Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 530,950 C→T 100% intergenic (+28/+113) valS → / ← RCAP_rcc00497 valyl‑tRNA synthetase/UbiA prenyltransferase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034530,9500C→T100.0% 363.6 136intergenic (+28/+113)valS/RCAP_rcc00497valyl‑tRNA synthetase/UbiA prenyltransferase
Reads supporting (aligned to +/- strand):  new base (48/85):  ref base (1/2):  total (49/87)

Only 100 of 140 total aligned reads displayed.

GAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..CCGCGCTTG.TCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530869‑531022
                                                                                    |                                                                        
gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATAtt                                                        <  2:1579956/101‑1 (MQ=25)
gAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATAtt                                                        >  1:2569929/1‑101 (MQ=25)
 aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATAttt                                                       <  1:4277162/101‑1 (MQ=25)
 aaGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATAttt                                                       >  2:4599091/1‑101 (MQ=25)
  agagGCCGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATAtttt                                                      <  1:779504/101‑1 (MQ=21)
   gagGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTc                                                     <  2:2924684/101‑1 (MQ=25)
   gagGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTc                                                     >  2:711687/1‑101 (MQ=25)
   gagGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTc                                                     <  1:377724/101‑1 (MQ=25)
     ggcggcGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCgg                                                   <  1:587235/101‑1 (MQ=25)
       cggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCa                                                 <  2:3140050/101‑1 (MQ=25)
        ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCaa                                                <  1:863131/101‑1 (MQ=25)
        ggcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCaa                                                <  1:1496482/101‑1 (MQ=25)
         gcgAATGTCGGCACGGGGCTGAAGCTGCTGGCCGAACTCAGATAAAGCAAGAAGGGGTGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAg                                               <  2:4432731/101‑1 (MQ=9)
         gcgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAg                                               <  1:1317407/101‑1 (MQ=25)
          ggAAGAGCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAACGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  1:3994022/100‑1 (MQ=18)
          cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  1:3739801/101‑1 (MQ=21)
          cgAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGa                                              <  2:91611/101‑1 (MQ=21)
            gggATGGGCGCGGCGCTAAAGCGTCTGGCCGAAGTCGGATAAGGGAAAAGGGGGGCCTCT.CCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            <  2:1710019/99‑1 (MQ=16)
            aaGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATa                                            >  2:2637518/1‑101 (MQ=21)
             aGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATaa                                           <  1:2532523/101‑1 (MQ=21)
              gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          <  2:549339/101‑1 (MQ=21)
              gATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATaaa                                          >  2:2082422/1‑101 (MQ=21)
                tCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATGAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAgg                                        <  1:3698639/101‑1 (MQ=21)
                 cggcgcggcgCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGc                                       <  1:1152109/101‑1 (MQ=21)
                  ggcgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGcc                                      >  1:4485568/1‑101 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:4305191/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:3679670/101‑1 (MQ=21)
                    cgcggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCg                                    <  1:1525965/101‑1 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:2861823/101‑1 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  1:1323845/101‑1 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  <  2:552187/101‑1 (MQ=21)
                      cggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGa                                  >  1:425556/1‑101 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:1719181/101‑1 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:1495421/101‑1 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  1:1908964/101‑1 (MQ=21)
                       ggcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:4457543/101‑1 (MQ=21)
                       ggcccTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGaa                                 <  2:412470/101‑1 (MQ=21)
                        gcgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAg                                >  1:20581/1‑101 (MQ=21)
                         cgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGCTATTTTCGGCAAGATCAAGGCCCGGAAGc                               >  1:2703665/1‑101 (MQ=21)
                         cgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGc                               >  1:3600368/1‑101 (MQ=21)
                         cgcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGCCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGCTATTTTCAACAAGATAAAGGCCCGGAAGc                               >  1:686434/1‑101 (MQ=19)
                          gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:3734980/1‑101 (MQ=21)
                          gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  2:2088076/1‑101 (MQ=21)
                          gcTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcc                              >  1:723125/1‑101 (MQ=21)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             >  1:4725210/1‑101 (MQ=21)
                           cTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCt                             <  2:2215165/101‑1 (MQ=21)
                            tGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGcagc                            >  2:3294747/1‑98 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           <  2:1286784/101‑1 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  1:2804230/1‑101 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTcc                           >  1:1495817/1‑101 (MQ=21)
                             gAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGACCGGAAGCATcc                           >  2:3245601/1‑101 (MQ=18)
                              aaGCGTCTGGTCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          >  2:49887/1‑101 (MQ=21)
                              aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  2:3680943/101‑1 (MQ=21)
                              aaGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTccc                          <  1:1603779/101‑1 (MQ=21)
                               aGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  2:2595886/101‑1 (MQ=21)
                               aGCGTCGGCCCGAATTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCt                         <  1:4096693/101‑1 (MQ=16)
                                gCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCtt                        <  1:782722/101‑1 (MQ=21)
                                 cGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCttt                       <  1:3778964/101‑1 (MQ=21)
                                  gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATATAGGCCCGGAAGCCTCCCTTTa                      <  1:2696145/101‑1 (MQ=21)
                                  gTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTa                      <  2:2271561/101‑1 (MQ=21)
                                    cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    <  1:4737451/101‑1 (MQ=21)
                                    cTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATc                    >  1:3362292/1‑101 (MQ=21)
                                     tGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCt                   >  1:4551326/1‑101 (MQ=21)
                                     tGGCCGAACTCGGATAAAGGAAGAAGGGGGGATCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATcc                   >  1:4213622/1‑100 (MQ=21)
                                      gtccGAACTCGCATAAAGGAACAAGGGGGGCTC..TTGCCCCCGCTTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAACCCTCCCTTTATCtt                  <  2:830920/99‑1 (MQ=16)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGGTCT.GCCCCCCGC.TTCCCTCCCCCCGGGGTTTTTTTGGGAAGATAAAGGACAGGAAGCCTCTCTTTTTCtt                  >  1:3299210/1‑101 (MQ=255)
                                      ggCCGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGACCGGAAGCCTCCTCTTATCtt                  >  1:1852143/1‑101 (MQ=16)
                                         cGAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGcc               >  1:1024310/1‑101 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:2543362/1‑101 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:2730375/1‑101 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              <  1:2300864/101‑1 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:1056777/1‑101 (MQ=21)
                                          gAACTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCg              >  2:3538986/1‑101 (MQ=21)
                                           aaCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCgg             >  2:1313813/1‑101 (MQ=21)
                                            aCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGa            >  2:2363414/1‑101 (MQ=21)
                                            aCTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGACGGa            >  2:744205/1‑101 (MQ=21)
                                             cTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGTCCCGGAAGCCTCCCTTTATCTTGCCGGaa           <  2:1134920/101‑1 (MQ=21)
                                             cTCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaa           <  2:934099/101‑1 (MQ=21)
                                              tCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaaa          <  2:1837758/101‑1 (MQ=21)
                                              tCGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGaaa          >  1:2254242/1‑101 (MQ=21)
                                               cGGATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAat         >  2:337189/1‑101 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:4181980/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:4041037/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:1976641/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  2:1374166/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAata        <  1:486316/101‑1 (MQ=21)
                                                ggATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAACATAAAGGCCCCGAAGCCACCCTTTATCTTGCCGGAAata        >  2:3756175/1‑101 (MQ=16)
                                                 gATAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAatat       <  1:4422261/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  2:43528/101‑1 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      >  2:2329835/1‑101 (MQ=21)
                                                  aTAAAGGAAGAAGGGGGCCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATc      <  1:1057524/101‑1 (MQ=21)
                                                   tAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     <  2:2732679/101‑1 (MQ=21)
                                                   tAAAGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATcc     >  1:1576063/1‑101 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  1:2003620/1‑101 (MQ=21)
                                                    aaaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATccc    >  2:2237684/1‑101 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  2:3683174/101‑1 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  1:715327/101‑1 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   <  1:4344193/101‑1 (MQ=21)
                                                     aaGGAAGAAGGGGGGCTCT.GCCCCCCGC.TTCGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCa   >  1:328758/1‑101 (MQ=21)
                                                      aGGAAGAAGGGGGGCTCTGCCCCCCGCT.TC.GCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGCAGCCTCACTTTATCTTGACGGAAATATCCCaa  >  1:2013389/1‑101 (MQ=16)
                                                                                    |                                                                        
GAAGAGGCGGCGAAGATCGGCGCGGCGCTGAAGCGTCTGGCCGAACTCGGATAAAGGAAGAAGGGGGGCTC..CCGCGCTTG.TCGGCTCCCCCCGGGATATTTTCGGCAAGATAAAGGCCCGGAAGCCTCCCTTTATCTTGCCGGAAATATCCCAA  >  NC_014034/530869‑531022

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG