Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,024,766 Δ1 bp 100% intergenic (‑176/‑152) chvI ← / → pckA transcriptional regulator ChvI/phosphoenolpyruvate carboxykinase

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,024,7660T→.100.0% 1231.1 280intergenic (‑176/‑152)chvI/pckAtranscriptional regulator ChvI/phosphoenolpyruvate carboxykinase
Reads supporting (aligned to +/- strand):  new base (152/126):  ref base (0/1):  total (153/127)

Only 100 of 307 total aligned reads displayed.

GCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024667‑3024862
                                                                                                   |                                                                                                
gCGAATCGCCGACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtt                                                                                                 <  1:2499783/101‑1 (MQ=255)
 ctaaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  2:3959133/99‑1 (MQ=255)
 cGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  1:4453304/101‑1 (MQ=255)
 cGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                >  2:1876766/1‑101 (MQ=255)
 cGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCCCCCCTCTTCGCGATGATGGCGCTAAATCGGCCCTGttt                                                                                                <  1:3367952/101‑1 (MQ=255)
  gAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttg                                                                                               <  2:2416874/101‑2 (MQ=255)
   aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttgc                                                                                              <  2:1626339/101‑3 (MQ=255)
   aaTCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGtttag                                                                                              >  1:3959425/1‑99 (MQ=255)
       gCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTa                                                                                         <  2:732043/101‑1 (MQ=255)
          cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      >  2:2171727/1‑101 (MQ=255)
          cACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGAGGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCt                                                                                      >  2:667698/1‑101 (MQ=255)
            cGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGc                                                                                    <  1:1577092/101‑1 (MQ=255)
              tGATGCGAATCATCTGAGCGC.AAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 <  2:3746972/101‑1 (MQ=255)
               gATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 <  2:4678268/101‑1 (MQ=255)
               gATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGAc                                                                                 >  2:2224606/1‑101 (MQ=255)
                aTGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACa                                                                                <  1:756125/101‑1 (MQ=255)
                aTGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACa                                                                                >  2:3405308/1‑101 (MQ=255)
                 tGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaa                                                                               <  2:4731562/101‑1 (MQ=255)
                  gCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaaa                                                                              <  1:4677210/101‑1 (MQ=255)
                  gCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACaaa                                                                              <  2:2682725/101‑1 (MQ=255)
                  gCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCACTG.TTTGCGCTAGCTGCGACaaa                                                                              <  1:1057684/101‑1 (MQ=255)
                      atcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCt                                                                          <  1:2902988/101‑1 (MQ=255)
                       tcatcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCtt                                                                         <  1:3825148/101‑1 (MQ=255)
                        catcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTc                                                                        <  2:4456400/101‑1 (MQ=255)
                          tcTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTTGCGATGATGGCGCTAAATAGGCACTG.ATTGCGCTAGCTGCGACACACCCTTCTg                                                                      >  2:3183714/1‑101 (MQ=255)
                           cTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGt                                                                     >  1:3746640/1‑101 (MQ=255)
                             gAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTc                                                                   >  2:2973337/1‑101 (MQ=255)
                             gAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTc                                                                   <  1:4918404/101‑1 (MQ=255)
                              aGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTcc                                                                  >  2:2130075/1‑101 (MQ=255)
                                  cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              <  1:2576809/101‑1 (MQ=255)
                                  cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              <  1:232245/101‑1 (MQ=255)
                                  cAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCg                                                              >  1:2279951/1‑101 (MQ=255)
                                   aaaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACACTTCTGTTCCTTCGc                                                             <  1:3060849/101‑1 (MQ=255)
                                    aaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCa                                                            >  2:3156646/1‑101 (MQ=255)
                                    aaaaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCa                                                            <  1:1217923/101‑1 (MQ=255)
                                      aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                          >  2:1063867/1‑101 (MQ=255)
                                      aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                          >  1:889881/1‑101 (MQ=255)
                                      aaaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAt                                                          <  1:2199373/101‑1 (MQ=255)
                                       aaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         >  1:171619/1‑101 (MQ=255)
                                       aaaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAAtt                                                         <  1:2026057/101‑1 (MQ=255)
                                        aaCGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTg                                                        <  2:2320529/101‑1 (MQ=255)
                                           gAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTgcgc                                                     >  1:4789272/1‑101 (MQ=255)
                                            aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGcc                                                    >  2:4980840/1‑101 (MQ=255)
                                            aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGcc                                                    >  2:4266206/1‑101 (MQ=255)
                                            aaGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGcc                                                    >  2:1069491/1‑101 (MQ=255)
                                             aGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCa                                                   >  1:4426817/1‑101 (MQ=255)
                                              ggTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCAt                                                  <  2:3280443/101‑1 (MQ=255)
                                               gTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATg                                                 <  2:5024609/101‑1 (MQ=255)
                                                tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                >  2:88375/1‑101 (MQ=255)
                                                tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                >  2:1537938/1‑101 (MQ=255)
                                                tttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATgg                                                <  2:1382857/101‑1 (MQ=255)
                                                 ttGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGt                                               >  1:4013487/1‑101 (MQ=255)
                                                  tGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGta                                              >  2:3294045/1‑101 (MQ=255)
                                                   gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             >  1:3002752/1‑101 (MQ=255)
                                                   gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  1:3113661/101‑1 (MQ=255)
                                                   gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  2:3065877/101‑1 (MQ=255)
                                                   gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             >  1:1648914/1‑101 (MQ=255)
                                                   gACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGtat                                             <  2:3620443/101‑1 (MQ=255)
                                                      tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  1:284684/1‑101 (MQ=255)
                                                      tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          <  1:4283897/101‑1 (MQ=255)
                                                      tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          <  1:4981170/101‑1 (MQ=255)
                                                      tAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgg                                          >  1:4589958/1‑101 (MQ=255)
                                                       aaTTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAggg                                         <  2:3921872/101‑1 (MQ=255)
                                                        aTTTGTCTCACCTCTTCGCTATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgc                                        <  2:1469235/101‑1 (MQ=255)
                                                         tttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAgggag                                       >  2:3603179/1‑99 (MQ=255)
                                                          ttGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGgcgc                                      >  2:4632738/1‑101 (MQ=255)
                                                           tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     >  1:2115573/1‑101 (MQ=255)
                                                           tGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGcc                                     >  2:1238297/1‑101 (MQ=255)
                                                             tctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCAt                                   <  1:4836576/101‑1 (MQ=255)
                                                             tctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCAt                                   >  2:4811720/1‑101 (MQ=255)
                                                             tctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCAt                                   >  1:1393897/1‑101 (MQ=255)
                                                              ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                  <  2:4325706/101‑1 (MQ=255)
                                                              ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                  >  2:1823250/1‑101 (MQ=255)
                                                              ctcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATc                                  >  2:3477854/1‑101 (MQ=255)
                                                               tcACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATcc                                 <  2:4894458/101‑1 (MQ=255)
                                                                cACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  2:1713967/1‑101 (MQ=255)
                                                                cACCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCg                                >  1:730460/1‑101 (MQ=255)
                                                                 aCCTCTTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGc                               >  1:589827/1‑101 (MQ=255)
                                                                    tctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAAc                            <  2:495823/101‑1 (MQ=255)
                                                                    tctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAAc                            <  2:2084848/101‑1 (MQ=255)
                                                                     ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           >  1:4172442/1‑101 (MQ=255)
                                                                     ctTCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           >  2:261721/1‑101 (MQ=255)
                                                                     ctTCGCGATGATGGCGCTAAATCGGCCCTG.TGTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACa                           <  1:4031169/101‑1 (MQ=255)
                                                                       tCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAtt                         <  2:401948/101‑1 (MQ=255)
                                                                       tCGCGATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACAtt                         >  1:2778128/1‑101 (MQ=255)
                                                                        cgcgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTc                        <  1:3453691/101‑1 (MQ=255)
                                                                        cgcgATGATGGCGCTAAATCAGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTc                        >  2:2000181/1‑101 (MQ=255)
                                                                         gcgATGATGGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCt                       >  1:793114/1‑101 (MQ=255)
                                                                            atgatgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTgc                    <  2:1579410/101‑1 (MQ=255)
                                                                               atgGCGCTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGcgcg                 <  1:3605459/101‑1 (MQ=255)
                                                                                   cgcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATc             >  1:3258168/1‑101 (MQ=255)
                                                                                    gcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCg            <  2:1038453/101‑1 (MQ=255)
                                                                                    gcTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCg            <  1:1787229/101‑1 (MQ=255)
                                                                                     cTAAATCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCgg           >  1:3652179/1‑101 (MQ=255)
                                                                                       aaaTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTg         >  1:1929901/1‑101 (MQ=255)
                                                                                         aTCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcc       <  1:1823383/101‑1 (MQ=255)
                                                                                          tCGGCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgccc      <  2:1278412/101‑1 (MQ=255)
                                                                                             gCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc   <  1:2164164/101‑1 (MQ=255)
                                                                                             gCCCTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgcc   >  1:3564759/1‑101 (MQ=255)
                                                                                              cccTG.TTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTgcccgccc  <  2:2819189/101‑1 (MQ=255)
                                                                                                   |                                                                                                
GCGAATCGCCCACGTGATGCGAATCATCTGAGCGCAAAAAAACGAAGGTTTGACTAATTTGTCTCACCTCTTCGCGATGATGGCGCTAAATCGGCCCTGTTTTGCGCTAGCTGCGACAAACCCTTCTGTTCCTTCGCAATTGCGCCATGGTATAGGGCGCCATCCGCAACATTCTTGCGCGGATCGGTGCCCGCCC  >  NC_014034/3024667‑3024862

Base quality scores:  ATCG  < 3 ≤  ATCG  < 25 ≤  ATCG  < 31 ≤  ATCG  < 35 ≤  ATCG  < 41 ≤  ATCG