Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,053,078 2 bp→GC 100% coding (4156‑4157/6198 nt) RCAP_rcc01900 ← hemolysin‑type calcium‑binding repeat family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,053,0780C→G100.0% 643.7 175R1386P (CGG→CCG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (89/85):  ref base (0/1):  total (89/86)
*NC_0140342,053,0790G→C100.0% 649.0 175R1386G (CGG→GGG) RCAP_rcc01900hemolysin‑type calcium‑binding repeat family protein
Reads supporting (aligned to +/- strand):  new base (89/86):  ref base (0/0):  total (89/86)

Only 100 of 188 total aligned reads displayed.

GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACGGT  >  NC_014034/2052983‑2053175
                                                                                               ||                                                                                                
ggATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCg                                                                                              <  2:1204965/101‑1 (MQ=255)
   tGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCg                                                                                           <  1:4523818/101‑1 (MQ=255)
      cGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAgg                                                                                        >  2:4217132/1‑101 (MQ=255)
       gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGTGGa                                                                                       >  2:1517069/1‑101 (MQ=255)
       gTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCCTTCGCCGAGGa                                                                                       <  1:2572477/101‑1 (MQ=255)
        ttGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAt                                                                                      <  2:4366193/101‑1 (MQ=255)
        tggCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGAt                                                                                      <  1:3424357/99‑1 (MQ=255)
          gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                    >  1:2882924/1‑101 (MQ=255)
          gCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATgg                                                                                    <  1:1944316/101‑1 (MQ=255)
           cccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgt                                                                                   <  1:474264/101‑1 (MQ=255)
            ccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtg                                                                                  <  1:3063445/101‑1 (MQ=255)
            ccGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGCCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGgtg                                                                                  >  1:3658735/1‑101 (MQ=255)
                gccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGt                                                                              >  1:3883004/1‑101 (MQ=255)
                gccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGt                                                                              >  2:1594860/1‑101 (MQ=255)
                cccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGt                                                                              <  1:1813855/100‑1 (MQ=255)
                 ccgccAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                             <  1:3132498/101‑1 (MQ=255)
                 ccgcaAAGGATGCTGTCTTCATCCGGCCCGCCGGTGATGCTGTCATTGCCCGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGtt                                                                             <  2:1974676/101‑1 (MQ=37)
                     cAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGccc                                                                         >  1:2377411/1‑101 (MQ=255)
                      aaGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCt                                                                        >  1:851102/1‑101 (MQ=255)
                        ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                      >  2:620357/1‑101 (MQ=255)
                        ggATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTcc                                                                      >  1:3603161/1‑101 (MQ=255)
                         gATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTccc                                                                     >  1:2179565/1‑101 (MQ=255)
                           tccTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCg                                                                   <  2:2550417/99‑1 (MQ=255)
                            gCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGc                                                                  >  2:1031280/1‑101 (MQ=255)
                            gCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGc                                                                  >  2:451316/1‑101 (MQ=255)
                             cTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGcc                                                                 >  1:1098985/1‑101 (MQ=255)
                             cTGTCGTCACCCGGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGcc                                                                 <  2:475611/101‑1 (MQ=38)
                              tGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGccc                                                                >  2:4548353/1‑101 (MQ=255)
                               ggcttcATCCCGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCa                                                               <  2:1550736/97‑1 (MQ=37)
                                tcgtcACCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGGCGAGGATGGTGTCGTTGCCCTCCCCGCCCAg                                                              <  1:4217379/101‑1 (MQ=38)
                                 cgtcccccggCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGc                                                             <  1:3264842/92‑1 (MQ=37)
                                  gtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCCTCGCCCGCTTCGCCGAGGATGGTTTCGTTGCCCTCCCCGCCCCGCa                                                            >  2:3705746/1‑101 (MQ=37)
                                  gtcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCa                                                            >  2:4580119/1‑101 (MQ=255)
                                  ctcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCa                                                            <  1:3229085/100‑1 (MQ=255)
                                   tcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAc                                                           >  2:51887/1‑101 (MQ=255)
                                   tcATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAc                                                           >  2:1332041/1‑101 (MQ=255)
                                    cATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                          >  2:403420/1‑101 (MQ=255)
                                    cATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCAcc                                                          >  2:912063/1‑101 (MQ=255)
                                     aTCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCg                                                         <  1:2217942/101‑1 (MQ=255)
                                       ccAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGTCCTCCCCGCCCAGCACCGAg                                                       <  2:3492428/101‑1 (MQ=255)
                                         cggccgccgGGGATTCTGTCTTTGGCCGGGGCACCGCCCCGGGTTTTATTGGCCTCTTCGCCGCGGAAGGGGTCGTTTCCCTCCCCGCCCAGCACCGAGTc                                                     <  2:1440423/98‑1 (MQ=255)
                                         aGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTc                                                     >  1:1059757/1‑101 (MQ=255)
                                          gCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGGGTCATCGCCGCCTTCGCCGTGGTTGGGTTCGTTCCCCCCCCCGCCCAGCACCGAGCCa                                                    >  2:793260/1‑101 (MQ=25)
                                           cccGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCAt                                                   >  2:4117228/1‑101 (MQ=255)
                                             cgccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCg                                                 >  1:3724334/1‑101 (MQ=255)
                                               ccgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGcc                                               >  2:1693053/1‑101 (MQ=255)
                                                cgGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGccc                                              >  2:4662087/1‑101 (MQ=255)
                                                cgGTGACGCCGTCATGGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCGTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCCAGTCATCGccc                                              <  2:3564231/101‑1 (MQ=25)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                             >  2:629260/1‑101 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                             <  2:1059678/101‑1 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                             >  1:2685998/1‑101 (MQ=255)
                                                 ggTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCg                                             <  1:2787559/101‑1 (MQ=255)
                                                  gTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGa                                            <  2:3632660/101‑1 (MQ=255)
                                                   tGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAg                                           >  2:2539298/1‑101 (MQ=255)
                                                    gATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGc                                          >  1:4538534/1‑101 (MQ=255)
                                                     aTGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGcc                                         <  2:2647537/101‑1 (MQ=255)
                                                     aTGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGcc                                         >  1:2563602/1‑101 (MQ=255)
                                                     aTGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCACCACCGAGTCATCGCCCGAGcc                                         >  1:1563113/1‑101 (MQ=255)
                                                      tGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGccc                                        <  1:794444/101‑1 (MQ=255)
                                                       gCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCg                                       >  2:4158850/1‑101 (MQ=255)
                                                        cTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGc                                      <  2:3882762/101‑1 (MQ=255)
                                                        cTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGc                                      >  2:4740196/1‑101 (MQ=255)
                                                        cTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGc                                      <  2:3071574/101‑1 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCt                                    <  1:1597869/101‑1 (MQ=255)
                                                          gTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCt                                    <  1:4449265/101‑1 (MQ=255)
                                                           tCATTGCCCGCCCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtc                                   <  1:3176957/101‑1 (MQ=255)
                                                            cATCGCCCGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcg                                  <  1:1517170/101‑1 (MQ=37)
                                                             aTTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGACCTCCCCGCCCAGCACCGAGTCCTCGCCCGAGCCCGCCtcga                                 >  2:563013/1‑101 (MQ=38)
                                                               tGCCCGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatc                               <  1:1764918/101‑1 (MQ=255)
                                                               tGCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatc                               <  1:499227/101‑1 (MQ=255)
                                                                gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                              <  1:4740452/101‑1 (MQ=255)
                                                                gCCCGCGCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcg                              >  1:1863448/1‑101 (MQ=255)
                                                                 cccGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCtcgatcga                             <  2:2735436/101‑1 (MQ=255)
                                                                  ccGCGCCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGaa                            <  1:1262696/101‑1 (MQ=255)
                                                                   cgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                           >  1:4738217/1‑101 (MQ=255)
                                                                   cgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                           >  2:2189649/1‑101 (MQ=255)
                                                                   cgcgCCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAt                           <  2:2094198/101‑1 (MQ=255)
                                                                    gcgcCCCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                          <  2:2836322/101‑1 (MQ=255)
                                                                    gcgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                          >  1:970226/1‑101 (MQ=255)
                                                                    gcgcCACCGTCCAGCGTGTCACCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtc                          <  2:3253482/101‑1 (MQ=255)
                                                                     ttccgatctCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcg                         <  2:1160660/93‑1 (MQ=255)
                                                                     cgcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcg                         <  1:4652400/101‑1 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                        <  2:1743421/101‑1 (MQ=255)
                                                                      gcCACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgt                        >  2:854587/1‑101 (MQ=255)
                                                                       ccACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtc                       >  2:1404690/1‑101 (MQ=255)
                                                                       ccACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtc                       <  1:4174427/101‑1 (MQ=255)
                                                                       ccACCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgtc                       >  2:1988459/1‑101 (MQ=255)
                                                                         aCCGTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGc                     >  1:2667356/1‑101 (MQ=255)
                                                                            gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg                  >  1:4605671/1‑101 (MQ=255)
                                                                            gTCCAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCgg                  <  1:403464/101‑1 (MQ=255)
                                                                              ccAGCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAAtcgagatcggaa                >  1:1160746/1‑92 (MQ=255)
                                                                                 gCGTGTCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGccc             <  2:2242914/101‑1 (MQ=255)
                                                                                   gtgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc           >  2:4429397/1‑101 (MQ=255)
                                                                                   gtgtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCcgc           <  1:629324/101‑1 (MQ=255)
                                                                                     gtCATCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGt         >  2:1796012/1‑101 (MQ=255)
                                                                                     gtCATCGCCCGCTTCGCCGAGGATGGGGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGt         <  1:1403780/101‑1 (MQ=255)
                                                                                        aTCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCa      >  2:3819307/1‑101 (MQ=255)
                                                                                         tCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCAc     <  1:1902707/101‑1 (MQ=255)
                                                                                         tCGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCAc     <  1:936096/101‑1 (MQ=255)
                                                                                          cGCCCGCTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACg    >  1:73794/1‑101 (MQ=255)
                                                                                               ||                                                                                                
GGATGTCGTTGCCCGCGCCGCCAAGGATGCTGTCGTCATCCAGCCCGCCGGTGATGCTGTCATTGCCCGCGCCACCGTCCAGCGTGTCATCGCCCCGTTCGCCGAGGATGGTGTCGTTGCCCTCCCCGCCCAGCACCGAGTCATCGCCCGAGCCCGCCTCGATCGAATCGTCGCCGGTGCCCGCGTTCACGGT  >  NC_014034/2052983‑2053175

Base quality scores:  ATCG  < 3 ≤  ATCG  < 17 ≤  ATCG  < 25 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG