Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,180,217 Δ1 bp 100% coding (906/1314 nt) RCAP_rcc02022 → polyols ABC transporter periplasmic polyols‑binding protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,180,2170C→.100.0% 862.2 197coding (906/1314 nt)RCAP_rcc02022polyols ABC transporter periplasmic polyols‑binding protein
Reads supporting (aligned to +/- strand):  new base (82/114):  ref base (0/0):  total (82/115)

Only 100 of 217 total aligned reads displayed.

CCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAA  >  NC_014034/2180120‑2180315
                                                                                                    |                                                                                                  
ccAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAc                                                                                                    >  1:2100317/1‑101 (MQ=255)
 cAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcc                                                                                                   <  1:3046305/101‑1 (MQ=255)
  aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                                                  <  1:1035836/101‑2 (MQ=255)
  aaCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcct                                                                                                  <  2:305977/101‑2 (MQ=255)
   aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                                                 <  1:630211/101‑3 (MQ=255)
   aCCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctc                                                                                                 <  2:1703240/101‑3 (MQ=255)
     cccAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAAcctcgc                                                                                               >  1:116145/1‑97 (MQ=255)
         aGGACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCatt                                                                                          <  1:1418872/101‑3 (MQ=255)
          ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCTTTGACGCCGAACAAGGCCGGTGTTGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                         <  1:4318214/101‑4 (MQ=255)
          ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                         <  1:1131603/101‑4 (MQ=255)
          ggACTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCCattc                                                                                         >  1:1482941/1‑98 (MQ=255)
            acgcGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcg                                                                                      <  2:2382668/98‑1 (MQ=255)
             cTCGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcgg                                                                                     >  1:3295529/1‑101 (MQ=255)
               cGGCCGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCcggcg                                                                                   >  1:3925950/1‑101 (MQ=255)
                  ccGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGc                                                                                <  1:1892914/101‑1 (MQ=255)
                   cGTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGACTGGAA.CCTCGCC.ATTCCGGCGGGcc                                                                               >  2:2910233/1‑100 (MQ=255)
                    gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                              >  1:1984588/1‑101 (MQ=255)
                    gTGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCAc                                                                              <  1:885222/101‑1 (MQ=255)
                     tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                             <  2:4804758/101‑1 (MQ=255)
                     tGGCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACg                                                                             <  1:2753416/101‑1 (MQ=255)
                       gCCGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCa                                                                           <  1:535428/101‑1 (MQ=255)
                         cGATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaa                                                                         <  2:726826/101‑1 (MQ=255)
                          gATCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaa                                                                        >  1:3197943/1‑101 (MQ=255)
                           aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaag                                                                       >  2:4473588/1‑100 (MQ=255)
                           aTCATGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGACTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaagg                                                                       >  1:1193178/1‑99 (MQ=255)
                              aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                    >  2:2753394/1‑97 (MQ=255)
                              aTGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGCaaaaggcc                                                                    >  1:412862/1‑97 (MQ=255)
                               tGTCGGCTTCGCGCTGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCg                                                                  <  2:928749/101‑1 (MQ=255)
                                      ttcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAggcggc                                                           <  2:4384277/96‑1 (MQ=255)
                                       tcgcgctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGgcggcg                                                          >  2:405501/5‑101 (MQ=255)
                                           gctGGCGCCGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGa                                                      >  2:2251504/1‑101 (MQ=255)
                                            ctggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                     <  1:1042953/95‑1 (MQ=255)
                                            ctcgggccGAACAAGGCCGGGGTCGCCAAGCATGGGAACTGGCTCTGGGCCTGTAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaa                                                     <  2:4431954/95‑1 (MQ=255)
                                             tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                    >  2:3704316/6‑101 (MQ=255)
                                             tggcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGaaa                                                    >  1:4540466/6‑101 (MQ=255)
                                               gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                  <  2:436819/98‑1 (MQ=255)
                                               gcgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAAtt                                                  <  2:2193805/98‑1 (MQ=255)
                                                cgccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtc                                                 <  1:4074258/99‑1 (MQ=255)
                                                  ccGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgt                                               >  1:4180469/1‑101 (MQ=255)
                                                   cGAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtc                                              >  1:1235454/1‑101 (MQ=255)
                                                    gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                             <  2:3915830/101‑1 (MQ=255)
                                                    gAACAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATtcgtcg                                             >  2:1161463/1‑101 (MQ=255)
                                                     aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            <  1:3552808/101‑1 (MQ=255)
                                                     aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            >  1:1142805/1‑101 (MQ=255)
                                                     aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            <  1:3879411/101‑1 (MQ=255)
                                                     aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            >  2:2338451/1‑101 (MQ=255)
                                                     aaCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            >  2:1640578/1‑101 (MQ=255)
                                                     aaCAAGGCCGGTGTCGCCAAGCATGGCAACCGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGa                                            >  1:378804/1‑101 (MQ=255)
                                                      aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGaa                                           >  1:1695059/1‑101 (MQ=255)
                                                      aCAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGCCGaa                                           >  2:3393675/1‑101 (MQ=255)
                                                       cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGTCGTGGCGGCGCTCAAATTCGTCGAAt                                          >  2:2794578/1‑101 (MQ=255)
                                                       cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                          >  1:1777984/1‑101 (MQ=255)
                                                       cAAGGCCGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAAt                                          <  1:2667471/101‑1 (MQ=255)
                                                         aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                        <  1:2910446/97‑1 (MQ=255)
                                                         aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                        <  1:3387200/97‑1 (MQ=255)
                                                         aggccGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATgg                                        <  2:1156240/97‑1 (MQ=255)
                                                           gccGGTGTCGCCAAGCATNGCAACTGGCTCNGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGc                                      <  1:1918811/99‑1 (MQ=255)
                                                             cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                    <  2:4569477/101‑1 (MQ=255)
                                                             cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                    <  2:4698871/101‑1 (MQ=255)
                                                             cGGTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGa                                    >  2:2120952/1‑101 (MQ=255)
                                                              ggTGTCGCCAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGAc                                   >  1:2348084/1‑101 (MQ=255)
                                                                  tcgccAAGTATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCTGCGCTGAAATTCGTCGAATGGGCGACCTcc                               <  1:3626809/97‑1 (MQ=255)
                                                                  tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                               <  2:4633154/97‑1 (MQ=255)
                                                                  tcgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTcc                               <  2:533213/97‑1 (MQ=255)
                                                                   cgccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGACGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCa                              >  2:3299138/4‑101 (MQ=255)
                                                                    gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                             <  1:795205/99‑1 (MQ=255)
                                                                    gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                             >  1:964448/3‑101 (MQ=255)
                                                                    gccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCaa                             <  1:942706/99‑1 (MQ=255)
                                                                    gccAAGCATGGCAACTGCCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTAAAATTCGTCGAATGGGCGACCTCCaa                             <  2:4541642/99‑1 (MQ=255)
                                                                     ccAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAg                            >  2:1092416/2‑101 (MQ=255)
                                                                      cAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAgg                           <  2:1922759/101‑1 (MQ=255)
                                                                      cAAGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGCCCGCGGCGGCGCTGAAATTCGTCGAACGGGCGCCCTCCAAgg                           >  1:159220/1‑101 (MQ=255)
                                                                       aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                          <  1:2264813/101‑1 (MQ=255)
                                                                       aaGCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGa                          <  2:1482811/101‑1 (MQ=255)
                                                                         gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                        <  1:2155953/101‑1 (MQ=255)
                                                                         gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                        <  2:1272684/101‑1 (MQ=255)
                                                                         gCATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACt                        >  1:3712502/1‑101 (MQ=255)
                                                                          cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGGGGCGGTGAAATTCGTCGAATGGGCGACCTCCAAGGACtc                       >  2:2039429/1‑100 (MQ=255)
                                                                          cATGGCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTa                       <  2:4215000/101‑1 (MQ=255)
                                                                              gCAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACAcc                   <  1:4059583/101‑1 (MQ=255)
                                                                               cAACTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCg                  <  2:3552184/101‑1 (MQ=255)
                                                                                aaCTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGc                 <  1:706378/101‑1 (MQ=255)
                                                                                  cTGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGccc               <  2:1212563/101‑1 (MQ=255)
                                                                                   tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt              >  2:798311/1‑101 (MQ=255)
                                                                                   tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCt              <  1:2166779/101‑1 (MQ=255)
                                                                                   tGGCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTAAACCGCCCt              >  2:4971320/1‑101 (MQ=255)
                                                                                    ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCtt             <  1:750000/101‑1 (MQ=255)
                                                                                    ggCTCTGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCAACCTCCAAGGACTACACCGCCCtt             >  2:3086346/1‑101 (MQ=255)
                                                                                      ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCttat           <  2:4122632/101‑3 (MQ=255)
                                                                                      ctctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGt           >  2:3271371/1‑101 (MQ=255)
                                                                                       tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc          <  1:4781046/101‑1 (MQ=255)
                                                                                       tctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTc          <  2:700825/101‑1 (MQ=255)
                                                                                        ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGGCGAGGCGGGGCTGAAATTCGTCGAATGGGCGAACTCCAAGGGCTACACCGCCCTTGTCg         >  2:2654901/1‑101 (MQ=255)
                                                                                        ctGGGCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCg         >  1:4227743/1‑101 (MQ=255)
                                                                                           ggCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCa      <  2:2100169/101‑1 (MQ=255)
                                                                                            gCCTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAg     <  2:195887/101‑1 (MQ=255)
                                                                                             ccTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGc    <  2:3817582/101‑1 (MQ=255)
                                                                                              cTGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCa   >  2:3458434/1‑101 (MQ=255)
                                                                                               tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCaa  >  2:1469491/1‑101 (MQ=255)
                                                                                               tGGAA.CCTCGCC.ATTCCGGCGGGCACGC.AAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCCCCCTTGTCGCCAGCaa  >  1:2221598/1‑101 (MQ=255)
                                                                                                    |                                                                                                  
CCAACCCCAAGGACTCGGCCGTGGCCGATCATGTCGGCTTCG.GCTGGC.CCGAACAAGG.CGGTGTCCGCAAGCATGGCAACTGGCTCTGGGCCTGGAACCCTCGCCAATTCCGGCGGGCACGCAAAAAGGCCGAGGCGGCGCTGAAATTCGTCGAATGGGCGACCTCCAAGGACTACACCGCCCTTGTCGCCAGCAA  >  NC_014034/2180120‑2180315

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG