Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,253,265 Δ1 bp 6.9% coding (239/2421 nt) copA2 ← copper‑transporting P‑type ATPase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,253,2650G→. 6.9% 22.0 ‑0.7 ‑0.1131coding (239/2421 nt)copA2copper‑transporting P‑type ATPase
Reads supporting (aligned to +/- strand):  new base (6/3):  ref base (63/59):  total (69/62)
Fisher's exact test strand distribution p-value = 4.98e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 8.27e-01

Only 100 of 190 total aligned reads displayed.

CGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTC  >  NC_014034/1253165‑1253365
                                                                                                     |                                                                                                    
cGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTccgcc.g                                                                                                      <  1:2738906/101‑1 (MQ=255)
cGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTccgcc.g                                                                                                      <  2:4630684/101‑1 (MQ=255)
cGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTccgcc.g                                                                                                      >  1:972028/1‑101 (MQ=255)
cGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTccgcc.g                                                                                                      >  1:3925856/1‑101 (MQ=255)
   gCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggg                                                                                                   <  2:1075432/101‑1 (MQ=255)
        gcgcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.ggggggggg                                                                                              <  2:382016/101‑1 (MQ=255)
          gcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGc                                                                                            <  1:1044405/101‑1 (MQ=255)
          gcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.ggggggggggg                                                                                            >  1:861623/1‑100 (MQ=255)
          gcATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGc                                                                                            >  2:3419123/1‑101 (MQ=255)
           cATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.gggggggggggg                                                                                           >  1:3755443/1‑99 (MQ=255)
            aTAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGt                                                                                          <  2:16503/101‑1 (MQ=255)
             tgggTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCCCC.GGGGGGGGGGCGTc                                                                                         <  2:2145369/99‑1 (MQ=255)
             tAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCggc                                                                                         >  1:292263/1‑99 (MQ=255)
             tAGGGGGCGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTc                                                                                         <  1:712419/101‑1 (MQ=255)
              aGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCg                                                                                        >  2:1551872/1‑101 (MQ=255)
              aGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCg                                                                                        >  2:183438/1‑101 (MQ=255)
              aGGTGACGCGGGCGCTGCCCGCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCg                                                                                        <  1:3351003/101‑1 (MQ=255)
               ggTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGa                                                                                       >  1:1318704/1‑101 (MQ=255)
               ggTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGa                                                                                       >  1:2512900/1‑101 (MQ=255)
                gTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCGaa                                                                                      >  1:1438519/1‑101 (MQ=255)
                gTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGaa                                                                                      >  1:4583749/1‑101 (MQ=255)
                  gACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGc                                                                                    <  1:4950379/101‑1 (MQ=255)
                   aCGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGATCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGcc                                                                                   >  1:300146/1‑101 (MQ=255)
                     gcgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCa                                                                                 <  2:4711896/101‑1 (MQ=255)
                      cgGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAg                                                                                <  1:474552/101‑1 (MQ=255)
                        gggccgtcccTCGGGCAAAAGGAGGACCCGGAGCCCCCCGGGGGTCGCCTGCAGCGCGGGCACGTCCCCGTCCCCC.GGGGGGGGGGCGTCGAAGCCCAGAc                                                                              <  1:5009819/94‑1 (MQ=255)
                          cgcTGCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCCCC.GGGGGGGGGGCGTCGAAGCCCAGACAg                                                                            <  1:1253887/101‑1 (MQ=255)
                            cTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCGAAGCCCAGACAGGt                                                                          >  2:1893523/1‑101 (MQ=255)
                             ggccccggggCAAAAGCGGGCCCGGGGCCCCCCCGGGCGCCCCCTGCAGCGCGGCCCCGCCCCGGCCCCCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGtc                                                                         <  2:1004616/94‑1 (MQ=255)
                              gCCCTCGGGCAAAAGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGtct                                                                        <  1:4684493/101‑1 (MQ=255)
                              gCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGtct                                                                        >  1:2790157/1‑101 (MQ=255)
                                    gggCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGGCGAAGCCCAGACAGGTctcggccc                                                                  >  2:3050357/1‑100 (MQ=255)
                                      gCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTctcggcctcgg                                                               <  1:4659416/101‑1 (MQ=255)
                                       cAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGCCGAAGCCCAGGCCGGTctccgcctcgg                                                               >  2:330196/1‑101 (MQ=255)
                                        aaaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCCGGGGGGGGGGGCGTCGAAGCCAAGACAGGGctcggcctcgg                                                               >  1:2972597/1‑101 (MQ=255)
                                        aaaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCa                                                             >  2:2330426/1‑101 (MQ=255)
                                        aaaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGTCGAAGCCCAGACAGGCCTCGGCCTCGGCa                                                             >  2:4100216/1‑101 (MQ=255)
                                         aaaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGG.GGCGAAGGCCCAACAGGGtccggccccgggca                                                            >  2:3941226/1‑98 (MQ=255)
                                          aaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCGAAGCCCAGACAGGCCTCGGCCTCGGCaa                                                            >  1:3293685/1‑101 (MQ=255)
                                          aaGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCaa                                                            >  1:1829292/1‑101 (MQ=255)
                                           aGCAGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCGAGACAGGTCTCGGCCTCGGCAAt                                                           <  2:4520909/101‑1 (MQ=255)
                                             cAGGACCGAGGGCACCCCGGGAGTCGCCTGCAGGGGGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCg                                                         <  2:279351/101‑1 (MQ=255)
                                             cAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCg                                                         >  1:2287309/1‑101 (MQ=255)
                                             cAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCg                                                         >  2:4287075/1‑101 (MQ=255)
                                              aGGACCGAGAGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCgg                                                        <  2:4043607/101‑1 (MQ=255)
                                              aGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTGGAAGCCCAAACAGGTCGCGGCCTCGGCAATCgg                                                        >  1:758592/1‑101 (MQ=255)
                                              aGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCgg                                                        <  2:807932/101‑1 (MQ=255)
                                              aGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGCCCCGGCCTCGGCAACCgg                                                        >  2:3153568/1‑101 (MQ=255)
                                                gACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC..GGGGGGGGGCGGCGAAGCCCAGACAGGGCTCGGCCTCTGGAATGGGATa                                                     >  1:1567438/1‑101 (MQ=255)
                                                 gccGAGGGCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATa                                                     <  2:3659574/100‑1 (MQ=255)
                                                 aCCGAGAGCGCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCGGTCCGCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATa                                                     <  2:5030571/101‑1 (MQ=255)
                                                   cgggggccccccGGGCGCCCCCTGCGGCCGGGACCCGCCCCTGCCCCCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgc                                                   <  1:4841003/93‑1 (MQ=255)
                                                    gagaGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcc                                                  <  1:1686727/101‑1 (MQ=255)
                                                    gagaGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCCCGGCAAAGGGATAacc                                                  >  1:4060470/1‑101 (MQ=255)
                                                     agagCCCCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                                 <  1:4456034/101‑1 (MQ=255)
                                                     agagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGGGTCGAAGCCCAGACCGGTCTCGGCCTCGGCAATGGGATAgccc                                                 >  1:3560270/1‑101 (MQ=255)
                                                     agagCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgccc                                                 >  2:2883615/1‑101 (MQ=255)
                                                         cgatctgggCGTCGCCTGCAGCGCGGACCCGACCCTGTCCCCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgccc                                             <  2:1607619/95‑1 (MQ=255)
                                                          cccccGGGCGTCGCCTGCAGCGCGGACACGCCCCTGTCCGCCGGGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAgcccgccc                                             <  1:573481/100‑1 (MQ=255)
                                                          cccccGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGcccgcccg                                            <  1:2868842/100‑1 (MQ=255)
                                                               gggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCa                                       >  2:1317798/1‑101 (MQ=255)
                                                               gggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCa                                       >  2:1785022/1‑101 (MQ=255)
                                                               gggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCa                                       <  1:2082000/101‑1 (MQ=255)
                                                                ggCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGCCAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAg                                      >  1:2569797/1‑101 (MQ=255)
                                                                  cGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCCGCAATCGCATAGCCCGCCCCCGcccggc                                    >  1:1073188/1‑97 (MQ=255)
                                                                   gTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAgcgc                                   >  2:3214857/1‑101 (MQ=255)
                                                                     cGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCCCCCGCGCCAGCGCCt                                 >  2:2644725/1‑101 (MQ=255)
                                                                       ccTGCGGCGCGGACCCGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCg                               <  1:1893660/101‑1 (MQ=255)
                                                                       ccTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCACCCGCGCCAGCGCCTCg                               >  1:1099086/1‑101 (MQ=255)
                                                                          gCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                            >  1:2420702/1‑101 (MQ=255)
                                                                          gCAGCGCGGAAACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGcc                            <  1:1653147/101‑1 (MQ=255)
                                                                            ggcggGGACACGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCATACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAg                          <  2:292210/100‑1 (MQ=255)
                                                                             gcgcGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCCGCGCCCCGGCCAgg                         >  2:1077166/1‑101 (MQ=255)
                                                                              cgcgGACCCGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAggg                        <  2:191185/101‑1 (MQ=255)
                                                                               gcgGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCCCGCCAGCGCCCCGGCCCGGGt                       >  2:147491/1‑101 (MQ=255)
                                                                                gggaccgcccccTGTCCTCG.GGGGGGGGGGCGTCGAAGCCCAGACAGGGCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGtt                      <  2:2421523/92‑1 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGttt                     >  1:4005277/1‑101 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGttt                     >  1:4284680/1‑101 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGttt                     >  1:4670884/1‑101 (MQ=255)
                                                                                 ggACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGttt                     >  2:2907825/1‑101 (MQ=255)
                                                                                  gccccgccccTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTc                    <  2:199141/94‑1 (MQ=255)
                                                                                  gACCCGCCCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTc                    <  1:3050595/101‑1 (MQ=255)
                                                                                   acacGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGGTTcc                   >  2:678480/1‑101 (MQ=255)
                                                                                    cacGCCCCGGTCCCCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccg                  <  2:2276785/101‑1 (MQ=255)
                                                                                    cacGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccg                  <  1:369496/101‑1 (MQ=255)
                                                                                    cacGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATACCCCGCCCCCGCCAGCGCCTCGGCCAGGGTTTccc                  >  1:1743789/1‑100 (MQ=255)
                                                                                      ccgcccgggccgcc.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcc                <  2:4454308/92‑1 (MQ=255)
                                                                                      cGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcc                >  2:2114972/1‑101 (MQ=255)
                                                                                      cGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCCCGGCCAGGGTTTccgcc                >  2:3520452/1‑101 (MQ=255)
                                                                                       gACCCTGTCCGCC.GGGGGGGGGGCGTCGGAGCCCCGCCGAATATCGGCCTCAACAAACGGAAAGCCCGCACCCGCCCGCGCCATGTCCAGGGTTTccgccc               >  1:4184675/1‑101 (MQ=255)
                                                                                        ccccggcccgcg.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg              <  2:2701094/94‑1 (MQ=255)
                                                                                        ccccTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTccgcccg              <  2:4060167/100‑1 (MQ=255)
                                                                                           cTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCgcgc           >  2:2197794/1‑101 (MQ=255)
                                                                                             gTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCCCCg         >  1:1509117/1‑101 (MQ=255)
                                                                                              ccggtctggggggggggCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCg         <  2:1207680/94‑1 (MQ=255)
                                                                                               ccgcg.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCggg       <  1:1610319/101‑1 (MQ=255)
                                                                                                  cc.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGGTTCCGCCCGCGCCGGGGcc    >  1:674185/1‑101 (MQ=255)
                                                                                                   c.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCt   >  1:2068409/1‑101 (MQ=255)
                                                                                                   c.gGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCt   >  1:692436/1‑101 (MQ=255)
                                                                                                     ggggggggggCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCCCGGCCAGGGTTTCCGCCCCCGCCgagatcgg  >  1:1207797/1‑94 (MQ=255)
                                                                                                     |                                                                                                    
CGTGCCCTGCGCATAGGTGACGCGGGCGCTGCCCTCGGGCAAAAGCAGGACCGAGAGCACCCCGGGCGTCGCCTGCAGCGCGGACACGACCCTGTCCGCC.GGGGGGGGGGCGTCGAAGCCCAGACAGGTCTCGGCCTCGGCAATCGGATAGCCCGCCCGCGCCAGCGCCTCGGCCAGGGTTTCCGCCCGCGCCGGGGCCTC  >  NC_014034/1253165‑1253365

Base quality scores:  ATCG  < 3 ≤  ATCG  < 12 ≤  ATCG  < 20 ≤  ATCG  < 31 ≤  ATCG  < 40 ≤  ATCG