Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,491 C→T 22.3% A706T (GCG→ACG)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,4910C→T22.3% 127.3 ‑3.5 0.0210A706T (GCG→ACG) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (16/31):  ref base (86/77):  total (102/108)
Fisher's exact test strand distribution p-value = 3.09e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 226 total aligned reads displayed.

GTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCG  >  NC_014034/681391‑681589
                                                                                                      |                                                                                                  
gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTgcgc                                                                                                    >  1:3258451/1‑101 (MQ=255)
gTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTgcgc                                                                                                    <  1:1640554/101‑1 (MQ=255)
  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCg                                                                                                  >  1:1016974/1‑101 (MQ=255)
  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCg                                                                                                  >  2:304737/1‑101 (MQ=255)
  ggCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGAGCCg                                                                                                  <  2:4017818/101‑1 (MQ=255)
   gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGa                                                                                                 >  1:2906909/1‑101 (MQ=255)
   gCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGa                                                                                                 <  1:3878016/101‑1 (MQ=255)
     cGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGc                                                                                               >  1:2726182/1‑101 (MQ=255)
      gAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGcc                                                                                              >  2:4059230/1‑101 (MQ=255)
        gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCa                                                                                            >  2:1895284/1‑101 (MQ=255)
        gcccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCa                                                                                            <  1:1746313/101‑1 (MQ=255)
         cccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAg                                                                                           >  1:4376953/1‑101 (MQ=255)
          ccagcccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGa                                                                                          <  1:1554873/101‑1 (MQ=255)
              cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGAcgcg                                                                                      >  2:3833035/1‑101 (MQ=255)
              cccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGAcgcg                                                                                      >  2:3316963/1‑101 (MQ=255)
               ccGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACgcgc                                                                                     <  1:3848647/101‑1 (MQ=255)
                 ggAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGcgccg                                                                                   <  2:467954/101‑1 (MQ=255)
                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGcgccgc                                                                                  >  1:1692907/1‑101 (MQ=255)
                  gagaGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGcgccgc                                                                                  >  2:1291037/1‑101 (MQ=255)
                      gcgcCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTgc                                                                              >  1:2628432/1‑101 (MQ=255)
                         ccGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGAGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGcc                                                                           <  2:4558929/101‑1 (MQ=255)
                          cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcg                                                                          <  2:1060608/101‑1 (MQ=255)
                          cGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGACCCCAGACGCGCCGCCTGCGCcg                                                                          >  2:3100594/1‑101 (MQ=255)
                             cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                                                       <  1:3983329/101‑1 (MQ=255)
                             cgcgCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCcgtcg                                                                       >  2:1517549/1‑101 (MQ=255)
                                gcTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCa                                                                    <  2:3293948/101‑1 (MQ=255)
                                  ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                                  <  2:3440122/92‑1 (MQ=2)
                                  ggagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgc                                                                  <  1:4742664/92‑1 (MQ=2)
                                   gagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgcg                                                                 <  2:1264514/93‑1 (MQ=2)
                                   gagccgcaccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAgcg                                                                 <  1:3384721/93‑1 (MQ=2)
                                    aTCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAgcgc                                                                >  2:1740547/1‑101 (MQ=255)
                                     tCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGACAGCGcc                                                               >  2:2570429/1‑101 (MQ=255)
                                     gccgccccAGATCGGCGGTTTCCAGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGcc                                                               <  2:2086493/95‑1 (MQ=2)
                                      cTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCa                                                              <  2:2906733/101‑1 (MQ=255)
                                       tGCGCCAGATCCGCCGCCTCGAGCGCCGCGGTCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                             <  2:4594053/101‑1 (MQ=255)
                                       tGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCAc                                                             >  2:2810807/1‑101 (MQ=255)
                                            cAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGt                                                        >  1:2452113/1‑101 (MQ=255)
                                             aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                       >  2:2433586/1‑101 (MQ=255)
                                             aGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTc                                                       >  1:2691703/1‑101 (MQ=255)
                                              gATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCa                                                      <  1:2654212/101‑1 (MQ=255)
                                               aTCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCAt                                                     <  2:2448470/101‑1 (MQ=255)
                                                 ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                                   >  2:2667852/1‑101 (MQ=255)
                                                 ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                                   >  1:250044/1‑101 (MQ=255)
                                                 ccgccgCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCg                                                   >  1:837536/1‑101 (MQ=255)
                                                  cgccgcCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCgg                                                  >  1:1219475/1‑101 (MQ=255)
                                                   gcggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGt                                                 <  2:3262802/92‑1 (MQ=9)
                                                    cggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                >  2:3532720/9‑101 (MQ=9)
                                                    ccgccTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTg                                                >  2:2750991/1‑101 (MQ=255)
                                                     ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                               <  1:2012253/94‑1 (MQ=9)
                                                     ggtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGc                                               <  1:2090106/94‑1 (MQ=9)
                                                      gtttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGcc                                              >  2:4737815/7‑101 (MQ=9)
                                                        ttccaGCGCCGCAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccga                                            >  2:2309584/5‑99 (MQ=9)
                                                        cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                                            >  2:4319929/1‑101 (MQ=255)
                                                        cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                                            >  1:4282807/1‑101 (MQ=255)
                                                        cTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaa                                            <  2:1709516/101‑1 (MQ=255)
                                                         tCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                           <  2:625910/101‑1 (MQ=255)
                                                         cggatcatggcgATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCaaa                                           >  2:1176306/10‑101 (MQ=255)
                                                           caGCGCCGCAACCTGCGTGCTCGACAGCGC.CAGAA.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                         >  1:954597/2‑96 (MQ=2)
                                                           caGCGCCGCAACCGGCGTGCTAGACAGCGC.CAG.AGGCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGccgaggc                                         <  2:640729/100‑6 (MQ=2)
                                                            aGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGcc                                        <  2:4158643/101‑1 (MQ=255)
                                                             gcgcCGCGATCTGCGTGCTGGTCAGCTCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCcg                                       <  2:3913077/101‑1 (MQ=255)
                                                              cgccgcAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGccct                                      <  1:648886/101‑3 (MQ=9)
                                                                ccgcGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCgg                                    >  1:1709743/1‑101 (MQ=255)
                                                                ccgcAACCTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGcccttg                                    <  1:2197683/101‑5 (MQ=2)
                                                                  gcgATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                  <  1:2751155/101‑1 (MQ=255)
                                                                  gcgATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                  >  2:3148797/1‑101 (MQ=255)
                                                                  gcgATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGAt                                  <  1:1700138/101‑1 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCGAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                <  2:725484/101‑1 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                >  1:1604753/1‑101 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                >  1:1175207/1‑101 (MQ=255)
                                                                    gATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcg                                <  1:2570581/101‑1 (MQ=255)
                                                                     accTGCGTCCTGGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgc                               <  2:4258274/99‑1 (MQ=2)
                                                                      tCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGTGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgcc                              <  1:4081175/101‑1 (MQ=255)
                                                                      ccTGCGTGCTCGACAGCGCCAGA.A.GCTGCGTCGAGCCCAGCGCCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCGAGGCCCTTGATcgca                              >  2:4657948/2‑100 (MQ=2)
                                                                       cTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccg                             <  1:4738116/101‑1 (MQ=255)
                                                                        tGCGTGCTGGTTAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATcgccgc                            <  2:1004462/101‑1 (MQ=255)
                                                                         gCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                           <  1:4122590/101‑1 (MQ=255)
                                                                         gCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCgccgcc                           >  2:225326/1‑101 (MQ=255)
                                                                          cGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGccgccg                          <  1:509934/101‑1 (MQ=255)
                                                                           gTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGt                         <  2:4458266/101‑1 (MQ=255)
                                                                            tGCTGGTCGGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                        <  2:4117500/101‑1 (MQ=255)
                                                                            tGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                        >  2:1655766/1‑101 (MQ=255)
                                                                            tGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                        >  2:235040/1‑101 (MQ=255)
                                                                            tGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTg                        >  1:4438953/1‑101 (MQ=255)
                                                                              cTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGcc                      >  1:1342590/1‑101 (MQ=255)
                                                                               tGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGccc                     <  2:3685157/101‑1 (MQ=255)
                                                                                ggTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                    <  1:2062363/101‑1 (MQ=255)
                                                                                ggTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCa                    >  2:507516/1‑101 (MQ=255)
                                                                                   cAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                 >  1:2605464/1‑101 (MQ=255)
                                                                                   cAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                 <  1:2825582/101‑1 (MQ=255)
                                                                                   cAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAgcg                 >  2:4225365/1‑101 (MQ=255)
                                                                                       gcgcCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGcgccgc             >  1:3812341/1‑101 (MQ=255)
                                                                                           cG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc         <  2:4438703/101‑1 (MQ=255)
                                                                                           cG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCAcc         >  1:1119008/1‑101 (MQ=255)
                                                                                                tCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTc      <  1:694452/101‑1 (MQ=255)
                                                                                                 cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg     <  2:2492160/101‑1 (MQ=255)
                                                                                                 cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg     <  1:3703746/101‑1 (MQ=255)
                                                                                                 cTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCg     <  1:359525/101‑1 (MQ=255)
                                                                                                  tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc    <  1:4254638/101‑1 (MQ=255)
                                                                                                  tGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGc    <  2:3705232/101‑1 (MQ=255)
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GTGGCCGAGCCCAGCCCGGAGAGCGCCGTCGCGCTGATCTGCGCCAGATCCGCCGCCTCGAGCGCCGCGATCTGCGTGCTGGTCAGCGCGCCG.A.TCTGCGCCGAGCCCAGACGCGCCGCCTGCGCCGTCGTCAGCGCCACGATGTCATCGGTGCCAAAGCCGCGGATCGCCGCCGTGCCCAGCGCCGCCACCGTCGCCG  >  NC_014034/681391‑681589

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG