BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	530,940	C→G	100%	intergenic (+18/+123)	valS → / ← RCAP_rcc00497	valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	530,940	0	C→G	100.0%	332.8	131	intergenic (+18/+123)	valS/RCAP_rcc00497	valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand): new base (48/79): ref base (1/3): total (49/82)

Only 100 of 141 total aligned reads displayed.


AAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..CCGCGCTTG.TC.GGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530849‑531022


                    gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATAtt                                                        <  2:1579956/101‑1 (MQ=25)


                     aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATAttt                                                       <  1:4277162/101‑1 (MQ=25)


                     aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATAttt                                                       <  2:2292714/101‑1 (MQ=25)


                     aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATAttt                                                       >  2:4599091/1‑101 (MQ=25)


                      agagGCCGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATAtttt                                                      <  1:779504/101‑1 (MQ=21)


                          gcggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGc                                                  <  1:3853696/101‑1 (MQ=25)


                           cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:3140050/101‑1 (MQ=25)


                            ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCaa                                                <  1:1496482/101‑1 (MQ=25)


                            ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCaa                                                <  1:70726/101‑1 (MQ=25)


                            ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCaa                                                <  2:100231/101‑1 (MQ=25)


                            ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCaa                                                <  1:863131/101‑1 (MQ=25)


                             gcgAATGTCGGCACGGGGCTGAAGCTGCTGGCCGAACTCAGATAAAGCAAGAAGGGGTGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAg                                               <  2:4432731/101‑1 (MQ=9)


                             gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAg                                               <  1:1317407/101‑1 (MQ=25)


                             gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAg                                               <  2:1182422/101‑1 (MQ=25)


                             gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..TGCCCCCCG.CCTCCCTCCCTCCGGGATATTTTCGGCAAg                                               >  2:587696/1‑101 (MQ=21)


                              ggAAGAGCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAACGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  1:3994022/100‑1 (MQ=18)


                              cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  1:3739801/101‑1 (MQ=21)


                              cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGa                                              >  2:3249919/1‑101 (MQ=21)


                              cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  2:91611/101‑1 (MQ=21)


                                aaGATCGGCGCGGCGCTGACGCGTCGGGCCGAGCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  1:2685618/101‑1 (MQ=16)


                                aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  2:2637518/1‑101 (MQ=21)


                                aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  2:2377491/101‑1 (MQ=21)


                                aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  1:2832748/101‑1 (MQ=21)


                                 aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           >  1:242824/1‑101 (MQ=21)


                                 aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           <  1:2532523/101‑1 (MQ=21)


                                  gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  2:549339/101‑1 (MQ=21)


                                   aTCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAg                                         <  1:4743727/101‑1 (MQ=21)


                                     cggcgcggcgCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGc                                       <  1:1152109/101‑1 (MQ=21)


                                       gcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGccc                                     <  2:1409247/101‑1 (MQ=21)


                                        cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:4305191/101‑1 (MQ=21)


                                        cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:1525965/101‑1 (MQ=21)


                                        cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:3679670/101‑1 (MQ=21)


                                         gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCgg                                   <  2:1996771/101‑1 (MQ=21)


                                         gcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAAGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGTGATATTTTCGGCAAGATAAAGGACCgg                                   >  2:395583/1‑101 (MQ=19)


                                          cggcgtGGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:3298695/101‑1 (MQ=18)


                                          cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:2861823/101‑1 (MQ=21)


                                          cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  2:552187/101‑1 (MQ=21)


                                          cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:1323845/101‑1 (MQ=21)


                                           ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:1719181/101‑1 (MQ=21)


                                           ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:1908964/101‑1 (MQ=21)


                                           ggcccTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:412470/101‑1 (MQ=21)


                                            gcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAg                                >  1:20581/1‑101 (MQ=21)


                                             cgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGc                               >  1:3600368/1‑101 (MQ=21)


                                             cgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGCCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGCTATTTTCAACAAGATAAAGGCCCGGAAGc                               >  1:686434/1‑101 (MQ=19)


                                              gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  1:723125/1‑101 (MQ=21)


                                              gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:3734980/1‑101 (MQ=21)


                                              gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:2088076/1‑101 (MQ=21)


                                                tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTc                            >  2:2729124/1‑101 (MQ=21)


                                                 gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  2:1286784/101‑1 (MQ=21)


                                                 gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  1:1495817/1‑101 (MQ=21)


                                                 gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  2:3507719/101‑1 (MQ=21)


                                                 gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  1:2804230/1‑101 (MQ=21)


                                                 gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGACCGGAAGCATcc                           >  2:3245601/1‑101 (MQ=18)


                                                  aaGCGTCTGGTCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:49887/1‑101 (MQ=21)


                                                  aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  1:464779/1‑101 (MQ=21)


                                                  aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  1:1603779/101‑1 (MQ=21)


                                                  aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  2:3680943/101‑1 (MQ=21)


                                                   aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  1:765308/101‑1 (MQ=21)


                                                   aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  2:2595886/101‑1 (MQ=21)


                                                     cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  1:3778964/101‑1 (MQ=21)


                                                      gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATATAGGCCCGGAAGCCTCCCTTTa                      <  1:2696145/101‑1 (MQ=21)


                                                      gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      <  2:2271561/101‑1 (MQ=21)


                                                       tCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTAt                     <  2:2806428/101‑1 (MQ=21)


                                                        cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    <  1:3739790/101‑1 (MQ=21)


                                                        cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    <  1:4737451/101‑1 (MQ=21)


                                                         tGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCt                   >  1:4551326/1‑101 (MQ=21)


                                                         tGGCCGAACTCGGATAAAGGAAGAAGGGGGGATCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATcc                   >  1:4213622/1‑100 (MQ=21)


                                                          gtccGAACTCGCATAAAGGAACAAGGGGGGCTC..TTGCCCCCGCTT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAACCCTCCCTTTATCtt                  <  2:830920/99‑1 (MQ=16)


                                                          ggCCGAACTCGGATAAAGGAAGAAGGGGGGGTCT.GCCCCCCGC.TT.CCCTCCCCCCGGGGTTTTTTTGGGAAGATAAAGGACAGGAAGCCTCTCTTTTTCtt                  >  1:3299210/1‑101 (MQ=255)


                                                          ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGACCGGAAGCCTCCTCTTATCtt                  >  1:1852143/1‑101 (MQ=16)


                                                           gCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTg                 >  2:1677188/1‑101 (MQ=21)


                                                             cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               >  1:1024310/1‑101 (MQ=21)


                                                              gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              <  1:2300864/101‑1 (MQ=21)


                                                                acgcGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            <  1:3607280/98‑1 (MQ=21)


                                                                aCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            >  2:2363414/1‑101 (MQ=21)


                                                                aCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGACGGa            >  2:744205/1‑101 (MQ=21)


                                                                 cTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGTCCCGGAAGCCTCCCTTTATCTTGCCGGaa           <  2:1134920/101‑1 (MQ=21)


                                                                 cTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaa           <  2:934099/101‑1 (MQ=21)


                                                                  tCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaaa          >  1:2254242/1‑101 (MQ=21)


                                                                  tCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaaa          <  2:1837758/101‑1 (MQ=21)


                                                                   cGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         <  2:209967/101‑1 (MQ=21)


                                                                   cGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         >  2:337189/1‑101 (MQ=21)


                                                                    ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:1976641/101‑1 (MQ=21)


                                                                    ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:4181980/101‑1 (MQ=21)


                                                                    ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  1:486316/101‑1 (MQ=21)


                                                                    ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:1374166/101‑1 (MQ=21)


                                                                    ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAACATAAAGGCCCCGAAGCCACCCTTTATCTTGCCGGAAata        >  2:3756175/1‑101 (MQ=16)


                                                                     gATAAAGGAAGAGGGGGGGCTCT.CCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:4122/101‑1 (MQ=25)


                                                                     gATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:4422261/101‑1 (MQ=21)


                                                                      aTAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:43528/101‑1 (MQ=21)


                                                                      aTAAAGGAAGAAGGGGGCCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  1:1057524/101‑1 (MQ=21)


                                                                       tAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     >  1:1576063/1‑101 (MQ=21)


                                                                       tAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  2:2732679/101‑1 (MQ=21)


                                                                        aaaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  1:2003620/1‑101 (MQ=21)


                                                                        aaaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  2:2237684/1‑101 (MQ=21)


                                                                         aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  1:4289655/101‑1 (MQ=21)


                                                                         aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:3683174/101‑1 (MQ=21)


                                                                         aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TT.CGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:4448599/101‑1 (MQ=21)


                                                                          aGGAAGAAGGGGGGCTCTGCCCCCCGCT.TC..GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGCAGCCTCACTTTATCTTGACGGAAATATCCCaa  >  1:2013389/1‑101 (MQ=16)


                                                                          aGGAAGAAGGGGGGCTCTGCCCCCCGCT.TC..GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCaa  <  1:4405997/101‑1 (MQ=21)


AAAACCTCGCCGCGCGCGCGGAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..CCGCGCTTG.TC.GGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530849‑531022

Base quality scores:


ATCG

< 3 ≤


ATCG

< 18 ≤


ATCG

< 27 ≤


ATCG

< 33 ≤


ATCG

< 41 ≤


ATCG