Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,177,289 2 bp→AT 100% coding (87‑88/990 nt) rbsB ← ribose ABC transporter periplasmic ribose‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,177,2890T→A100.0% 733.5 189T30S (ACA→TCA) rbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (114/75):  ref base (0/0):  total (114/75)
*NC_0140342,177,2900A→T100.0% 708.3 187C29* (TGT→TGArbsBribose ABC transporter periplasmic ribose‑binding protein
Reads supporting (aligned to +/- strand):  new base (112/75):  ref base (0/0):  total (112/75)

Only 100 of 193 total aligned reads displayed.

CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCAT  >  NC_014034/2177202‑2177376
                                                                                              ||                                                                                           
cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAt.cagacag                                                                                     >  1:866589/1‑101 (MQ=255)
cATAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAt.cagacag                                                                                     >  2:2660976/1‑101 (MQ=255)
  tAGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGc                                                                                   >  2:1128810/1‑101 (MQ=255)
   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGcc                                                                                  >  1:3509801/1‑101 (MQ=255)
   aGGTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGcc                                                                                  >  1:531557/1‑101 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGccc                                                                                 <  1:3148854/101‑1 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGccc                                                                                 >  1:10590/1‑101 (MQ=255)
    ggTCGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGccc                                                                                 <  2:3464801/101‑1 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCgg                                                                              >  2:2697323/1‑101 (MQ=255)
       cGAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCgg                                                                              >  2:1896236/1‑101 (MQ=255)
        gAGAGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGATG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGt                                                                             <  1:2753810/101‑1 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                            >  1:712995/4‑101 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                            <  2:2067030/98‑1 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                            <  2:3010575/98‑1 (MQ=255)
         agagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGtt                                                                            >  1:117917/4‑101 (MQ=255)
          gagCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGttt                                                                           <  1:1726189/99‑1 (MQ=255)
           agCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTc                                                                          <  2:2388613/100‑1 (MQ=255)
            gccccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                         <  1:3960182/98‑1 (MQ=255)
            gCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCg                                                                         <  2:398862/101‑1 (MQ=255)
             cACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCgg                                                                        >  1:3329157/1‑101 (MQ=255)
              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                                                       >  1:2966930/1‑101 (MQ=255)
              aCCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGc                                                                       >  1:2238419/1‑101 (MQ=255)
               ccAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGcc                                                                      <  2:1553524/101‑1 (MQ=255)
                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                   <  2:733753/101‑1 (MQ=255)
                 aGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.a                                                                   <  2:2622262/101‑1 (MQ=255)
                   ccccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aag                                                                 >  1:1298642/1‑99 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                               <  1:45216/101‑5 (MQ=255)
                     ccATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagcc                                                               >  2:2176465/1‑97 (MQ=255)
                      cATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCG.aagccg                                                              >  1:2316510/1‑96 (MQ=255)
                        tctcGGTCGCCTTTGCCGAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAgccgcc                                                             <  2:856256/101‑1 (MQ=255)
                          tcGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGcccc                                                           >  2:1297280/1‑101 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                         >  2:129348/1‑101 (MQ=255)
                            ggTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCgg                                                         <  2:2748854/101‑1 (MQ=255)
                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                       <  2:3916553/101‑1 (MQ=255)
                              tCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGcc                                                       >  2:4208834/1‑101 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                      <  1:2194594/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                      <  2:1629364/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                      >  2:2480396/1‑101 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                      <  2:4380719/101‑1 (MQ=255)
                               cGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCa                                                      <  1:90141/101‑1 (MQ=255)
                                gCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAg                                                     <  2:10585/101‑1 (MQ=255)
                                   tttGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCCgccc                                                  >  1:3016848/1‑99 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGAGG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCTCgg                                                >  1:286264/1‑101 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                >  1:2729928/1‑101 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                >  1:476132/1‑101 (MQ=255)
                                     tGCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCgg                                                <  2:2453426/101‑1 (MQ=255)
                                      gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCCGCGCGGa                                               >  2:1821383/1‑101 (MQ=255)
                                      gCCGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGa                                               >  2:3702380/1‑101 (MQ=255)
                                        cGAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaa                                             <  1:604013/101‑1 (MQ=255)
                                         gAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                            <  1:4026424/101‑1 (MQ=255)
                                         gAAGCCCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                            <  1:93628/101‑1 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGaaaa                                            >  2:2384018/1‑101 (MQ=255)
                                         gAAGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCCCCCCGGCCAGCGCGCaaaa                                            >  2:475258/1‑101 (MQ=255)
                                          aaGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAAc                                           >  1:3798736/1‑101 (MQ=255)
                                           aGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                          <  1:4655124/101‑1 (MQ=255)
                                           aGCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACg                                          >  1:2520704/1‑101 (MQ=255)
                                            gCACCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGc                                         <  1:797856/101‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGCT.CAGACAGGCGCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAGAACGccg                                       >  2:1143561/1‑100 (MQ=255)
                                              aCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                       <  2:2131878/101‑1 (MQ=255)
                                              aCCTTCCTTCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCa                                       >  1:1961567/1‑101 (MQ=255)
                                               ccttccttCATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.g                                     >  1:463341/1‑101 (MQ=255)
                                                cttccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gg                                    >  2:4527371/1‑101 (MQ=255)
                                                  tccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                  <  2:646470/101‑3 (MQ=255)
                                                  tccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTT.GGGGATCACACAGTCCCCGGTTTCGGCCGAAGCGGCCCCGGCCAGCGCGGAAAACGCCA.gggc                                  >  1:3901170/1‑99 (MQ=255)
                                                   ccttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggca                                 >  1:2111337/1‑98 (MQ=255)
                                                    cttcATCTTGACGAAAAA..GTGGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCAGCCCCGGCCCGCGCGGAAAACGCCA.gggcag                                <  1:2697477/101‑5 (MQ=255)
                                                    cttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcag                                >  1:3055048/1‑97 (MQ=255)
                                                     ttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                               >  1:224779/1‑96 (MQ=255)
                                                     ttcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCA.gggcaga                               <  1:2914966/101‑6 (MQ=255)
                                                      tcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCagag                               >  1:2454500/1‑101 (MQ=255)
                                                      tcATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCACGGCCAGCGCGGAAAACGCCAGGGCagag                               <  1:2366662/101‑1 (MQ=255)
                                                       cATCTTGAGG.AAAAAGG.GGTTGGTGTCGGTCTTGGCGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                              >  2:127815/1‑101 (MQ=255)
                                                       cATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                              >  1:1018811/1‑101 (MQ=255)
                                                       cATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                              >  1:1656199/1‑101 (MQ=255)
                                                       cATCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAgg                              >  1:1639311/1‑101 (MQ=255)
                                                        aTCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                            >  2:604852/1‑101 (MQ=255)
                                                        aTCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                            >  1:3714748/1‑101 (MQ=255)
                                                        aTCTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.c                            >  1:2816221/1‑101 (MQ=255)
                                                          cTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                          >  2:2903734/1‑101 (MQ=255)
                                                          cTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                          >  1:2745300/1‑101 (MQ=255)
                                                          cTTGACG.AAAAAGG.GGTTGGTGTCGGTCTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGG.ccc                          >  1:560467/1‑101 (MQ=255)
                                                                       aggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGcc                >  2:2260576/11‑101 (MQ=255)
                                                                        ggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa               >  1:3099236/10‑101 (MQ=255)
                                                                        ggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa               <  2:4552239/92‑1 (MQ=255)
                                                                        ggggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCa               >  2:2974024/10‑101 (MQ=255)
                                                                         gggttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAg              <  1:1105261/93‑1 (MQ=255)
                                                                           gttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt           >  1:1777600/7‑101 (MQ=255)
                                                                           gttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tt           >  2:2360611/7‑101 (MQ=255)
                                                                            ttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt          <  2:3054860/96‑2 (MQ=255)
                                                                            ttggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.ttt          >  1:1512037/6‑100 (MQ=255)
                                                                             tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg         <  2:2795825/97‑3 (MQ=255)
                                                                             tggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttg         >  1:2087748/5‑99 (MQ=255)
                                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc        >  1:4423866/4‑98 (MQ=255)
                                                                              ggtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgc        <  1:891673/98‑4 (MQ=255)
                                                                               gtgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcg       >  1:2777074/3‑97 (MQ=255)
                                                                                tgtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAG.tttgcgc      >  2:3214294/2‑96 (MQ=255)
                                                                                 gtcggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGca      <  2:1781800/101‑1 (MQ=255)
                                                                                   cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc    >  2:3876177/1‑101 (MQ=255)
                                                                                   cggtcTTGGTGAT.CAGACAGGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatc    >  2:1453255/1‑101 (MQ=255)
                                                                                     gtcgtggggaa.cagacagGCCCCGGTTTCGGCCGAAGCCGCCCCGGCCAGCGCGGAAAACGCCAGGGCAGAGGCCCCCAACAGGGCCAGTTTGCGcatcat  <  2:3418838/91‑1 (MQ=255)
                                                                                              ||                                                                                           
CATAGGTCGAGGGCACCAGCCCCATCTCGGTCGCCTTTGCCGAAGCACCTTCCTTCATCTTGACG.AAAA..G.GGTTG..GTCGGTCTT.GTGTA.CAGACAGGCCCCGGTTTCGGCCG.AGCCGCCCCGGCCAGCGCGGAAAACGCCA.GGCAGAGG.CCCCAACAGGGCCAG.TTGCGCATCAT  >  NC_014034/2177202‑2177376

Base quality scores:  ATCG  < 3 ≤  ATCG  < 22 ≤  ATCG  < 30 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG