Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 1,397,698 Δ1 bp 2.0% intergenic (+2/+1896) RCAP_rcc01307 → / ← RCAP_rcc01309 hypothetical protein/IS66 family transposase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140341,397,6980A→. 2.0% 6.2 ‑1.8 ‑0.0244intergenic (+2/+1896)RCAP_rcc01307/RCAP_rcc01309hypothetical protein/IS66 family transposase
Reads supporting (aligned to +/- strand):  new base (1/4):  ref base (143/96):  total (144/100)
Fisher's exact test strand distribution p-value = 1.62e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.86e-01

Only 100 of 265 total aligned reads displayed.

CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798
                                                                                                    |                                                                                                    
cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                                      >  2:2554434/1‑101 (MQ=255)
cATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACa                                                                                                      <  1:3554694/101‑1 (MQ=255)
    cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                                  <  1:1229291/101‑1 (MQ=255)
    cgcgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGATCCGGTCTTGTTCTGTTCCGGTAACAGGTc                                                                                                  >  2:2486518/1‑101 (MQ=255)
      cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTNTTCCGGTAACNNGNNNc                                                                                                >  2:1308823/1‑101 (MQ=255)
      cgGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGc                                                                                                <  2:4998902/101‑1 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                               >  2:3451127/1‑101 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCt                                                                                               >  2:3978047/1‑101 (MQ=255)
       ggATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGCCTTGTTCTGTTCCGGCAACAGTTCGCt                                                                                               >  2:2429101/1‑101 (MQ=255)
        gATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTc                                                                                              >  1:2426149/1‑101 (MQ=255)
            ggCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                          <  2:3143598/101‑1 (MQ=255)
            ggCGTGCCCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                          <  2:1036909/101‑1 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                          >  2:2016419/1‑101 (MQ=255)
            ggCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTccccc                                                                                          >  2:2597590/1‑101 (MQ=255)
                ggccccgccccgcCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCcgcg                                                                                      <  2:2458510/98‑1 (MQ=255)
                    ccgccccgccCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCggggg                                                                                  <  1:2050508/101‑3 (MQ=255)
                      gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                                                >  2:408589/1‑101 (MQ=255)
                      gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                                                >  2:1739325/1‑101 (MQ=255)
                      gccccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAATACAGAGTTCCGGCCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGggcg                                                                                >  2:1775060/1‑101 (MQ=255)
                        cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGNGCGTg                                                                              >  1:1000238/1‑101 (MQ=255)
                        cccgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTg                                                                              >  2:651626/1‑101 (MQ=255)
                          cgcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGa                                                                            <  1:3622583/101‑1 (MQ=255)
                           gcccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGAt                                                                           <  1:1783579/101‑1 (MQ=255)
                            cccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATc                                                                          <  1:4609814/101‑1 (MQ=255)
                             ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  2:1992227/101‑1 (MQ=255)
                             ccATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCg                                                                         <  1:3916087/101‑1 (MQ=255)
                                tCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa                                                                      >  2:1693434/1‑101 (MQ=255)
                                 cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                     >  1:4945445/1‑101 (MQ=255)
                                 cGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc                                                                     <  1:2630642/101‑1 (MQ=255)
                                  ggTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACt                                                                    >  2:4346889/1‑101 (MQ=255)
                                   gTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACtt                                                                   <  1:1941827/101‑1 (MQ=255)
                                       cTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCAGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCt                                                               <  2:4157297/101‑1 (MQ=255)
                                             ccGCGGGGGCGTGGATCGACTCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGAcccg                                                         <  1:2890137/101‑1 (MQ=38)
                                              cgcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                                                        >  1:1421842/1‑101 (MQ=255)
                                              cgcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACgcgc                                                        <  1:2781863/101‑1 (MQ=255)
                                               gcgGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCt                                                       >  2:954915/1‑101 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     <  2:4686915/101‑1 (MQ=255)
                                                 gggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCACCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtca                                                     >  1:4106228/1‑101 (MQ=255)
                                                 aggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGGTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGCCGCGCtca                                                     >  1:22344/2‑101 (MQ=255)
                                                  ggggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcat                                                    <  2:4967708/101‑1 (MQ=255)
                                                   ggnCTTGGATCGAAACTNCAGAGTTCCGGTCTTGTTCTGTTCCGGTACCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   <  2:1000128/101‑1 (MQ=255)
                                                   gggCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCtcatc                                                   <  2:2381454/101‑1 (MQ=255)
                                                       gTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCa                                               <  1:1739455/101‑1 (MQ=255)
                                                        tGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAc                                              <  1:223607/101‑1 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCGCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             >  1:2573573/1‑101 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             <  2:3684529/101‑1 (MQ=255)
                                                         ggATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             >  1:4434546/1‑101 (MQ=255)
                                                         ggATCGAAACGACAGAGTTCCGGTCTTGTTCTGTGCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACg                                             <  2:4264287/101‑1 (MQ=255)
                                                           aTCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTc                                           <  2:729019/101‑1 (MQ=255)
                                                            tCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCa                                          >  1:4764779/1‑101 (MQ=255)
                                                              gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        <  1:587567/101‑1 (MQ=255)
                                                              gAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        <  2:1656463/101‑1 (MQ=255)
                                                              gAAACTACAGAGTTCCGGTCTTGTTCTGTGCAGGTAACAGGTCGCACCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAg                                        <  2:4739707/101‑1 (MQ=38)
                                                               aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       >  2:2726456/1‑101 (MQ=255)
                                                               aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       <  2:1449264/101‑1 (MQ=255)
                                                               aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       >  1:846906/1‑101 (MQ=255)
                                                               aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       <  1:836572/101‑1 (MQ=255)
                                                               aaaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGa                                       >  1:1169007/1‑101 (MQ=255)
                                                                aaCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAt                                      >  1:3788805/1‑101 (MQ=255)
                                                                 aCTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGAtt                                     <  1:651715/101‑1 (MQ=255)
                                                                  cTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTc                                    <  2:2522704/101‑1 (MQ=255)
                                                                    aCAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTAAAGATTCAg                                  >  2:2173414/1‑101 (MQ=255)
                                                                     cAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCAGGTCAAGATTCAGt                                 <  1:3371273/101‑1 (MQ=255)
                                                                      agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTc                                <  1:1099327/101‑1 (MQ=255)
                                                                      agagTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAATc                                >  2:1363793/1‑101 (MQ=255)
                                                                        agTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGc                              >  2:4365197/1‑101 (MQ=255)
                                                                          ttCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTc                            >  1:1580415/1‑101 (MQ=255)
                                                                           tcaggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcc                           >  2:4790784/4‑101 (MQ=255)
                                                                            ccGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccc                          >  2:14924/1‑101 (MQ=255)
                                                                             nggNCNTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                         <  1:1308944/100‑1 (MQ=255)
                                                                             cGGTCTTGTTCTGTTCCGGTAACAGGTCGCTTCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccc                         >  1:4506962/1‑101 (MQ=255)
                                                                              ggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCTTCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                        <  1:4336076/101‑1 (MQ=255)
                                                                              ggTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTccccc                        >  1:544108/1‑101 (MQ=255)
                                                                               gTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       <  1:3326575/101‑1 (MQ=255)
                                                                               gTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       <  1:4047984/101‑1 (MQ=255)
                                                                               gTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       >  2:4903161/1‑101 (MQ=255)
                                                                               gTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTcccccc                       >  1:2159749/1‑101 (MQ=255)
                                                                                tCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcg                      <  2:3943236/101‑1 (MQ=255)
                                                                                 cTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCcgc                     >  1:3656744/1‑101 (MQ=255)
                                                                                   tgttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgg                   >  1:4156354/1‑101 (MQ=255)
                                                                                    gttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg                  >  1:1340627/1‑101 (MQ=255)
                                                                                    gttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggg                  >  2:1896010/1‑101 (MQ=255)
                                                                                     ttctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCgggg                 >  2:2119148/1‑101 (MQ=255)
                                                                                      tctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCggggg                >  2:1167559/1‑101 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               >  2:3638488/1‑101 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               <  2:3356529/101‑1 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               >  2:2871769/1‑101 (MQ=255)
                                                                                       ctgttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGc               >  1:3139730/1‑101 (MQ=255)
                                                                                         gttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt             >  1:4073987/1‑101 (MQ=255)
                                                                                         gttcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGt             <  1:4000871/101‑1 (MQ=255)
                                                                                           tcCGGTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTgg           <  1:3455733/101‑1 (MQ=255)
                                                                                              ggTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATc        >  2:28864/1‑101 (MQ=255)
                                                                                               gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:943771/1‑101 (MQ=255)
                                                                                               gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  2:1231522/1‑101 (MQ=255)
                                                                                               gTAACAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCg       >  1:1625162/1‑101 (MQ=255)
                                                                                                  aCAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGaaa    <  2:3865971/101‑1 (MQ=255)
                                                                                                   cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   >  2:2242571/1‑101 (MQ=255)
                                                                                                   cAGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAc   <  2:3084576/101‑1 (MQ=255)
                                                                                                    aGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  <  1:2499663/101‑2 (MQ=255)
                                                                                                    aGGTCGCTCCCCCCGCGGGGGCGTGAATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAAct  >  2:453965/1‑100 (MQ=255)
                                                                                                    |                                                                                                    
CATCCGCGGATCGGCGTGACCCGCCCCGCCCATCGGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACTACAGAGTTCCGGTCTTGTTCTGTTCCGGTAACAGGTCGCTCCCCCCGCGGGCGCGTGGATCGAAACTTGTCTGACGCGCTCATCAGCACGTCAAGATTCAGTCGCTCCCCCCGCGGGGGCGTGGATCGAAACG  >  NC_014034/1397598‑1397798

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG