Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 590,814 C→G 100% Q56E (CAA→GAA)  murI → glutamate racemase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034590,8140C→G100.0% 818.8 217Q56E (CAA→GAA) murIglutamate racemase
Reads supporting (aligned to +/- strand):  new base (108/109):  ref base (0/0):  total (108/109)

Only 100 of 227 total aligned reads displayed.

TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911
                                                                                                  |                                                                                                 
tGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGaa                                                                                                 >  2:94118/1‑101 (MQ=255)
 gCCCGGGGTGGCGTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGGGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                <  1:1595902/101‑1 (MQ=255)
 gCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAAc                                                                                                >  2:2068503/1‑101 (MQ=255)
  cccGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACg                                                                                               <  1:1705810/101‑1 (MQ=255)
    cGAGGTGGCCTTTGTCTATTAGGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGc                                                                                             >  1:2695453/1‑101 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            >  2:1866923/1‑101 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            <  2:4375625/101‑1 (MQ=255)
     gAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCt                                                                                            >  1:1811338/1‑101 (MQ=255)
      aGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtg                                                                                           >  2:1664630/1‑101 (MQ=255)
      aGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCtg                                                                                           >  2:2672744/1‑101 (MQ=255)
        gTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGCACGGCtgtg                                                                                         >  2:4586978/1‑101 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTggg                                                                                       >  2:338433/1‑101 (MQ=255)
          ggCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGGggg                                                                                       >  2:1230493/1‑101 (MQ=255)
           gCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGa                                                                                      <  2:4076732/101‑1 (MQ=255)
            ccTTTTTCTATTATGGCGACAATTCCCATGCGCCGTACGGCGCGCGCGCCCCCGACGACATCTACAACCTGTCCTGCCGCGGCGTCGAACGGCTGTGGGAc                                                                                     <  2:571223/101‑1 (MQ=255)
             cTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACg                                                                                    <  2:3861579/101‑1 (MQ=255)
              tttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGa                                                                                   >  2:2413758/1‑101 (MQ=255)
               ttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAg                                                                                  >  2:3758832/1‑101 (MQ=255)
               ttGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAg                                                                                  >  1:1233485/1‑101 (MQ=255)
                tGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAgg                                                                                 >  1:4435474/1‑101 (MQ=255)
                    tattatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTg                                                                             >  2:3049990/1‑101 (MQ=255)
                    tattatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTg                                                                             >  1:720801/1‑101 (MQ=255)
                     attatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGc                                                                            <  2:402293/101‑1 (MQ=255)
                       tatGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGa                                                                          >  1:1915955/1‑101 (MQ=255)
                        tcggCGACAATGCCCATGCGCCCTACTGCGTTCGCGCCCCCGGCGACATCTACAGCCTGACCTGCCGCGGCGTCGAACGGCTGTGTGACGAGGGCTGCGAt                                                                         <  2:1542615/99‑1 (MQ=255)
                         tGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATc                                                                        <  1:2956770/101‑1 (MQ=255)
                         tGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATc                                                                        >  1:400446/1‑101 (MQ=255)
                            cGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTgg                                                                     >  1:3551575/1‑101 (MQ=255)
                              aCAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGGCGAGGGCTGCGATCTGGTg                                                                   >  1:3066754/1‑101 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGa                                                                  >  2:216499/1‑101 (MQ=255)
                               cAATGCCCATGCGCCCTACGGCGTGCGCAACCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGa                                                                  >  2:1090283/1‑101 (MQ=255)
                                 aTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAtt                                                                >  2:4230393/1‑101 (MQ=255)
                                 aTGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGAtt                                                                >  2:2509977/1‑101 (MQ=255)
                                  tGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               >  1:102877/1‑101 (MQ=255)
                                  tGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               >  1:2730462/1‑101 (MQ=255)
                                  tGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTc                                                               >  1:1943593/1‑101 (MQ=255)
                                   gCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCt                                                              <  1:3149189/101‑1 (MQ=255)
                                    cccATGCCCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTg                                                             <  1:4365239/101‑1 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           <  1:2346526/101‑1 (MQ=255)
                                      cATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGc                                                           >  1:539917/1‑101 (MQ=255)
                                       aTGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGcc                                                          >  2:526321/1‑101 (MQ=255)
                                         gcgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTg                                                        >  1:4656876/1‑101 (MQ=255)
                                          cgcCCTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGc                                                       <  2:2100565/101‑1 (MQ=255)
                                          cgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGc                                                       <  2:1402377/101‑1 (MQ=255)
                                          cgcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCAGCGGCGTCGAACGGCTGTGGGACAAGGGCTGCGATCCGGTGATTCTGGCCGGc                                                       >  2:927655/1‑101 (MQ=255)
                                           gcCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCa                                                      <  2:4388677/101‑1 (MQ=255)
                                           ccccGACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCa                                                      <  2:3711929/100‑1 (MQ=255)
                                             ccTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAac                                                    <  1:2672898/101‑1 (MQ=255)
                                              cTACGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  1:4136510/101‑1 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  1:4570988/101‑1 (MQ=255)
                                              cTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAaca                                                   <  2:1616006/101‑1 (MQ=255)
                                               caCGGCCGGCGCACCCCCGACGACATCTAAACCCCGCCCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAacac                                                  <  1:887273/100‑1 (MQ=255)
                                                aCGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 <  1:1162948/101‑1 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 <  2:1914965/101‑1 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 >  2:3280502/1‑101 (MQ=255)
                                                aCGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACg                                                 <  2:163613/101‑1 (MQ=255)
                                                 cGGCGTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACgg                                                <  2:2792641/101‑1 (MQ=255)
                                                   gCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGcc                                              >  2:2889152/1‑101 (MQ=255)
                                                    cGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCt                                             <  2:1811229/101‑1 (MQ=255)
                                                    cGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCt                                             <  1:3646219/101‑1 (MQ=255)
                                                    cGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCt                                             <  1:4734649/101‑1 (MQ=255)
                                                     gTGCGCCCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTc                                            <  2:2648876/101‑1 (MQ=255)
                                                       gcgcCCCCCCGACGACATCTACAGCCTGACCTGCCGCTGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccg                                          <  1:604416/101‑1 (MQ=255)
                                                         gcccccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        <  1:4497777/98‑1 (MQ=255)
                                                         gcccccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTccgcc                                        <  1:2512157/98‑1 (MQ=255)
                                                           aCCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCcgccgc                                      >  2:2367761/1‑101 (MQ=255)
                                                            cccccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCcgcg                                     <  1:2199381/101‑1 (MQ=255)
                                                              cccGACGACATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCgcggc                                   >  2:433687/1‑101 (MQ=255)
                                                                   cgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGa                              <  1:2537084/101‑1 (MQ=255)
                                                                   cgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGa                              <  2:4681170/101‑1 (MQ=255)
                                                                   cgacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGa                              >  1:3382674/1‑101 (MQ=255)
                                                                    gacATCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGaa                             <  1:2413893/101‑1 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAg                          >  1:3888368/1‑101 (MQ=255)
                                                                       aTCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAg                          >  1:1535976/1‑101 (MQ=255)
                                                                        tCTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAgg                         >  2:3446304/1‑101 (MQ=255)
                                                                         cTACAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGa                        >  1:3760457/1‑101 (MQ=255)
                                                                            cAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGTCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     >  1:944486/1‑101 (MQ=255)
                                                                            cAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     <  2:2556200/101‑1 (MQ=255)
                                                                            cAACCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGc                     >  1:3248328/1‑101 (MQ=255)
                                                                             aaCCTGACCTGCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCa                    >  1:3004559/1‑101 (MQ=255)
                                                                                ctgacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGa                 >  1:2970229/1‑101 (MQ=255)
                                                                                 tgccctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGaa                <  2:2952863/98‑1 (MQ=255)
                                                                                  gacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               >  2:275303/1‑101 (MQ=255)
                                                                                  gacctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAAc               <  2:218269/101‑1 (MQ=255)
                                                                                   ccctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACa              <  2:4435224/100‑1 (MQ=255)
                                                                                   acctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACa              <  1:3050080/101‑1 (MQ=255)
                                                                                    cctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAt             <  2:3561069/101‑1 (MQ=255)
                                                                                    cctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAt             <  1:1271712/101‑1 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            >  1:4684080/1‑101 (MQ=255)
                                                                                     ctgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACAtt            >  1:361393/1‑101 (MQ=255)
                                                                                      tgCCGCGGCGTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTg           <  1:1090369/101‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  1:1411423/101‑1 (MQ=255)
                                                                                           cggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATcc      <  1:2452473/101‑1 (MQ=255)
                                                                                            ggcgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTTCGCCGCGGCGCTGAAAAGGACGCAGGAACATTGGATccc     >  2:785888/1‑101 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   <  1:2125397/101‑1 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   >  1:625257/1‑101 (MQ=255)
                                                                                              cgTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGa   <  2:102861/101‑1 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGaa  <  2:2970041/101‑1 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGaa  <  1:3969248/101‑1 (MQ=255)
                                                                                               gTCGAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGaa  <  1:260705/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
TGCCCGAGGTGGCCTTTGTCTATTATGGCGACAATGCCCATGCGCCCTACGGCGTGCGCACCCCCGACGACATCTACAACCTGACCTGCCGCGGCGTCCAACGGCTGTGGGACGAGGGCTGCGATCTGGTGATTCTGGCCTGCAACACGGCCTCCGCCGCGGCGCTGAAAAGGATGCAGGAACATTGGATCCCGAA  >  NC_014034/590716‑590911

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 28 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG