Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,794,301 2 bp→GC 100% coding (1193‑1194/1770 nt) exaA2 → quinoprotein ethanol dehydrogenase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,794,3010C→G100.0% 53.5 205A398G (GCG→GGG) exaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (83/100):  ref base (3/19):  total (86/119)
Fisher's exact test strand distribution p-value = 5.13e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00
*NC_0140341,794,3020G→C100.0% 53.1 205A398A (GCG→GCCexaA2quinoprotein ethanol dehydrogenase
Reads supporting (aligned to +/- strand):  new base (83/100):  ref base (3/19):  total (86/119)
Fisher's exact test strand distribution p-value = 5.13e-03
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 209 total aligned reads displayed.

GAAGAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794206‑1794395
                                                                                                ||                                                                                             
    aaCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAggg                                                                                       >  1:2728066/1‑101 (MQ=255)
     aCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGc                                                                                      >  1:2560309/1‑101 (MQ=255)
     aCATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGc                                                                                      >  2:669500/1‑101 (MQ=255)
      cATCGCCTGGGCCAGGGGCGTCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGcc                                                                                     <  2:4011261/101‑1 (MQ=255)
      cATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGcc                                                                                     >  2:1860683/1‑101 (MQ=255)
      cATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGcc                                                                                     <  2:4090500/101‑1 (MQ=255)
       agcACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCca                                                                                    <  2:2877381/99‑1 (MQ=255)
       aTCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCAGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCca                                                                                    >  1:1045907/1‑101 (MQ=39)
        tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCTCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   <  1:4204906/101‑1 (MQ=39)
        tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   <  2:3186528/101‑1 (MQ=255)
        tCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCcag                                                                                   >  2:4310843/1‑101 (MQ=255)
             tGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGAt                                                                              >  2:3076379/1‑101 (MQ=255)
              gggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATc                                                                             <  1:3102150/101‑1 (MQ=255)
              gggCCAAGGGCATCGATAAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATc                                                                             >  2:4204039/1‑101 (MQ=255)
               ggCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGCTCt                                                                            >  1:2133050/1‑101 (MQ=255)
                gCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCtt                                                                           <  1:3144181/101‑1 (MQ=255)
                 ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCttt                                                                          <  1:4359394/101‑1 (MQ=255)
                 ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCttt                                                                          <  2:734663/101‑1 (MQ=255)
                 ccAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCttt                                                                          <  1:2485184/101‑1 (MQ=255)
                  cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  1:1237098/101‑1 (MQ=255)
                  cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:2099995/101‑1 (MQ=255)
                  cAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCAGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTg                                                                         <  2:1421861/101‑1 (MQ=255)
                   aaGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGc                                                                        >  1:1371583/1‑101 (MQ=255)
                   aaGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGc                                                                        >  2:4457639/1‑101 (MQ=255)
                     gggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCg                                                                      >  1:2539646/1‑101 (MQ=255)
                      ggCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGt                                                                     >  2:3623827/1‑101 (MQ=255)
                       gcctCGATGAAACCGGCCGTCCGATCTAAAACGAGGAGAACCGCCCCGGTGCCCCCGATCCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTg                                                                    <  2:4250695/98‑1 (MQ=255)
                       gCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTg                                                                    >  1:2966259/1‑101 (MQ=255)
                       gCATCGATGAAACCGCCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTg                                                                    <  1:51767/101‑1 (MQ=255)
                          tCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGccc                                                                 >  1:1497842/1‑101 (MQ=255)
                          tCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGccc                                                                 >  2:3089758/1‑101 (MQ=255)
                           cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                <  2:2967581/101‑1 (MQ=255)
                           cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                <  2:3055347/101‑1 (MQ=255)
                           cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                >  1:1941550/1‑101 (MQ=255)
                           cGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCt                                                                >  2:3250626/1‑101 (MQ=255)
                             aTGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCg                                                              <  1:3295728/101‑1 (MQ=255)
                               gAAACCGGCCGTCTGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGtt                                                            <  1:4083905/101‑1 (MQ=255)
                               gAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGtt                                                            >  2:3701751/1‑101 (MQ=255)
                                  aCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCt                                                         <  2:3940506/101‑1 (MQ=255)
                                  aCCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCt                                                         >  1:2217016/1‑101 (MQ=255)
                                   ccGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTg                                                        <  2:194300/101‑1 (MQ=255)
                                   ccGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTg                                                        <  2:3483321/101‑1 (MQ=255)
                                   ccGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTg                                                        >  2:3390735/1‑101 (MQ=255)
                                         gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  2:2768055/1‑101 (MQ=255)
                                         gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  2:1333756/1‑101 (MQ=255)
                                         gTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGggcggc                                                  >  1:4111261/1‑101 (MQ=255)
                                          tCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCa                                                 <  2:272531/101‑1 (MQ=255)
                                           ccGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCaa                                                >  1:413739/1‑101 (MQ=255)
                                            cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  2:4074555/101‑1 (MQ=255)
                                            cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               >  1:1134688/1‑101 (MQ=255)
                                            cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  1:4268972/101‑1 (MQ=255)
                                            cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  1:3573982/101‑1 (MQ=255)
                                            cGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAg                                               <  1:4164800/101‑1 (MQ=255)
                                              aTCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGaa                                             >  1:37690/1‑101 (MQ=255)
                                               tCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAAGGCGGCC.AGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAAc                                            <  2:4110201/101‑1 (MQ=255)
                                               tCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAAc                                            <  2:2403736/101‑1 (MQ=255)
                                               tCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAAc                                            >  2:4672605/1‑101 (MQ=255)
                                                cTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACt                                           <  1:2219266/101‑1 (MQ=255)
                                                 tACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGCACTg                                          >  2:1773840/1‑101 (MQ=255)
                                                 tACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTCTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTg                                          >  1:4094395/1‑101 (MQ=255)
                                                  aCAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTgg                                         >  1:1206871/1‑101 (MQ=255)
                                                  aCAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGACGTGCCCTCGTTCCTGGGCGGCAAGAACTgg                                         >  1:4534304/1‑101 (MQ=255)
                                                   cAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGa                                        >  1:3051549/1‑101 (MQ=255)
                                                   aaacgggggaaaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGa                                        <  2:3219385/91‑1 (MQ=255)
                                                    aaCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGAt                                       >  2:3681627/1‑101 (MQ=255)
                                                    aaCGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGAt                                       <  1:1073564/101‑1 (MQ=255)
                                                      cGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGc                                     <  1:81427/101‑1 (MQ=255)
                                                       gaggagAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGcc                                    <  1:1575687/101‑1 (MQ=255)
                                                           agaACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATg                                >  1:2362392/1‑101 (MQ=255)
                                                             aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.TGCGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              <  1:4063712/101‑1 (MQ=255)
                                                             aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              <  1:778060/101‑1 (MQ=255)
                                                             aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              <  1:687466/101‑1 (MQ=255)
                                                             aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              <  2:3063377/101‑1 (MQ=255)
                                                             aaCCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGc                              >  2:1521227/1‑101 (MQ=255)
                                                               ccGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCt                            >  1:2584245/1‑101 (MQ=255)
                                                                cGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           <  1:4660798/101‑1 (MQ=255)
                                                                cGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTa                           <  2:2606214/101‑1 (MQ=255)
                                                                 gCCCCGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTAc                          <  2:1007747/101‑1 (MQ=255)
                                                                  ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  2:3352261/101‑1 (MQ=255)
                                                                  ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  1:1288651/101‑1 (MQ=255)
                                                                  ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  2:2539501/101‑1 (MQ=255)
                                                                  ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         <  2:4538469/101‑1 (MQ=255)
                                                                  ccccGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACa                         >  1:3334234/1‑101 (MQ=255)
                                                                     cGGTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGcc                      <  2:811684/101‑1 (MQ=255)
                                                                       gTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  2:3874259/101‑1 (MQ=255)
                                                                       gTGCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAg                    <  2:2661021/101‑1 (MQ=255)
                                                                         gCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAc                  <  1:3899471/101‑1 (MQ=255)
                                                                         gCCCCCGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAc                  <  1:34360/101‑1 (MQ=255)
                                                                          cccccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGAcc                 <  1:1649573/101‑1 (MQ=255)
                                                                            cccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCAc               <  1:4311090/101‑1 (MQ=255)
                                                                            cccGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCAc               <  2:2678911/101‑1 (MQ=255)
                                                                              cGATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACgg             <  2:4626968/101‑1 (MQ=255)
                                                                               gATGCCGCGGCCGAAGGCGG.CAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACggg            >  2:3902362/1‑101 (MQ=255)
                                                                               gATGCCGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACggg            <  2:463717/101‑1 (MQ=255)
                                                                                   ccGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCctc        <  1:2833662/101‑1 (MQ=38)
                                                                                   ccGCGGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCctc        <  2:3716985/101‑1 (MQ=38)
                                                                                    cgcgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCctct       >  1:1434680/1‑101 (MQ=255)
                                                                                     gcgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtctt      <  1:3681856/101‑1 (MQ=255)
                                                                                     gcgGCCGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCtctt      >  1:1834310/1‑101 (MQ=38)
                                                                                         ccGAA.GGCGGCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGCCTCTTCTAt  >  2:1580162/1‑101 (MQ=37)
                                                                                                ||                                                                                             
GAAGAACATCACCTGGGCCAAGGGCATCGATGAAACCGGCCGTCCGATCTACAACGAGGAGAACCGCCCCGGTGCCCCCGATGCCGCGGCCGAA.GCGGCCAAGGGCCAGCAGATCTTTGCCGTGCCCTCGTTCCTGGGCGGCAAGAACTGGATGCCGATGGCCTACAGCCAGACCACGGGGCTCTTCTAT  >  NC_014034/1794206‑1794395

Base quality scores:  ATCG  < 3 ≤  ATCG  < 23 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG