Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 566,409 A→G 100% F80L (TTC→CTC)  RCAP_rcc00528 ← carboxymuconolactone decarboxylase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034566,4090A→G100.0% 807.8 216F80L (TTC→CTC) RCAP_rcc00528carboxymuconolactone decarboxylase
Reads supporting (aligned to +/- strand):  new base (104/112):  ref base (0/0):  total (104/112)

Only 100 of 226 total aligned reads displayed.

CTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566311‑566506
                                                                                                  |                                                                                                 
cTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGtt                                                                                                 <  1:2385829/101‑1 (MQ=255)
 tCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTc                                                                                                <  2:2467140/101‑1 (MQ=255)
   ggcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCt                                                                                              <  2:3040783/101‑1 (MQ=255)
    gcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGGGAGTTCCTc                                                                                             >  1:1368474/1‑101 (MQ=255)
    gcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTc                                                                                             <  2:4209952/101‑1 (MQ=255)
    gcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTc                                                                                             <  2:2991548/101‑1 (MQ=255)
    gcggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTc                                                                                             <  1:1951347/101‑1 (MQ=255)
     cggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcg                                                                                            <  1:707687/101‑1 (MQ=255)
     cggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcg                                                                                            <  1:2229043/101‑1 (MQ=255)
      ggcAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgc                                                                                           <  1:3952632/101‑1 (MQ=255)
        cAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTcgcgc                                                                                         <  1:2000728/101‑1 (MQ=255)
           gTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTg                                                                                      <  1:785784/101‑1 (MQ=255)
            ttCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgc                                                                                     >  1:366981/1‑101 (MQ=255)
            ttCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgc                                                                                     <  2:1331413/101‑1 (MQ=255)
              cGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccg                                                                                   <  1:3250175/101‑1 (MQ=255)
               gTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGTGCAGATGTCGGCGAGTTCCTCGCGCGTgccgc                                                                                  >  1:499716/1‑101 (MQ=255)
               gTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTgccgc                                                                                  >  2:4319132/1‑101 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                >  2:2584308/1‑101 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                <  1:1342060/101‑1 (MQ=255)
                 cccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgccc                                                                                >  1:1605052/1‑101 (MQ=255)
                  ccAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccg                                                                               <  1:3896431/101‑1 (MQ=255)
                   cagcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGccgcccgc                                                                              >  1:2391056/1‑101 (MQ=255)
                    agcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcg                                                                             >  1:3137085/1‑101 (MQ=255)
                    agcagGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCcgcg                                                                             >  2:79029/1‑101 (MQ=255)
                         gCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcat                                                                        <  2:2885892/101‑1 (MQ=255)
                          ccAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatc                                                                       <  2:4055205/101‑1 (MQ=255)
                           cAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatca                                                                      <  2:2377325/101‑1 (MQ=255)
                            aaGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGcatcat                                                                     <  1:1212868/101‑1 (MQ=255)
                            aaGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCAAGGCGCAGATGTCGGAGAGTTCCTCGCGCGTGCCGCCCGCGCGcatcat                                                                     >  1:1556442/1‑101 (MQ=255)
                             aGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCatcatc                                                                    <  1:3068311/101‑1 (MQ=255)
                             aGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCatcatc                                                                    <  1:1699559/101‑1 (MQ=255)
                              ggCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcg                                                                   >  1:3567316/1‑101 (MQ=255)
                               gCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgc                                                                  >  2:3247661/1‑101 (MQ=255)
                               gCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgc                                                                  <  1:2888857/101‑1 (MQ=255)
                                cATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGGGAGTTCCTCGCGCGTGCCCCCCGCGCGCATCATcgag                                                                 >  1:3390452/1‑99 (MQ=255)
                                cATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATcgcg                                                                 >  2:827690/1‑101 (MQ=255)
                                    ggCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGa                                                             >  2:695131/1‑101 (MQ=255)
                                      ccGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtg                                                           <  1:3088567/101‑1 (MQ=255)
                                       cccATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  1:1626423/99‑1 (MQ=255)
                                       cGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGAtgt                                                          <  1:1377302/101‑1 (MQ=255)
                                          aTACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaa                                                       >  2:3836288/1‑101 (MQ=255)
                                           tACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGaaa                                                      >  1:1129508/1‑101 (MQ=255)
                                            aCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAg                                                     <  1:1092802/101‑1 (MQ=255)
                                            aCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAg                                                     <  1:3044123/101‑1 (MQ=255)
                                            aCATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACAGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAg                                                     >  2:2323207/1‑101 (MQ=255)
                                              atcatcGCCGGGCCGCCGCCCATCTGGATCGCCCCGGCGCAGATGTCGGAGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCCTCGCGCCGATGTGAAAGcc                                                   >  2:2968903/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                >  2:2159691/1‑101 (MQ=255)
                                                 atcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGAt                                                <  2:4518117/101‑1 (MQ=255)
                                                  tcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATg                                               <  2:3638599/101‑1 (MQ=255)
                                                  tcGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATg                                               <  2:3419417/101‑1 (MQ=255)
                                                   cGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGc                                              >  2:2052332/1‑101 (MQ=255)
                                                    gCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCa                                             >  2:4441546/1‑101 (MQ=255)
                                                        ggCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGc                                         <  1:3690545/101‑1 (MQ=255)
                                                        ggCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGCGc                                         >  2:4004218/1‑101 (MQ=255)
                                                         gccgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCt                                        <  2:4702079/101‑1 (MQ=255)
                                                           cgccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCg                                      <  2:2011537/101‑1 (MQ=255)
                                                            gccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGc                                     <  1:2408223/101‑1 (MQ=255)
                                                            gccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGc                                     >  1:4340332/1‑101 (MQ=255)
                                                             ccgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCa                                    >  1:468914/1‑101 (MQ=255)
                                                              cgccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAg                                   <  2:3686485/101‑1 (MQ=255)
                                                               gccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgc                                  <  2:940491/101‑1 (MQ=255)
                                                               gccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgc                                  <  1:3469639/101‑1 (MQ=255)
                                                               gccCATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgc                                  >  1:2981929/1‑101 (MQ=255)
                                                                cccATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgcg                                 >  1:127038/1‑101 (MQ=255)
                                                                 ccATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAgcgc                                >  1:4033764/1‑101 (MQ=255)
                                                                  cATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCTTGTCACCCGCGCTCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTAGCAGCGCt                               <  2:3944208/101‑1 (MQ=255)
                                                                  cATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCt                               >  1:2123954/1‑101 (MQ=255)
                                                                  cATCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCt                               >  2:1894172/1‑101 (MQ=255)
                                                                    tCTGGATCGCCCCGGCGCAGATGTCGGGGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCGGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgc                             <  2:4627676/101‑1 (MQ=255)
                                                                    tCTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATATGAAAGCCCATGCAGGGCTCGCAGCGCTgc                             <  1:2692888/101‑1 (MQ=255)
                                                                     cTGGATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTgcg                            <  2:3298887/101‑1 (MQ=255)
                                                                       ggATCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGcgcg                          >  1:2599598/1‑101 (MQ=255)
                                                                         aTCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGAt                        >  2:2932535/1‑101 (MQ=255)
                                                                          tCGCCACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATg                       <  1:3350917/101‑1 (MQ=255)
                                                                            gCCACGGCGCAGATGTCNGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGcc                     <  2:1368380/101‑1 (MQ=255)
                                                                             ccACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGccc                    <  2:1556341/101‑1 (MQ=255)
                                                                             ccACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGccc                    >  1:804923/1‑101 (MQ=255)
                                                                              cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   >  2:3733067/1‑101 (MQ=255)
                                                                              cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   >  2:1200358/1‑101 (MQ=255)
                                                                              cACGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCa                   <  2:2530478/101‑1 (MQ=255)
                                                                               aCGGCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCAt                  <  1:2584460/101‑1 (MQ=255)
                                                                                 ggCGCAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATgg                >  2:3354035/1‑101 (MQ=255)
                                                                                  gcgcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGc               <  2:620514/101‑1 (MQ=255)
                                                                                   cgcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGcc              >  2:2713316/1‑101 (MQ=255)
                                                                                   cgcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGcc              <  1:1772132/101‑1 (MQ=255)
                                                                                    gcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCa             <  2:539192/101‑1 (MQ=255)
                                                                                    gcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCa             >  1:4531509/1‑101 (MQ=255)
                                                                                    gcAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCa             <  2:3053671/101‑1 (MQ=255)
                                                                                     cAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAg            <  1:380052/101‑1 (MQ=255)
                                                                                     cAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAg            <  1:3836523/101‑1 (MQ=255)
                                                                                     cAGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAg            >  1:601887/1‑101 (MQ=255)
                                                                                      aGATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgc           <  2:1094484/101‑1 (MQ=255)
                                                                                       gATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGcagatc          >  2:307824/1‑96 (MQ=255)
                                                                                       gATGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcg          >  1:2803619/1‑101 (MQ=255)
                                                                                       gATGGCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcg          <  2:2131677/101‑1 (MQ=255)
                                                                                        aTGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAgcgc         >  2:25968/1‑101 (MQ=255)
                                                                                         tGTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGcgcg        >  1:2641197/1‑101 (MQ=255)
                                                                                          gTCGGCGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGa       >  2:1972299/1‑101 (MQ=255)
                                                                                          gTCGGAGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGa       >  2:1726448/1‑101 (MQ=255)
                                                                                               cGAGTTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAt  >  2:2263167/1‑101 (MQ=255)
                                                                                                  |                                                                                                 
CTCGGCGGCAAGTTCGTCCCAGCAGGCCAAGGCATGGGCCGCATACATCATCGCCGGGCCGCCGCCCATCTGGATCGCCACGGCGCAGATGTCGGCGAATTCCTCGCGCGTGCCGCCCGCGCGCATCATCGCGCCGATGTGAAAGCCGATGCAGGGCTCGCAGCGCTGCGCGATGCCCATGGCCAGCGCGATCCAT  >  NC_014034/566311‑566506

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG