Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,802,243 2 bp→GC 100% coding (626‑627/2124 nt) ligA → DNA ligase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,802,2430C→G100.0% 716.7 197T209R (ACG→AGG) ligADNA ligase
Reads supporting (aligned to +/- strand):  new base (99/98):  ref base (0/0):  total (99/98)
*NC_0140341,802,2440G→C100.0% 737.9 197T209T (ACG→ACCligADNA ligase
Reads supporting (aligned to +/- strand):  new base (99/98):  ref base (0/0):  total (99/98)

Only 100 of 209 total aligned reads displayed.

GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338
                                                                                               ||                                                                                              
ggCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCg                                                                                            <  2:1970131/101‑1 (MQ=255)
  cgcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGcc                                                                                          <  2:2597287/101‑1 (MQ=255)
   gcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCa                                                                                         >  2:1446943/1‑101 (MQ=255)
   gcCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCa                                                                                         <  1:3110773/101‑1 (MQ=255)
    ccTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCaa                                                                                        >  2:3790719/1‑101 (MQ=255)
     cTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAAt                                                                                       >  1:3588851/1‑101 (MQ=255)
      tGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATc                                                                                      <  1:2358258/101‑1 (MQ=255)
        ccGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcg                                                                                    <  2:2365959/101‑1 (MQ=255)
        ccGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcg                                                                                    <  2:2845690/101‑1 (MQ=255)
         cGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcgg                                                                                   >  2:1200602/1‑100 (MQ=255)
         cGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATCCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcgc                                                                                   <  1:2103564/101‑1 (MQ=255)
          gTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCcgcg                                                                                  <  2:4501582/101‑1 (MQ=255)
           tGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGCCGGAGCGGGGCGACAAGAGCTTCGCCAATCCgcgc                                                                                 <  2:3181897/101‑1 (MQ=255)
            gCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCa                                                                                <  1:2681045/101‑1 (MQ=255)
             cTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCCGGGCGACAAGAGCTTCGCCAATCCGCGCaa                                                                               >  2:1872837/1‑101 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAAt                                                                              >  1:1885707/1‑101 (MQ=255)
              tGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGAAGGCGGAGCGGGGCGACAAGAGCTTCGACAATCCGCGCAAt                                                                              >  1:3152451/1‑101 (MQ=255)
                gAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgc                                                                            <  1:2069085/101‑1 (MQ=255)
                 aaGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATgcc                                                                           <  1:3197002/101‑1 (MQ=255)
                     ccGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGccgccg                                                                       >  1:4480385/1‑101 (MQ=255)
                      cgcgGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCcgccgc                                                                      >  1:3894970/1‑101 (MQ=255)
                      cgcgGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCcgccgc                                                                      <  1:178854/101‑1 (MQ=255)
                       ncggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGTAATgctncnccn                                                                     >  1:1009164/2‑94 (MQ=255)
                         ggcgAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCggg                                                                   >  1:2007171/1‑101 (MQ=255)
                           ggAGGTCTACATGAG.CATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTc                                                                <  2:277240/100‑1 (MQ=255)
                            gAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTc                                                                <  2:4738336/101‑1 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               <  2:2671668/101‑2 (MQ=255)
                             aGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcg                                                               <  1:1446348/101‑2 (MQ=255)
                              ggTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCttgc                                                              >  2:4001277/1‑98 (MQ=255)
                              ggTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgc                                                              >  2:1220602/1‑99 (MQ=255)
                              ggTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgc                                                              >  1:1568796/1‑99 (MQ=255)
                              ggTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgc                                                              >  2:4056420/1‑99 (MQ=255)
                                tCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTcgctg                                                            <  1:3558861/101‑5 (MQ=255)
                                  tACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcg                                                          <  1:866916/101‑1 (MQ=255)
                                   aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                         >  1:2044803/1‑101 (MQ=255)
                                   aCATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTgcgc                                                         >  1:3762135/1‑101 (MQ=255)
                                    cATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGcc                                                        >  2:426745/1‑101 (MQ=255)
                                      tGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAg                                                      <  2:4429091/101‑1 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGTCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                    <  2:2204460/101‑1 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                    >  2:157369/1‑101 (MQ=255)
                                        aGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCt                                                    >  2:4686618/1‑101 (MQ=255)
                                         gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                   <  2:2537672/101‑1 (MQ=255)
                                         gCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTc                                                   >  1:331709/1‑101 (MQ=255)
                                          ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                  <  1:2452027/101‑1 (MQ=255)
                                          ccATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCg                                                  >  1:756722/1‑101 (MQ=255)
                                            aTGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGAc                                                <  2:3288552/101‑1 (MQ=255)
                                               ccGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCCGGCTCGCTGCGCCAGCTCGACGcc                                             >  2:2146387/1‑101 (MQ=255)
                                                 gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGTGCAATGCCGCCGCGGGCTCGCTGCGGCAGCTCGACGCCCg                                           >  1:4715153/1‑101 (MQ=255)
                                                 gATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCg                                           >  1:1466116/1‑101 (MQ=255)
                                                  aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                          >  2:3767382/1‑101 (MQ=255)
                                                  aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                          >  1:1064441/1‑101 (MQ=255)
                                                  aTTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCgg                                          >  2:2111372/1‑101 (MQ=255)
                                                   ttttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGa                                         <  1:967255/101‑1 (MQ=255)
                                                    tttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGAt                                        >  1:529884/1‑101 (MQ=255)
                                                     ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                       >  1:1975721/1‑101 (MQ=255)
                                                     ttGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATc                                       <  2:4076738/101‑1 (MQ=255)
                                                      tGCGGCGCTCAATGGGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCGAATCCGCGCAGTGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCAGATCa                                      <  2:3152251/101‑1 (MQ=255)
                                                         ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCg                                   >  1:1731350/1‑101 (MQ=255)
                                                         ggcgCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCAACg                                   >  1:4243897/1‑101 (MQ=255)
                                                           cgcTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGTCGCCCGGATCACCGcc                                 >  2:2557465/1‑101 (MQ=255)
                                                            ggtGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCt                                <  2:861879/99‑1 (MQ=255)
                                                              tGAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTcc                              >  2:479755/1‑101 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             >  2:3323752/1‑101 (MQ=255)
                                                               gAATGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCa                             <  1:4070929/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            >  2:3750293/1‑101 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:459119/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:2836433/101‑1 (MQ=255)
                                                                aaTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAg                            <  1:1750875/101‑1 (MQ=255)
                                                                 aTGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGa                           <  1:2755935/101‑1 (MQ=255)
                                                                  tGCGCGGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGAc                          <  1:3705265/101‑1 (MQ=255)
                                                                     gcgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCt                       <  2:1526394/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  2:3894728/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  1:1346573/101‑1 (MQ=255)
                                                                      cgGCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCtt                      <  2:1844303/101‑1 (MQ=255)
                                                                       ggCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTg                     <  1:1220692/101‑1 (MQ=255)
                                                                        gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                    <  1:3222123/101‑1 (MQ=255)
                                                                        gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                    <  1:3947140/101‑1 (MQ=255)
                                                                        gCAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGa                    <  1:4686874/101‑1 (MQ=255)
                                                                         cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                   >  1:845918/1‑101 (MQ=255)
                                                                         cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                   <  2:4625934/101‑1 (MQ=255)
                                                                         cAGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaa                   >  2:2011438/1‑101 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  >  2:3568984/1‑101 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  <  1:1657118/101‑1 (MQ=255)
                                                                          aGGCGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGaaa                  <  1:2146511/101‑1 (MQ=255)
                                                                             cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc               >  2:7882/1‑101 (MQ=255)
                                                                             cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc               >  1:1199226/1‑101 (MQ=255)
                                                                             cGGAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTc               >  2:1191490/1‑101 (MQ=255)
                                                                              ggAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCt              <  1:1817703/101‑1 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             <  2:4549809/101‑1 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             >  2:1485709/1‑101 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             <  2:1752358/101‑1 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCtt             >  2:715197/1‑101 (MQ=255)
                                                                               gAGCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTAtt             <  1:3790954/101‑1 (MQ=255)
                                                                                 gCGGGGCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTg           <  1:4349871/101‑1 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        >  2:248367/1‑101 (MQ=255)
                                                                                    gggCGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCt        <  2:3124278/101‑1 (MQ=255)
                                                                                       ccaCAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     <  2:589713/99‑1 (MQ=255)
                                                                                       cGACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTAtt     >  1:3857741/1‑101 (MQ=255)
                                                                                        gACAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTc    <  1:4418277/101‑1 (MQ=255)
                                                                                          cAAGAGCTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCGCTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCt  >  1:2993862/1‑101 (MQ=255)
                                                                                               ||                                                                                              
GGCGCCTGCCGTGCTGGAAGTCCGCGGCGAGGTCTACATGAGCCATGCCGATTTTGCGGCGCTGAATGCGCGGCAGGCGGAGCGGGGCGACAAGACGTTCGCCAATCCGCGCAATGCCGCCGCGGGCTCCGTGCGCCAGCTCGACGCCCGGATCACCGCCTCCAGACCCTTGAAATTCTTTGCCTATTCCT  >  NC_014034/1802148‑1802338

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG