Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,459 G→A 20.0% I716I (ATC→ATT)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4590G→A20.0% 98.8 ‑3.5 ‑0.0190I716I (ATC→ATT) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (13/25):  ref base (82/69):  total (95/95)
Fisher's exact test strand distribution p-value = 3.03e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.16e-01

Only 100 of 211 total aligned reads displayed.

CCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCG  >  NC_014034/681359‑681559
                                                                                                    |                                                                                                    
  gcgATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGAt                                                                                                    <  1:2166742/101‑1 (MQ=255)
    gATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCt                                                                                                  >  1:4579442/1‑101 (MQ=255)
    gATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCt                                                                                                  <  2:191988/101‑1 (MQ=255)
     aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                                 <  1:3850803/101‑1 (MQ=255)
     aTCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTg                                                                                                 <  1:699390/101‑1 (MQ=255)
      tCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGc                                                                                                <  2:2932221/101‑1 (MQ=255)
       cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                               >  1:3274409/1‑101 (MQ=255)
       cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                               >  2:509883/1‑101 (MQ=255)
       cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                               >  1:4341432/1‑101 (MQ=255)
       cTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCg                                                                                               >  2:246712/1‑101 (MQ=255)
         gcgcgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTg                                                                                             <  1:4677662/101‑1 (MQ=255)
            cgcCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTg                                                                                          <  1:3359206/101‑1 (MQ=255)
                tGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCa                                                                                      <  2:341932/101‑1 (MQ=255)
                 gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAg                                                                                     <  2:69896/101‑1 (MQ=255)
                 gAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAg                                                                                     >  1:2196410/1‑101 (MQ=255)
                  aaaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgc                                                                                    <  1:1960564/101‑1 (MQ=255)
                  aaaGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgc                                                                                    <  1:4642062/101‑1 (MQ=255)
                    aGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAgcgc                                                                                  >  2:3514845/1‑101 (MQ=255)
                       ccGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGcc                                                                               >  2:3458996/1‑101 (MQ=255)
                          gCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGAt                                                                            <  1:2003158/101‑1 (MQ=255)
                           cgaTCTTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATc                                                                           <  1:1636810/99‑1 (MQ=255)
                            cATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCt                                                                          <  1:4662578/101‑1 (MQ=255)
                             aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                                         <  1:391185/101‑1 (MQ=255)
                             aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                                         >  1:3843414/1‑101 (MQ=255)
                             aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTg                                                                         >  1:1130143/1‑101 (MQ=255)
                             aTCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTAGTCAGCGCGCCGATCTg                                                                         >  2:4265993/1‑101 (MQ=255)
                              tCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGagatcg                                                                        <  2:4024827/101‑6 (MQ=255)
                               cGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTgcg                                                                       >  2:1700033/1‑101 (MQ=255)
                                gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGCCAGCGCGCCGATCTgcgc                                                                      >  1:114340/1‑101 (MQ=255)
                                  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCg                                                                    >  2:304737/1‑101 (MQ=255)
                                  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCg                                                                    >  1:1016974/1‑101 (MQ=255)
                                   gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGa                                                                   >  1:2906909/1‑101 (MQ=255)
                                   gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGa                                                                   >  2:2654058/1‑101 (MQ=255)
                                     cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGc                                                                 >  1:1709621/1‑101 (MQ=255)
                                      gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTTCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                                <  2:2668232/101‑1 (MQ=255)
                                      gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                                >  2:4059230/1‑101 (MQ=255)
                                      gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGcc                                                                >  1:126515/1‑101 (MQ=255)
                                        gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCa                                                              <  1:1746313/101‑1 (MQ=255)
                                          ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGa                                                            <  1:1554873/101‑1 (MQ=255)
                                              cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGAcgcg                                                        >  2:3316963/1‑101 (MQ=255)
                                                cGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgcc                                                      <  1:3605663/101‑1 (MQ=255)
                                                 ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccg                                                     <  2:467954/101‑1 (MQ=255)
                                                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGcgccgc                                                    >  2:1291037/1‑101 (MQ=255)
                                                      gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTgc                                                <  1:901284/101‑1 (MQ=255)
                                                         ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                             <  1:4343181/101‑1 (MQ=255)
                                                          cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                            <  2:1060608/101‑1 (MQ=255)
                                                          cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGACCCCAGACGCGCCGCCTGCGCcg                                            >  2:3100594/1‑101 (MQ=255)
                                                            tCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgcc                                          <  2:4579189/101‑3 (MQ=255)
                                                             cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                         <  1:3983329/101‑1 (MQ=255)
                                                             cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                         >  2:1517549/1‑101 (MQ=255)
                                                             cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                         >  2:4631046/1‑101 (MQ=255)
                                                             cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGCGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                         >  1:3789320/1‑101 (MQ=255)
                                                               cgcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCgtcgtc                                       >  1:2637116/1‑101 (MQ=255)
                                                                gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCCGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                      <  2:3274208/101‑1 (MQ=255)
                                                                gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                      >  1:2616391/1‑101 (MQ=255)
                                                                 gggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAg                                     <  2:3790946/91‑1 (MQ=2)
                                                                  tGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgc                                    >  2:359491/1‑101 (MQ=255)
                                                                   gATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcg                                   >  2:3848803/1‑101 (MQ=255)
                                                                    aTCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcgc                                  >  2:1740547/1‑101 (MQ=255)
                                                                     tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGcc                                 <  1:1369973/101‑1 (MQ=255)
                                                                     tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGACAGCGcc                                 >  2:2570429/1‑101 (MQ=255)
                                                                     gccgccccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                 <  2:2086493/95‑1 (MQ=2)
                                                                      cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                <  2:2906733/101‑1 (MQ=255)
                                                                       cgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCAc                               >  2:1236656/5‑101 (MQ=2)
                                                                        gcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACg                              <  2:631363/98‑1 (MQ=2)
                                                                         caccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGa                             <  2:3302928/99‑1 (MQ=9)
                                                                          gcCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            >  2:4166812/1‑101 (MQ=255)
                                                                          accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            >  1:1243664/2‑101 (MQ=2)
                                                                          accAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGAt                            <  1:984012/100‑1 (MQ=2)
                                                                           ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                           <  2:666787/101‑1 (MQ=255)
                                                                           ccAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATg                           <  1:4414092/101‑1 (MQ=255)
                                                                            cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                          <  2:4452022/101‑1 (MQ=255)
                                                                             aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                         >  1:2873626/1‑101 (MQ=255)
                                                                             aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                         >  1:2691703/1‑101 (MQ=255)
                                                                             aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                         >  2:2433586/1‑101 (MQ=255)
                                                                              gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                        <  2:3946993/101‑1 (MQ=255)
                                                                               aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTAAt                       >  2:1646819/1‑101 (MQ=255)
                                                                                 ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                     >  1:250044/1‑101 (MQ=255)
                                                                                 ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                     >  1:837536/1‑101 (MQ=255)
                                                                                     ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                 <  1:2392327/94‑1 (MQ=9)
                                                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                >  2:4737815/7‑101 (MQ=9)
                                                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                >  1:4741115/7‑101 (MQ=9)
                                                                                      gccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                <  2:2358347/101‑1 (MQ=255)
                                                                                       ccacgcgcgcCGCGATATGCGTGGTCGTCAGCGCTCCAATCTGCGCCGAGCCCGGACCCCCCGCCTGGGCCGTCGTCAGCGCCACGATGTCATCGGTTCCa               <  2:227776/95‑1 (MQ=21)
                                                                                       ccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCa               <  1:2015153/101‑1 (MQ=255)
                                                                                        cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa              >  2:4319929/1‑101 (MQ=255)
                                                                                          ccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgagg            <  1:1175585/99‑5 (MQ=9)
                                                                                          cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg            <  1:4266240/101‑1 (MQ=255)
                                                                                          cGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAg            >  2:2768087/1‑101 (MQ=255)
                                                                                           gAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGc           >  2:4122712/1‑101 (MQ=255)
                                                                                              cgccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct        <  1:648886/101‑3 (MQ=9)
                                                                                               gccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccctt       <  1:2072614/101‑4 (MQ=2)
                                                                                                ccgcAACCTGCGTGCTCGACAGCGCCAGAAGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg      <  1:1694970/101‑5 (MQ=2)
                                                                                                  gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt    >  2:3148797/1‑101 (MQ=255)
                                                                                                  gcgATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt    <  1:2751155/101‑1 (MQ=255)
                                                                                                   cgATCTGCGTGGTGGTCGGCGCGCCGATCTGCCTCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc   <  1:4050631/101‑1 (MQ=25)
                                                                                                   cgATCTGCGTGCGGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATc   <  2:539470/101‑1 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  >  2:1151613/1‑101 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  <  1:2570581/101‑1 (MQ=255)
                                                                                                    gATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg  >  1:1604753/1‑101 (MQ=255)
                                                                                                    |                                                                                                    
CCGCGATCTGCGCGCCTGAAAGCCCGGCCATCGTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCGATCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCG  >  NC_014034/681359‑681559

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 26 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG