Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 441,868 T→G 100% V329G (GTC→GGC)  gabD → succinate‑semialdehyde dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034441,8680T→G100.0% 719.9 190V329G (GTC→GGC) gabDsuccinate‑semialdehyde dehydrogenase
Reads supporting (aligned to +/- strand):  new base (96/94):  ref base (0/0):  total (96/94)

Only 100 of 219 total aligned reads displayed.

CCAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGTCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGGGCGGC  >  NC_014034/441770‑441965
                                                                                                  |                                                                                                 
ccAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGtcggcg                                                                                                 <  1:4340402/101‑1 (MQ=255)
 cAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGGGCGCAAGCTCAATGtcggcgg                                                                                                >  2:552743/1‑100 (MQ=255)
 cAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGGGCGCAAGCTCAATGTCGGCGa                                                                                                >  1:3456892/1‑101 (MQ=255)
  aGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGAc                                                                                               <  1:1005390/101‑1 (MQ=255)
  aGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGAc                                                                                               <  2:4316298/101‑1 (MQ=255)
  aGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGGCTATGACACTTTCGCGGAAAAGCTGGCGGCGGGGGTGGGCAAGCTCAATGGCGGCGag                                                                                               >  2:4442284/1‑100 (MQ=255)
      cTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGc                                                                                           >  2:1146004/1‑101 (MQ=255)
       tGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCt                                                                                          <  2:4232575/101‑1 (MQ=255)
       tGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCt                                                                                          <  2:3717770/101‑1 (MQ=255)
        gCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTg                                                                                         <  2:844610/101‑1 (MQ=255)
          gTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGaa                                                                                       >  1:3603786/1‑101 (MQ=255)
          gTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGaa                                                                                       >  1:2899219/1‑101 (MQ=255)
            gtGCGCGAACGGGCTCTATGTGCGGCGGGGCGGCAATGCCCCTTTCGCGGAAAAGCGGGGGGCGGCGGGCCCCAAGCCAAATGGGGGGGGGGGGCTGAAgg                                                                                     <  2:2071599/100‑1 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGa                                                                                    >  2:679787/1‑101 (MQ=255)
             tGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGa                                                                                    >  2:1779743/1‑101 (MQ=255)
              gcgcGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGATCAATGTCGGCGACGGGCTGAAGGAc                                                                                   >  1:203755/1‑101 (MQ=255)
               cgcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACg                                                                                  <  2:1488651/101‑1 (MQ=255)
                gcgAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACgg                                                                                 >  1:3896721/1‑101 (MQ=255)
                 cgAACCGGGTCTATGTGCAGGCGGGCGGCTATGACACCTTCGCGGAAAAGCTGGCGGCGGCGGTCGGCAAGGTCAATGGCGGCGACGGGCtcgacggtgtg                                                                                >  2:151275/1‑91 (MQ=255)
                 cgAACCGGATCGAGGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGAGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGc                                                                                <  1:4718656/101‑1 (MQ=255)
                  gAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCg                                                                               >  1:702375/1‑101 (MQ=255)
                   aaCCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGTGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCgg                                                                              >  2:3159900/1‑100 (MQ=255)
                   aaCCGGATCTATGTGAAGGCGGGCGTCTATGACACTTTCGCGGAAAAGATGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGAAGGCgg                                                                              >  2:304210/1‑100 (MQ=255)
                       ggATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacc                                                                          <  1:3981793/101‑1 (MQ=255)
                       ggATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacc                                                                          <  1:3495573/101‑1 (MQ=255)
                        gATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGacca                                                                         <  2:2476513/101‑1 (MQ=255)
                        gATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGacca                                                                         >  1:2735306/1‑101 (MQ=255)
                         aTCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGaccac                                                                        <  1:2796726/101‑1 (MQ=255)
                         aTCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGGGaccac                                                                        >  2:4485818/1‑101 (MQ=255)
                          tCTATGTGCGGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGAAAGCTCAAGGTCGGCGACGGGCTGAAGGACGGCGTGaccacc                                                                       <  1:4476498/101‑1 (MQ=255)
                           cTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCg                                                                      >  2:1034856/1‑101 (MQ=255)
                              tgtgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGc                                                                   <  2:1269418/101‑1 (MQ=255)
                              tgtgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGc                                                                   <  1:3711770/101‑1 (MQ=255)
                               gtgCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGGGCGGAAGGTCAATGTCGGCGCCGGGCTGAAGGACGGCGTGGCCACCGCGcc                                                                  >  2:933654/1‑101 (MQ=255)
                                 gCAGGGGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCt                                                                <  2:1895092/101‑1 (MQ=255)
                                 gCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCt                                                                <  2:386261/101‑1 (MQ=255)
                                  cAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCtt                                                               <  2:1426884/101‑1 (MQ=255)
                                   aGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTg                                                              <  2:599524/101‑1 (MQ=255)
                                      cgggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtc                                                           >  1:1586393/1‑101 (MQ=255)
                                       gggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtct                                                          <  2:203730/101‑1 (MQ=255)
                                        ggcgTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGAtctc                                                         <  1:2884067/101‑1 (MQ=255)
                                           gTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGc                                                      >  1:1616742/1‑101 (MQ=255)
                                           gTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGc                                                      >  1:11363/1‑101 (MQ=255)
                                            tCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGcc                                                     >  2:3234640/1‑101 (MQ=255)
                                               aTGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGAt                                                  >  1:1653181/1‑101 (MQ=255)
                                               aTGACACTTTCGCGGAAAAGCTGGCGGCGGCGGGGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGAt                                                  >  1:64586/1‑101 (MQ=255)
                                                tGACACTTTCGCGGAAANGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  2:105804/101‑1 (MQ=255)
                                                ctacacTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATg                                                 <  2:3092395/99‑1 (MQ=255)
                                                  acacTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCTACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCg                                               <  1:304244/101‑1 (MQ=255)
                                                  acacTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATgag                                               >  1:3092595/1‑99 (MQ=255)
                                                   cacTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCgg                                              <  2:3336975/101‑1 (MQ=255)
                                                     cTTTCGCGGAAAAGCTGGCGGCGGCGGTGGGCAAGCTCAATGTCGGCGACGGGCCGAAGGACGGCGTGACAACCGGGCCCTTGATCTAGGCCGATGCggac                                            >  2:3923314/1‑99 (MQ=255)
                                                      tttCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCg                                           <  2:811581/101‑1 (MQ=255)
                                                        tCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCgag                                         <  2:3896479/101‑1 (MQ=255)
                                                        tCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCgag                                         <  1:2593038/101‑1 (MQ=255)
                                                        tCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCgag                                         <  1:4486068/101‑1 (MQ=255)
                                                          gcgGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGaa                                       <  2:502593/101‑1 (MQ=255)
                                                          gcgGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGaa                                       >  1:2316626/1‑101 (MQ=255)
                                                             gAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGt                                    <  1:3384036/101‑1 (MQ=255)
                                                             gAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGt                                    <  2:2701691/101‑1 (MQ=255)
                                                              aaaaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTc                                   <  1:1893734/101‑1 (MQ=255)
                                                              aaaaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTc                                   >  1:3547683/1‑101 (MQ=255)
                                                               aaaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCg                                  >  1:294857/1‑101 (MQ=255)
                                                               aaaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCg                                  <  2:2020535/101‑1 (MQ=255)
                                                                aaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGa                                 <  2:254711/101‑1 (MQ=255)
                                                                aaGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGa                                 <  1:3581610/101‑1 (MQ=255)
                                                                   cTGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGa                              >  1:2381028/1‑101 (MQ=255)
                                                                    tGGCGGCGGCGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGaa                             >  2:4670763/1‑101 (MQ=255)
                                                                     ggcggcggcGGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAAc                            >  1:2519949/1‑101 (MQ=255)
                                                                      gcggcggcgGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACa                           <  1:4671019/101‑1 (MQ=255)
                                                                      gcggcggcgGTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACa                           >  1:2651621/1‑101 (MQ=255)
                                                                       cggcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACat                          <  2:4297673/101‑1 (MQ=255)
                                                                         gcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACatat                        <  1:4405267/101‑1 (MQ=255)
                                                                         gcggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACatat                        <  1:952296/101‑1 (MQ=255)
                                                                         gcggcggTGCGCAAGCTCAATGTCGGCGACGGGCGGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACatat                        <  1:904267/101‑1 (MQ=255)
                                                                          cggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATc                       <  1:327004/101‑1 (MQ=255)
                                                                          cggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATc                       >  2:1600405/1‑101 (MQ=255)
                                                                           ggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCg                      <  1:3163470/101‑1 (MQ=255)
                                                                           ggcggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCg                      >  2:1624300/1‑101 (MQ=255)
                                                                             cggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGcc                    >  1:2478439/1‑101 (MQ=255)
                                                                              ggTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCg                   >  1:456024/1‑101 (MQ=255)
                                                                               gTGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGa                  >  1:170539/1‑101 (MQ=255)
                                                                                tGCGCAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCCAt                 >  2:768565/1‑101 (MQ=255)
                                                                                 gcgcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtg                <  2:2735142/101‑1 (MQ=255)
                                                                                 gcgcAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGAtg                >  1:2874656/1‑101 (MQ=255)
                                                                                    cAAGCTGAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtgg             <  1:768639/101‑1 (MQ=255)
                                                                                    cAAGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtgg             >  2:2384719/1‑101 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            >  2:4342505/1‑101 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            >  1:2089227/1‑101 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggt            >  1:1499129/1‑101 (MQ=255)
                                                                                     aaGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggg            >  1:4107395/1‑100 (MQ=255)
                                                                                      aGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggtg           >  2:4255687/1‑101 (MQ=255)
                                                                                      aGCTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATgtggtg           >  1:2616329/1‑101 (MQ=255)
                                                                                       gCTCAATGTCGGCGGCGGGCTGAAGGACGGCGTGACCCCCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGtggtgg          <  2:4052721/101‑1 (MQ=255)
                                                                                        cTCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggc         <  1:1233147/101‑1 (MQ=255)
                                                                                         tCAATGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcg        >  2:3815879/1‑101 (MQ=255)
                                                                                           aaTGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTggcggg      <  2:294823/101‑1 (MQ=255)
                                                                                             tGTCGGCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGCCGAGAAGGACGAGGAACACATCGCCGATGTGGTggcggggg    >  1:2152094/1‑99 (MQ=255)
                                                                                              gtcggcGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGgcgggggg   >  1:4257188/1‑101 (MQ=255)
                                                                                               tcggcgACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGgcggggggg  >  1:1351855/1‑100 (MQ=255)
                                                                                                  |                                                                                                 
CCAGACCTGCGTCTGCGCGAACCGGATCTATGTGCAGGCGGGCGTCTATGACACTTTCGCGGAAAAGCTGGCGGCGGCGGTGCGCAAGCTCAATGTCGTCGACGGGCTGAAGGACGGCGTGACCACCGGGCCCTTGATCTCGGCCGATGCGGTCGAGAAGGTCGAGGAACATATCGCCGATGTGGTGGCGGGCGGC  >  NC_014034/441770‑441965

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG