Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 774,645 G→C 100% intergenic (‑99/+21) oppB1 ← / ← oppA1 oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034774,6450G→C100.0% 106.9 28intergenic (‑99/+21)oppB1/oppA1oligopeptide ABC transporter permease OppB/oligopeptide ABC transporter periplasmic oligopeptide‑binding protein OppA
Reads supporting (aligned to +/- strand):  new base (27/1):  ref base (0/0):  total (27/1)

CCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.C.GA.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774600‑774730
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gcccggcgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCa                                  >  1:585880/6‑101 (MQ=255)
   cggcgcCCCCCCGCCCGGGTCGGACCGGCCCCGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCCGTCGGCCACGTTCCAGTa                               >  2:4697769/3‑101 (MQ=255)
      cgcCCCCCCGCCCGGGTCGGACCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCCATCACTTCGCCCGCGACCAGTCAGCCACGTTCCAGTATTc                            >  2:4263997/1‑101 (MQ=255)
                  cgggtcggaCCGGCCCTGCCGGTCC.GACCC.TCCGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc                >  1:397252/9‑101 (MQ=255)
                  cgggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGCATTCGCTGTCGCAGGc                >  2:4488293/9‑101 (MQ=255)
                  cgggtcggaCCGGCCCTGCCGGT.CAGA.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGc                >  2:4230560/9‑101 (MQ=255)
                   gggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTCTTCGCTGTCCCAGGCg               >  1:1622236/8‑101 (MQ=255)
                    ggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGt              >  2:975293/7‑101 (MQ=255)
                    ggtcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCAGCCACGTTCCAGTATTCGCTGTCCCAGGCGt              >  2:1279058/7‑101 (MQ=255)
                      tcggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTc            >  2:477297/5‑101 (MQ=255)
                       cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCTTGCGTTCACTTCTCCCGCGACCAGTCGGCCACGTTCCAGTATTCGATGTCCCAGGCGTTCa           >  1:3912497/4‑101 (MQ=255)
                       cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:1880321/4‑101 (MQ=255)
                       cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:2505871/4‑101 (MQ=255)
                       cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  1:3100470/4‑101 (MQ=255)
                       cggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCa           >  2:1061265/4‑101 (MQ=255)
                        ggaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAt          >  2:157641/3‑101 (MQ=255)
                         gaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc         >  1:784488/2‑101 (MQ=255)
                         gaCCGGCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCAtc         >  1:4723792/2‑101 (MQ=255)
                              gCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  2:3904684/1‑101 (MQ=255)
                              gCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  1:3760342/1‑101 (MQ=255)
                              gCCCTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    >  1:4402848/1‑101 (MQ=255)
                              ccccTGCCGGT.CAGACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGAc    <  1:4230807/100‑1 (MQ=255)
                               cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  2:1248577/1‑101 (MQ=255)
                               cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  2:4218159/1‑101 (MQ=255)
                               cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  1:3583558/1‑101 (MQ=255)
                               cccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACg   >  1:2817913/1‑101 (MQ=255)
                                ccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  2:1830114/1‑101 (MQ=255)
                                ccTGCCGGTCC.GACCC.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCACAGGCGTTCATCTCGACGc  >  2:4190492/1‑101 (MQ=255)
                                ccTGCCGGTCC.GA.CCCTACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGc  >  2:1696554/1‑101 (MQ=255)
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CCGCAGCGCCCCCCCCCCCCTGGGGAACCGGCCCTGCCGGT.C.GA.CG.TACGGTCTCTTATCCTGCGATCACTTCGCCCGCGACCAGTCGGCCACGTTCCAGTATTCGCTGTCCCAGGCGTTCATCTCGACGC  >  NC_014034/774600‑774730

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG