Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	681,149	T→G	7.6%	M820L (ATC→CTC)	RCAP_rcc00630 ←	ice nucleation protein repeat family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	681,149	0	T→G	7.6%	13.2 ‑3.1 ‑0.1	92	M820L (ATC→CTC)	RCAP_rcc00630	ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (7/0):  ref base (51/34):  total (58/34)
Fisher's exact test strand distribution p-value = 4.38e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.36e-01

AGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCA  >  NC_014034/681052‑681249                                                                                                  |                                                                                                     atcTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                                                   >  1:116815/3‑101 (MQ=25) aGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGc                                                                                                   >  1:4696503/1‑101 (MQ=25)    tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                                                >  1:1688116/1‑98 (MQ=25)    tGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgcc                                                                                                >  2:444624/1‑98 (MQ=25)      cgcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccac                                                                                              >  2:2540869/1‑96 (MQ=25)       gcCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccact                                                                                             >  2:785459/1‑95 (MQ=21)          gTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggc                                                                                          >  1:3242365/1‑92 (MQ=21)          gTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGCGcgccactggc                                                                                          >  2:2598976/1‑92 (MQ=21)                                                   ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                                                 >  1:2486485/11‑101 (MQ=37)                                                   ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                                                 >  1:2146744/11‑101 (MQ=37)                                                   ctgcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcg                                                 >  2:4641260/11‑101 (MQ=37)                                                     gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  1:802631/93‑1 (MQ=255)                                                     gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               >  2:1977956/9‑101 (MQ=38)                                                     gcgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtc                                               <  2:1954341/93‑1 (MQ=255)                                                      cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg                                              >  1:3932651/8‑101 (MQ=255)                                                      cgccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcg                                              <  1:2246550/94‑1 (MQ=255)                                                       gccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGcgtcgt                                             <  1:3182633/95‑1 (MQ=255)                                                        ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                                            >  1:1070933/6‑101 (MQ=255)                                                        ccgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTg                                            >  2:1307949/6‑101 (MQ=255)                                                         cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                                           >  2:2242704/5‑101 (MQ=255)                                                         cgtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGc                                           >  1:3478769/5‑101 (MQ=255)                                                          gtcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCt                                          >  1:553973/4‑101 (MQ=255)                                                           tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         <  2:156003/99‑1 (MQ=255)                                                           tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         <  1:3434051/99‑1 (MQ=255)                                                           tcgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTc                                         <  2:253528/99‑1 (MQ=255)                                                            cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGGTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        >  1:3563359/2‑101 (MQ=255)                                                            cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        >  2:1945420/2‑101 (MQ=255)                                                            cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  2:3558699/100‑1 (MQ=255)                                                            cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        >  1:2825868/2‑101 (MQ=255)                                                            cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  2:2367524/100‑1 (MQ=255)                                                            cgTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCa                                        <  2:2400014/100‑1 (MQ=255)                                                             gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       <  1:418201/101‑1 (MQ=255)                                                             gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       >  2:2644186/1‑101 (MQ=255)                                                             gTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAg                                       >  1:1060687/1‑101 (MQ=255)                                                              tCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgc                                      >  2:4276972/1‑101 (MQ=255)                                                               cAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcg                                     >  1:2666550/1‑101 (MQ=255)                                                                aGCGCCCCGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    <  1:553224/101‑1 (MQ=255)                                                                aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    >  2:901210/1‑101 (MQ=255)                                                                aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    >  1:2656921/1‑101 (MQ=255)                                                                aGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAgcgc                                    >  2:3545356/1‑101 (MQ=255)                                                                 gcgcCACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGcgcg                                   <  2:2109044/101‑1 (MQ=255)                                                                  cgccACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCgg                                  <  2:4192949/101‑1 (MQ=255)                                                                  cgccACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCgg                                  >  2:4529699/1‑101 (MQ=255)                                                                   gccacgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGc                                 >  1:401914/4‑101 (MQ=255)                                                                      acgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCAt                              >  2:831800/1‑101 (MQ=255)                                                                       cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc                             <  1:1937385/101‑1 (MQ=255)                                                                       cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc                             <  1:516239/101‑1 (MQ=255)                                                                       cgaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATc                             <  2:3518632/101‑1 (MQ=255)                                                                        gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  2:1871895/1‑101 (MQ=255)                                                                        gaTGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCt                            >  1:2115750/1‑101 (MQ=255)                                                                          tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCTCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          <  1:4690799/101‑1 (MQ=255)                                                                          tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          >  2:463890/1‑101 (MQ=255)                                                                          tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          <  1:3073881/101‑1 (MQ=255)                                                                          tGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgc                          >  2:3983087/1‑101 (MQ=255)                                                                           gTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcg                         >  1:4334794/1‑101 (MQ=255)                                                                            tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc                        <  2:919627/101‑1 (MQ=255)                                                                            tCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTgcgc                        >  1:4348052/1‑101 (MQ=255)                                                                             cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGcc                       >  1:2094605/1‑101 (MQ=255)                                                                             cATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGcc                       >  1:4601181/1‑101 (MQ=255)                                                                              aTCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCg                      <  2:3851175/101‑1 (MQ=255)                                                                              aTCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGATGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCg                      >  2:4004694/1‑101 (MQ=255)                                                                               tCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGa                     >  1:192013/1‑101 (MQ=255)                                                                                cGGTGCCGAGGCCCTTGATCTCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    <  2:4438536/101‑1 (MQ=255)                                                                                cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    <  2:340256/101‑1 (MQ=255)                                                                                cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    >  1:2221930/1‑101 (MQ=255)                                                                                cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    >  2:1303260/1‑101 (MQ=255)                                                                                cGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAg                    <  1:3532939/101‑1 (MQ=255)                                                                                 ggTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAgg                   >  2:4024344/1‑101 (MQ=255)                                                                                 ggTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAgg                   >  1:859658/1‑101 (MQ=255)                                                                                  gTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGt                  <  1:2625590/101‑1 (MQ=255)                                                                                   tGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTc                 <  1:2283721/101‑1 (MQ=255)                                                                                    gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  1:2908344/101‑1 (MQ=255)                                                                                    gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  2:3331056/101‑1 (MQ=255)                                                                                    gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                >  2:4677406/1‑101 (MQ=255)                                                                                    gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  1:2648103/101‑1 (MQ=255)                                                                                    gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCa                <  2:1861228/101‑1 (MQ=255)                                                                                    gCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGCATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGCCa                >  2:1326607/1‑101 (MQ=255)                                                                                     ccGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAg               <  2:423465/101‑1 (MQ=255)                                                                                       gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  1:2053291/1‑101 (MQ=255)                                                                                       gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  2:2623761/1‑101 (MQ=255)                                                                                       gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             <  2:1243574/101‑1 (MQ=255)                                                                                       gAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGcc             >  1:2248605/1‑101 (MQ=255)                                                                                        aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            >  2:2495492/1‑101 (MQ=255)                                                                                        aGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGccc            <  1:4588572/101‑1 (MQ=255)                                                                                          gCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgc          >  2:2548489/1‑101 (MQ=255)                                                                                           cccTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgcg         >  2:4075111/1‑101 (MQ=255)                                                                                           cccTTGACGGCTTTGGCCCTCAGCGCCGTCACGCTGTCCGTATTCAGCGCCGCAAGCTGCGGCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgcg         <  2:475109/101‑1 (MQ=38)                                                                                           cccTTCATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTACTCAGCGCGGCCATCTGCGCCGAGGTCAGCCcgcg         <  2:1571117/101‑1 (MQ=255)                                                                                            ccTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCgcgc        <  2:2526916/101‑1 (MQ=255)                                                                                             cTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCa       >  2:317524/1‑101 (MQ=255)                                                                                              ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAc      >  2:3033099/1‑101 (MQ=255)                                                                                              ttGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCCTGCTCAGCGCGGCCATCTGCGCCGAGGCCAGCCCGCGCAc      >  1:1184783/1‑101 (MQ=255)                                                                                               tGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTTCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAcc     >  1:227801/1‑101 (MQ=255)                                                                                               tGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCAcc     <  1:3545575/101‑1 (MQ=255)                                                                                                 aTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGc   >  2:2064262/1‑101 (MQ=255)                                                                                                 aTCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGc   >  2:149971/1‑101 (MQ=255)                                                                                                  tCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCa  <  1:2619848/101‑1 (MQ=255)                                                                                                  tCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCa  >  1:3812408/1‑101 (MQ=255)                                                                                                  |                                                                                                     AGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCATCTGATCGCCGGTCAGCGCGACGATGTCATCGGTGCCGAGGCCCTTGATCGCATTGGCACTCAGCGCCGTCACGCTGGCCGTATTCAGCGCCGCAAGCTGCGTCGTGCTCAGCGCGGCCATCTGCGCCGAGGTCAGCCCGCGCACCGCA  >  NC_014034/681052‑681249 Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG