Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,521 C→T 2.7% G1696S (GGC→AGC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5210C→T 2.7% 2.9 ‑1.8 ‑1.1145G1696S (GGC→AGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (85/56):  total (89/56)
Fisher's exact test strand distribution p-value = 1.59e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.83e-02

Only 100 of 161 total aligned reads displayed.

CCGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGC  >  NC_014034/678421‑678621
                                                                                                    |                                                                                                    
ccGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGcc                                                                                                      >  2:1772292/1‑101 (MQ=255)
ccGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGcc                                                                                                      <  1:1967313/101‑1 (MQ=255)
 cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                     >  1:739093/1‑101 (MQ=255)
 cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                     >  1:1044640/1‑101 (MQ=255)
 cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                     >  1:1200475/1‑101 (MQ=255)
 cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGAGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                     <  1:987056/101‑1 (MQ=255)
  ggTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCaa                                                                                                    >  1:972970/1‑101 (MQ=255)
  ggTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCaa                                                                                                    >  2:1907390/1‑101 (MQ=255)
    tCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAg                                                                                                  <  2:2402572/101‑1 (MQ=255)
     cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGGTCTGCGCCGTGCCAAAgg                                                                                                 >  1:501098/1‑101 (MQ=255)
     cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGGGCCGTGCCAAAgg                                                                                                 >  1:4085080/1‑101 (MQ=255)
     cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAgg                                                                                                 >  2:455017/1‑101 (MQ=255)
        cgccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccg                                                                                              >  2:617908/1‑101 (MQ=255)
         gccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgc                                                                                             >  2:1988621/1‑101 (MQ=255)
         gccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCCCCGTGCCAAAGgccgc                                                                                             >  2:3528851/1‑101 (MQ=255)
          ccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgcc                                                                                            <  2:3825646/101‑1 (MQ=255)
          ccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgcc                                                                                            <  2:484908/101‑1 (MQ=255)
           cgcgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCa                                                                                           <  2:2702160/101‑1 (MQ=255)
             cgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGc                                                                                         >  2:3952127/1‑101 (MQ=255)
             cgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGc                                                                                         >  2:1976529/1‑101 (MQ=255)
              gACATCGGCGGTTTCCATTCCGCGCAGCGCCCCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCt                                                                                        >  1:4251848/1‑101 (MQ=255)
               aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                       <  1:2501305/101‑1 (MQ=255)
               aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                       >  1:1884773/1‑101 (MQ=255)
                   cggcggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgcc                                                                                   >  2:3277862/1‑101 (MQ=255)
                   cggcggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgcc                                                                                   >  2:137413/1‑101 (MQ=255)
                      cggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgag                                                                                <  1:195306/101‑1 (MQ=255)
                      cggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgag                                                                                >  1:424085/1‑101 (MQ=255)
                       ggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagc                                                                               <  1:1319852/101‑1 (MQ=255)
                       ggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagc                                                                               >  1:2550855/1‑101 (MQ=255)
                       ggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagc                                                                               >  2:4091309/1‑101 (MQ=255)
                        gTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagcc                                                                              <  1:853415/101‑1 (MQ=255)
                         tttCCATTCCGCGCAGCGCCGACGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccg                                                                             >  1:2699374/1‑101 (MQ=255)
                         gttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCGGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccg                                                                             <  2:700006/100‑1 (MQ=255)
                          ttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCGGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccga                                                                            >  1:674405/1‑101 (MQ=255)
                            ccATTCCGCGCCGCGCCGCCGTGGTCAGCGCGGGAATGACCGCGCTGCCCAGCGCCGCAATCTGCGCCCTGCCAAAGGCCGCCAGCTGCCCcgagccgagc                                                                          <  2:1748206/101‑1 (MQ=255)
                            ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                          >  2:2053390/1‑101 (MQ=255)
                            ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                          >  2:106278/1‑101 (MQ=255)
                            ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                          >  1:1674387/1‑101 (MQ=255)
                               ttCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGcgcg                                                                       <  1:1099179/101‑1 (MQ=255)
                                 ccGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGt                                                                     <  1:2421033/101‑1 (MQ=255)
                                     gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                 <  2:972893/101‑1 (MQ=255)
                                     gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                 <  1:3567363/101‑1 (MQ=255)
                                       aGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAccgc                                                               >  1:3232139/1‑101 (MQ=255)
                                           nnnnnnnggTCAGCGCCGGAATGACCGCGCTTCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                           <  1:1560262/94‑1 (MQ=255)
                                           ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                           >  1:209567/1‑101 (MQ=255)
                                           ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                           <  1:2870203/101‑1 (MQ=255)
                                             gccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCt                                                         >  1:3012100/1‑101 (MQ=255)
                                                gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                      >  2:3759489/1‑101 (MQ=255)
                                                gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                      <  2:1086307/101‑1 (MQ=255)
                                                  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                    >  1:1371986/1‑101 (MQ=255)
                                                  ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                    <  1:526122/101‑1 (MQ=255)
                                                        cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                              <  1:1770472/101‑1 (MQ=255)
                                                        cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                              >  2:2463765/1‑101 (MQ=255)
                                                          ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGGGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                            >  1:3302915/1‑101 (MQ=255)
                                                           cGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGa                                           >  2:2798608/1‑101 (MQ=255)
                                                             gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                         >  2:39666/1‑101 (MQ=255)
                                                              aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCCCGACCt                                        >  1:775292/1‑101 (MQ=255)
                                                               aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                       <  2:4084838/101‑1 (MQ=255)
                                                               aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                       >  2:2497404/1‑101 (MQ=255)
                                                               aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                       <  2:1504886/101‑1 (MQ=255)
                                                                tGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgc                                      >  2:4480393/1‑101 (MQ=255)
                                                                 gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                     >  1:1797949/1‑101 (MQ=255)
                                                                 gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                     >  1:454357/1‑101 (MQ=255)
                                                                 gACCGCGCTGCCCAGCGCCGCGATCGGCGCCGTGCCAACGGCCGCCAGCTGCGCCGAGCCGAGCGCGGGGACCGCCGCGCCCGACCGCGCCGCGACCTgcg                                     >  1:3212873/1‑101 (MQ=255)
                                                                  aCCGCCCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                    <  1:4412591/101‑1 (MQ=255)
                                                                       gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                               >  2:3812654/1‑101 (MQ=255)
                                                                       gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGATGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                               >  2:2420716/1‑101 (MQ=255)
                                                                          gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                            >  1:1012659/1‑101 (MQ=255)
                                                                          gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                            <  2:3835857/101‑1 (MQ=255)
                                                                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                          >  2:1401444/1‑101 (MQ=255)
                                                                            ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                          >  2:2565029/1‑101 (MQ=255)
                                                                             cAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcg                         <  2:1674282/101‑1 (MQ=255)
                                                                              aGCGCGGCGATCGGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                        <  1:3811669/101‑1 (MQ=255)
                                                                                cggcgcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactgg                      >  1:2714021/4‑96 (MQ=2)
                                                                                 gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                     <  1:1390997/101‑1 (MQ=255)
                                                                                 gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                     <  1:3573757/101‑1 (MQ=255)
                                                                                    gcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAc                  >  2:1990450/1‑101 (MQ=255)
                                                                                    gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                  >  2:589489/1‑92 (MQ=2)
                                                                                    gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                  >  1:3954396/1‑92 (MQ=2)
                                                                                    gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                  >  2:2905860/1‑92 (MQ=0)
                                                                                      gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                <  2:3302713/101‑1 (MQ=255)
                                                                                      gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                >  2:1288984/1‑101 (MQ=255)
                                                                                       aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACttc               >  2:1138684/1‑100 (MQ=255)
                                                                                       aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg               <  1:106294/101‑1 (MQ=255)
                                                                                         cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc             >  2:4211881/1‑101 (MQ=255)
                                                                                            ggcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg          <  2:1200385/100‑1 (MQ=255)
                                                                                            cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg          <  2:849002/101‑1 (MQ=255)
                                                                                            cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAACCGAGCGCGATGACCGCCGCGCTCGGCAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg          <  1:4714607/101‑1 (MQ=255)
                                                                                               cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg       <  2:37327/101‑1 (MQ=255)
                                                                                               cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg       >  2:1485929/1‑101 (MQ=255)
                                                                                               cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg       >  2:2806232/1‑101 (MQ=255)
                                                                                                gTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcgt      >  1:2651787/1‑101 (MQ=255)
                                                                                                gTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcgt      >  1:1402318/1‑101 (MQ=255)
                                                                                                 tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc     >  2:2714716/1‑101 (MQ=255)
                                                                                                 tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc     >  1:3124276/1‑101 (MQ=255)
                                                                                                 ctccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc     <  1:1706556/99‑1 (MQ=255)
                                                                                                  gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa    <  1:2756800/101‑1 (MQ=255)
                                                                                                  gCCAAAGGCCCCTAGCTGCGCCGAGCCGAGCGCGGTGCCCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa    <  1:2266309/101‑1 (MQ=255)
                                                                                                   ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg   >  1:2047146/1‑101 (MQ=255)
                                                                                                   ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg   >  2:1706451/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGC  >  NC_014034/678421‑678621

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG