Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,254,687 Δ1 bp 100% intergenic (+12/‑171) glpX → / → recJ fructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,254,6870G→.100.0% 54.2 123intergenic (+12/‑171)glpX/recJfructose‑1,6‑bisphosphatase/single‑stranded‑DNA‑specific exonuclease RecJ
Reads supporting (aligned to +/- strand):  new base (2/103):  ref base (17/0):  total (20/103)
Fisher's exact test strand distribution p-value = 6.66e-19
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 5.01e-10

Only 100 of 169 total aligned reads displayed.

ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTG  >  NC_014034/3254587‑3254780
                                                                                                     |                                                                                                    
aCCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACg                                                                                                      <  2:4241024/101‑1 (MQ=255)
    gAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggg                                                                                                  <  1:2388307/101‑1 (MQ=255)
     aaaTTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggg                                                                                                 >  2:3625671/1‑101 (MQ=255)
        ttCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactg                                                                                              <  1:3163853/101‑5 (MQ=255)
         tCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggggg                                                                                              >  1:1078642/1‑98 (MQ=255)
          cATCGAGACCGAGACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggggg                                                                                             >  1:2738621/1‑97 (MQ=255)
          cATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgcc                                                                                            <  2:3597733/101‑7 (MQ=255)
           aTCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACggggggccggcg                                                                                           >  2:4151534/1‑95 (MQ=255)
           aTCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccg                                                                                           <  2:3447618/101‑8 (MQ=255)
            tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggggcgg                                                                                           >  2:4202218/1‑95 (MQ=255)
            tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCggggggggccgc                                                                                           >  2:2622236/1‑95 (MQ=255)
            tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCaggggggggggg                                                                                           >  2:1855648/1‑95 (MQ=255)
            tCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.ATGc.ccg                                                                                        >  2:1595029/1‑99 (MQ=255)
             cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggggggccctc                                                                                         >  1:1392850/1‑93 (MQ=255)
             cGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgtc                                                                                         <  1:748143/101‑10 (MQ=255)
              gagaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACgggggactgccgtcc                                                                                        >  1:863665/1‑91 (MQ=255)
               agaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggggcggccc                                                                                        >  1:2716906/1‑92 (MQ=255)
                gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tcccc                                                                                   <  2:3744939/101‑1 (MQ=255)
                gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tcccc                                                                                   <  2:1101497/101‑1 (MQ=255)
                gaCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGGGATCTATCGCAACCCGATCCGCTGAGGCAACGCTACCgggggggggccggccc                                                                                       >  1:2595977/1‑91 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACCGGGGG.CGGC.CG.Tccccc                                                                                  >  2:2000155/1‑101 (MQ=255)
                 aCCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.Tccccc                                                                                  <  1:4123047/101‑1 (MQ=255)
                   cGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGACTG..CG.GCCCCCGc                                                                                >  2:4131950/1‑101 (MQ=255)
                      aaCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCAcc                                                                             <  2:1203357/101‑1 (MQ=255)
                       aCGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCAccc                                                                            <  2:4943824/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCt                                                                         <  2:3536668/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCt                                                                         <  2:2200959/101‑1 (MQ=255)
                          aTCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATACGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.CCCCCCCCCcccccc                                                                         >  1:2413243/1‑100 (MQ=255)
                           accTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTg                                                                        <  1:1139185/100‑1 (MQ=255)
                            ccTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACACGGGG.CTGCGCG.TCCCCCCCCccccccc                                                                        >  1:300974/1‑99 (MQ=255)
                             cTGATGCTCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcg                                                                      <  2:5006776/101‑1 (MQ=255)
                             cTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcg                                                                      <  2:1225474/101‑1 (MQ=255)
                             cTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcg                                                                      <  2:1225473/101‑1 (MQ=255)
                              tGATGCGCCCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                                     <  1:4511704/101‑1 (MQ=255)
                              ggATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTgcgc                                                                     <  1:984804/100‑1 (MQ=255)
                               gATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.CG.CCCCCCCCCcccccccccc                                                                     >  1:3883461/1‑96 (MQ=255)
                                aTGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGt                                                                   <  1:2511198/101‑1 (MQ=255)
                                aTGCGCCCGAAACCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGt                                                                   <  2:1761916/101‑1 (MQ=255)
                                 tGCGCTCGAAACCCGACTCGGTGCGCCGCGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACACCCTGCGCGTa                                                                  <  2:3380724/101‑1 (MQ=255)
                                 tGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTa                                                                  <  1:3802542/101‑1 (MQ=255)
                                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.GG.CCCCCCCCCcccccccgcgcgt                                                                  >  2:606304/1‑93 (MQ=255)
                                  gcgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.t                                                                <  1:4102046/101‑1 (MQ=255)
                                   cgcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.tt                                                               <  2:3757239/101‑1 (MQ=255)
                                    tcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                              <  2:113710/100‑1 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                              <  1:2454896/101‑1 (MQ=255)
                                    gcTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.ttt                                                              <  2:1502133/101‑1 (MQ=255)
                                     cTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.tttt                                                             <  1:3383800/101‑1 (MQ=255)
                                      ccgaaccccGGCTCGGTGGGGCGGGGGATCTATCGCAGCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTg                                                            <  2:2678738/94‑1 (MQ=255)
                                       cGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGAATGC.CG.TCCCCCCCACCCCCTTCGCGTT.tttttt                                                           >  2:1690751/1‑99 (MQ=255)
                                        gAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGcc                                                          <  1:24869/101‑1 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GCGC.CC.CCCCCCGCCCCCCCCCCGCGCG.ttttttca                                                         >  2:154999/1‑97 (MQ=255)
                                         aaaaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCa                                                         <  2:3264212/101‑1 (MQ=255)
                                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAg                                                       <  1:2363306/101‑1 (MQ=255)
                                           aaCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAg                                                       <  1:4973651/101‑1 (MQ=255)
                                            aCCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAgg                                                      <  2:4495184/101‑1 (MQ=39)
                                             ccGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.a                                                    <  1:4912631/101‑1 (MQ=39)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCCCCCCCCCCCCGCGGA.TTTTGCCCAGG.aaa                                                  >  2:4874010/1‑101 (MQ=255)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.aaa                                                  <  1:803773/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.aaa                                                  <  2:52710/101‑1 (MQ=38)
                                               ggCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.aaa                                                  <  2:4354334/101‑1 (MQ=38)
                                                  tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACg                                               <  2:3294069/101‑1 (MQ=38)
                                                  tCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACg                                               <  1:3459689/101‑1 (MQ=38)
                                                   cGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACgg                                              <  2:3788043/101‑1 (MQ=38)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.GAAACggg                                             >  2:4686994/1‑101 (MQ=38)
                                                    ggTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCAACCCCTGCGCGTA.TTTTGCCAAGG.AAAACggg                                             <  2:3428920/101‑1 (MQ=38)
                                                     gTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCGCCCCCCCTGCGCGGA.TTTTGCCAAGG.aaaaacggg                                            >  2:2428698/1‑96 (MQ=37)
                                                        cgcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCg                                         <  1:1186500/101‑1 (MQ=38)
                                                         gcCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.GCCCCCCCACCCCCCGCGCGGA.TTTTGCCCAGGAAAAACGGGGcgg                                         >  2:1351487/1‑99 (MQ=255)
                                                          ccGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGGGGGGC.GGCCCCCCCCCCCCCCCCGCGCGT..TTTTTCCAGGA.AAAAAGGGGcggg                                        >  1:3701196/1‑98 (MQ=255)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                     <  1:2134276/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                     <  1:4681595/101‑1 (MQ=38)
                                                            gggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTc                                     <  1:35759/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                    <  1:1336623/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                    <  2:2651208/101‑1 (MQ=38)
                                                             ggTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCa                                    <  2:2357809/101‑1 (MQ=38)
                                                               tGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCAcc                                  <  1:606383/101‑1 (MQ=38)
                                                                gATCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CG.TCCCCCCCACCCCCCCCCCCGA.TTTTTCCCAGG.AAAAAGGGGGCGGTTGAccc                                 >  1:3014124/1‑100 (MQ=255)
                                                                  tCTATCGCAACCCGATCCGCTGAGGCAACGCT.ACGGGGGG.CTGC.CG.TCCCCCCCACCCCCCGCGCGTATTTTTCCCAGGG.AAAACGGGG.CGTTTCACCCTg                               >  2:4795371/1‑101 (MQ=255)
                                                                   cTATCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTgg                              <  1:1855781/101‑1 (MQ=38)
                                                                     aTCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCCCCCCTTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGc..c                          <  1:1661387/101‑1 (MQ=255)
                                                                      tCGCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..Cg                         <  2:40796/101‑1 (MQ=38)
                                                                        gCAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGaa                       <  1:4940112/101‑1 (MQ=38)
                                                                         cAACCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGaaa                      <  1:716970/101‑1 (MQ=38)
                                                                          aaCCCGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAat                     <  2:2513724/101‑1 (MQ=38)
                                                                            cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAatat                   <  2:775468/101‑1 (MQ=38)
                                                                            cccGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAatat                   <  1:4595005/101‑1 (MQ=38)
                                                                             ccGATCCGCTGAGGCAACGCTACGGGGGGGGGCCC.CC.CCCCCCCCCCCCCCCCCGCGT..TTTTTCCAAGA.AAAAAGGGGCGGGTTTCCCTTGGcgcaaaatc                     >  1:2977942/1‑93 (MQ=255)
                                                                              cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.cc                <  1:5029297/101‑1 (MQ=38)
                                                                              cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.cc                <  2:4636215/101‑1 (MQ=38)
                                                                              cGATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.cc                <  2:4882179/101‑1 (MQ=38)
                                                                               gATCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.ccc               <  1:4585089/101‑1 (MQ=38)
                                                                                aTCCGCTGAGGCAACGCT.ACGGGGGG.GGGC.CGCCCCCCCCCCCCCCCCCCCCCCT.TTTTTCCAAGA.AAAAAGGGGCCGTTTCCCCTTGCC..CGAAAAAC.ccc               >  1:504632/1‑101 (MQ=255)
                                                                                 tCCGCTGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCgg             <  2:3165245/101‑1 (MQ=38)
                                                                                      tGAGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCggggggg        <  1:4588626/101‑1 (MQ=38)
                                                                                       gAGGCAACGCT.AC.GGGGGACGGC.CG.CCCCCCGCACCCCCTGCGCGGC.TTTTTGCAAGG.AAAACAGGGGCGGTTCTCCTCGGCTGCGAAATA..CCAggggggg        >  2:3364709/1‑101 (MQ=255)
                                                                                        aGGCAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGaa      <  2:278420/101‑1 (MQ=38)
                                                                                           cAACGCT.ACGGGGGG.CTGC.CG.GCCCCCCCCCCCCCCCCGCGGA.TTTTTCCAAGG.AAAACGGGGCCGGTTCACCTTTGG..CGCAAAATCCCCGGGGGGGAAtt    >  2:4565034/1‑101 (MQ=255)
                                                                                           cAACGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAAttt   <  1:3816169/101‑1 (MQ=38)
                                                                                            aaCGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTg  <  1:188463/101‑1 (MQ=38)
                                                                                            aaCGCT.AC.GGGGGACTGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTg  <  1:4687317/101‑1 (MQ=38)
                                                                                                     |                                                                                                    
ACCCGAAATTCATCGAGACCGAAACGATCCTGATGCGCTCGAAAACCGGCTCGGTGCGCCGGGTGATCTATCGCAACCCGATCCGCTGAGGCAACGCT.CCGGGGGGTATGC.CG.TCCCCCGCACCCCCTGCGCGTA.TTTTGCCAAGG.AAAACGGGGCCGTTTCACCTTGGC..CGAAATAT.CCCGGGGGGGAATTTG  >  NC_014034/3254587‑3254780

Base quality scores:  ATCG  < 3 ≤  ATCG  < 18 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG