Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,396,936 Δ1 bp 10.6% coding (818/1578 nt) RCAP_rcc01307 → hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,396,9360G→.10.6% 137.6 ‑0.8 ‑0.3426coding (818/1578 nt)RCAP_rcc01307hypothetical protein
Reads supporting (aligned to +/- strand):  new base (26/19):  ref base (192/186):  total (220/206)
Fisher's exact test strand distribution p-value = 4.31e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.42e-01

Only 100 of 457 total aligned reads displayed.

GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTC  >  NC_014034/1396836‑1397036
                                                                                                     |                                                                                                                  
 tCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.Gc                                                                                                                   >  1:3512839/1‑101 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                                 <  2:4744704/101‑1 (MQ=255)
   gCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCt                                                                                                                 >  1:573707/1‑101 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                               <  1:1041585/101‑1 (MQ=255)
    cTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.g                                                                                                               <  2:813462/101‑1 (MQ=255)
     cccccccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.gc                                                                                                              <  2:3217378/100‑1 (MQ=255)
          ccGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGGAACA.GCCT.GCGCG.G..c                                                                                                      >  2:300366/1‑101 (MQ=255)
              gggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.a                                                                                                >  1:2959220/1‑101 (MQ=255)
               ggggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAANA.GCCT.GCGCG.G..C.CGC.A..t                                                                                             >  2:2260101/1‑101 (MQ=255)
                gggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..Tc                                                                                            <  2:2157452/101‑1 (MQ=255)
                 ggCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCg                                                                                           <  1:1830431/101‑1 (MQ=255)
                   cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.c                                                                                        >  2:439317/1‑101 (MQ=255)
                   cGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.c                                                                                        >  2:3322895/1‑101 (MQ=255)
                    gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.Ct                                                                                       >  2:2364252/1‑101 (MQ=255)
                    gTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.Ct                                                                                       <  1:2436319/101‑1 (MQ=255)
                     tGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.Ct.t                                                                                     >  2:958840/1‑101 (MQ=255)
                      ggATCGAACCCGGTCCAGAGATAGGCCGCGNGGTCCCCCTAAGACGTCTCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.Tc                                                                                    <  1:1424647/101‑1 (MQ=37)
                      ggATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.Tc                                                                                    >  2:2689207/1‑101 (MQ=255)
                       gATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.Tca                                                                                   >  1:3671096/1‑100 (MQ=255)
                        aTCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCg                                                                                  >  2:4124382/1‑101 (MQ=255)
                          cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGc.c                                                                               >  1:4975406/1‑101 (MQ=255)
                          cGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGc.c                                                                               <  1:3593500/101‑1 (MQ=255)
                           gAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGc.cc                                                                              >  1:1732799/1‑100 (MQ=255)
                            aaaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTg                                                                             >  2:1988327/1‑101 (MQ=255)
                             aaCCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..c                                                                          >  1:4145564/1‑101 (MQ=255)
                                  gTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CCG..CGTG.Gt                                                                    >  1:2327877/1‑101 (MQ=255)
                                    ccAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCg                                                                  >  1:440449/1‑101 (MQ=255)
                                      agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCt                                                                >  1:1299497/1‑101 (MQ=255)
                                      agagATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCt                                                                <  2:3512569/101‑1 (MQ=255)
                                       gagaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTc                                                               >  2:1116184/1‑101 (MQ=255)
                                        agaTAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcc                                                              >  2:1233636/1‑101 (MQ=255)
                                            aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                          >  2:3407247/1‑101 (MQ=255)
                                            aGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTcccccc                                                          <  2:5028414/101‑1 (MQ=255)
                                             gACCGCGCGGACCTCCTCAGACGTCGCTCCCGCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCcg                                                         >  1:4485513/1‑101 (MQ=255)
                                             gACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GGCGCTCCCCCcg                                                         >  2:4440185/1‑101 (MQ=255)
                                                      gccctcctCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                <  2:3633955/99‑1 (MQ=255)
                                                      gACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGt                                                <  2:1809747/101‑1 (MQ=255)
                                                         tcgcggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTgg                                              <  2:1455824/92‑1 (MQ=0)
                                                         ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                              >  1:4002526/11‑101 (MQ=9)
                                                         ggtcaacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTgg                                              <  1:1474956/91‑1 (MQ=9)
                                                          tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGAt                                            >  1:1894328/1‑101 (MQ=255)
                                                          tcctcAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCCCGGGGGCCTgggt                                            >  1:4765541/1‑99 (MQ=255)
                                                          ccctcAGACGTCGCCCCCCCCGGGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGC.GGGGCGTGGATc                                           <  2:41905/100‑1 (MQ=38)
                                                           tggccccgggcGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:29207/91‑1 (MQ=2)
                                                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           >  1:1945509/9‑101 (MQ=2)
                                                           tggccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  1:3217404/93‑1 (MQ=2)
                                                           tcaccactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGAt                                            <  1:1344756/93‑1 (MQ=9)
                                                            ctcAGACGTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCATGGATCg                                          <  2:3920075/101‑1 (MQ=255)
                                                            cggttgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATc                                           <  2:4939102/95‑1 (MQ=2)
                                                             ggttgcGTCGCGCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          <  2:1666920/96‑1 (MQ=2)
                                                             ccAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGa                                         <  1:2218559/100‑1 (MQ=255)
                                                             aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCg                                          >  1:4290267/7‑101 (MQ=9)
                                                             aacactgTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCCCCCCCCGCGGGGGGGGGGGTCg                                          >  1:3902051/7‑101 (MQ=2)
                                                              ccccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:4591282/96‑1 (MQ=2)
                                                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaa                                        <  2:3157727/101‑1 (MQ=255)
                                                              cAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGGGGATCGaa                                        >  1:635910/1‑101 (MQ=255)
                                                               cccggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:903933/97‑1 (MQ=2)
                                                                tgcGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GGCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       >  2:290430/3‑101 (MQ=2)
                                                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTTGCTCCCCCCGCGGGGGCGGGGATCGAAAc                                      >  1:747289/1‑101 (MQ=255)
                                                                gACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                      <  1:3771865/101‑1 (MQ=255)
                                                                 cggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct                                     >  2:3058242/3‑100 (MQ=2)
                                                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActa                                    <  1:2001456/100‑3 (MQ=2)
                                                                  cGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                       <  1:2838087/101‑1 (MQ=2)
                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccc                                    <  2:1090364/101‑3 (MQ=2)
                                                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActc                                    <  1:989258/101‑3 (MQ=9)
                                                                   gTCGCCCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATc                                   <  2:4842033/101‑1 (MQ=255)
                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACa                                     <  2:3735872/101‑1 (MQ=2)
                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                  >  1:4860951/1‑101 (MQ=255)
                                                                    tCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCa                                  <  1:2667094/101‑1 (MQ=255)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACac                                    <  2:1704308/101‑2 (MQ=2)
                                                                     cGCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGTGCGTGGATCGAAActaaac                                 >  2:4009691/1‑96 (MQ=2)
                                                                     cGCCCCCCCCGCGGGGGCGTGGATCGAAACT.TGAT.GCCCA.GATC.CGCAA..TCGG.C...CGGG.CTC..GATC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcctat                                  <  2:2876833/101‑5 (MQ=0)
                                                                      gCTCCCCCCGCGGGGGCGTGGATTGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGATCCCCCCGCGGGGGGGTGGTTCGAAACATAAGc                                >  1:4064415/1‑101 (MQ=37)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACacc                                   >  1:4938516/1‑99 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGGACTATGCGC.TGGA.CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAccacacc                                >  1:1638978/1‑95 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAACA..ACT.GACC.....C.GGC.A..ACGG.CC.TATGCGCTGGACGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActacacc                                <  1:2155961/101‑7 (MQ=2)
                                                                      gCTCCCCCCGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActcggc                                 >  1:4218476/1‑96 (MQ=9)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                  <  1:3411648/101‑1 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCa                                  <  1:4542812/101‑1 (MQ=2)
                                                                       cTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCTCCGCGGGGGCGTGGATCGAAACATCAGCt                               <  2:468931/101‑1 (MQ=255)
                                                                        tCCCCCCGCGGGGGCGTGGATCGAAACA.CCCG.GCGCC.G..C.AGC.G..AGTG.CG.TCTGA.ACA..GGAT.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAActatgctgg                              >  2:2807018/1‑93 (MQ=2)
                                                                         ccccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaat                                <  1:313218/101‑3 (MQ=2)
                                                                         ccccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGGGGATCGAAAccccccacc                              >  2:1299302/1‑93 (MQ=2)
                                                                         ccccccGCGGGGGCGTGGATCGAAAC..GCTG.GCGCGCG..C.CGC.G..CCGG.ATCTTTGT.CTG..CGCG.GTCGCTCCCCCCGCGGGGGCGTGGCTCGAAActcggcctc                              >  2:664684/1‑93 (MQ=9)
                                                                          cccccGCGGGGGCGTGGATCGAAACATGGCTATGGCG.G..C.AGT.G..TCAG.GT.T.TTC.CAG..CGGC.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAAcccgccacgc                             >  1:2290327/1‑92 (MQ=2)
                                                                          cccccGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCtcgt                            <  1:1502598/101‑1 (MQ=255)
                                                                           ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGaaaaaccaatgc                              >  2:2444389/1‑92 (MQ=2)
                                                                           ccccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..C.ATG.AGTTCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgc                              <  2:4299223/101‑5 (MQ=0)
                                                                             ccGGGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTcgtcgt                         <  1:682592/101‑1 (MQ=255)
                                                                             ccGCGGGGGCGTGGATCGAAACT.GCGT.GCGCG.C..CATGA.AT.TCAT.CG.AGCGC.CAT..CGAGCGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACACCaatgccg                            <  1:1141240/101‑7 (MQ=0)
                                                                              cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                        <  1:210884/101‑1 (MQ=255)
                                                                              cgcgGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGtt                        <  1:3333269/101‑1 (MQ=255)
                                                                                cgGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGGG.GTCGCTCCCCCCGCGGGGGCGTGGATCGGAACATCAGCTCGTCGTTgg                      >  1:3792349/1‑101 (MQ=255)
                                                                                 gggggCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGt                     <  2:2651635/101‑1 (MQ=255)
                                                                                      gggggATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                <  2:1504348/98‑1 (MQ=255)
                                                                                      ggTGGATAGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGcc                <  2:747199/100‑1 (MQ=255)
                                                                                         ggATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGa             >  2:1399203/1‑101 (MQ=255)
                                                                                                 aCA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTcc     >  2:1913806/1‑101 (MQ=255)
                                                                                                  cA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCg    <  1:2595785/101‑1 (MQ=255)
                                                                                                     gCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTc  <  1:2753155/101‑1 (MQ=255)
                                                                                                     |                                                                                                                  
GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACCGGTCCAGAGATAGACCGCGCGGACCTCCTCAGACGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACA.GCCT.GCGCG.G..C.CGC.A..TCGG.CT.TCCGC.CTG..CGTG.GTCGCTCCCCCCGCGGGGGCGTGGATCGAAACATCAGCTCGTCGTTGGTCAGCCGGACGATGTCCGTC  >  NC_014034/1396836‑1397036

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG