Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,209,679 1 bp→GG 100% intergenic (+11/‑260) cobO → / → cobQ1 cob(I)yrinic acid a,c‑diamide adenosyltransferase/cobyric acid synthase CobQ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,209,6781.→G100.0% 189.3 191intergenic (+10/‑261)cobO/cobQ1cob(I)yrinic acid a,c‑diamide adenosyltransferase/cobyric acid synthase CobQ
Reads supporting (aligned to +/- strand):  new base (34/103):  ref base (32/22):  total (66/125)
Fisher's exact test strand distribution p-value = 1.64e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.15e-01
*NC_0140342,209,6790C→G100.0% 141.7 191intergenic (+11/‑260)cobO/cobQ1cob(I)yrinic acid a,c‑diamide adenosyltransferase/cobyric acid synthase CobQ
Reads supporting (aligned to +/- strand):  new base (35/103):  ref base (31/22):  total (66/125)
Fisher's exact test strand distribution p-value = 3.34e-05
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 4.02e-01

Only 100 of 224 total aligned reads displayed.

ATCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAAG.GC.C..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACAGGGA  >  NC_014034/2209579‑2209780
                                                                                                      ||||                                                                                                       
aTCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..g                                                                                                         <  2:2976908/101‑1 (MQ=255)
 tCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..gg                                                                                                        <  1:1721291/101‑1 (MQ=255)
  cGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..ggg                                                                                                       >  2:2043604/1‑101 (MQ=255)
  cGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGGCGAGTTCTGATCTC.AAG.GC.C..ggg                                                                                                       >  2:1223086/1‑101 (MQ=255)
   gAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CC.C..ggg                                                                                                       >  1:2464606/1‑101 (MQ=255)
   gAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..gggg                                                                                                      >  2:3860814/1‑100 (MQ=255)
      gcggcCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..GGgggcg                                                                                                   <  1:708803/101‑2 (MQ=255)
         gcCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..gggggggctg                                                                                                >  1:4347419/1‑94 (MQ=255)
           cGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.GC.G..gggggggtgcc                                                                                               >  2:1014250/1‑93 (MQ=255)
           cGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.C..GGgggcgctgcc                                                                                              >  2:2210080/1‑95 (MQ=255)
             aTCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGcccc                                                                                             <  2:308196/101‑1 (MQ=255)
              tCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGccccc                                                                                            <  2:3171728/101‑1 (MQ=255)
               cTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCg                                                                                           >  2:1556708/1‑101 (MQ=255)
               cTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCg                                                                                           >  1:3059756/1‑101 (MQ=255)
                 aGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGAATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGa                                                                                         >  2:2751182/1‑101 (MQ=255)
                  gTGACCGAGATGGGTCAGGTGGAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGAc                                                                                        >  1:3492233/1‑101 (MQ=255)
                  gTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGCCCCCGGAc                                                                                        <  1:1784342/101‑1 (MQ=255)
                   tGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGAcc                                                                                       <  2:1433237/101‑1 (MQ=255)
                   tGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGCCCCCGGAcc                                                                                       <  1:3761196/101‑1 (MQ=255)
                        gagaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGGGC.TGCCCCCGGGCCCCC.gg                                                                                 >  1:259208/1‑101 (MQ=255)
                         agaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGCCCCCC.ggg                                                                                >  1:3634938/1‑101 (MQ=255)
                         agaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.ggg                                                                                >  2:4688471/1‑101 (MQ=38)
                         agaTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.ggg                                                                                >  2:492609/1‑101 (MQ=38)
                           aTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGat                                                                              <  1:452731/101‑1 (MQ=38)
                           aTGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGat                                                                              <  2:511975/101‑1 (MQ=38)
                            tGGGTCAGGTGAAGCTCCCGTTCCGGGCAGGCATCAAGGGCCAGCGGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGata                                                                             <  2:580643/101‑1 (MQ=37)
                            tGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGata                                                                             <  2:2386380/101‑1 (MQ=38)
                            tGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGata                                                                             >  2:618590/1‑101 (MQ=38)
                            tGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGata                                                                             <  1:1556809/101‑1 (MQ=38)
                              ggTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATAtt                                                                           <  2:4165022/101‑1 (MQ=38)
                               gTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATAttt                                                                          <  1:1876435/101‑1 (MQ=38)
                                    gTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGc                                                                     <  2:3428055/101‑1 (MQ=37)
                                     tGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCa                                                                    <  1:1263577/101‑1 (MQ=37)
                                     tGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCa                                                                    <  1:3861056/101‑1 (MQ=37)
                                      gAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCaa                                                                   <  2:3789469/101‑1 (MQ=37)
                                       aaGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAg                                                                  <  2:3233985/101‑1 (MQ=25)
                                       aaGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAg                                                                  <  1:4435736/101‑1 (MQ=25)
                                         gCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGAt                                                                <  1:4664629/101‑1 (MQ=25)
                                          cACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATg                                                               <  2:3577425/101‑1 (MQ=25)
                                          cACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATg                                                               <  1:338441/101‑1 (MQ=25)
                                           aCCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGa                                                              <  1:2299928/101‑1 (MQ=25)
                                            cccGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGaa                                                             <  1:2894247/101‑1 (MQ=25)
                                             ccGTTCCGGGCAGGCATCAAGGGCCGGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGaaa                                                            <  2:4667721/101‑1 (MQ=21)
                                             ccGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGaaa                                                            <  1:1293813/101‑1 (MQ=25)
                                              cGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAAc                                                           <  1:4548538/101‑1 (MQ=25)
                                              cGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAAc                                                           <  1:9275/101‑1 (MQ=25)
                                               gTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGGGC.TGCCCCCGGACCCCC.GGGGTATTTGTGGCAAGATGAAACa                                                          >  2:4540640/1‑101 (MQ=25)
                                               gTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACa                                                          <  1:4266995/101‑1 (MQ=25)
                                               gTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACa                                                          <  2:2255125/101‑1 (MQ=25)
                                                ttCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACag                                                         <  1:428511/101‑1 (MQ=25)
                                                ttCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCCGACCCCC.GGGGTATTTGTGGCAAGATGAAACag                                                         >  1:374121/1‑101 (MQ=21)
                                                  ccGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGGGC.TGCCCCCGGACCCCC.GGGGGATTTGTGGGAAGGTGAAAAagag                                                       >  2:2568044/1‑101 (MQ=25)
                                                  ccGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACagag                                                       <  2:1119204/101‑1 (MQ=25)
                                                   cGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGc                                                      <  1:2011310/101‑1 (MQ=25)
                                                    gggCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCg                                                     <  1:1014329/101‑1 (MQ=25)
                                                    gggCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CC.CGGGGGGGG.GGCCCCCGGCCCCCCGGGGATTTTTG.GGCAAGAGGAAA.AGAGCg                                                     >  2:3360349/1‑101 (MQ=255)
                                                      gcaggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGtt                                                   <  1:3420198/101‑1 (MQ=25)
                                                       caggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTa                                                  <  2:3640509/101‑1 (MQ=25)
                                                        aggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.g                                                <  1:2977766/101‑1 (MQ=25)
                                                        aggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.g                                                <  2:710388/101‑1 (MQ=25)
                                                         ggcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.gg                                               <  1:1494121/101‑1 (MQ=25)
                                                          gcaTCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.ggg                                              <  2:731328/101‑1 (MQ=25)
                                                             tCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAtt                                           <  1:577311/101‑1 (MQ=25)
                                                             tCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGGTA.GGGAtt                                           >  1:639935/1‑101 (MQ=21)
                                                              cAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAttt                                          <  1:1188005/101‑1 (MQ=25)
                                                              cAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGAttt                                          <  2:2602739/101‑1 (MQ=25)
                                                                 gggCCAGCCGGGGGTCGAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGt                                       >  1:1405385/1‑101 (MQ=25)
                                                                    gcAGCCGGGGCTCGAGCTCTGCTCTCAAGGCGG.G..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAAc                                    <  2:1692929/100‑1 (MQ=18)
                                                                    ccAGCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAAc                                    <  2:2080013/101‑1 (MQ=25)
                                                                       gCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCAt                                 <  1:4727510/101‑1 (MQ=25)
                                                                       gCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCAt                                 <  1:810563/101‑1 (MQ=25)
                                                                       gCCGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCAt                                 <  1:859020/101‑1 (MQ=25)
                                                                        ccGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATg                                <  1:1818121/101‑1 (MQ=25)
                                                                        ccGGGGGTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATg                                <  1:94465/101‑1 (MQ=25)
                                                                        ccGGGGGTCGAGTTCTGATCTCAAGG.CC.CG.GGGGGCGGGCCCCCGGCCCCCCGGGGATTTTTGGGGC.GGA.GAAACCAAGCGGTAGGGGGTTTGGTAAcatg                                 >  1:3452271/1‑98 (MQ=255)
                                                                          gggggTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGAt                              <  2:4053383/101‑1 (MQ=25)
                                                                           ggggTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATc                             <  2:857420/101‑1 (MQ=25)
                                                                           ggggTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATc                             <  2:1357310/101‑1 (MQ=25)
                                                                            gggTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACGCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCg                            <  2:4249766/101‑1 (MQ=25)
                                                                            gggTCGAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCg                            <  2:1979531/101‑1 (MQ=25)
                                                                               tCGAGTTCTGATCTC.AAG.GC.CGGGGGGGC.TGCCCCCGGGCCCCC.CGGGTATTTGTGGCAAGATGAAAAAGAGCGGTA.GGGATTTGGTAACCACTATCGGGc                         >  2:3616099/1‑101 (MQ=16)
                                                                                cGAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGCCCCCCGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCa                        >  2:2736295/1‑101 (MQ=25)
                                                                                 gAGTTCTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCaa                       <  1:1104832/101‑1 (MQ=25)
                                                                                 gAGTTCTGATCTC.AAG.GC.CGGGGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGGTTTGGTAACCATGATCGGGCaa                       >  2:3803572/1‑101 (MQ=25)
                                                                                   gTTCTGATCTCAAGG.CC.CG.GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGc                     >  2:3748821/1‑101 (MQ=25)
                                                                                      cTGATCTCAAGG.CCGG..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTgg                  <  2:4372782/101‑1 (MQ=25)
                                                                                       tGATCTCAAGG.CC.GG.GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGc                 >  1:2124863/1‑101 (MQ=25)
                                                                                               tcaaggccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAacac            <  1:2145640/91‑1 (MQ=255)
                                                                                               tcaaggccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAAAAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAacac            >  1:1480056/11‑101 (MQ=25)
                                                                                                caaggccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACg           <  2:4424670/92‑1 (MQ=255)
                                                                                                 aaggccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACgg          <  1:2751346/93‑1 (MQ=255)
                                                                                                   ggccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTa        <  1:492660/95‑1 (MQ=255)
                                                                                                    gccgggggCGC.TGCCCCCGGACCCCC.GGGATATATGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTAc       <  1:4069843/96‑1 (MQ=255)
                                                                                                     ccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACa      <  1:210867/97‑1 (MQ=255)
                                                                                                     ccgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACa      <  2:4322366/97‑1 (MQ=255)
                                                                                                      cgggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACag     <  2:3543333/98‑1 (MQ=255)
                                                                                                       gggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACagg    >  1:3894634/3‑101 (MQ=255)
                                                                                                       aggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACagg    >  2:1856483/3‑101 (MQ=255)
                                                                                                        ggggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.TGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACaggg   <  1:3170161/100‑1 (MQ=255)
                                                                                                         gggCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACaggga  <  1:3090650/101‑1 (MQ=255)
                                                                                                      ||||                                                                                                       
ATCGAGGCGGCCGATCTAGTGACCGAGATGGGTCAGGTGAAGCACCCGTTCCGGGCAGGCATCAAGGGCCAGCCGGGGGTCGAGTTCTGATCTCAAAG.GC.C..GGGCGC.TGCCCCCGGACCCCC.GGGATATTTGTGGCAAGATGAAACAGAGCGTTA.GGGATTTGGTAACCATGATCGGGCAAGCTGGCAACACGGTACAGGGA  >  NC_014034/2209579‑2209780

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG