Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 2,640,201 Δ1 bp 100% intergenic (‑177/+99) thrS ← / ← RCAP_rcc02462 threonyl‑tRNA synthetase/hypothetical protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140342,640,2010A→.100.0% 118.7 206intergenic (‑177/+99)thrS/RCAP_rcc02462threonyl‑tRNA synthetase/hypothetical protein
Reads supporting (aligned to +/- strand):  new base (77/100):  ref base (15/14):  total (92/114)
Fisher's exact test strand distribution p-value = 4.27e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 2.41e-03

Only 100 of 224 total aligned reads displayed.

AGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGG.CTTC.GGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297
                                                                                                       |                                                                                                
aGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGa                                                                                                  <  1:1754155/101‑1 (MQ=25)
 ggCGTGTCAACCGTGGCGC.TTTGGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                 <  2:3870815/101‑1 (MQ=25)
 gccGTGCCAACCGT.GCGCTTTTTGCG.AGGCAAAAGGGGGGGCCCTCCCCCCCCCTGGC.CTTCGGGCAAATTCCCCCCCCGAGGTATTTGGAGCAAGATGaa                                                                                                 <  2:1571107/99‑1 (MQ=255)
   cGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaag                                                                                               <  1:434024/101‑2 (MQ=25)
    gtgtCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATGaaaga                                                                                              <  2:202336/101‑3 (MQ=25)
       tCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCGCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                                          >  2:1060519/1‑101 (MQ=21)
       tCAACCGTGGCGC.TTTGGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCa                                                                                          <  2:810723/101‑1 (MQ=21)
        cAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCAt                                                                                         <  2:4332313/101‑1 (MQ=25)
         aaCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATg                                                                                        <  1:2753977/101‑1 (MQ=25)
          aCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                       <  2:2917796/101‑1 (MQ=25)
          aCCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATgg                                                                                       <  1:3436251/101‑1 (MQ=25)
              tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:1368349/101‑1 (MQ=25)
              tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:1426972/101‑1 (MQ=25)
              tGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTc                                                                                   <  1:2159917/101‑1 (MQ=25)
               ggCGC.TTTTGCGCCGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCAAGGAATTTGGAGCAAGATG.AAAGACCATGGGTTCa                                                                                  <  2:879748/101‑1 (MQ=21)
                gcgc.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCAt                                                                                 <  2:1596138/101‑1 (MQ=25)
                  gc.TTTTGCGCAGGCAAAA.GGGGGGGGCTGCCCCCCTCCTGGCCCTC.GGCCGCTTCCCCCCCCCGGGGATTTGGAGGCAGATG.AAAGACCATTGGGTCATCt                                                                               >  1:4922378/1‑101 (MQ=255)
                  gc.TTTGGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCt                                                                               <  2:1492289/101‑1 (MQ=21)
                     ttttGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTg                                                                             <  2:4296686/101‑1 (MQ=25)
                       ttGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGcc                                                                           <  1:4656310/101‑1 (MQ=25)
                        tGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                          <  1:2189074/101‑1 (MQ=25)
                        tGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCt                                                                          <  1:4611862/101‑1 (MQ=25)
                         gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:691865/101‑1 (MQ=25)
                         gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:4826725/101‑1 (MQ=25)
                         gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:2550142/101‑1 (MQ=25)
                         gcgcAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTc                                                                         <  2:4418752/101‑1 (MQ=25)
                          ggcAGGCAAAA.CGGTGGCGCTGCCGCCGCCTTGGCCTTC.CGGGAATTGACCCCCCGAGGTGTTTGGGGGAAGATG.AAAGGCCATGGGTTCATCTTGCCTCa                                                                        <  2:3737374/100‑1 (MQ=255)
                           gcAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaa                                                                       <  1:4774475/101‑1 (MQ=25)
                            gccccgaaa.AGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                     <  1:3887285/95‑1 (MQ=14)
                            gccccgaaa.AGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAat                                                                     <  2:2131694/95‑1 (MQ=14)
                            cAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCaaa                                                                      <  1:2353777/101‑1 (MQ=21)
                             aGGCAAAA.GGGGGGCGCTGCCACCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAat                                                                     <  1:1852141/101‑1 (MQ=25)
                              ggCAAAA.GGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCACCTTGCCTCAAata                                                                    <  1:3238144/101‑1 (MQ=21)
                                 gaaa.AGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATccc                                                                >  1:3780954/2‑101 (MQ=17)
                                  aaa.AGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               <  2:2360025/101‑1 (MQ=17)
                                  aaa.AGGGGGCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCg                                                               >  1:2578103/1‑101 (MQ=17)
                                    aaagggggCGCTGCCCCCCTCTTGGCCTTT.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgg                                                              >  1:4509162/4‑101 (MQ=18)
                                       gggggCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCCGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCCAAAATCCCcgggg                                                           >  1:4414993/1‑101 (MQ=12)
                                        ggggCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCCCCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                          <  1:106036/101‑2 (MQ=18)
                                        ggggCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggt                                                          <  1:4633495/101‑2 (MQ=18)
                                        ggggCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggg                                                          >  2:2845192/1‑101 (MQ=18)
                                         gggCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCggtggg                                                          <  2:3580942/101‑1 (MQ=21)
                                          ggCGCTGCCCCCCTCTTGGCCTTC.GGCCAATTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGa                                                         <  1:3928763/101‑1 (MQ=25)
                                             gcTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                     >  2:1413667/1‑95 (MQ=21)
                                             gcTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGGGATATTTGCGGCAAGGTGAAAGGA..TGGGGTTTATCTTGCCCCAAATATCCCgggggtgaggc                                                     >  2:3701700/1‑95 (MQ=21)
                                             gcTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTc                                                      <  2:1463124/101‑1 (MQ=25)
                                             gcTGCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCCGGATATTTGCGGCAAGGTGAAAGGA.TGGGGGTT.ATCTTGCCCCAAATATCCCgggggggaggg                                                     >  2:3599364/1‑96 (MQ=18)
                                                gCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   <  2:4038199/101‑1 (MQ=37)
                                                gCCCCCCTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTg                                                   <  1:1181267/101‑1 (MQ=37)
                                                   ccccTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGaaa                                                <  2:4965158/101‑1 (MQ=37)
                                                     ccTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              >  2:3890968/1‑101 (MQ=37)
                                                     ccTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              <  2:4643736/101‑1 (MQ=37)
                                                     ccTCTTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAgg                                              >  2:4735993/1‑101 (MQ=37)
                                                      ctctTGGCCTTC.GGCCATTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  2:1866864/1‑101 (MQ=25)
                                                      ctctTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGa                                             >  1:2784059/1‑101 (MQ=37)
                                                        ctTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                           >  1:2970171/1‑101 (MQ=37)
                                                        ctTGGCCTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACg                                           <  2:1247148/101‑1 (MQ=37)
                                                          tggccTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  1:1493249/5‑101 (MQ=37)
                                                          tggccTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACggg                                         >  2:1516698/5‑101 (MQ=37)
                                                           ggccTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACgggg                                        >  1:2542152/4‑101 (MQ=37)
                                                              cTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     >  2:3119214/1‑101 (MQ=37)
                                                              cTTC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGc                                     <  2:1544919/101‑1 (MQ=255)
                                                               ttC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCa                                    >  1:4739035/1‑101 (MQ=37)
                                                                tC.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCag                                   <  1:2622072/101‑1 (MQ=255)
                                                                 c.GGCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCaga                                  <  1:3141005/101‑1 (MQ=255)
                                                                   ggcgtactcacccgccGAAGTATTTGGAGGAAGATG.AAAGGCCCTGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 <  1:50796/91‑1 (MQ=18)
                                                                   cgCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCagag                                 <  2:4922051/100‑1 (MQ=37)
                                                                    gCCAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGc                                >  2:1312392/1‑101 (MQ=255)
                                                                      cAATTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccc                              >  1:4019638/1‑101 (MQ=37)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:551824/1‑101 (MQ=37)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             <  2:2210/101‑1 (MQ=255)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  1:2937973/1‑101 (MQ=255)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  2:3002397/1‑101 (MQ=255)
                                                                       aaTTCACCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGcccc                             >  1:3586543/1‑101 (MQ=37)
                                                                        aTTCCCCCCCCGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGccccc                            <  2:4227003/101‑1 (MQ=255)
                                                                          tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  1:593444/4‑101 (MQ=255)
                                                                          tcaccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCt                          >  2:4149093/4‑101 (MQ=25)
                                                                           caccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTc                         >  2:1731516/3‑101 (MQ=255)
                                                                             ccccccGATGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       >  1:2272521/1‑101 (MQ=25)
                                                                             ccccccGAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  2:3200410/101‑1 (MQ=255)
                                                                             ccccccGAGGTATTTGGAGCAAGATG.AAAGACCAGGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTccc                       <  1:3561985/101‑1 (MQ=255)
                                                                                   gAGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCt                 <  2:4602737/101‑1 (MQ=255)
                                                                                    aGGTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCtt                <  2:353187/101‑1 (MQ=255)
                                                                                      gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  2:3679798/1‑101 (MQ=255)
                                                                                      gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  1:969299/1‑101 (MQ=255)
                                                                                      gTATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtc              >  1:3055984/1‑101 (MQ=255)
                                                                                       tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  1:4479855/1‑101 (MQ=255)
                                                                                       tATTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtct             >  2:4649427/1‑101 (MQ=255)
                                                                                        aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            <  1:711243/101‑1 (MQ=255)
                                                                                        aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            <  2:267479/101‑1 (MQ=255)
                                                                                        aTTTGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTtctc            >  1:4248801/1‑101 (MQ=255)
                                                                                         tttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCa           <  1:2907637/101‑1 (MQ=255)
                                                                                          ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          >  1:1101441/1‑101 (MQ=255)
                                                                                          ttGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCaa          >  2:1645455/1‑101 (MQ=255)
                                                                                           tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         >  1:1576194/1‑101 (MQ=255)
                                                                                           tGGAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAg         <  1:1084044/101‑1 (MQ=255)
                                                                                            ggAGCAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGc        <  1:2020934/101‑1 (MQ=255)
                                                                                                cAAGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTc    >  2:2936765/1‑101 (MQ=255)
                                                                                                  aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  1:2881611/101‑1 (MQ=255)
                                                                                                  aGATG.AAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCg  <  1:495790/101‑1 (MQ=255)
                                                                                                       |                                                                                                
AGGCGTGTCAACCGTGGCGC.TTTTGCGCAGGCAAAA.GGGGGGCGCTGCCCCCCTCTTGG.CTTC.GGCCAATTCGCCCCCCGAGGTATTTGGAACAAGATGAAAAGACCATGGGTTCATCTTGCCTCAAATATCCCGGGGGGAGTCCTGAAAGGACGGGGGGCAGAGCCCCCCTCCCGGGTCTTTCTCAAGCGTTCCG  >  NC_014034/2640102‑2640297

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG