Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 678,564 G→A 3.3% S1681S (AGC→AGT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034678,5640G→A 3.3% 5.1 ‑1.0 ‑0.0151S1681S (AGC→AGT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (4/1):  ref base (78/67):  total (82/69)
Fisher's exact test strand distribution p-value = 3.78e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.02e-01

Only 100 of 166 total aligned reads displayed.

CCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGG  >  NC_014034/678464‑678664
                                                                                                    |                                                                                                    
ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                                                                      <  1:2870203/101‑1 (MQ=255)
ccgccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCcgcg                                                                                                      >  1:209567/1‑101 (MQ=255)
  gccgTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCt                                                                                                    >  2:3993407/1‑101 (MQ=255)
  cccgTGGTCGGCGCCGGAATGGCCGCGCTGCCCAGTGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCt                                                                                                    <  2:1901971/100‑1 (MQ=255)
     gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                                 >  2:3759489/1‑101 (MQ=255)
     gTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGa                                                                                                 <  2:1086307/101‑1 (MQ=255)
      tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                <  2:906031/101‑1 (MQ=255)
      tGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGAc                                                                                                <  1:3269424/101‑1 (MQ=255)
             cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                                                         <  1:1770472/101‑1 (MQ=255)
             cgcCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGcgccg                                                                                         >  2:2463765/1‑101 (MQ=255)
                 ggAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAc                                                                                     >  1:2411956/1‑101 (MQ=255)
                  gAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGAcc                                                                                    >  2:39666/1‑101 (MQ=255)
                    aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                                  <  2:1504886/101‑1 (MQ=255)
                    aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                                  <  2:4084838/101‑1 (MQ=255)
                    aTGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTg                                                                                  >  2:2497404/1‑101 (MQ=255)
                     tGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgc                                                                                 >  2:4480393/1‑101 (MQ=255)
                       aCCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                                                               >  1:3182212/1‑101 (MQ=255)
                       aCCGCCCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTgcgc                                                                               <  1:4412591/101‑1 (MQ=255)
                            gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                                          >  2:3812654/1‑101 (MQ=255)
                            gcTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGATGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGc                                                                          >  2:2420716/1‑101 (MQ=255)
                             cTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCt                                                                         >  2:4221149/1‑101 (MQ=255)
                               gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                       <  2:3835857/101‑1 (MQ=255)
                               gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                       >  2:1209747/1‑101 (MQ=255)
                               gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                       <  1:3622925/101‑1 (MQ=255)
                               gCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCa                                                                       >  1:1012659/1‑101 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                     >  2:1401444/1‑101 (MQ=255)
                                 ccAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgc                                                                     <  1:1416792/101‑1 (MQ=255)
                                  cAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcg                                                                    <  2:1674282/101‑1 (MQ=255)
                                   aGCGCGGCGATCGGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAgcgc                                                                   <  1:3811669/101‑1 (MQ=255)
                                      gccgcGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGcgccgc                                                                <  1:1390997/101‑1 (MQ=255)
                                       ccgcGATCTGCGCCGCCCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcc                                                               <  1:629253/101‑8 (MQ=2)
                                         gcgATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGAc                                                             >  2:1990450/1‑101 (MQ=255)
                                         gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                                                             >  1:3954396/1‑92 (MQ=2)
                                         gcgATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggccag                                                             >  2:589489/1‑92 (MQ=2)
                                           gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                                                           >  2:1288984/1‑101 (MQ=255)
                                           gATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACtt                                                           <  2:3302713/101‑1 (MQ=255)
                                            aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACttc                                                          >  2:1138684/1‑100 (MQ=255)
                                            aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                          >  1:775329/1‑101 (MQ=255)
                                            aTCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTg                                                          >  1:1315185/1‑101 (MQ=255)
                                               tGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcg                                                       >  1:1942760/1‑101 (MQ=255)
                                                 cgcCGTGCCAAAGGCCGCCAGCTGCGCCGAACCGAGCGCGATGACCGCCGCGCTCGGCAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCcgcg                                                     <  1:4714607/101‑1 (MQ=255)
                                                  gcCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgt                                                    <  1:1883959/101‑1 (MQ=255)
                                                   ccGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtc                                                   >  2:156346/1‑101 (MQ=255)
                                                    cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGTCGcgtcg                                                  >  1:2265091/1‑101 (MQ=255)
                                                    cGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGcgtcg                                                  >  2:2806232/1‑101 (MQ=255)
                                                      tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                                >  1:3124276/1‑101 (MQ=255)
                                                      tGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCgtcgtc                                                >  2:2714716/1‑101 (MQ=255)
                                                       gcaaaaGTCCGCAAGCTGCGCCGAGCCGAGCTCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                               <  1:1562186/98‑1 (MQ=38)
                                                       gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                               <  1:2756800/101‑1 (MQ=255)
                                                       gCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                               <  2:1044561/101‑1 (MQ=255)
                                                       gCCAAAGGCCCCTAGCTGCGCCGAGCCGAGCGCGGTGCCCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCa                                               <  1:2266309/101‑1 (MQ=255)
                                                        ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                              >  2:1706451/1‑101 (MQ=255)
                                                        ccAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAg                                              >  1:2047146/1‑101 (MQ=255)
                                                         cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCTGCGACTTGCCGCGTCGTCAGc                                             <  1:2702788/101‑1 (MQ=255)
                                                         cAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGc                                             >  1:725727/1‑101 (MQ=255)
                                                          aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                            <  1:2053511/101‑1 (MQ=255)
                                                          aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                            >  2:1343996/1‑101 (MQ=255)
                                                          aaaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcc                                            >  2:1393658/1‑101 (MQ=255)
                                                           acggCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                           <  2:3021880/99‑1 (MQ=255)
                                                           aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                           >  2:3830097/1‑101 (MQ=255)
                                                           aaGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGccc                                           >  2:1403453/1‑101 (MQ=255)
                                                            aGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGcccc                                          <  1:617972/101‑1 (MQ=255)
                                                               cctccAGCTGCGCCGATCCGAGCGCGGTGTCCGCCGCGCTCGACAGCGCCGCGTCCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCa                                       <  2:775218/98‑1 (MQ=38)
                                                               ccgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCa                                       >  1:2862646/1‑101 (MQ=255)
                                                                cgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCaa                                      <  2:1315093/101‑1 (MQ=255)
                                                                cgccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCaa                                      >  2:1767307/1‑101 (MQ=255)
                                                                  ccAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATc                                    >  2:2705347/1‑101 (MQ=255)
                                                                   cAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcg                                   <  1:1138770/101‑1 (MQ=255)
                                                                   cAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGATCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcg                                   >  2:1102236/1‑101 (MQ=255)
                                                                   agccggggccgagccgaACGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcg                                   <  2:2462463/94‑1 (MQ=255)
                                                                     gCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgcc                                 <  2:4251601/101‑1 (MQ=255)
                                                                      cTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccg                                >  1:3244311/1‑101 (MQ=255)
                                                                      cTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccg                                >  1:2462570/1‑101 (MQ=255)
                                                                      cTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccg                                <  1:1056500/101‑1 (MQ=255)
                                                                       tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                               >  1:2553325/1‑101 (MQ=255)
                                                                       tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATcgccgc                               <  2:2118891/101‑1 (MQ=255)
                                                                       tGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACGTGCCGCGTCGTCAGCCCCGCAATcgccgc                               >  2:798496/1‑101 (MQ=255)
                                                                         cgcCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCgcgc                             <  1:1767416/101‑1 (MQ=255)
                                                                          gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                            >  1:3752573/1‑101 (MQ=255)
                                                                          gccgagccgaGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCt                            >  1:4257299/1‑101 (MQ=255)
                                                                             gagccgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTggc                         >  2:609811/1‑100 (MQ=255)
                                                                                 cgatcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                     <  1:2503790/101‑1 (MQ=255)
                                                                                 cgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                     <  1:3293689/101‑1 (MQ=255)
                                                                                 cgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                     <  2:1942643/101‑1 (MQ=255)
                                                                                 cgagcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGc                     >  1:1459956/1‑101 (MQ=255)
                                                                                   agcGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcg                   <  1:1988738/101‑1 (MQ=255)
                                                                                     cgcgGTACCCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcg                 <  2:3212672/101‑1 (MQ=255)
                                                                                       cgGTGCCCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCcgcgcg               <  1:3529063/101‑1 (MQ=255)
                                                                                        ggTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGa              >  2:1682005/1‑101 (MQ=255)
                                                                                         gTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGACCCGCAATCCGCGCGCTGGGCAGCCGCGCGAt             >  2:3111599/1‑101 (MQ=38)
                                                                                         gTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGGGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCTGCAATCGCCGCGCTGGTCAGCCGCGCGAt             <  2:1145002/101‑1 (MQ=255)
                                                                                           gACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCt           <  1:3952369/101‑1 (MQ=255)
                                                                                             ccgccgCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgc         <  2:2047025/101‑1 (MQ=255)
                                                                                              cgccgcGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgcg        >  2:69359/1‑101 (MQ=255)
                                                                                               gccgcGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgcgc       >  1:405376/1‑101 (MQ=255)
                                                                                               gccgcGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTgcgc       >  1:919687/1‑101 (MQ=255)
                                                                                                  gcgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGt    >  1:1721947/1‑101 (MQ=255)
                                                                                                   cgcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTg   <  1:2632907/101‑1 (MQ=255)
                                                                                                    gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg  >  2:3732214/1‑101 (MQ=255)
                                                                                                    gcTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTgg  >  1:2465741/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCGCCGTGGTCAGCGCCGGAATGACCGCGCTGCCCAGCGCCGCGATCTGCGCCGTGCCAAAGGCCGCCAGCTGCGCCGAGCCGAGCGCGGTGACCGCCGCGCTCGACAGCGCCGCGACCTGCGCCGTGCTCAGCGCCGCGACTTGCCGCGTCGTCAGCCCCGCAATCGCCGCGCTGGTCAGCCGCGCGATCTGCGCCGTGG  >  NC_014034/678464‑678664

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 39 ≤  ATCG