Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,566,597 Δ1 bp 2.3% intergenic (+69/+125) purD → / ← sdaA phosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,566,5970A→. 2.3% 6.7 0.0 0.0217intergenic (+69/+125)purD/sdaAphosphoribosylamine‑‑glycine ligase/L‑serine ammonia‑lyase
Reads supporting (aligned to +/- strand):  new base (2/3):  ref base (91/121):  total (93/124)
Fisher's exact test strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.00e+00

Only 100 of 249 total aligned reads displayed.

GCCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGC  >  NC_014034/2566500‑2566697
                                                                                                     |                                                                                                      
gccgcGACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATa                                                                                                        <  2:2373027/101‑1 (MQ=21)
 ccgcGACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                                                                       <  1:655313/101‑1 (MQ=21)
 ccgcGACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaa                                                                                                       <  1:747985/101‑1 (MQ=21)
  cgcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATaaa                                                                                                      <  1:4025861/101‑1 (MQ=21)
   gcgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaag                                                                                                     <  2:641627/101‑1 (MQ=21)
    cgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                                                                                    <  2:2991831/101‑1 (MQ=21)
    cgACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaaga                                                                                                    <  2:2589035/101‑1 (MQ=21)
     gACATCGGCTGGCGCTCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGGCCAATCACCCCCCCGGGTAATTCGGGCCAGATAaaaaa                                                                                                   >  1:2354133/1‑101 (MQ=12)
     gACATCGGCTGGCGCGCGCTCTGGGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAaagaa                                                                                                   <  2:1840456/101‑1 (MQ=21)
     gACATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCAACCCCCGGGATATTTCGGGCAAGATAaagaa                                                                                                   <  1:1375187/101‑1 (MQ=21)
       cATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                                                                                 <  2:3278843/101‑1 (MQ=21)
       cATCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAgg                                                                                                 <  1:48548/101‑1 (MQ=21)
         tCGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTc                                                                                               <  2:2440729/101‑1 (MQ=21)
          cGGCTGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCt                                                                                              >  1:598450/1‑101 (MQ=21)
              tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                          <  2:1768660/101‑1 (MQ=21)
              tGGCGCGCGCTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTc                                                                                          <  2:3994153/101‑1 (MQ=21)
               ggCGCGCGCTCTGAAACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTcc                                                                                         <  1:3651329/101‑1 (MQ=21)
                 cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                       <  1:2093234/101‑1 (MQ=21)
                 cgcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCtt                                                                                       <  1:226240/101‑1 (MQ=21)
                  gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                      <  1:3186209/101‑1 (MQ=21)
                  gcgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCttt                                                                                      <  1:4426888/101‑1 (MQ=21)
                   cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                     <  2:406436/101‑1 (MQ=21)
                   cgcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTc                                                                                     <  2:2870611/101‑1 (MQ=21)
                    gcgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCa                                                                                    <  1:2952744/101‑1 (MQ=21)
                     cgcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAt                                                                                   <  1:3208980/101‑1 (MQ=21)
                      gcTCTGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATc                                                                                  <  1:2565977/101‑1 (MQ=21)
                       ctctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.t                                                                                >  2:2387314/1‑101 (MQ=21)
                       ctctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.t                                                                                >  2:4678419/1‑101 (MQ=21)
                        tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.tt                                                                               <  1:88760/101‑1 (MQ=21)
                        tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.tt                                                                               >  2:4397614/1‑101 (MQ=21)
                        tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.tt                                                                               >  2:612440/1‑101 (MQ=21)
                        tctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTCTCCTTTCATC.tt                                                                               <  1:2728105/101‑1 (MQ=21)
                         ctGAGACGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTATCCTTTCATC.TTg                                                                              <  2:1974418/101‑1 (MQ=21)
                            agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGccc                                                                           <  1:1748694/101‑1 (MQ=21)
                            agaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGccc                                                                           <  1:191171/101‑1 (MQ=21)
                             gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCg                                                                          <  1:514012/101‑1 (MQ=21)
                             gaCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCg                                                                          <  2:3407080/101‑1 (MQ=21)
                              aCGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.a                                                                        <  2:539174/101‑1 (MQ=21)
                               cGAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.aa                                                                       <  1:1617993/101‑1 (MQ=21)
                                gAAACGGGGGGCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.aaa                                                                      >  2:229319/1‑101 (MQ=21)
                                 aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAat                                                                     <  1:3944356/96‑1 (MQ=21)
                                 aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAat                                                                     >  1:3827214/6‑101 (MQ=21)
                                 aaacggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAat                                                                     <  2:107585/96‑1 (MQ=21)
                                   acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCTCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAatat                                                                   <  2:3198269/98‑1 (MQ=25)
                                   acggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAatat                                                                   <  1:2129400/98‑1 (MQ=25)
                                    cggggggCGCTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTACTTTCATC.TTGCCCG.AAATATc                                                                  >  2:1100723/3‑101 (MQ=21)
                                      gggggCGCTGCCCCCTCTTGGC..CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATC.TTGCCGC.AAATATCCCgggggg                                                          <  2:4231871/101‑2 (MQ=0)
                                      gggggCGCTG.CCCC.CTCTTGGCCTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATC.TTGCCGC.AAATATCCCgggggg                                                          >  2:1480212/1‑100 (MQ=0)
                                       ggggCCCTGCCCCC.TTTTGGC.CTTTGGCCAATTCA.CCCCTGGGATATTTCGGGCAAGAT...GAA.ATCATGCCCCTTCATC.TTGCCGC.AAATATCCCgggggga                                                         <  2:359618/101‑3 (MQ=2)
                                          gcgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCgggg                                                            >  1:1121439/1‑101 (MQ=37)
                                           cgcTGCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCggggg                                                           <  2:4869814/101‑1 (MQ=37)
                                               gCCCCC.CTCTTGGCCTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGGCCG.AAATATCCCGGGGGAGcc                                                       >  2:4973558/1‑101 (MQ=25)
                                                    ccctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCt                                                   <  1:2645069/91‑1 (MQ=255)
                                                     cctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCtt                                                  <  2:3833724/92‑1 (MQ=255)
                                                     cctcctggccTTCGCCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCtt                                                  <  1:3930720/92‑1 (MQ=37)
                                                      ctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTg                                                 <  1:3269055/93‑1 (MQ=255)
                                                      ctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTg                                                 <  2:2205523/93‑1 (MQ=255)
                                                      ctcttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTg                                                 <  1:1577742/93‑1 (MQ=255)
                                                        cttggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcg                                               >  2:2497216/7‑101 (MQ=255)
                                                          tggccTTCGGCCAATTCCCCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcggg                                             <  2:4504973/97‑1 (MQ=255)
                                                          tggccTTCGGCCAATTCACCCCCCGGGATAATTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AGATATCCCGGGGGAGCCCGCTTgcggg                                             >  2:1612481/5‑101 (MQ=255)
                                                           ggccTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTgcgggc                                            >  1:2694411/4‑101 (MQ=255)
                                                             ccTTCGGCCAATTCACCCCCCGGGATAATTTGGGCAAGATAAAAAAAGGGTTTTTCTTTTATCTTTTCCCC.AAAATCCCCGGGGGGGCCCCCTGgcggggg                                           >  1:4235308/2‑99 (MQ=12)
                                                                 cGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGc                                      >  1:2183399/1‑101 (MQ=255)
                                                                  ggCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                     >  2:1382929/1‑101 (MQ=255)
                                                                   ggcAAATCCCCCCCCGGGGTTTTTTCGGGAAAAAAAAAAAGGGTTTTTTCTTTCCTT.TTTCCCGAAAATTCCCCGGGGGAGCCCGCTTGCGGGCGGGGGCa                                     <  1:1020567/99‑1 (MQ=255)
                                                                       aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                >  1:1842656/1‑101 (MQ=255)
                                                                       aTTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGGCTTTTTCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcc                                >  2:2525011/1‑101 (MQ=255)
                                                                         tccccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                              <  1:967887/98‑1 (MQ=255)
                                                                         tCACCCCCCGGCATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCAGC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGcccc                              <  2:3964271/101‑1 (MQ=255)
                                                                          cACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGccccc                             >  1:3361587/1‑101 (MQ=255)
                                                                           gccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCg                            <  1:554365/100‑1 (MQ=255)
                                                                            ccccccGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGa                           <  2:1654836/101‑1 (MQ=255)
                                                                                 cGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGCAgcgc                      >  2:753876/1‑101 (MQ=255)
                                                                                  gggATATTTCGGTCAATATAAAGAAGGTCTTTTCCTTTCTTC.TTGCCCA.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCATCGCCCCCGAAGCGcc                     <  1:1987380/101‑1 (MQ=255)
                                                                                  gggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGcc                     >  1:3596420/1‑101 (MQ=255)
                                                                                   ggATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTTCGGGCGGGGGCAGGGCCCCCCAAGGGccc                    >  2:3462992/1‑101 (MQ=38)
                                                                                     atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                  >  1:2907800/1‑101 (MQ=255)
                                                                                     atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGa                  >  2:3544099/1‑101 (MQ=255)
                                                                                     atatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCCCCCGa                  >  2:1511156/1‑101 (MQ=255)
                                                                                      tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                 >  2:3366566/1‑101 (MQ=255)
                                                                                      tatTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAc                 >  2:2717608/1‑101 (MQ=255)
                                                                                       atTTCGTGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                <  1:812310/101‑1 (MQ=255)
                                                                                       atTTCGGGCAAGATAGAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcg                >  1:4256987/1‑101 (MQ=255)
                                                                                        tttCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGAcgc               <  2:4650965/101‑1 (MQ=255)
                                                                                            gggCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg           >  1:466027/1‑101 (MQ=255)
                                                                                            gggCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcgg           >  2:3133509/1‑101 (MQ=255)
                                                                                             ggCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggc          <  1:3030695/101‑1 (MQ=255)
                                                                                              gCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCCTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCgcggcg         >  1:4711090/1‑101 (MQ=255)
                                                                                                aaGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCggg       >  2:88232/1‑101 (MQ=255)
                                                                                                 aGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGc      >  1:3104665/1‑101 (MQ=255)
                                                                                                  gATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt     >  2:2084809/1‑101 (MQ=255)
                                                                                                  gATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCt     >  2:2167219/1‑101 (MQ=255)
                                                                                                   aTAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt    >  1:2990843/1‑101 (MQ=255)
                                                                                                   aTAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt    >  2:484452/1‑101 (MQ=255)
                                                                                                   aTAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt    >  2:3760075/1‑101 (MQ=255)
                                                                                                   aTAAAGAAGGTCTTTTCCTTTCATC.TTACACG.AACTGTCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCtt    <  2:4754042/101‑1 (MQ=255)
                                                                                                    tAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTg   >  1:3308627/1‑101 (MQ=255)
                                                                                                     aaaGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc  >  1:2442162/1‑101 (MQ=255)
                                                                                                     aaaGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTgc  >  2:4333584/1‑101 (MQ=255)
                                                                                                     |                                                                                                      
GCCGCGACATCGGCTGGCGCGCGCTCTGAGACGAAAC.GGGGGCGCTGCCCCC.CTCCTG..CTTCGGCCAATTCACCCCCCGGGATATTTCGGGCAAGATAAAGAAGGTCTTTTCCTTTCATC.TTGCCCG.AAATATCCCGGGGGAGCCCGCTTGCGGGCGGGGGCAGCGCCCCCGAAGCGCCCGACGCGCGGCGGGCTTGC  >  NC_014034/2566500‑2566697

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG