Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 444,483 C→G 100% L51L (CTG→CTC)  RCAP_rcc00416 ← LysR family transcriptional regulator

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034444,4830C→G100.0% 685.0 184L51L (CTG→CTC) RCAP_rcc00416LysR family transcriptional regulator
Reads supporting (aligned to +/- strand):  new base (80/104):  ref base (0/0):  total (80/104)

Only 100 of 192 total aligned reads displayed.

TCATGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCCAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATGG  >  NC_014034/444385‑444580
                                                                                                  |                                                                                                 
tCATGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAg                                                                                                 <  2:1313463/101‑1 (MQ=255)
 cATGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAgg                                                                                                <  2:1970155/101‑1 (MQ=255)
  aTGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGc                                                                                               >  1:451383/1‑101 (MQ=255)
   tGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCt                                                                                              >  2:2390190/1‑101 (MQ=255)
      aGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGt                                                                                           >  1:2040432/1‑101 (MQ=255)
       ggCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGtt                                                                                          <  2:1757477/101‑1 (MQ=255)
        gCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGttt                                                                                         <  2:2875880/101‑1 (MQ=255)
         ccAAGGATGCTGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTc                                                                                        <  1:102536/101‑1 (MQ=255)
         ccAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTc                                                                                        >  1:103450/1‑101 (MQ=255)
          taaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCtttgggcc                                                                                       <  1:864195/100‑8 (MQ=255)
          cAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTcc                                                                                       >  2:1256166/1‑101 (MQ=255)
           aaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCtttgggcca                                                                                      >  2:864121/1‑93 (MQ=255)
           aaGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCa                                                                                      <  1:1569174/101‑1 (MQ=255)
            aGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCTGTGAATAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAg                                                                                     <  2:4696998/101‑1 (MQ=255)
             ggATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGTGCGAAATAAGATCCACGCCGAGACTGGTTTCCAgc                                                                                    >  2:3847133/1‑101 (MQ=255)
             ggATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAgc                                                                                    >  1:565927/1‑101 (MQ=255)
              gATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGGAGATCCGCGCCGAGGCTGGTTTCCAgcc                                                                                   <  2:3695060/101‑1 (MQ=255)
               aTGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAgccg                                                                                  >  1:2494378/1‑101 (MQ=255)
               aTGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAgccg                                                                                  <  1:318284/101‑1 (MQ=255)
                  cgggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGccgccg                                                                               >  1:1131216/1‑101 (MQ=255)
                   gggcggCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGGTTCCAGCCGCCgg                                                                              >  2:579802/1‑101 (MQ=255)
                    ggcggcGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGa                                                                             <  1:701079/101‑1 (MQ=255)
                     gcggcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGAt                                                                            <  1:624048/101‑1 (MQ=255)
                     gcggcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGAt                                                                            >  2:1873865/1‑101 (MQ=255)
                      cggcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATc                                                                           <  2:2559456/101‑1 (MQ=255)
                        gcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCtt                                                                         <  2:103434/101‑1 (MQ=255)
                        gcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCtt                                                                         >  1:2522327/1‑101 (MQ=255)
                        gcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCtt                                                                         <  2:2993490/101‑1 (MQ=255)
                        gcgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGGTTCCAGCCGCCGGATCtt                                                                         >  2:4088365/1‑101 (MQ=255)
                         cgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTtg                                                                        >  2:1638075/1‑101 (MQ=255)
                         cgTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTtg                                                                        <  1:4379487/101‑1 (MQ=255)
                          gTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTtgt                                                                       >  1:963986/1‑101 (MQ=255)
                          gTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTtgt                                                                       <  2:1371194/101‑1 (MQ=255)
                           tAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTtgtg                                                                      <  2:3735183/101‑1 (MQ=255)
                            aaTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGGCGCCGGATCTTGTgg                                                                     >  1:450243/1‑101 (MQ=255)
                            aaTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTTTgg                                                                     >  1:3459076/1‑101 (MQ=255)
                            aaTCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTgg                                                                     >  1:1370380/1‑101 (MQ=255)
                              tcaggatcccccGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCt                                                                   >  2:3543183/11‑101 (MQ=255)
                              tCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCt                                                                   <  1:3916863/101‑1 (MQ=255)
                              tCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCt                                                                   <  2:3047976/101‑1 (MQ=255)
                               cGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTg                                                                  <  2:4061562/101‑1 (MQ=255)
                                 aGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGAc                                                                >  1:1438111/1‑101 (MQ=255)
                                  gCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACg                                                               <  1:3616060/101‑1 (MQ=255)
                                   cAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACgg                                                              <  1:282581/101‑1 (MQ=255)
                                   cAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCCCCGGATCTTGTGGCTGCCgg                                                              >  1:2843641/1‑101 (MQ=255)
                                     gTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGcc                                                            >  2:3450874/1‑101 (MQ=255)
                                      ttCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCg                                                           <  2:3961046/101‑1 (MQ=255)
                                        ccGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAt                                                         >  1:4485640/1‑101 (MQ=255)
                                         cgcgCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAtt                                                        <  2:3022260/101‑1 (MQ=255)
                                         cgcgCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAtt                                                        <  1:3483499/101‑1 (MQ=255)
                                         cgcgCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAtt                                                        <  2:50450/101‑1 (MQ=255)
                                         cgcgCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGAtt                                                        <  2:2688132/101‑1 (MQ=255)
                                          gcgcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGGTTg                                                       >  1:4179322/1‑101 (MQ=255)
                                          gcgcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTg                                                       <  1:1061151/101‑1 (MQ=255)
                                          gcgcCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTg                                                       <  2:4247546/101‑1 (MQ=255)
                                               cgcgGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCa                                                  <  1:1638175/101‑1 (MQ=255)
                                               cgcgGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCa                                                  <  1:4698757/101‑1 (MQ=255)
                                               cgcgGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCa                                                  <  2:2040311/101‑1 (MQ=255)
                                                    cggTGAACAGCGCCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGccc                                             <  2:1453581/101‑1 (MQ=255)
                                                     ggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCa                                            <  2:1402204/101‑1 (MQ=255)
                                                     ggTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCa                                            <  1:3314028/101‑1 (MQ=255)
                                                      gTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCaa                                           <  2:2090997/101‑1 (MQ=255)
                                                      gTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCaa                                           <  2:606953/101‑1 (MQ=255)
                                                       tGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCaaa                                          <  1:4269895/101‑1 (MQ=255)
                                                        gAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGGCAGGCCCaaaa                                         >  2:938711/1‑100 (MQ=255)
                                                             gCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcg                                    <  1:2995837/101‑1 (MQ=255)
                                                              cGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTcgg                                   <  2:2833270/101‑1 (MQ=255)
                                                                   cgccgGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTTTGGCTGACCGCCGATTGCGTCAGGCCCAAAGCCTTGgcggcg                              >  2:1865053/1‑101 (MQ=255)
                                                                   cgccgGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGCTTGCGGGAGGGCCGAAGCCTCGgcggcg                              >  2:1771226/1‑101 (MQ=255)
                                                                    gccgGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGACCAAAGCCTCCGCGgggc                             >  1:1729132/1‑101 (MQ=255)
                                                                      cgGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCgg                           >  1:1804384/1‑101 (MQ=255)
                                                                      cgGGGCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCgg                           <  2:4142560/101‑1 (MQ=255)
                                                                       ggggCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGGCGATTGGGTCAGGACCAAAGCCTCGGCGGCGCcgg                          >  2:699822/1‑101 (MQ=255)
                                                                       ggggCGGCGCGAAAGAAGATCCGCGGCGAGGCTGGTTGCCAGCCGCCGGGTCTTGTGGCTGACGGCCGATTACTTCAAGCTCCAAGACTTagcggctacgg                          >  2:143832/1‑96 (MQ=255)
                                                                        gggCGGCGCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGt                         <  1:4109262/101‑1 (MQ=255)
                                                                           cggcgCGACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGAc                      <  2:821111/101‑1 (MQ=255)
                                                                                 gACAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGcc                <  1:2244163/101‑1 (MQ=255)
                                                                                  aCAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGGCGGGGCCg               >  1:461336/1‑101 (MQ=255)
                                                                                   cAGAAGATCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGGCGGGGCCgg              >  2:3994471/1‑100 (MQ=255)
                                                                                    agaagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTg             <  1:3089635/101‑1 (MQ=255)
                                                                                    agaagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACAGTGCCGTg             >  2:3155273/1‑101 (MQ=255)
                                                                                    agaagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGTGCCGTg             >  1:1673551/1‑101 (MQ=255)
                                                                                      aagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGGTGACGGACGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGGGGCGTGAt           >  1:3428109/1‑101 (MQ=255)
                                                                                      aagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGGCGGGGCCGTGAt           >  2:3282365/1‑101 (MQ=255)
                                                                                      aagaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGAt           <  1:43254/101‑1 (MQ=255)
                                                                                       agaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATg          <  1:3543402/101‑1 (MQ=255)
                                                                                       agaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGGGCCGTGagg          >  1:3737211/1‑99 (MQ=255)
                                                                                        gaTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGGCCAAAGCCTCGGCCGCGCGGGGGACGCTGCCGCGGTgg         >  2:457191/1‑101 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGTTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATggg        <  1:4630099/101‑1 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATggg        <  2:461292/101‑1 (MQ=255)
                                                                                         aTCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGTGCCGGGATggg        >  2:4338058/1‑101 (MQ=255)
                                                                                          tCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGc       <  1:4501411/101‑1 (MQ=255)
                                                                                          tCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGc       <  1:2390324/101‑1 (MQ=255)
                                                                                          tCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGc       <  1:4750616/101‑1 (MQ=255)
                                                                                          tCCGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGc       <  2:1601092/101‑1 (MQ=255)
                                                                                           ccGGGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCg      <  2:2291240/101‑1 (MQ=255)
                                                                                           ccGCGCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGGGCCGGGATGGGCg      >  1:2075218/1‑101 (MQ=255)
                                                                                            cgcgCCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGGGACGGTGCCGTGATGGGCGa     >  2:4281257/1‑101 (MQ=255)
                                                                                             gcgcCGAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGAt    <  1:1256256/101‑1 (MQ=255)
                                                                                              cgcCGAGGCTGGTTGCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATg   <  2:2731050/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TCATGCAGGCCAAGGATGCGGGCGGCGTAATCGAGCAGTTCCGCGCCCGCGGCGGTGAACAGCGGCCCGCCGGGGCGGCGCGACAGAAGATCCGCGCCCAGGCTGGTTTCCAGCCGCCGGATCTTGTGGCTGACGGCCGATTGCGTCAGGCCCAAAGCCTCGGCGGCGCGGGTGACGGTGCCGTGATGGGCGATGG  >  NC_014034/444385‑444580

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG