Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,395,795 Δ1 bp 2.9% intergenic (+607/‑324) RCAP_rcc01306 → / → RCAP_rcc01307 Cas2 family CRISPR‑associated protein/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,395,7950C→. 2.9% 14.8 ‑0.7 0.0271intergenic (+607/‑324)RCAP_rcc01306/RCAP_rcc01307Cas2 family CRISPR‑associated protein/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (3/5):  ref base (135/128):  total (138/133)
Fisher's exact test strand distribution p-value = 4.94e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 317 total aligned reads displayed.

CGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGC  >  NC_014034/1395695‑1395895
                                                                                                             |                                                                                                       
  tggTCGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         <  2:1387470/99‑1 (MQ=2)
   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                                                        >  2:3711699/2‑101 (MQ=2)
   ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcc                                                                                                        <  1:1258730/100‑1 (MQ=2)
   aGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                                                         <  1:955020/101‑1 (MQ=2)
    gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                                                     >  1:1314846/1‑101 (MQ=255)
    gTCGCTCCCCCCGCGGGGGAGTGGATCGAAACT.GGTATG.GATCTG..GCGTAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgc                                                                                                     <  1:2830236/101‑1 (MQ=255)
    gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAGAca                                                                                                        <  1:1896147/101‑2 (MQ=2)
     tCGCTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcac                                                                                                       <  1:2535894/101‑3 (MQ=2)
     gcGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.ATGACCAAAT..CCGCAATCGG..CCGGGCT.GGATCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCta                                                                                                      <  1:4264405/100‑2 (MQ=2)
      cGCTCCCCCCGCGGGGGTGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                                                     <  2:4539446/101‑3 (MQ=2)
      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                                                     <  2:4289950/101‑3 (MQ=2)
      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtat                                                                                                     <  1:4949381/101‑3 (MQ=2)
      cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGGCT.G..TCCTCGCTCCCCCCGCGGGGGCGGGGCTCGAAAGCTgtg.g                                                                                                  >  2:4184789/1‑101 (MQ=25)
       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                                                    <  1:155601/101‑4 (MQ=2)
       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatg                                                                                                    <  2:5007659/101‑4 (MQ=2)
       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gc                                                                                                 <  1:1744697/101‑1 (MQ=255)
       gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gc                                                                                                 <  1:618059/101‑1 (MQ=255)
        ccccccccGGGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCCCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                                                                    <  1:2651617/99‑6 (MQ=2)
        cTCCCCCCGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccg                                                                                                    >  2:2897307/1‑96 (MQ=2)
        cTCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatga                                                                                                   <  1:4987852/101‑5 (MQ=2)
        cTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gcg                                                                                                >  1:2267527/1‑101 (MQ=255)
         tCCCCCCGCGGGGGCGTGGATCGAAACT.TG.AT..GACCAGATCCGCAATCGG..CCGGGCTCGA.TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCtatgag                                                                                                  >  1:2439499/1‑96 (MQ=2)
         tCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTgcg.gcgg                                                                                               >  2:1599849/1‑100 (MQ=255)
         cccccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccgg                                                                                                   <  2:2086059/100‑7 (MQ=0)
          ccccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggc                                                                                                  <  2:4338879/101‑8 (MQ=0)
          ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                                                              >  1:84409/1‑101 (MQ=255)
          ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                                                              <  2:4576296/101‑1 (MQ=255)
          ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTg                                                                                              >  1:3330049/1‑101 (MQ=255)
          ccccccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACCTGCG.GCGTg                                                                                              >  1:2194961/1‑101 (MQ=255)
           cccccGCGGGGGCGTGGATCGAAACTCGGCAAG.GACATC..GCCGATCTGGGTAAGGCCG.G..TGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcacccggcg                                                                                                 >  2:3771739/1‑93 (MQ=2)
             cccGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGGCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGt                                                                                           <  1:4623008/101‑1 (MQ=255)
                gcgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGc                                                                                        <  2:3127920/101‑1 (MQ=255)
                 cgGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGcc                                                                                       <  1:2679592/101‑1 (MQ=255)
                  gggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCa                                                                                      >  1:2319675/1‑101 (MQ=255)
                   ggggCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAg                                                                                     >  2:1964466/1‑101 (MQ=255)
                      gCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGa                                                                                  <  2:2694452/101‑1 (MQ=255)
                       cGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGAt                                                                                 <  1:4326667/101‑1 (MQ=255)
                        gTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATa                                                                                >  2:2752649/1‑101 (MQ=255)
                         tGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTTCG.GCGTGAGTTGCCAGTGATAg                                                                               <  2:3948830/101‑1 (MQ=255)
                         gggATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCT.CCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGa                                                                              <  1:3491890/100‑1 (MQ=255)
                           gATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGACAGA.c                                                                            <  2:4273559/101‑1 (MQ=255)
                            aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.Cg                                                                           <  1:444478/101‑1 (MQ=255)
                            aTCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.Cg                                                                           >  1:2985520/1‑101 (MQ=255)
                              cGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGcc                                                                         <  1:2080246/101‑1 (MQ=255)
                                aaaCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGt                                                                       >  2:3326507/1‑101 (MQ=255)
                                  aCT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTgg                                                                     >  1:1677645/1‑101 (MQ=255)
                                   cT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGa                                                                    >  2:2219080/1‑101 (MQ=255)
                                    t.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.c                                                                  <  1:3282583/101‑1 (MQ=255)
                                       gTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGt                                                                <  2:3621307/101‑1 (MQ=255)
                                        tATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTc                                                               >  1:3443522/1‑101 (MQ=255)
                                         aTG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCg                                                              <  2:2016822/101‑1 (MQ=255)
                                           g.gATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCt                                                            >  1:3807805/1‑101 (MQ=255)
                                           g.gATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTCATAGA.CGCCGTGGA.CGTCGCt                                                            >  2:3399631/1‑101 (MQ=255)
                                              aTCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcc                                                          >  2:2225371/1‑101 (MQ=255)
                                                 tG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTccccc                                                       <  2:3862515/101‑1 (MQ=255)
                                                  g..gCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                      <  1:3050235/101‑1 (MQ=255)
                                                  g..gCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTcccccc                                                      >  2:4673375/1‑101 (MQ=255)
                                                        cAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgg                                                  >  1:4738905/1‑101 (MQ=255)
                                                          aaCGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTAAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCgggg                                                <  1:4029958/101‑1 (MQ=255)
                                                            cGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                              >  1:1614343/1‑101 (MQ=255)
                                                            cGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGc                                              <  1:1008604/101‑1 (MQ=255)
                                                              g..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGt                                            <  2:4889613/101‑1 (MQ=255)
                                                                 aaaGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                           >  2:4363281/1‑101 (MQ=255)
                                                                 aaaGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTg                                           <  1:2752851/101‑1 (MQ=255)
                                                                    ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCgg                                            >  2:4559378/10‑100 (MQ=0)
                                                                    ggacccgcccgtcgCTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCGCGGGGGCGt                                            <  2:3042928/92‑1 (MQ=0)
                                                                    gTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTgggt                                        >  1:4543037/1‑99 (MQ=255)
                                                                     tCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATc                                       >  2:647316/1‑101 (MQ=255)
                                                                      cT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGGGGATCg                                      >  2:2495121/1‑101 (MQ=255)
                                                                      cT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCCGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCg                                      >  2:72287/1‑101 (MQ=255)
                                                                         g..tcgtcGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                    >  2:21099/1‑101 (MQ=255)
                                                                            tcgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                   <  2:4783071/101‑1 (MQ=255)
                                                                             cgtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                  >  2:4473048/1‑101 (MQ=255)
                                                                              gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                 >  2:1176397/1‑101 (MQ=255)
                                                                              gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                 >  2:4961735/1‑101 (MQ=255)
                                                                              gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                 <  1:386729/101‑1 (MQ=255)
                                                                              gtcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCACTGAAAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGGGGATCGAAACt                                 >  2:2331364/1‑101 (MQ=255)
                                                                               tcgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTg                                <  1:5038160/101‑1 (MQ=255)
                                                                                cgCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTGGCTCCCCCCGCGGGGGCGTGGATCGAAACAgc                               >  2:1535704/1‑101 (MQ=255)
                                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTgcg                              >  2:1627830/1‑101 (MQ=255)
                                                                                 gCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGGGGGTCGAAACTgcg                              >  1:3413641/1‑101 (MQ=255)
                                                                                  cTCCCCCCGCGGGGGCGTGGATCGAAA.CTGCGTGCGCGCCATG.AATTCATCGAGCGCCATCGAGCGTCGCTCCCCCCCCGGGGGCGTGGAtgggaccacca                              >  1:1381833/1‑91 (MQ=0)
                                                                                   tCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgcc                            >  2:893033/1‑101 (MQ=255)
                                                                                    ccccccGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGcgccg                           <  1:2026801/101‑1 (MQ=255)
                                                                                          gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                     <  1:291210/101‑1 (MQ=255)
                                                                                          gcgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATc                     <  2:2426807/101‑1 (MQ=255)
                                                                                           cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                    <  1:273357/101‑1 (MQ=255)
                                                                                           cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                    <  2:352847/101‑1 (MQ=255)
                                                                                           cgGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCg                    <  1:1080232/101‑1 (MQ=255)
                                                                                            gggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                   >  1:4153293/1‑101 (MQ=255)
                                                                                            gggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGc                   >  1:4826520/1‑101 (MQ=255)
                                                                                             ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                  >  1:3685927/1‑101 (MQ=255)
                                                                                             ggggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCa                  >  2:2291396/1‑101 (MQ=255)
                                                                                               ggCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATc                >  1:1840181/1‑101 (MQ=255)
                                                                                                gCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATcc               <  1:1144161/101‑1 (MQ=255)
                                                                                                  gTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCgg             >  1:2048233/1‑101 (MQ=255)
                                                                                                    ggATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTc           >  2:1814082/1‑101 (MQ=255)
                                                                                                     gATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCg          <  1:362210/101‑1 (MQ=255)
                                                                                                       tCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAg        <  2:4036782/101‑1 (MQ=255)
                                                                                                        cGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAgc       >  1:3131049/1‑101 (MQ=255)
                                                                                                             |                                                                                                       
CGCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GGTATG.GATCTG..GCGCAAACGG..AAAGTCT.G..TCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCTGCG.GCGTGAGTTGCCAGTGATAGA.CGCCGTGGA.CGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTGCGCCGCCTATCGCATCCGGTCGAGCGCCGC  >  NC_014034/1395695‑1395895

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG