Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,044,270 Δ1 bp 89.8% intergenic (+143/+34) typA → / ← potG1 GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,044,2700A→.89.8% 63.9 ‑2.7 ‑1.0166intergenic (+143/+34)typA/potG1GTP‑binding protein TypA/BipA/polyamine ABC transporter ATP‑binding protein PotG
Reads supporting (aligned to +/- strand):  new base (119/30):  ref base (10/7):  total (129/37)
Fisher's exact test strand distribution p-value = 6.41e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.03e-01

Only 100 of 205 total aligned reads displayed.

CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCAC  >  NC_014034/2044170‑2044369
                                                                                                    |                                                                                                   
cGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCa                                                                                                     <  1:2652577/101‑1 (MQ=255)
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaa                                                                                                    <  2:134859/101‑1 (MQ=255)
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaa                                                                                                    <  1:3326880/101‑1 (MQ=255)
 gAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGAaa                                                                                                    >  2:4158864/1‑101 (MQ=255)
  aaTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaa                                                                                                   <  1:3309217/101‑1 (MQ=255)
   aTGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaa                                                                                                  >  1:1832352/1‑101 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                                <  2:1954357/101‑2 (MQ=255)
     gAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaag                                                                                                >  1:3876723/1‑100 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaagg                                                                                               <  1:340202/101‑3 (MQ=255)
      aaaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaaagg                                                                                               >  1:551843/1‑99 (MQ=255)
       aaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCaaaagatc                                                                                              >  2:4302352/1‑97 (MQ=255)
       aaTTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgg                                                                                              >  2:2471728/1‑101 (MQ=255)
          tCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGggggg                                                                                           >  2:3858153/1‑101 (MQ=255)
           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                          >  2:4911555/1‑100 (MQ=255)
           cACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAGgggggc                                                                                          <  2:2368918/101‑2 (MQ=255)
             ccTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGgcgc                                                                                       >  2:4244123/1‑101 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGcgcg                                                                                      >  1:1803026/1‑101 (MQ=255)
              cTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGcgcg                                                                                      <  1:4141502/101‑1 (MQ=255)
               tCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGa                                                                                     >  1:444007/1‑101 (MQ=255)
                ccccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGaa                                                                                    >  1:932006/1‑101 (MQ=255)
                 cccccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCAACTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAg                                                                                   <  2:826276/101‑1 (MQ=255)
                   cccGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcc                                                                                 <  2:1785979/101‑1 (MQ=255)
                     cGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcccc                                                                               >  1:596891/1‑101 (MQ=255)
                     cGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGcccc                                                                               <  1:366191/101‑1 (MQ=255)
                        gATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCt                                                                            >  1:970652/1‑101 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGTCGCGAAGCCCCCCtt                                                                           <  2:1746802/101‑1 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                           <  2:3134209/101‑1 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                           <  2:361022/101‑1 (MQ=255)
                         atatTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtt                                                                           >  1:1028637/1‑101 (MQ=255)
                          tatTTGAGGCAAGATAAAGCCTTGCGAGGTTTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttt                                                                          <  1:2173051/101‑1 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTTCGAGGTTTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttt                                                                         <  1:329594/101‑1 (MQ=255)
                           atTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttt                                                                         <  1:2285190/101‑1 (MQ=255)
                            tttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCttttt                                                                        >  2:1843642/1‑101 (MQ=255)
                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttc                                                                       >  1:5002533/1‑100 (MQ=255)
                             ttGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttc                                                                       >  2:679886/1‑100 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttcc                                                                      >  1:715668/1‑99 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttcc                                                                      >  1:2851268/1‑99 (MQ=255)
                              tGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttcc                                                                      >  1:4570160/1‑99 (MQ=255)
                                aGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCCtttttccgt                                                                    <  1:3997081/101‑5 (MQ=255)
                                 ggaaaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGCCCGCGGGGGAAATTTGCACAG.AAAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTc                                                                  <  2:701336/98‑1 (MQ=255)
                                 ggCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTc                                                                  >  1:4969620/1‑101 (MQ=255)
                                 ggCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTc                                                                  >  1:2604694/1‑101 (MQ=255)
                                  gCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCa                                                                 >  2:4693627/1‑101 (MQ=255)
                                   cAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAg                                                                >  2:4399284/1‑101 (MQ=255)
                                    aaGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGc                                                               >  2:2610792/1‑101 (MQ=255)
                                      gATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGa                                                             >  1:1099079/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                            >  2:1295277/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                            >  2:4481531/1‑101 (MQ=255)
                                       aTAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaa                                                            >  1:1080798/1‑101 (MQ=255)
                                        tAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGaaa                                                           >  2:3921525/1‑101 (MQ=255)
                                         agaGACTTGCGATGTTTTATACATCTTTCTAAAAAGACCGGGGGGGGAATTTACACAG.AAAAAGGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAAc                                                          <  2:4997289/99‑1 (MQ=255)
                                         aaaGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAAc                                                          >  1:3836343/1‑101 (MQ=255)
                                           aGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAg                                                        >  1:185952/1‑101 (MQ=255)
                                              ctgggggggTTTTCTTCATCTTTCTGAAAAGCCGGGGGGGGAAATTGGCACAG.AAAAAGGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCAc                                                     <  2:4016333/94‑1 (MQ=255)
                                              cTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCAc                                                     >  1:3127153/1‑101 (MQ=255)
                                                tGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCa                                                   >  2:2078353/1‑101 (MQ=255)
                                                 gCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAg                                                  >  1:1726787/1‑101 (MQ=255)
                                                  cGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgc                                                 >  2:2741010/1‑101 (MQ=255)
                                                   gAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcg                                                >  1:1257950/1‑101 (MQ=255)
                                                    aGGTGTTCTTCATCTTTCTGCTAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                               >  1:2877866/1‑101 (MQ=255)
                                                    aGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAgcgc                                               >  1:1554775/1‑101 (MQ=255)
                                                       tgtTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGcc                                            <  2:1631139/101‑1 (MQ=255)
                                                         ttcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCgg                                          >  1:4925820/1‑101 (MQ=255)
                                                          tcttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCggg                                         >  1:3038660/1‑101 (MQ=255)
                                                           cttcATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGc                                        >  1:4301218/1‑101 (MQ=255)
                                                                tCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                   >  2:1219471/1‑101 (MQ=255)
                                                                tCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCa                                   >  2:2914383/1‑101 (MQ=255)
                                                                 cTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                  >  1:1492912/1‑101 (MQ=255)
                                                                 cTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAg                                  >  1:4851194/1‑101 (MQ=255)
                                                                    tCTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGcc                               >  1:2953806/1‑101 (MQ=255)
                                                                    tCTGCAAAGACCGCGGGGGGAATTTGCACAG.AAAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGcc                               >  1:4474676/1‑101 (MQ=255)
                                                                     cTGCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGccc                              >  2:59565/1‑100 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                            >  1:2010703/1‑101 (MQ=255)
                                                                       gCAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAcc                            >  1:3916440/1‑101 (MQ=255)
                                                                       gCAAAGACCG.GGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAccc                           <  2:3173563/101‑1 (MQ=255)
                                                                        cAAAGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCAccc                           >  2:2044163/1‑101 (MQ=255)
                                                                         aaaGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcg                          >  2:3896885/1‑101 (MQ=255)
                                                                           aGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcgcg                        >  2:4604604/1‑101 (MQ=255)
                                                                           aGACCGCGGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCcccg                        >  2:3245399/1‑101 (MQ=255)
                                                                                gcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCATCGCGCCGGGCGACCAGGCCACCCGCGCCTgg                   >  1:1620886/1‑101 (MQ=255)
                                                                                gcgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTgg                   >  1:4710068/1‑101 (MQ=255)
                                                                                 cgGGGGGAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTggg                  >  1:2429022/1‑101 (MQ=255)
                                                                                   gggggAATTTTCCCAAAAAAAAGGGGGGCCGCG.ACCCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGt                 <  1:4519575/101‑1 (MQ=255)
                                                                                     gggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc              <  1:3884501/101‑1 (MQ=255)
                                                                                     gggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc              >  1:4321711/1‑101 (MQ=255)
                                                                                     gggAATTTGCACAG.AAAAAAGGGGGGCGCGACGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGcc              <  1:839638/101‑1 (MQ=255)
                                                                                      ggAATTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCg             >  1:1005986/1‑101 (MQ=255)
                                                                                        aaTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc           >  1:1854061/1‑101 (MQ=255)
                                                                                        aaTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc           >  1:3779586/1‑101 (MQ=255)
                                                                                        aaTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc           >  2:4332023/1‑101 (MQ=255)
                                                                                        aaTTGGCACAG.AAAAAGGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGAc           <  2:2699792/101‑1 (MQ=255)
                                                                                         aTTTGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACa          >  1:2702859/1‑101 (MQ=255)
                                                                                          tttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACAt         >  2:1518383/1‑101 (MQ=255)
                                                                                           ttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc        >  1:2801788/1‑101 (MQ=255)
                                                                                           ttGCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc        >  2:3568447/1‑101 (MQ=255)
                                                                                           tgccacaGC.AAAAGGGGGGGCGC.AAACCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATc        <  2:2118251/98‑1 (MQ=255)
                                                                                             gCACAGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCa      <  1:4028920/101‑1 (MQ=255)
                                                                                              cacaGC.AAAAAGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAg     <  2:144750/101‑1 (MQ=255)
                                                                                                acagaaaaaaGGGGGGCGCGAA.CCCCCCCTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGc    <  1:4809021/97‑1 (MQ=255)
                                                                                                  agaaaaagGGGGGGCGCGAAGCCCCCC.TTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCAc  <  2:2978203/99‑1 (MQ=255)
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CGAATGAAATTCACCTCCCCCCGGGATATTTGAGGCAAGATAAAGACTTGCGAGGTGTTCTTCATCTTTCTGCAAAGACCGCGGGGGGAATTTGCACAGCAAAAAAGGGGGGCGCGAAGCCCCCCTTTTTTCCGTCAGCGAAACAGCACCAGCGCGCCGGGCGACCAGGCCACCCGCGTCTGGGTGCCGACATCCAGCAC  >  NC_014034/2044170‑2044369

Base quality scores:  ATCG  < 3 ≤  ATCG  < 21 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG