Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 78,028 G→A 100% G150E (GGG→GAG)  aroE → shikimate 5‑dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_01403478,0280G→A100.0% 813.7 214G150E (GGG→GAG) aroEshikimate 5‑dehydrogenase
Reads supporting (aligned to +/- strand):  new base (81/133):  ref base (0/0):  total (81/133)

Only 100 of 220 total aligned reads displayed.

CCCGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGGGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGGATG  >  NC_014034/77930‑78125
                                                                                                  |                                                                                                 
cccGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGa                                                                                                 <  2:4469893/101‑1 (MQ=255)
 ccGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGAt                                                                                                <  1:4745336/101‑1 (MQ=255)
 ccGGGCGGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGAt                                                                                                <  2:1523850/101‑1 (MQ=255)
  cGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATc                                                                                               <  1:3077837/101‑1 (MQ=255)
   gggCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATcc                                                                                              >  1:3397471/1‑101 (MQ=255)
     gCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGc                                                                                            <  1:3644355/101‑1 (MQ=255)
      cgggCAGGCCAAGGCGGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCa                                                                                           <  2:1220004/99‑1 (MQ=255)
      cTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCa                                                                                           <  2:180349/101‑1 (MQ=255)
       tGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAt                                                                                          <  2:208936/101‑1 (MQ=255)
         gCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCAttt                                                                                        <  1:2818707/101‑1 (MQ=255)
          cAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTc                                                                                       >  2:869451/1‑101 (MQ=255)
          cAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTc                                                                                       >  2:4685997/1‑101 (MQ=255)
           aGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTcc                                                                                      <  2:4328121/101‑1 (MQ=255)
            ggCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCa                                                                                     <  2:2493735/101‑1 (MQ=255)
            ggCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCa                                                                                     >  1:2775731/1‑101 (MQ=255)
             gCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCaa                                                                                    >  2:1582014/1‑101 (MQ=255)
               cAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAcc                                                                                  <  1:4411372/101‑1 (MQ=255)
               cAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAcc                                                                                  <  1:4500819/101‑1 (MQ=255)
                agggCTGCCCCCGCTGCCGTCAACGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCCTTTCCAAccg                                                                                 <  1:4100805/99‑1 (MQ=255)
                aaGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccg                                                                                 <  1:2844906/101‑1 (MQ=255)
                aaGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccg                                                                                 >  2:3359365/1‑101 (MQ=255)
                aaGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccg                                                                                 <  2:1792059/101‑1 (MQ=255)
                 aGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgc                                                                                >  1:2723350/1‑101 (MQ=255)
                  ggCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgca                                                                               >  1:2485489/1‑101 (MQ=255)
                     tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            <  2:2211059/101‑1 (MQ=255)
                     tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            <  2:3788563/101‑1 (MQ=255)
                     tGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcaccc                                                                            <  2:3736238/101‑1 (MQ=255)
                      ggCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccg                                                                           <  1:924950/101‑1 (MQ=255)
                      ggCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccg                                                                           >  1:3640013/1‑101 (MQ=255)
                        ccccGCCGCCGTCATCGGCGCAGGGGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgca                                                                         >  1:3884125/1‑101 (MQ=255)
                        ccccGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgca                                                                         <  2:3703626/101‑1 (MQ=255)
                        ccccGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccaca                                                                         <  1:3510976/101‑1 (MQ=255)
                         cccGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAAccgcacccgcac                                                                        <  2:4159742/101‑1 (MQ=255)
                          ccgccgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACcgcacccgcacc                                                                       <  1:130677/101‑1 (MQ=255)
                          ccgccgccgTCATCGGCGAAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACcgcacccgcacc                                                                       >  2:4163759/1‑101 (MQ=255)
                           cgccgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCgcccccgcaccc                                                                      >  2:4223841/1‑101 (MQ=255)
                            gccgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGcacccgcacccg                                                                     >  2:1923456/1‑101 (MQ=255)
                             ccgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCgg                                                                    <  1:76744/101‑1 (MQ=255)
                             ccgccgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCgg                                                                    <  1:1438623/101‑1 (MQ=255)
                                 cgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCg                                                                >  2:1057052/1‑101 (MQ=255)
                                 cgTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCg                                                                <  1:1581768/101‑1 (MQ=255)
                                  gTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGa                                                               <  1:129007/101‑1 (MQ=255)
                                  gTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGa                                                               >  1:1818556/1‑101 (MQ=255)
                                  gTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGa                                                               <  1:1943371/101‑1 (MQ=255)
                                    cATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAgg                                                             <  2:4133006/101‑1 (MQ=255)
                                       cGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGc                                                          <  2:597521/101‑1 (MQ=255)
                                       cGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGc                                                          >  1:3336833/1‑101 (MQ=255)
                                        ggCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGGGCt                                                         >  2:2991426/1‑101 (MQ=255)
                                          cgcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCttt                                                       >  2:892112/1‑101 (MQ=255)
                                          cgcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCttt                                                       <  1:3188283/101‑1 (MQ=255)
                                           gcAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTg                                                      <  1:519426/101‑1 (MQ=255)
                                            cAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTgc                                                     >  1:2132193/1‑101 (MQ=255)
                                               gCGGTGCCGCCCGCACCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGcc                                                  <  1:1094149/101‑1 (MQ=255)
                                                cGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCg                                                 <  2:2743444/101‑1 (MQ=255)
                                                cGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCg                                                 >  2:2110591/1‑101 (MQ=255)
                                                  gTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGaa                                               <  2:644852/101‑1 (MQ=255)
                                                  gTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGaa                                               <  1:4321505/101‑1 (MQ=255)
                                                  gTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGaa                                               <  2:2643030/101‑1 (MQ=255)
                                                   tGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAAt                                              <  1:631295/101‑1 (MQ=255)
                                                   tGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAAt                                              <  2:286862/101‑1 (MQ=255)
                                                    gccgccCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAAtt                                             <  2:3639784/101‑1 (MQ=255)
                                                     ccgcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTc                                            <  1:1008202/101‑1 (MQ=255)
                                                       gcccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCgg                                          <  1:913179/101‑1 (MQ=255)
                                                        cccgcGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGc                                         >  1:2389573/1‑101 (MQ=255)
                                                            cgcCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGccccc                                     <  2:292344/101‑1 (MQ=255)
                                                            cgcCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGCGCTTTGCGCCGAATTCGGccccc                                     >  2:483348/1‑101 (MQ=255)
                                                               cATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCGCCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCcgcg                                  <  1:3716612/101‑1 (MQ=255)
                                                                   gtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcgt                              <  1:830399/101‑1 (MQ=255)
                                                                   gtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcgt                              >  1:482504/1‑101 (MQ=255)
                                                                   gtcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcgt                              >  1:4715912/1‑101 (MQ=255)
                                                                   gtcgtcgCCCTGCCCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCCCCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGcgtcgt                              <  1:4391966/101‑1 (MQ=255)
                                                                    tcgtcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTg                             <  2:3034556/101‑1 (MQ=255)
                                                                       tcgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGtt                          <  2:3557182/101‑1 (MQ=255)
                                                                        cgCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTc                         >  2:3832077/1‑101 (MQ=255)
                                                                         gCCCTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCACCCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCa                        <  2:3225128/101‑1 (MQ=255)
                                                                          cccTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAg                       <  1:2704906/101‑1 (MQ=255)
                                                                          cccTGCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGACCCCGCGTCGTGGTTCAg                       <  1:3359567/101‑1 (MQ=255)
                                                                              gCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGAtt                   >  2:3753348/1‑101 (MQ=255)
                                                                              gCTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGAtt                   <  1:4164005/101‑1 (MQ=255)
                                                                               cTCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTg                  >  1:2259155/1‑101 (MQ=255)
                                                                                tCGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTgg                 >  1:2540234/1‑101 (MQ=255)
                                                                                 cGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCGCCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTggg                <  1:154039/101‑1 (MQ=255)
                                                                                 cGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTggg                >  2:3053784/1‑101 (MQ=255)
                                                                                 cGAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTggg                >  2:1972333/1‑101 (MQ=255)
                                                                                  gAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGga               >  2:2041569/1‑101 (MQ=255)
                                                                                  gAGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGga               >  2:3666588/1‑101 (MQ=255)
                                                                                   aGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGgag              <  2:2775566/101‑1 (MQ=255)
                                                                                   aGGCCGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGgag              >  2:435126/1‑101 (MQ=255)
                                                                                       cGGTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAgg          <  1:2990149/101‑1 (MQ=255)
                                                                                        ggTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGt         <  2:4645900/101‑1 (MQ=255)
                                                                                         gTTGCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTc        <  2:3380971/101‑1 (MQ=255)
                                                                                            gCCCGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCggg     <  2:1370429/101‑1 (MQ=255)
                                                                                             cccGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGga    >  2:2506569/1‑101 (MQ=255)
                                                                                             cccGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGga    <  2:2723196/101‑1 (MQ=255)
                                                                                              ccGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgat   >  1:881652/1‑101 (MQ=255)
                                                                                               cGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgatg  <  1:4686253/101‑1 (MQ=255)
                                                                                               cGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgatg  <  2:3081612/101‑1 (MQ=255)
                                                                                               cGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgatg  <  1:4365201/101‑1 (MQ=255)
                                                                                               cGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgatg  >  1:4625794/1‑101 (MQ=255)
                                                                                               cGGAGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGgatg  <  1:1030498/101‑1 (MQ=255)
                                                                                                  |                                                                                                 
CCCGGGCTGGCAGGCCAAGGCTGGCCCCGCCGCCGTCATCGGCGCAGGCGGTGCCGCCCGCGCCATCGTCGTCGCCCTGCTCGAGGCCGGTTGCCCGGGGATCCGCATTTCCAACCGCACCCGCACCCGGGCCGAGGTGCTTTGCGCCGAATTCGGCCCCCGCGTCGTGGTTCAGGATTGGGAGAAGGTCGGGATG  >  NC_014034/77930‑78125

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG