Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	974,361	C→G	100%	intergenic (+241/+18)	RCAP_rcc00884 → / ← RCAP_rcc00885	hypothetical protein/hypothetical protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	974,361	0	C→G	100.0%	186.2	48	intergenic (+241/+18)	RCAP_rcc00884/RCAP_rcc00885	hypothetical protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (33/15):  ref base (0/0):  total (33/15)

TGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974278‑974458                                                                                        |                                                                                                  tGTTTTCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGCGCCCCGGCGGCCGCCGCGGCGGAAGACCCCGCCCGCCACGGCCg                                                                                      >  1:2726051/1‑101 (MQ=2)   ttttCACCTTGCGACAAATATCCCGGGGGTCCGGGGGCGGAGCCCCCGGCGGGGGGGCTCCGCGCCAGCCGAGGCGAAAAAACCCCACCGCCACCGGCggg                                                                                    >  2:4487285/1‑100 (MQ=9)                                    ggggcgacccccccGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGcgcgcg                                                   <  2:3941088/92‑1 (MQ=38)                                      ggcggaGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGAt                                                 <  1:4749927/96‑1 (MQ=255)                                      gagggacccccgGGCGGGTCGCCGCGGCGACAGCCGAGGCGAAATGCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGAt                                                 <  2:2316515/94‑1 (MQ=255)                                       gcggaccccccGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATc                                                <  2:2312010/95‑1 (MQ=255)                                                ggggcggGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAgg                                       <  1:958662/99‑1 (MQ=255)                                                ccGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAgg                                       <  1:1589831/101‑1 (MQ=255)                                                     gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcat                                  >  2:3224233/1‑101 (MQ=255)                                                     gggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGgcat                                  >  2:958466/1‑101 (MQ=255)                                                      ggTCGCCTCGGCGACAGCCGAGGCGAAATACGCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCCGGGTCGAGGGCGCGATCACCCCGAGGGccagc                                 >  2:3711130/1‑99 (MQ=255)                                                      ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 <  2:3387407/101‑1 (MQ=255)                                                      ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 >  2:3574197/1‑101 (MQ=255)                                                      ggTCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGtcatc                                 >  2:826376/1‑101 (MQ=255)                                                        tCGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAg                               >  1:1341062/1‑101 (MQ=255)                                                         cGCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGGTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgc                              >  1:4003625/1‑101 (MQ=255)                                                          gCCTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAgcg                             >  2:3720723/1‑101 (MQ=255)                                                            cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  2:1197536/1‑101 (MQ=255)                                                            cTCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCa                           >  2:156902/1‑101 (MQ=255)                                                             tCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          >  2:3647201/1‑101 (MQ=255)                                                             tCGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          >  2:244856/1‑101 (MQ=255)                                                             ccGGCGACAGCCGAGGCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCAt                          <  2:2146268/100‑1 (MQ=255)                                                              cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         <  1:4487535/101‑1 (MQ=255)                                                              cGGCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATa                         <  2:2187361/101‑1 (MQ=255)                                                                gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCNCGATCACCCAGAGGGTCATCAGCGCATAgg                       >  1:2804744/1‑101 (MQ=255)                                                                gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       <  1:1317319/101‑1 (MQ=255)                                                                gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       >  1:1817695/1‑101 (MQ=255)                                                                gCGACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAgg                       <  1:42874/101‑1 (MQ=255)                                                                  gACAGCCGAGGCGAAATGCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     <  1:4503420/101‑1 (MQ=255)                                                                  gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGCACAGGATCAGGTTTGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  2:4478335/1‑101 (MQ=255)                                                                  gACAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGcc                     >  2:1389093/1‑101 (MQ=255)                                                                   aCAGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCg                    >  2:4144330/1‑101 (MQ=255)                                                                    cAGCCCAGGCGAAATACGCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGAGCAGGTGCGAGTGCGCGATCACCCAGGGGACCATCAGCGCATAGGCCGc                   >  2:4718195/1‑101 (MQ=255)                                                                     aGCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCa                  <  2:2725897/101‑1 (MQ=255)                                                                      gCCGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaa                 <  2:368946/101‑1 (MQ=255)                                                                       ccGAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGAGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCaaa                >  2:202826/1‑101 (MQ=255)                                                                         gAGGCGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAAcc              >  2:4501029/1‑101 (MQ=255)                                                                            gCGAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCg           <  2:1215175/101‑1 (MQ=255)                                                                             cGAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGc          >  2:1739154/1‑101 (MQ=255)                                                                              gAAATCCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         <  2:701704/101‑1 (MQ=255)                                                                              gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  1:4739236/1‑101 (MQ=255)                                                                              gAAATACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGcc         >  2:4003938/1‑101 (MQ=255)                                                                               aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  2:2072633/1‑101 (MQ=255)                                                                               aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  2:1435326/1‑101 (MQ=255)                                                                               aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  1:4452758/1‑101 (MQ=255)                                                                               aaaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCa        >  1:4684589/1‑101 (MQ=255)                                                                                aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       >  2:2826269/1‑101 (MQ=255)                                                                                aaTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAg       >  2:650850/1‑101 (MQ=255)                                                                                 aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc      >  1:4225744/1‑101 (MQ=255)                                                                                 aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgc      >  1:1376314/1‑101 (MQ=255)                                                                                 aTACCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGCCATCAGCGCATAGGCCCCAAACCCCGCCAgc      >  2:4310627/1‑101 (MQ=255)                                                                                   aCCCCGGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAgcgc    >  1:2038778/1‑101 (MQ=255)                                                                                     cccgGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGccc  >  1:3461710/1‑101 (MQ=255)                                                                                        |                                                                                                  TGTTTTCACCTTGCGACAAATATCCC.GGGGT..GTGCG.GAAGCCCCCGGCGGGTCGCCTCGGCGACAGCCGAGGCGAAATACCCCCGCCGCAACGGCCGGTTCTCAGCCGAACAGGATCAGGTTCGAGCGCGCGATCACCCAGAGGGTCATCAGCGCATAGGCCGCAAACCCCGCCAGCGCCC  >  NC_014034/974278‑974458 Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG