Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,522 A→C 2.8% F1695L (TTT→AGC RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5220A→C 2.8% 4.4 ‑1.2 ‑1.4142F1695L (TTT→TTGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (84/54):  total (88/54)
Fisher's exact test strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.39e-02

Only 100 of 163 total aligned reads displayed.

CGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCC  >  NC_014034/678422‑678622
                                                                                                    |                                                                                                    
cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                      >  1:1044640/1‑101 (MQ=255)
cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                      >  1:1200475/1‑101 (MQ=255)
cGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGAGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCa                                                                                                      <  1:987056/101‑1 (MQ=255)
 ggTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCaa                                                                                                     >  1:972970/1‑101 (MQ=255)
 ggTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCaa                                                                                                     <  1:2883280/101‑1 (MQ=255)
 ggTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCaa                                                                                                     >  2:1907390/1‑101 (MQ=255)
    cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGGTCTGCGCCGTGCCAAAgg                                                                                                  >  1:501098/1‑101 (MQ=255)
    cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGGGCCGTGCCAAAgg                                                                                                  >  1:4085080/1‑101 (MQ=255)
    cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAgg                                                                                                  >  2:455017/1‑101 (MQ=255)
      gcgcCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgcc                                                                                                >  2:2756636/1‑101 (MQ=255)
       cgccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccg                                                                                               >  2:617908/1‑101 (MQ=255)
        gccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCCCCGTGCCAAAGgccgc                                                                                              >  2:3528851/1‑101 (MQ=255)
         ccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgcc                                                                                             <  2:484908/101‑1 (MQ=255)
         ccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgcc                                                                                             <  2:3825646/101‑1 (MQ=255)
             gACATCGGCGGTTTCCATTCCGCGCAGCGCCCCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCt                                                                                         >  1:4251848/1‑101 (MQ=255)
              aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                        <  1:2501305/101‑1 (MQ=255)
              aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                        >  2:2432033/1‑101 (MQ=255)
              aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                        >  2:2632755/1‑101 (MQ=255)
               cATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTgc                                                                                       <  2:3657094/101‑1 (MQ=255)
               cATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTgc                                                                                       >  2:3794207/1‑101 (MQ=255)
                  cggcggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgcc                                                                                    >  2:137413/1‑101 (MQ=255)
                    gcggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccga                                                                                  >  1:333424/1‑101 (MQ=255)
                      ggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagc                                                                                >  2:4091309/1‑101 (MQ=255)
                      ggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagc                                                                                <  1:1319852/101‑1 (MQ=255)
                       gTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagcc                                                                               <  1:853415/101‑1 (MQ=255)
                        tttCCATTCCGCGCAGCGCCGACGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccg                                                                              >  1:2699374/1‑101 (MQ=255)
                        gttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCGGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccg                                                                              <  2:700006/100‑1 (MQ=255)
                         ttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCGGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccga                                                                             >  1:674405/1‑101 (MQ=255)
                         ttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccga                                                                             >  1:1569692/1‑101 (MQ=255)
                           ccATTCCGCGCCGCGCCGCCGTGGTCAGCGCGGGAATGACCGCGCTGCCCAGCGCCGCAATCTGCGCCCTGCCAAAGGCCGCCAGCTGCCCcgagccgagc                                                                           <  2:1748206/101‑1 (MQ=255)
                           ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                           >  2:106278/1‑101 (MQ=255)
                           ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                           <  1:263714/101‑1 (MQ=255)
                           ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                           >  1:1674387/1‑101 (MQ=255)
                            cATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAgcg                                                                          <  1:4420072/101‑1 (MQ=255)
                              ttCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGcgcg                                                                        <  1:1099179/101‑1 (MQ=255)
                                  gcgcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGa                                                                    <  2:2550703/101‑1 (MQ=255)
                                    gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                  <  2:972893/101‑1 (MQ=255)
                                      aGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAccgc                                                                >  1:3232139/1‑101 (MQ=255)
                                          nnnnnnnggTCAGCGCCGGAATGACCGCGCTTCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                            <  1:1560262/94‑1 (MQ=255)
                                          ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                            >  1:209567/1‑101 (MQ=255)
                                          ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                            <  1:2870203/101‑1 (MQ=255)
                                           cgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCgcgc                                                           >  2:3275589/1‑101 (MQ=255)
                                            cccgTGGTCGGCGCCGGAATGGCCGCGCTGCCCAGTGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCt                                                          <  2:1901971/100‑1 (MQ=255)
                                              cgTGGTCAGCGCCGGAATGACCGCGCTGCCCCGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCg                                                        >  1:4216623/1‑101 (MQ=255)
                                              cgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCg                                                        <  1:1767852/101‑1 (MQ=255)
                                               gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                       <  2:1086307/101‑1 (MQ=255)
                                               gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                       >  2:3759489/1‑101 (MQ=255)
                                                tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                      <  2:1978170/101‑1 (MQ=255)
                                                 ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                     <  1:526122/101‑1 (MQ=255)
                                                 ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                     >  1:1371986/1‑101 (MQ=255)
                                                         ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGGGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                             >  1:3302915/1‑101 (MQ=255)
                                                           ggAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAc                                           >  1:2411956/1‑101 (MQ=255)
                                                            gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                          >  2:39666/1‑101 (MQ=255)
                                                             aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                         >  2:2267297/1‑101 (MQ=255)
                                                             aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                         <  1:2548400/101‑1 (MQ=255)
                                                             aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCCCGACCt                                         >  1:775292/1‑101 (MQ=255)
                                                              aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                        >  2:2497404/1‑101 (MQ=255)
                                                              aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                        <  2:1504886/101‑1 (MQ=255)
                                                              aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                        <  2:4084838/101‑1 (MQ=255)
                                                                gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                      >  1:1797949/1‑101 (MQ=255)
                                                                 aCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                     >  1:3182212/1‑101 (MQ=255)
                                                                 aCCGCCCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                     <  1:4412591/101‑1 (MQ=255)
                                                                  cagagcTACCCACCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGcc                                    <  2:388071/97‑1 (MQ=255)
                                                                      gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGATGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                >  2:2420716/1‑101 (MQ=255)
                                                                       cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                               >  2:4221149/1‑101 (MQ=255)
                                                                         gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                             <  2:3835857/101‑1 (MQ=255)
                                                                         gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                             >  2:1209747/1‑101 (MQ=255)
                                                                         gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                             >  1:1012659/1‑101 (MQ=255)
                                                                           ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                           >  2:1401444/1‑101 (MQ=255)
                                                                           ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                           <  1:1416792/101‑1 (MQ=255)
                                                                            cAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcg                          <  2:1674282/101‑1 (MQ=255)
                                                                             aGCGCGGCGATCGGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                         <  1:3811669/101‑1 (MQ=255)
                                                                                gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                      <  1:1390997/101‑1 (MQ=255)
                                                                                gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                      <  1:3573757/101‑1 (MQ=255)
                                                                                 ccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCcgcg                     >  2:2020778/1‑101 (MQ=255)
                                                                                   gcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAc                   >  2:1990450/1‑101 (MQ=255)
                                                                                   gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                   >  2:589489/1‑92 (MQ=2)
                                                                                   gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                   >  2:2905860/1‑92 (MQ=0)
                                                                                   gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                   >  1:3954396/1‑92 (MQ=2)
                                                                                     gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                 <  2:3302713/101‑1 (MQ=255)
                                                                                     gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                 >  2:1288984/1‑101 (MQ=255)
                                                                                      aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACttc                >  2:1138684/1‑100 (MQ=255)
                                                                                      aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                <  1:106294/101‑1 (MQ=255)
                                                                                        cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc              >  2:4211881/1‑101 (MQ=255)
                                                                                         tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg             >  1:1942760/1‑101 (MQ=255)
                                                                                           cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg           <  2:849002/101‑1 (MQ=255)
                                                                                           cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAACCGAGCGCGATGACCGCCGCGCTCGGCAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg           <  1:4714607/101‑1 (MQ=255)
                                                                                            gcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgt          <  1:1883959/101‑1 (MQ=255)
                                                                                             ccGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtc         <  1:1976646/101‑1 (MQ=255)
                                                                                              cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGTCGcgtcg        >  1:2265091/1‑101 (MQ=255)
                                                                                              cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg        >  2:1485929/1‑101 (MQ=255)
                                                                                              cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg        >  2:2806232/1‑101 (MQ=255)
                                                                                                tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc      >  2:2714716/1‑101 (MQ=255)
                                                                                                tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc      >  1:3124276/1‑101 (MQ=255)
                                                                                                 gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa     <  1:2756800/101‑1 (MQ=255)
                                                                                                  ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg    >  2:1706451/1‑101 (MQ=255)
                                                                                                  ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg    >  1:2047146/1‑101 (MQ=255)
                                                                                                   cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCTGCGACTTGCCGCGTCGTCAGc   <  1:2702788/101‑1 (MQ=255)
                                                                                                   cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc   >  1:725727/1‑101 (MQ=255)
                                                                                                    aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc  >  2:1343996/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CGGTCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCC  >  NC_014034/678422‑678622

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG