Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,385 Δ1 bp 90.4% intergenic (‑95/+35) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3850C→.90.4% 26.6 ‑1.2 ‑0.5104intergenic (‑95/+35)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (37/57):  ref base (6/4):  total (43/61)
Fisher's exact test strand distribution p-value = 3.12e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 3.32e-01

Only 100 of 110 total aligned reads displayed.

CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCG  >  NC_014034/3461307‑3461460
                                                                                |                                                                            
cGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCGCGCCCc.ccaccccgg                                                       >  2:2270533/1‑97 (MQ=255)
   ccccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                <  2:4323307/100‑1 (MQ=255)
   aCCCGCAGCTTAGCAGGATGCGCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                >  1:2865036/1‑101 (MQ=255)
      cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  1:3725713/101‑1 (MQ=255)
      cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  2:3880897/101‑1 (MQ=255)
      cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                             <  1:2722614/101‑1 (MQ=255)
       gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                            >  2:1769283/1‑101 (MQ=255)
        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                           >  1:1141990/1‑101 (MQ=255)
        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                           >  2:1631519/1‑101 (MQ=255)
         agtgtAGCAGGAGGCAGGGGCGGTTGCAGACGAAGTTTGGCTCAAGGCCAAAGGAA.GG.GG.GCA..CG..GCCACCCGGCCGCGCCCCAAACCCCGGTCGTTCGAt                                          <  2:3521594/97‑1 (MQ=255)
         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                          <  1:4779826/101‑1 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  2:250744/1‑101 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  1:591327/1‑101 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  1:1653085/1‑101 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         <  2:142511/101‑1 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                         >  2:1361351/1‑101 (MQ=255)
           cTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                        >  1:3617997/1‑101 (MQ=255)
           cTTAGAAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCa                                        >  2:1855714/1‑101 (MQ=255)
            ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                       >  1:2141290/1‑101 (MQ=255)
            ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                       >  2:3886149/1‑101 (MQ=255)
            ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                       >  2:3549301/1‑101 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  2:428730/101‑1 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      <  1:448840/101‑1 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      >  1:4125168/1‑101 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                      >  2:3011682/1‑101 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:689429/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  1:1276818/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:5034921/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                   <  2:3163697/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGCACGTTCGATCAGTTCt                                   >  2:2290721/1‑101 (MQ=255)
                 aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                  <  2:4563316/101‑1 (MQ=255)
                 aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                  <  1:3578248/101‑1 (MQ=255)
                  ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                 <  1:4087209/101‑1 (MQ=255)
                  ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                 >  1:936294/1‑101 (MQ=255)
                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                <  2:2631613/101‑1 (MQ=255)
                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                <  2:497460/101‑1 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                               <  1:3823998/101‑1 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                               <  2:1138371/101‑1 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                              <  2:3653283/101‑1 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                              <  1:4738923/101‑1 (MQ=255)
                      gCAGGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAATGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcga                             <  1:4016918/101‑1 (MQ=255)
                      gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTCCGATCAGTTCTTGCcga                             >  1:2244907/1‑101 (MQ=255)
                       cACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgac                            <  1:4916825/101‑1 (MQ=255)
                         cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacga                          <  1:2747186/101‑1 (MQ=255)
                          gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                         <  2:399491/101‑1 (MQ=255)
                           ggCGCGGCCAGACGCAGTTTGGTGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                        >  1:2047346/1‑101 (MQ=255)
                           ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                        <  1:1123647/101‑1 (MQ=255)
                            gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCTCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                       <  1:2442575/101‑1 (MQ=255)
                            gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                       >  1:4296095/1‑101 (MQ=255)
                            gcgcGGCCAGACGCAGTTGGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                       <  1:44086/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  2:527833/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      >  1:1688656/1‑101 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  1:474483/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                      <  2:4482703/101‑1 (MQ=255)
                              gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                     <  2:3618306/101‑1 (MQ=255)
                               cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    >  2:811604/1‑101 (MQ=255)
                               cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                    <  2:4577751/101‑1 (MQ=255)
                                ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                   <  1:2139702/101‑1 (MQ=255)
                                 gcaaGACGCGGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCACCCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  <  2:2275964/98‑1 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  1:3224964/1‑101 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:3094601/1‑101 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:2904786/1‑101 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                  >  2:2728934/1‑101 (MQ=255)
                                  cctacctcAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  1:3557390/94‑1 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  1:4579038/101‑1 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  2:1962496/101‑1 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                 <  2:1621888/101‑1 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               >  2:2783727/1‑101 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               <  1:2759718/101‑1 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag               >  2:136277/1‑101 (MQ=255)
                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg              >  2:941907/1‑101 (MQ=255)
                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg              <  2:3238126/101‑1 (MQ=255)
                                       cGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc            >  2:4475640/1‑101 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  1:721208/101‑1 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           >  2:3639907/1‑101 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt           <  2:4088210/101‑1 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGCCGTTCGATCAGTTCTTGCCAACGATCACGTCAGAGACCt           >  2:3543420/1‑101 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagagatcgg          >  2:3557120/1‑96 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt          <  2:3431921/101‑1 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTGGCCGACGATGACGTCAGAGGCCtt          >  2:4524917/1‑101 (MQ=255)
                                          attttGGCGGAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCGCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:3072808/99‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  1:279810/101‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:819996/101‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         <  2:4202124/101‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg         >  1:2330891/1‑101 (MQ=255)
                                           gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga        <  1:2103259/101‑1 (MQ=255)
                                            tttGGCGGAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat       <  2:4895966/101‑1 (MQ=255)
                                            tttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat       <  1:838793/101‑1 (MQ=255)
                                            tttGGCGCAGGGCCAAAGG.ACGG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat       <  1:3233178/101‑1 (MQ=255)
                                             ttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg      <  1:967084/101‑1 (MQ=255)
                                             ttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg      >  2:2005049/1‑101 (MQ=255)
                                              tGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga     >  1:249448/1‑101 (MQ=255)
                                              tGGCGCAGGGCCAAAGAAAGGGAGC.GCAGAC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga     <  2:836350/101‑1 (MQ=255)
                                               ggCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  2:2984449/1‑101 (MQ=255)
                                               ggCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat    >  2:3128474/1‑101 (MQ=255)
                                                gcgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc   >  2:716085/1‑101 (MQ=255)
                                                 cgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  >  1:287405/1‑101 (MQ=255)
                                                 cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  2:2244752/101‑1 (MQ=255)
                                                 cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  1:4806283/101‑1 (MQ=255)
                                                 cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg  <  1:2818690/101‑1 (MQ=255)
                                                                                |                                                                            
CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCG  >  NC_014034/3461307‑3461460

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 34 ≤  ATCG  < 41 ≤  ATCG