Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	NC_014034	2,185,997	Δ2 bp	100%	intergenic (+270/‑234)	mtlK → / → RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein

Read alignment evidence...
	seq id	position		change	freq	score	reads	annotation	genes	product
*	NC_014034	2,185,997	0	G→.	100.0%	18.8	11	intergenic (+270/‑235)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (0/11):  ref base (0/0):  total (0/11)
*	NC_014034	2,185,998	0	C→.	100.0%	18.8	11	intergenic (+271/‑234)	mtlK/RCAP_rcc02028	mannitol 2‑dehydrogenase/FeoA family protein
Reads supporting (aligned to +/- strand):  new base (0/11):  ref base (0/0):  total (0/11)

ACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC...GC.CGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCA  >  NC_014034/2185920‑2186071                                                                                       ||                                                                          aCGCCGACCCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACAcccc                                                      <  2:4712730/101‑1 (MQ=2)      gAACCGCCCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGGCGCCGCCGGG.G.AAGGCCGAGGCGGAAAAcccccccgc                                                 >  1:638168/1‑96 (MQ=2)         ccccccccTTCCACCTTGGCCAAAAGACCCCGGGGGCCGGGGGCCGTA..GC.CCC...TCGCGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCac                                              <  2:4195431/97‑1 (MQ=2)                 tttCACCTGGGCTCAAATACCCCGGGGGGCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACcgaccgac                                      <  1:1354669/101‑1 (MQ=9)                     cccTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                  <  2:2483931/100‑1 (MQ=9)                     cccTTGGCTCAAATACCCGGGGGGCCGGGGGGCGGACCC..CCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGc                                  <  2:4581361/100‑1 (MQ=9)                      ccTTGGCTCAAATACCCCGGGGGTCCGGGGGGGGACCC..CCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCg                                 <  2:2305330/101‑1 (MQ=9)                        ttGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGAc                               <  1:2471680/101‑1 (MQ=14)                                   atCCCGGGGGTCCGGGGGCGGA..GCCCCC...GG.CGGGGCGCCCCCGG..CCAAGCCCCAGGCGAAAAACCCCAGCGCCCCCCCCCCACCTGCGACGGCCCGGcgcg                    >  2:4058630/1‑101 (MQ=2)                                     cccGGGGGGCCGGGGGCGGA..G..CCCCCGGG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCgcgc                   <  1:737388/101‑1 (MQ=12)                                        gggggTCCGGGGGCGGA..GCCCCC...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCaaaa               <  1:1619847/101‑1 (MQ=18)                                                     ggcggacccccc...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcagg    <  2:631719/92‑1 (MQ=21)                                                       cggagccccc...GG.CGGGTCGCCTCGGGCA..AAGCCCGAGGCGAAACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCcaggca  <  1:2714778/94‑1 (MQ=21)                                                                                       ||                                                                          ACGCCGAACCG.CCCCTTTCACCTTGGCTCAAATATCCCGGGGGTCCGGGGGCGGA..G..CCC...GC.CGGGTCTCCTCCGGGAGCAAGGCCGAGGCGAGACACCCCAGCGCCACCGACCGACTTGCGACGGCCTGTCGCGCAAAAGCCCGGCCAGGCA  >  NC_014034/2185920‑2186071 Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG