Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,525 G→C 2.8% A1694A (GCC→GCG RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5250G→C 2.8% 2.6 ‑1.2 ‑1.0141A1694A (GCC→GCGRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/0):  ref base (83/54):  total (87/54)
Fisher's exact test strand distribution p-value = 2.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.05e-01

Only 100 of 160 total aligned reads displayed.

TCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCC  >  NC_014034/678425‑678624
                                                                                                    |                                                                                                   
 cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGGGCCGTGCCAAAgg                                                                                                    >  1:4085080/1‑101 (MQ=255)
 cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAgg                                                                                                    >  1:486578/1‑101 (MQ=255)
 cAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAgg                                                                                                    >  2:455017/1‑101 (MQ=255)
    cgccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccg                                                                                                 >  2:617908/1‑101 (MQ=255)
     gccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgc                                                                                                >  2:1988621/1‑101 (MQ=255)
     gccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCCCCGTGCCAAAGgccgc                                                                                                >  2:3528851/1‑101 (MQ=255)
      ccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgcc                                                                                               <  2:484908/101‑1 (MQ=255)
      ccgcGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGgccgcc                                                                                               <  2:3825646/101‑1 (MQ=255)
        gcgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAg                                                                                             >  1:1145088/1‑101 (MQ=255)
         cgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGc                                                                                            >  2:1976529/1‑101 (MQ=255)
         cgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGc                                                                                            >  1:1372513/1‑101 (MQ=255)
         cgACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGc                                                                                            >  2:3952127/1‑101 (MQ=255)
          gACATCGGCGGTTTCCATTCCGCGCAGCGCCCCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCt                                                                                           >  1:4251848/1‑101 (MQ=255)
           aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                          >  2:2632755/1‑101 (MQ=255)
           aCATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTg                                                                                          <  1:2501305/101‑1 (MQ=255)
            cATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTgc                                                                                         >  2:3794207/1‑101 (MQ=255)
            cATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTgc                                                                                         <  2:3657094/101‑1 (MQ=255)
                  cggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgag                                                                                   >  1:424085/1‑101 (MQ=255)
                   ggTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagc                                                                                  <  1:1319852/101‑1 (MQ=255)
                    gTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagcc                                                                                 <  1:853415/101‑1 (MQ=255)
                    gTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagcc                                                                                 >  2:596060/1‑101 (MQ=255)
                     gttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCGGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccg                                                                                <  2:700006/100‑1 (MQ=255)
                      ttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCGGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccga                                                                               >  1:674405/1‑101 (MQ=255)
                      ttCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCgccgagccga                                                                               >  1:1569692/1‑101 (MQ=255)
                        ccATTCCGCGCCGCGCCGCCGTGGTCAGCGCGGGAATGACCGCGCTGCCCAGCGCCGCAATCTGCGCCCTGCCAAAGGCCGCCAGCTGCCCcgagccgagc                                                                             <  2:1748206/101‑1 (MQ=255)
                        ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                             >  2:106278/1‑101 (MQ=255)
                        ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                             <  1:263714/101‑1 (MQ=255)
                        ccATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCcgagccgagc                                                                             >  2:2053390/1‑101 (MQ=255)
                         cATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAgcg                                                                            <  1:4420072/101‑1 (MQ=255)
                           ttCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGcgcg                                                                          <  2:176883/101‑1 (MQ=255)
                           ttCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGcgcg                                                                          <  1:1099179/101‑1 (MQ=255)
                                 gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                    >  1:1045867/1‑101 (MQ=255)
                                 gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                    <  1:3567363/101‑1 (MQ=255)
                                 gcAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAcc                                                                    <  2:972893/101‑1 (MQ=255)
                                     cgccgcCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGAccgccg                                                                >  2:412371/1‑101 (MQ=255)
                                       nnnnnnnggTCAGCGCCGGAATGACCGCGCTTCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                              <  1:1560262/94‑1 (MQ=255)
                                       ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                              <  2:1135437/101‑1 (MQ=255)
                                       ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                              >  1:209567/1‑101 (MQ=255)
                                        cgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCgcgc                                                             >  2:3275589/1‑101 (MQ=255)
                                         gccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCt                                                            >  1:3012100/1‑101 (MQ=255)
                                           cgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCg                                                          <  1:1767852/101‑1 (MQ=255)
                                            gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                         >  2:3759489/1‑101 (MQ=255)
                                             tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                        <  1:3269424/101‑1 (MQ=255)
                                              ggTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACa                                                       <  1:526122/101‑1 (MQ=255)
                                                    cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                 >  2:2463765/1‑101 (MQ=255)
                                                    cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                 <  2:38307/101‑1 (MQ=255)
                                                      ccGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGGGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCcgcg                                               >  1:3302915/1‑101 (MQ=255)
                                                       tggAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGa                                              >  1:983369/2‑101 (MQ=255)
                                                       cGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGa                                              >  2:2798608/1‑101 (MQ=255)
                                                        ggAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAc                                             >  1:2411956/1‑101 (MQ=255)
                                                         gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                            >  2:39666/1‑101 (MQ=255)
                                                          aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                           >  2:2267297/1‑101 (MQ=255)
                                                          aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCt                                           <  1:2548400/101‑1 (MQ=255)
                                                          aaTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCCCGACCt                                           >  1:775292/1‑101 (MQ=255)
                                                           aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                          <  2:4084838/101‑1 (MQ=255)
                                                           aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                          >  2:2497404/1‑101 (MQ=255)
                                                            tGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgc                                         >  2:4480393/1‑101 (MQ=255)
                                                             gACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcg                                        >  1:1797949/1‑101 (MQ=255)
                                                             gACCGCGCTGCCCAGCGCCGCGATCGGCGCCGTGCCAACGGCCGCCAGCTGCGCCGAGCCGAGCGCGGGGACCGCCGCGCCCGACCGCGCCGCGACCTgcg                                        >  1:3212873/1‑101 (MQ=255)
                                                                   gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGATGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                  >  2:2420716/1‑101 (MQ=255)
                                                                     tGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTc                                >  1:2868614/1‑101 (MQ=255)
                                                                      gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                               <  2:3835857/101‑1 (MQ=255)
                                                                      gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                               >  1:1012659/1‑101 (MQ=255)
                                                                      gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                               >  2:1209747/1‑101 (MQ=255)
                                                                        ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                             <  1:1416792/101‑1 (MQ=255)
                                                                        ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                             >  2:2565029/1‑101 (MQ=255)
                                                                        ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                             >  2:1401444/1‑101 (MQ=255)
                                                                         cAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcg                            <  2:1674282/101‑1 (MQ=255)
                                                                          aGCGCGGCGATCGGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                           <  1:3811669/101‑1 (MQ=255)
                                                                             gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                        <  1:1390997/101‑1 (MQ=255)
                                                                             gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                        <  1:3573757/101‑1 (MQ=255)
                                                                                gcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAc                     >  2:1990450/1‑101 (MQ=255)
                                                                                  gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                   <  2:3302713/101‑1 (MQ=255)
                                                                                  gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                   >  2:1288984/1‑101 (MQ=255)
                                                                                   aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACttc                  >  2:1138684/1‑100 (MQ=255)
                                                                                     cTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGcc                >  2:4211881/1‑101 (MQ=255)
                                                                                      tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg               >  1:1942760/1‑101 (MQ=255)
                                                                                        cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg             <  2:849002/101‑1 (MQ=255)
                                                                                        cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAACCGAGCGCGATGACCGCCGCGCTCGGCAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg             <  1:4714607/101‑1 (MQ=255)
                                                                                         gcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgt            <  1:1883959/101‑1 (MQ=255)
                                                                                          ccGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtc           <  1:1976646/101‑1 (MQ=255)
                                                                                          ccGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtc           >  2:156346/1‑101 (MQ=255)
                                                                                           cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGTCGcgtcg          >  1:2265091/1‑101 (MQ=255)
                                                                                           cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg          >  2:2806232/1‑101 (MQ=255)
                                                                                           cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg          >  2:1485929/1‑101 (MQ=255)
                                                                                            gTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcgt         >  1:2651787/1‑101 (MQ=255)
                                                                                             tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc        >  2:2714716/1‑101 (MQ=255)
                                                                                             tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc        >  1:3124276/1‑101 (MQ=255)
                                                                                             ctccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc        <  1:1706556/99‑1 (MQ=255)
                                                                                              gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa       <  1:2756800/101‑1 (MQ=255)
                                                                                              gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCCCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa       <  1:3993649/101‑1 (MQ=255)
                                                                                               ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg      >  1:2047146/1‑101 (MQ=255)
                                                                                               ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg      >  2:1679108/1‑101 (MQ=255)
                                                                                                cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCTGCGACTTGCCGCGTCGTCAGc     <  1:2702788/101‑1 (MQ=255)
                                                                                                cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc     >  1:725727/1‑101 (MQ=255)
                                                                                                 aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc    >  2:1343996/1‑101 (MQ=255)
                                                                                                 aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc    >  2:1393658/1‑101 (MQ=255)
                                                                                                  acggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc   <  2:3021880/99‑1 (MQ=255)
                                                                                                  aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc   >  2:1403453/1‑101 (MQ=255)
                                                                                                   aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc  <  1:617972/101‑1 (MQ=255)
                                                                                                    |                                                                                                   
TCAGCGCCGCGACATCGGCGGTTTCCATTCCGCGCAGCGCCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCC  >  NC_014034/678425‑678624

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG