Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 682,593 C→G 9.5% E338D (GAG→GAC RCAP_rcc00630 ← ice nucleation protein repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034682,5930C→G 9.5% 39.3 ‑3.6 ‑0.0199E338D (GAG→GACRCAP_rcc00630ice nucleation protein repeat family protein
Reads supporting (aligned to +/- strand):  new base (5/14):  ref base (99/81):  total (104/95)
Fisher's exact test strand distribution p-value = 2.76e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.96e-01

Only 100 of 207 total aligned reads displayed.

TCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTGCGCCG  >  NC_014034/682493‑682693
                                                                                                     |                                                                                                    
tCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTcc                                                                                                      >  2:4503712/1‑101 (MQ=255)
 cTGTCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCt                                                                                                     <  1:3909966/101‑1 (MQ=255)
 cTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCt                                                                                                     >  1:1353116/1‑101 (MQ=255)
  tGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTc                                                                                                    >  2:1927858/1‑101 (MQ=255)
   gCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCg                                                                                                   <  2:4252064/101‑1 (MQ=255)
     cgcgTGCCCAGACCCCCCAGCGGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGt                                                                                                 <  2:2974154/101‑1 (MQ=255)
       cgTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCg                                                                                               >  2:2047793/1‑101 (MQ=255)
         tGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaa                                                                                             >  2:3533789/1‑101 (MQ=255)
          gCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGaaa                                                                                            <  2:718807/101‑1 (MQ=255)
           cccAGATCCCCCAGCTGGGCCGGGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAg                                                                                           <  2:1985583/101‑1 (MQ=255)
               gAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGcccc                                                                                       >  1:1384239/1‑101 (MQ=255)
                cgccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCt                                                                                      >  1:1885415/6‑101 (MQ=37)
                 gccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCtt                                                                                     <  2:2782014/97‑1 (MQ=37)
                  ccgccAGCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCttg                                                                                    <  2:2600036/98‑2 (MQ=37)
                   ccccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                                                                   <  2:1906701/101‑1 (MQ=255)
                   ccccAGCGGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCa                                                                                   <  2:2165414/101‑1 (MQ=255)
                     ccAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGGTCCCGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgc                                                                                 <  1:4060728/101‑1 (MQ=255)
                       aGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAgcgc                                                                               >  1:431124/1‑101 (MQ=255)
                        gCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGcgcg                                                                              <  1:3335553/101‑1 (MQ=255)
                        gCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcg                                                                              <  2:426741/101‑1 (MQ=25)
                        gCTGCGCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcg                                                                              <  2:4218152/101‑1 (MQ=25)
                         cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcgg                                                                             >  1:512498/1‑101 (MQ=255)
                         cTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCACCTTCAGcgcgc                                                                             >  2:1273313/1‑100 (MQ=255)
                          tGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                                                                            >  2:466941/1‑101 (MQ=255)
                          tGGCCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCgcggc                                                                            <  2:4061720/101‑1 (MQ=255)
                            cgCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcgccac                                                                          <  2:244022/100‑5 (MQ=25)
                             gCCGTGCTCAGCGCCGCCACCTGCTGCGAGGTCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcgccact                                                                         <  2:2833706/101‑6 (MQ=21)
                              ccGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTg                                                                        <  1:36113/101‑1 (MQ=255)
                              ccGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTg                                                                        <  1:906199/101‑1 (MQ=255)
                              ccGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTg                                                                        >  1:4587206/1‑101 (MQ=255)
                              ccGTGCTCAGCGCCGCCACCTGCTGCGAGGCCATCGCCGCCA.TCTGATCGCCGGTCAGCGCGACGATGTCGCCGGTCGACAGCCCCTTGAGcgcgccactg                                                                        <  2:3923360/101‑7 (MQ=21)
                               cGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGc                                                                       >  2:4146098/1‑101 (MQ=255)
                                 tGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCg                                                                     <  1:391503/101‑1 (MQ=255)
                                   cTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGAt                                                                   >  1:1049712/1‑101 (MQ=255)
                                   cTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGAt                                                                   >  2:2297673/1‑101 (MQ=255)
                                    tCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATc                                                                  >  2:3146290/1‑101 (MQ=255)
                                     cAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcg                                                                 <  2:3008287/101‑1 (MQ=255)
                                     cAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcg                                                                 <  2:2467621/101‑1 (MQ=255)
                                      aGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgc                                                                >  2:641612/1‑101 (MQ=255)
                                       gcgcCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgcc                                                               <  2:1706223/101‑1 (MQ=255)
                                         gccgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATcgccgc                                                             >  1:1959417/1‑101 (MQ=255)
                                           cgccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaa                                                           >  2:1091461/1‑101 (MQ=255)
                                            gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa                                                          <  1:2592249/101‑1 (MQ=255)
                                            gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa                                                          >  2:3719108/1‑101 (MQ=255)
                                            gccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCaaa                                                          <  1:824250/101‑1 (MQ=255)
                                             ccATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAg                                                         <  2:3472299/101‑1 (MQ=255)
                                              cATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAgg                                                        <  1:2860437/101‑1 (MQ=255)
                                              cATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAgg                                                        >  1:4126742/1‑101 (MQ=255)
                                              cATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAgg                                                        >  2:2909949/1‑101 (MQ=255)
                                                 cTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCcg                                                     <  1:1666574/101‑1 (MQ=255)
                                                     gcCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGcc                                                 <  1:2387627/101‑1 (MQ=255)
                                                     gcCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGcc                                                 <  2:512447/101‑1 (MQ=255)
                                                       cccGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCgg                                               <  2:3477940/101‑1 (MQ=255)
                                                         cGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaa                                             <  1:1591513/101‑1 (MQ=255)
                                                          ggccAGCGCCGCCATTGCGGTCG.TGCTCAGCCCGGCCATGTCCTCGGGCGAAAGCCCCTTGAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaaa                                            <  1:1273404/98‑1 (MQ=255)
                                                           gTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGaaaa                                           <  1:2548057/101‑1 (MQ=255)
                                                            tCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAg                                          <  1:2469584/101‑1 (MQ=255)
                                                            tCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAg                                          >  1:4512441/1‑101 (MQ=255)
                                                             cAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAgg                                         >  1:2503225/1‑101 (MQ=255)
                                                             cAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAgg                                         >  1:3665/1‑101 (MQ=255)
                                                             cAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAgg                                         >  1:3541182/1‑101 (MQ=255)
                                                             cAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAgg                                         >  1:3114468/1‑101 (MQ=255)
                                                                 gccgccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGcc                                     >  1:2794762/1‑101 (MQ=255)
                                                                     ccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATc                                 <  2:1384145/101‑1 (MQ=255)
                                                                     ccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATc                                 >  2:3225121/1‑101 (MQ=255)
                                                                     ccAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATc                                 <  2:4586953/101‑1 (MQ=255)
                                                                      cAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCt                                <  1:74569/101‑1 (MQ=255)
                                                                       aGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTg                               >  1:3869257/1‑101 (MQ=255)
                                                                       aGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATATg                               >  1:4705161/1‑101 (MQ=255)
                                                                        gTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGc                              >  1:1415264/1‑101 (MQ=255)
                                                                         ttCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCt                             >  1:2266128/1‑101 (MQ=255)
                                                                         ttCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCt                             >  2:262629/1‑101 (MQ=255)
                                                                          tCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTc                            <  2:870231/101‑1 (MQ=255)
                                                                          tCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTc                            >  2:2119011/1‑101 (MQ=255)
                                                                           cGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCg                           >  2:410076/1‑101 (MQ=255)
                                                                           cGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCg                           >  1:4580085/1‑101 (MQ=255)
                                                                           cGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCg                           >  2:3847531/1‑101 (MQ=255)
                                                                             aTCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGc                         <  1:2014557/101‑1 (MQ=255)
                                                                               cG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCg                       <  2:2875565/101‑1 (MQ=255)
                                                                               cG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCg                       <  1:2521087/101‑1 (MQ=255)
                                                                               cG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCg                       <  1:2928332/101‑1 (MQ=255)
                                                                                g.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCga                      >  1:4404970/1‑101 (MQ=255)
                                                                                  tGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCgag                     <  1:641676/101‑1 (MQ=255)
                                                                                   gCTCAGCCCGGCCGTGTCCTCGGTCGAAAGCCCCGTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCgaga                    <  1:4078340/101‑1 (MQ=255)
                                                                                   gCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCgaga                    <  2:1019353/101‑1 (MQ=255)
                                                                                     tCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAgc                  >  2:1165860/1‑101 (MQ=255)
                                                                                     tCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAgc                  <  1:1000084/101‑1 (MQ=255)
                                                                                      cAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAgcg                 >  1:1080587/1‑101 (MQ=255)
                                                                                        gCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGcc               >  1:2077272/1‑101 (MQ=255)
                                                                                          ccGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGa             >  2:1449831/1‑101 (MQ=255)
                                                                                           cGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGaa            <  1:3820869/101‑1 (MQ=255)
                                                                                           cGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGaa            <  2:2310216/101‑1 (MQ=255)
                                                                                             gCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAg          >  1:44631/1‑101 (MQ=255)
                                                                                             gCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAg          <  2:3607042/101‑1 (MQ=255)
                                                                                                 tGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTgc      >  2:149266/1‑101 (MQ=255)
                                                                                                  gTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTgcg     >  1:2989035/1‑101 (MQ=255)
                                                                                                  gTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTgcg     <  2:4598147/101‑1 (MQ=255)
                                                                                                   tCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTgcgc    >  1:259481/1‑101 (MQ=255)
                                                                                                    ccTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTGCGcc   >  1:1098221/1‑101 (MQ=255)
                                                                                                     cTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTGCGCCg  <  1:3225322/101‑1 (MQ=255)
                                                                                                     |                                                                                                    
TCTGCCGCGTGCCCAGAACCCCCAGCTGGGCCGTGCTCAGCGCCGCCATCTGCGCCCCGGTCAGCGCCGCCAGTTCGATCG.TGCTCAGCCCGGCCATGTCCTCGGTCGAAAGCCCCTTCAGCGCGGCGGTGCCGATCGCCGCAAAGGCCGCGCCGGAAAAGGCGCCGATCTGCTCGGCCGAGAGCGCCGAAAGCTGCGCCG  >  NC_014034/682493‑682693

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG