Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 681,689 T→C 9.8% S640G (AGC→GGC)  RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034681,6890T→C 9.8% 37.7 ‑4.4 ‑0.0183S640G (AGC→GGC) RCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (14/4):  ref base (75/90):  total (89/94)
Fisher's exact test strand distribution p-value = 1.20e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.89e-01

Only 100 of 191 total aligned reads displayed.

GAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAGC  >  NC_014034/681589‑681789
                                                                                                    |                                                                                                    
gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                                                      >  1:2358759/1‑101 (MQ=255)
gAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCt                                                                                                      <  2:1249336/101‑1 (MQ=255)
 aaGCCAGCGCCGCGATCTGCGCCGTGCTCATCGCCGCCGCCTGAACCGAGCCAAGCCCGCCCACCGCAAGGCTGGAAAGCGCGCCGACCTGATCGGTGCTc                                                                                                     >  2:4440771/1‑101 (MQ=38)
  ggcaatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                                                    <  2:3242164/95‑1 (MQ=2)
  ggaatccgccccAATCTGCGCCGCGCGCACCGCGGGGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCa                                                                                                    <  1:1915918/95‑1 (MQ=0)
  aGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCa                                                                                                    <  2:4319887/101‑1 (MQ=255)
    ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGc                                                                                                  >  2:669555/1‑101 (MQ=255)
    ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGc                                                                                                  >  2:4416117/1‑101 (MQ=255)
    ccAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGc                                                                                                  <  1:3110301/101‑1 (MQ=255)
    ccAGCGCCGCGAGCTGCGACGTGCTCAGCGCCGCCGCCTGGACCGAGCCAAGTCCGGCCACCGCAAGGCGGGAGAGCGCGCCGACCTGATCGCTGCTCAGc                                                                                                  <  2:1573500/101‑1 (MQ=38)
    caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                                  >  1:2214811/5‑100 (MQ=2)
    caatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAga                                                                                                  >  1:4301093/5‑100 (MQ=2)
     cAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCt                                                                                                 >  2:2708343/1‑101 (MQ=255)
     aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                                                 >  2:132732/4‑99 (MQ=2)
     aatcgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgac                                                                                                 >  1:3791181/4‑99 (MQ=2)
      aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCtt                                                                                                <  2:2736927/101‑1 (MQ=255)
      aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCtt                                                                                                >  2:3879322/1‑101 (MQ=255)
      aGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCtt                                                                                                <  1:1308041/101‑1 (MQ=255)
       gcgcCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTg                                                                                               <  1:2932226/101‑1 (MQ=255)
        cgccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactgc                                                                                              >  2:3966056/1‑96 (MQ=2)
         gccgcGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGcc                                                                                             <  1:2340771/101‑1 (MQ=255)
         gccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggc                                                                                             >  1:3613603/1‑95 (MQ=2)
         gccgcGATCTGCGCCGCGCTCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggc                                                                                             >  1:2077166/1‑95 (MQ=2)
          cggcgATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCg                                                                                            <  2:3524799/99‑1 (MQ=255)
           cgcgATCTGCTCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGa                                                                                           >  1:1539142/1‑101 (MQ=255)
           cgcgACCTGCGCCGCCCCCGCCGCCGCCGGCGGCGGCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAgactggcca                                                                                           <  2:4709455/101‑9 (MQ=255)
             ccaTCTCCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATc                                                                                         <  1:4755058/99‑1 (MQ=255)
              gATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCt                                                                                        <  1:2834840/101‑1 (MQ=255)
                tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                                                      <  1:3046935/101‑1 (MQ=255)
                tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                                                      >  1:4017584/1‑101 (MQ=255)
                tCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGc                                                                                      >  1:3662517/1‑101 (MQ=255)
                  tGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGt                                                                                    >  2:1209287/1‑101 (MQ=255)
                    cgcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcg                                                                                  <  1:3317165/101‑1 (MQ=255)
                     gcCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgc                                                                                 >  1:2870453/1‑101 (MQ=255)
                      ccGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTcgcg                                                                                >  1:2844249/1‑101 (MQ=255)
                          gCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCa                                                                            >  1:2442242/1‑101 (MQ=255)
                          gCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCa                                                                            >  1:694326/1‑101 (MQ=255)
                            tCAGCGCCGCGATCTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCGCGACCTGATCGGTGCCCAGACTGGCCAGCTGCATCGTGCCCAgc                                                                          >  1:2166598/1‑100 (MQ=0)
                            tCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAgg                                                                          >  1:3951620/1‑101 (MQ=255)
                            tCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAgg                                                                          <  1:3515057/101‑1 (MQ=255)
                             cAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGc                                                                         <  1:2493394/101‑1 (MQ=255)
                             cAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGc                                                                         >  2:1482601/1‑101 (MQ=255)
                                cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                                                      <  2:1118922/101‑1 (MQ=255)
                                cgccgccgcCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCg                                                                      >  2:1707426/1‑101 (MQ=255)
                                 gccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGa                                                                     >  2:56429/1‑101 (MQ=255)
                                 gccgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGa                                                                     >  1:2650319/1‑101 (MQ=255)
                                   cgccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGaaa                                                                   >  1:1225266/1‑101 (MQ=255)
                                    gccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                                                  <  2:1175117/101‑1 (MQ=255)
                                    gccgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAc                                                                  >  2:825056/1‑101 (MQ=255)
                                      cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccg                                                                <  2:3229764/101‑1 (MQ=255)
                                      cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccg                                                                <  2:2041376/101‑1 (MQ=255)
                                      cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAAccg                                                                <  1:2959826/101‑1 (MQ=255)
                                      cgccTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGCCCCGAAAccg                                                                >  1:1012196/1‑101 (MQ=255)
                                       atcTGCGCCGAGCCGAGCCCGCCGACCGCGGCACTGGCCAGCGCCCCGACCTGATCGGTGCCCAGACTGGCCAGCTGCATCGTGCCCAGCGCCGTCAccgc                                                               >  2:4458574/3‑101 (MQ=2)
                                          tGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACcgccgc                                                            <  1:4148772/101‑1 (MQ=255)
                                             aCCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCt                                                         >  2:1975384/1‑101 (MQ=255)
                                                gAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGcc                                                      >  1:3449760/1‑101 (MQ=255)
                                                 aGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGccc                                                     >  2:2716696/1‑101 (MQ=255)
                                                  gCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCa                                                    <  1:4375326/101‑1 (MQ=255)
                                                    cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGGTCAGGCCCGAAACCGCAGCGCTGCCCAGc                                                  >  2:805594/1‑101 (MQ=255)
                                                    cAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGc                                                  >  2:1035131/1‑101 (MQ=255)
                                                     aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                                                 <  1:825129/101‑1 (MQ=255)
                                                     aaGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGcc                                                 >  2:1366915/1‑101 (MQ=255)
                                                      aGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGccc                                                >  2:4520553/1‑101 (MQ=255)
                                                        cccGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcg                                              <  2:1011210/101‑1 (MQ=255)
                                                        cccGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcg                                              <  2:4141406/101‑1 (MQ=255)
                                                         ccGGTCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgc                                             >  2:4245685/1‑101 (MQ=255)
                                                          cGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgcg                                            >  1:3980959/1‑101 (MQ=255)
                                                          cGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCcgcg                                            >  1:3850231/1‑101 (MQ=255)
                                                             ccACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATc                                         >  1:4281755/1‑101 (MQ=255)
                                                             ccACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTAAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATc                                         <  1:4706263/101‑1 (MQ=255)
                                                              cACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCt                                        <  1:1593205/101‑1 (MQ=255)
                                                                   cAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGcc                                   <  1:4320176/101‑1 (MQ=255)
                                                                    aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                                  >  2:2529140/1‑101 (MQ=255)
                                                                    aaGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCg                                  >  1:1465590/1‑101 (MQ=255)
                                                                        cTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGcc                              >  2:4043560/1‑101 (MQ=255)
                                                                         tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc                             >  2:2259209/1‑101 (MQ=255)
                                                                         tGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGccc                             >  2:923004/1‑101 (MQ=255)
                                                                          ggAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCa                            >  1:3163253/1‑101 (MQ=255)
                                                                            agagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGc                          >  2:3792521/1‑101 (MQ=255)
                                                                            agagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGc                          >  1:758230/1‑101 (MQ=255)
                                                                             gagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGGTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCg                         >  1:4048018/1‑101 (MQ=255)
                                                                             gagCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCCATCTGCGCCGTCCCCAGCg                         >  1:1002484/1‑101 (MQ=255)
                                                                               gcgcgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTc                       <  1:4543290/101‑1 (MQ=255)
                                                                                cgcgcCGGCCTGATCGGTGCTCAGCTTCCCGATCTGCGTCGCGATCAGGCCCGAAGCCGTCGCGCTGCCCAGCCCCGGGATCTGCGCCGTTCCCAGCGTCg                      <  2:1539041/101‑1 (MQ=255)
                                                                                  cgcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCa                    <  1:3361339/101‑1 (MQ=255)
                                                                                   gcCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCaa                   <  2:458732/101‑1 (MQ=255)
                                                                                    ccGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAg                  <  2:240092/101‑1 (MQ=255)
                                                                                     cGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGc                 <  2:2650165/101‑1 (MQ=255)
                                                                                      gACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCt                <  1:4520806/101‑1 (MQ=255)
                                                                                        ccTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTgc              <  1:173667/101‑1 (MQ=255)
                                                                                         cTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTgcg             >  2:1791628/1‑101 (MQ=255)
                                                                                            aTCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCg          >  2:4720521/1‑101 (MQ=255)
                                                                                              cGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTc        <  1:4487393/101‑1 (MQ=255)
                                                                                               ggTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCg       <  2:2873450/101‑1 (MQ=255)
                                                                                                 tGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGAc     >  1:15604/1‑101 (MQ=255)
                                                                                                 tGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGAc     <  2:3163053/101‑1 (MQ=255)
                                                                                                  gCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACa    <  2:2691549/101‑1 (MQ=255)
                                                                                                   cTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAg   >  2:1049413/1‑101 (MQ=255)
                                                                                                    tCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACTgc  <  1:2716850/101‑1 (MQ=255)
                                                                                                    |                                                                                                    
GAAGCCAGCGCCGCGATCTGCGCCGTGCTCAGCGCCGCCGCCTGCACCGAGCCAAGCCCGGCCACCGCAAGGCTGGAGAGCGCGCCGACCTGATCGGTGCTCAGCTTGCCGATCTGCGTCGCGCTCAGGCCCGAAACCGCCGCGCTGCCCAGCCCCGCGATCTGCGCCGTGCCCAGCGTCGCAAGCTGCGCCGTCGACAGC  >  NC_014034/681589‑681789

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG