Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 625,791 G→C 100% R533R (CGG→CGC anfA → nitrogen fixation regulatory protein AnfA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034625,7910G→C100.0% 958.6 256R533R (CGG→CGCanfAnitrogen fixation regulatory protein AnfA
Reads supporting (aligned to +/- strand):  new base (111/145):  ref base (0/0):  total (111/145)

Only 100 of 267 total aligned reads displayed.

CGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625694‑625888
                                                                                                 |                                                                                                 
 ggcccAGGGCAATATCGGCCGGGCGGGCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               <  1:2171968/99‑1 (MQ=255)
 gacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               <  2:2648858/101‑1 (MQ=255)
 gacCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATc                                                                                               <  2:2826486/101‑1 (MQ=255)
  acCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATcg                                                                                              <  1:3445646/101‑1 (MQ=255)
     cAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCgcgc                                                                                           <  2:77272/101‑1 (MQ=255)
       gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCtt                                                                                         <  2:2463507/101‑1 (MQ=255)
       gggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCtt                                                                                         <  1:870731/101‑1 (MQ=255)
        ggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTc                                                                                        >  1:806321/1‑101 (MQ=255)
        ggCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTc                                                                                        <  2:3333266/101‑1 (MQ=255)
         gCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCg                                                                                       >  1:4011683/1‑101 (MQ=255)
           aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGc                                                                                     >  1:2623890/1‑101 (MQ=255)
           aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGc                                                                                     >  1:553622/1‑101 (MQ=255)
           aaTATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGc                                                                                     >  2:2345589/1‑101 (MQ=255)
               tCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGa                                                                                 <  1:3621366/101‑1 (MQ=255)
                cGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGAt                                                                                >  2:531059/1‑101 (MQ=255)
                  gCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCg                                                                              <  1:3518364/101‑1 (MQ=255)
                   ccAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGa                                                                             >  2:180171/1‑101 (MQ=255)
                      ggcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACgg                                                                          <  1:633138/101‑1 (MQ=255)
                      ggcggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACgg                                                                          <  2:3705365/101‑1 (MQ=255)
                      ggccgcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACgg                                                                          <  1:4321112/101‑1 (MQ=255)
                         ggcCGGGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                       <  1:3876666/101‑1 (MQ=255)
                         ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                       <  2:3909199/101‑1 (MQ=255)
                         ggcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCa                                                                       >  1:195350/1‑101 (MQ=255)
                          gcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATTGCGCTTCGGCCTGATCGACGGCCaa                                                                      >  2:3642518/1‑101 (MQ=255)
                          gcCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCaa                                                                      >  1:3204997/1‑101 (MQ=255)
                          gcCGAGCTTTTGCAGGTCTAGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCaa                                                                      <  1:1859852/101‑1 (MQ=255)
                           ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAAt                                                                     <  1:2841223/101‑1 (MQ=255)
                           ccGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAAt                                                                     <  1:2363341/101‑1 (MQ=255)
                                cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                <  2:4659461/101‑1 (MQ=255)
                                cTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCg                                                                >  1:2264082/1‑101 (MQ=255)
                                 ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                               <  1:317473/101‑1 (MQ=255)
                                 ttttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGa                                                               <  1:2085378/101‑1 (MQ=255)
                                  tttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGaa                                                              <  2:441338/101‑1 (MQ=255)
                                   ttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAAt                                                             <  1:3373026/101‑1 (MQ=255)
                                   ttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAAt                                                             >  2:2587790/1‑101 (MQ=255)
                                   ttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAAt                                                             <  1:3712172/101‑1 (MQ=255)
                                   ttGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCCGCCTGATCGACGGACAATGACCGAAt                                                             >  2:4643278/1‑101 (MQ=255)
                                    tGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCTGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATa                                                            <  2:1582828/101‑1 (MQ=255)
                                      cAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATAcc                                                          >  2:1408626/1‑101 (MQ=255)
                                        ggTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCgg                                                        >  1:4443815/1‑101 (MQ=255)
                                         gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                       <  2:1513133/101‑1 (MQ=255)
                                         gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                       >  2:3857122/1‑101 (MQ=255)
                                         gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                       >  1:2813570/1‑101 (MQ=255)
                                         gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                       >  1:1182097/1‑101 (MQ=255)
                                         gTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGc                                                       >  1:690793/1‑101 (MQ=255)
                                            tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGTCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                    <  2:927639/101‑1 (MQ=255)
                                            tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                    >  1:3810025/1‑101 (MQ=255)
                                            tcGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGcccc                                                    <  1:1589176/101‑1 (MQ=255)
                                            tcGCGCCGCGTGCTCGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGACTACCGGcccc                                                    <  1:4100620/101‑1 (MQ=255)
                                             cgcgCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGCCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCg                                                   >  1:3102861/1‑101 (MQ=255)
                                                gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                >  2:500091/1‑101 (MQ=255)
                                                gcCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCtt                                                <  2:1294658/101‑1 (MQ=255)
                                                 ccGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTc                                               >  2:4518080/1‑101 (MQ=255)
                                                  cGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcg                                              >  2:3534490/1‑101 (MQ=255)
                                                    ggTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggc                                            <  2:4382332/101‑1 (MQ=255)
                                                     gTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcg                                           >  1:2142694/1‑101 (MQ=255)
                                                      tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                          >  2:3220143/1‑101 (MQ=255)
                                                      tGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTcggcgg                                          <  2:42710/101‑1 (MQ=255)
                                                       gCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCggg                                         >  1:4487702/1‑101 (MQ=255)
                                                        cTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCgggg                                        >  1:1309363/1‑101 (MQ=255)
                                                         tGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGc                                       >  2:115630/1‑101 (MQ=255)
                                                          gggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGcg                                      >  1:2783435/1‑100 (MQ=255)
                                                           ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTGGGCCTGATCGACGGCTAATGCCCGAGTACCGGCCCCCCGTCGGCGAGGcct                                     >  2:1891362/1‑99 (MQ=255)
                                                           ggCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCtt                                     <  2:2713776/101‑1 (MQ=255)
                                                            gCCGGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCttt                                    <  2:382082/101‑1 (MQ=255)
                                                               tGCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTc                                 <  2:3793783/101‑1 (MQ=255)
                                                               ggCGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTc                                 <  2:2974109/100‑1 (MQ=255)
                                                                 cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGGTTTTTTtt                               >  1:3643191/1‑101 (MQ=255)
                                                                 cGGATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtt                               <  2:4177477/101‑1 (MQ=255)
                                                                   gATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCtttt                             >  2:1982882/1‑100 (MQ=255)
                                                                   gATGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTg                             <  2:3445932/101‑1 (MQ=255)
                                                                    aTGGGGCGGCTCGGCATCGGCCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGc                            <  2:3722513/101‑1 (MQ=255)
                                                                    aTGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGc                            <  2:1985168/101‑1 (MQ=255)
                                                                     tGGGGCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCg                           >  2:4316664/1‑101 (MQ=255)
                                                                      ggggCGGCTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGGTTTTTCTTTGCgg                          >  2:1760315/1‑101 (MQ=255)
                                                                        ggcggcTCGGCATCGGCCCGCACCGCTATCGCTCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCa                        <  2:1243943/101‑1 (MQ=255)
                                                                        ggcggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCa                        <  2:1675789/101‑1 (MQ=255)
                                                                          cggcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGc                      >  2:4684076/1‑101 (MQ=255)
                                                                            gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    <  1:4238566/101‑1 (MQ=255)
                                                                            gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    >  1:1776625/1‑101 (MQ=255)
                                                                            gcTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGccc                    <  1:4665105/101‑1 (MQ=255)
                                                                             cTCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCg                   <  1:1353242/101‑1 (MQ=255)
                                                                              tCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCCGCCCGt                  >  2:2239988/1‑101 (MQ=255)
                                                                              tCGGCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACCGCCAATGACCGAATACCGGGCCATCTTCGGCGGGGCTTTTTCTTTGCGGCAGGCCgc                  >  1:4060245/1‑100 (MQ=255)
                                                                                 tcATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCg               <  2:2141784/100‑1 (MQ=255)
                                                                                 gCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCg               <  1:416504/101‑1 (MQ=255)
                                                                                 gCATCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCg               <  2:3994686/101‑1 (MQ=255)
                                                                                    tCGACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGaa            <  1:4275687/101‑1 (MQ=255)
                                                                                      gACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAAtt          <  1:2431057/101‑1 (MQ=255)
                                                                                      gACCCGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGGTCCGGAtt          >  1:2060995/1‑101 (MQ=255)
                                                                                        cccGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTcc        <  2:4458189/101‑1 (MQ=255)
                                                                                        cccGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTcc        <  2:4052713/101‑1 (MQ=255)
                                                                                          cGCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCCGGATTCCGt      >  2:4203680/1‑101 (MQ=255)
                                                                                           gCACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGtt     <  1:3779478/101‑1 (MQ=255)
                                                                                            cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGttt    <  2:2263949/101‑1 (MQ=255)
                                                                                            cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGttt    <  1:1078587/101‑1 (MQ=255)
                                                                                            cACCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCCTTTGGGATTCCCttt    >  1:1027287/1‑101 (MQ=255)
                                                                                             aCCGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTa   >  1:2668184/1‑101 (MQ=255)
                                                                                              ccGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAc  <  1:3045445/101‑1 (MQ=255)
                                                                                              ccGCTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAc  <  1:3257972/101‑1 (MQ=255)
                                                                                                 |                                                                                                 
CGACCCAGGGCAATATCGGCCAGGCGGCCGAGCTTTTGCAGGTCTCGCGCCGGGTGCTGGGCCTGCGGATGGGGCGGCTCGGCATCGACCCGCACCGGTATCGCGCTTCGGCCTGATCGACGGCCAATGACCGAATACCGGCCCCGCTTCGGCGGGGCTTTTTCTTTGCGGCAGCCCGTTCGGAATTCCGTTTAC  >  NC_014034/625694‑625888

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG