Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 54,149 C→A 100% intergenic (‑11/‑78) regB ← / → senC sensor histidine kinase RegB/electron transport protein SenC

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403454,1490C→A100.0% 1025.4 275intergenic (‑11/‑78)regB/senCsensor histidine kinase RegB/electron transport protein SenC
Reads supporting (aligned to +/- strand):  new base (129/146):  ref base (0/0):  total (129/146)

Only 100 of 304 total aligned reads displayed.

CAATGTCCGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTCCCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAGAC  >  NC_014034/54049‑54246
                                                                                                    |                                                                                                 
  aTGTCCGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGtccctacc                                                                                                 >  1:3857105/1‑101 (MQ=255)
  aTGTCCGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGtccctacc                                                                                                 >  1:4369795/1‑101 (MQ=255)
  aTGTCCGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGtccctacc                                                                                                 <  1:1867408/101‑1 (MQ=255)
      ccGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCt                                                                                             >  1:4521073/1‑101 (MQ=255)
      ccGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCt                                                                                             >  2:2676087/1‑101 (MQ=255)
      ccGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCt                                                                                             >  1:1973946/1‑101 (MQ=255)
        gCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCa                                                                                           <  2:4688033/101‑1 (MQ=255)
         cAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGCCATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCAc                                                                                          <  1:1480970/101‑1 (MQ=255)
         cAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCAc                                                                                          >  1:4026823/1‑101 (MQ=255)
          aGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCAcc                                                                                         <  2:3935781/101‑1 (MQ=255)
          aGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCAcc                                                                                         >  2:191719/1‑101 (MQ=255)
            ccGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCt                                                                                       <  2:4026581/101‑1 (MQ=255)
              ggACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTa                                                                                     >  1:2126761/1‑101 (MQ=255)
              ggACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTa                                                                                     <  2:4739159/101‑1 (MQ=255)
               gACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTAc                                                                                    <  2:2605176/101‑1 (MQ=255)
               gACCCATTCCTCATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCCTAc                                                                                    >  2:3167908/1‑101 (MQ=255)
                aCCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTAcc                                                                                   <  1:1823592/101‑1 (MQ=255)
                 cccATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCg                                                                                  <  2:3057931/101‑1 (MQ=255)
                     ttCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAAc                                                                              >  2:739900/1‑101 (MQ=255)
                      tCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAAcc                                                                             >  1:698618/1‑101 (MQ=255)
                       tcTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAAccc                                                                            <  2:1852910/100‑1 (MQ=255)
                       ccTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAAccc                                                                            >  2:3684868/1‑101 (MQ=255)
                       ccTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAAccc                                                                            >  1:849589/1‑101 (MQ=255)
                        cTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAAcccc                                                                           >  2:2021435/1‑101 (MQ=255)
                         tGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCt                                                                          >  1:80115/1‑101 (MQ=255)
                          gATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCtt                                                                         <  1:969692/101‑1 (MQ=255)
                           aTGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTg                                                                        <  2:2544845/101‑1 (MQ=255)
                           aTGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTg                                                                        >  1:4642857/1‑101 (MQ=255)
                             ggCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGat                                                                      <  1:2587176/101‑1 (MQ=255)
                              gCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGata                                                                     <  2:3592416/101‑1 (MQ=255)
                              gCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGata                                                                     <  2:1261232/101‑1 (MQ=255)
                               gtCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGatat                                                                    <  2:2120037/100‑1 (MQ=255)
                                tCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATAtt                                                                   <  2:230112/101‑1 (MQ=255)
                                 cAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTc                                                                  >  2:771997/1‑101 (MQ=255)
                                  aaaCTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTcc                                                                 <  1:2683209/101‑1 (MQ=255)
                                   aaCTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTccc                                                                <  2:1363073/101‑1 (MQ=255)
                                      ttGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGtt                                                             <  1:4564688/101‑1 (MQ=255)
                                       tGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtg                                                            <  2:2820890/101‑1 (MQ=255)
                                        gCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCCCCCTTACCGCAACCCCTTGATATTCCCGTtgc                                                           <  1:2853079/101‑1 (MQ=255)
                                        gCCGACATGTCGAACTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgc                                                           <  1:4741661/101‑1 (MQ=255)
                                           gACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgcgct                                                        >  2:1695579/1‑101 (MQ=255)
                                           gACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgcgct                                                        >  1:3279183/1‑101 (MQ=255)
                                           gACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgcgct                                                        <  2:4602000/101‑1 (MQ=255)
                                             cATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgcgctgc                                                      <  1:529744/101‑1 (MQ=255)
                                             cATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgcgctgc                                                      >  2:2384317/1‑101 (MQ=255)
                                               tGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTtgcgctgcgc                                                    >  2:3294026/1‑101 (MQ=255)
                                                gTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGca                                                   >  1:1322000/1‑101 (MQ=255)
                                                 tCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaa                                                  >  1:2371499/1‑101 (MQ=255)
                                                 tCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaa                                                  >  1:3067843/1‑101 (MQ=255)
                                                  cGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaat                                                 <  1:3777559/101‑1 (MQ=255)
                                                  cGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaat                                                 <  2:899188/101‑1 (MQ=255)
                                                  cGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaat                                                 <  2:641181/101‑1 (MQ=255)
                                                   gAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCGCCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaatc                                                <  2:3268372/101‑1 (MQ=255)
                                                    aaTTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaatca                                               <  1:1170192/101‑1 (MQ=255)
                                                    aaTTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGcaatca                                               >  2:3455934/1‑101 (MQ=255)
                                                      ttCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATCCCCGTTGCGCTGCGcaatcaat                                             >  1:3669721/1‑101 (MQ=255)
                                                        ccggccggTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAAtct                                           >  2:4095255/1‑101 (MQ=255)
                                                        ccggccggTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAAtct                                           <  2:1158752/101‑1 (MQ=255)
                                                             cggTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGc                                      >  1:164164/1‑101 (MQ=255)
                                                               gTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCAc                                    >  1:3397157/1‑101 (MQ=255)
                                                               gTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCAc                                    <  2:804680/101‑1 (MQ=255)
                                                                tCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACg                                   <  1:2778131/101‑1 (MQ=255)
                                                                tCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACg                                   <  2:1940118/101‑1 (MQ=255)
                                                                 cGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGt                                  >  2:1436695/1‑101 (MQ=255)
                                                                 cGACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGt                                  <  2:4522598/101‑1 (MQ=255)
                                                                  gACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTg                                 <  1:191744/101‑1 (MQ=255)
                                                                  gACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTg                                 >  1:741932/1‑101 (MQ=255)
                                                                  gACAGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTg                                 <  1:1079560/101‑1 (MQ=255)
                                                                     aGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAg                              <  2:3856863/101‑1 (MQ=255)
                                                                     aGCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAg                              <  1:636644/101‑1 (MQ=255)
                                                                      gCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAgg                             >  1:2440554/1‑101 (MQ=255)
                                                                      gCCCTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAgg                             <  1:3795860/101‑1 (MQ=255)
                                                                        ccTCATCATATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGgag                           <  2:929933/101‑1 (MQ=255)
                                                                           catcatATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAgg                        >  2:2638291/1‑101 (MQ=255)
                                                                             tcatATCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCg                      >  2:2135406/1‑101 (MQ=255)
                                                                               atatCTTCCATCGTCGTCCCTACCGCCTCACCCTTACCGCACCCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGTAGAGGCGAt                    <  2:1572333/101‑1 (MQ=255)
                                                                               atatCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGAt                    >  2:293137/1‑101 (MQ=255)
                                                                               atatCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGAt                    >  2:4496547/1‑101 (MQ=255)
                                                                                tatCTGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATg                   <  2:2488175/101‑1 (MQ=255)
                                                                                    tGCCATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACg               >  2:4191925/1‑101 (MQ=255)
                                                                                       cATCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGttt            <  1:4707446/101‑1 (MQ=255)
                                                                                        aTCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTc           <  2:1309915/101‑1 (MQ=255)
                                                                                        aTCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTc           >  1:1507882/1‑101 (MQ=255)
                                                                                        aTCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTc           >  2:3357807/1‑101 (MQ=255)
                                                                                        aTCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTc           >  1:3538733/1‑101 (MQ=255)
                                                                                        aTCGTCGTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTc           <  1:3151351/101‑1 (MQ=255)
                                                                                         tcgtcgTCCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCg          >  1:2318195/1‑101 (MQ=255)
                                                                                           gtcgtcCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAg        >  1:909565/1‑101 (MQ=255)
                                                                                              gtcCCTACCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCaa     >  2:2057440/1‑101 (MQ=255)
                                                                                               tccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    <  1:1230960/101‑1 (MQ=255)
                                                                                               tccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    >  1:226860/1‑101 (MQ=255)
                                                                                               tccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    >  1:2541920/1‑101 (MQ=255)
                                                                                               tccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    <  1:390068/101‑1 (MQ=255)
                                                                                               tccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    >  1:4185018/1‑101 (MQ=255)
                                                                                               tccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    >  2:1058007/1‑101 (MQ=255)
                                                                                               gccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAg    <  2:587842/100‑1 (MQ=255)
                                                                                                ccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAGa   >  1:3191579/1‑101 (MQ=255)
                                                                                                ccctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAGa   >  1:1387081/1‑101 (MQ=255)
                                                                                                 cctaccGCCTCGCCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAGAc  <  2:854640/101‑1 (MQ=255)
                                                                                                 cctaccGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAGAc  >  1:4262407/1‑101 (MQ=255)
                                                                                                    |                                                                                                 
CAATGTCCGCAGCCGGACCCATTCCTGATGGCTCAAACTTGCCGACATGTCGAATTCCGGCCGGTCGACAGCCCTCATCATATCTGCCATCGTCGTCCCTCCCGCCTCACCCTTACCGCAACCCCTTGATATTCCCGTTGCGCTGCGCAATCAATCTCCAGCACGTGAAGGAGAGGCGATGAACGTTTCGAGCAAGAC  >  NC_014034/54049‑54246

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG