  NC_014034/432403‑432602

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG