Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 3,461,393 Δ1 bp 4.9% intergenic (‑103/+27) RCAP_rcc03260 ← / ← RCAP_rcc03261 NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_0140343,461,3930G→. 4.9% 11.7 ‑2.4 ‑0.0122intergenic (‑103/+27)RCAP_rcc03260/RCAP_rcc03261NAD‑dependent epimerase/dehydratase/TOBE domain‑containing protein
Reads supporting (aligned to +/- strand):  new base (5/1):  ref base (49/66):  total (55/67)
Fisher's exact test strand distribution p-value = 8.78e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.99e-01

Only 100 of 137 total aligned reads displayed.

CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGA  >  NC_014034/3461307‑3461492
                                                                                        |                                                                                                    
   ccccGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                                                <  2:4323307/100‑1 (MQ=255)
   aCCCGCAGCTTAGCAGGATGCGCGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCg                                                                                >  1:2865036/1‑101 (MQ=255)
      cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                             <  1:2722614/101‑1 (MQ=255)
      cGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTc                                                                             <  2:3880897/101‑1 (MQ=255)
       gCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCg                                                                            >  2:1769283/1‑101 (MQ=255)
        cAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGa                                                                           >  1:1141990/1‑101 (MQ=255)
         agtgtAGCAGGAGGCAGGGGCGGTTGCAGACGAAGTTTGGCTCAAGGCCAAAGGAA.GG.GG.GCA..CG..GCCACCCGGCCGCGCCCCAAACCCCGGTCGTTCGAt                                                                          <  2:3521594/97‑1 (MQ=255)
         aGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGAt                                                                          <  1:4779826/101‑1 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                         >  2:1361351/1‑101 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                         >  1:591327/1‑101 (MQ=255)
          gCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATc                                                                         >  1:1653085/1‑101 (MQ=255)
            ttAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAg                                                                       >  2:3549301/1‑101 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      <  2:1734556/101‑1 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      <  2:428730/101‑1 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  2:3011682/1‑101 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      <  1:448840/101‑1 (MQ=255)
             tAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGt                                                                      >  1:4125168/1‑101 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                                   <  2:3163697/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                                   <  2:689429/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCt                                                                   <  2:5034921/101‑1 (MQ=255)
                cAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGCACGTTCGATCAGTTCt                                                                   >  2:2290721/1‑101 (MQ=255)
                 aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                                  <  2:3906992/101‑1 (MQ=255)
                 aGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCtt                                                                  <  1:3578248/101‑1 (MQ=255)
                  ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                                                 <  1:4087209/101‑1 (MQ=255)
                  ggATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTg                                                                 >  1:936294/1‑101 (MQ=255)
                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                                <  2:497460/101‑1 (MQ=255)
                   gATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGc                                                                <  2:2631613/101‑1 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                               <  2:1138371/101‑1 (MQ=255)
                    aTGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGcc                                                               <  1:3823998/101‑1 (MQ=255)
                     tGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcg                                                              <  2:3653283/101‑1 (MQ=255)
                      gCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTCCGATCAGTTCTTGCcga                                                             >  1:2244907/1‑101 (MQ=255)
                         cGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCcgacga                                                          <  1:2747186/101‑1 (MQ=255)
                          gggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGAt                                                         <  2:399491/101‑1 (MQ=255)
                           ggCGCGGCCAGACGCAGTTTGGTGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                        >  1:2047346/1‑101 (MQ=255)
                           ggCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATg                                                        <  1:1123647/101‑1 (MQ=255)
                            gcgcGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCTCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                       <  1:2442575/101‑1 (MQ=255)
                            gcgcGGCCAGACGCAGTTGGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGa                                                       <  1:44086/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                      <  1:3113334/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                      <  1:474483/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                      <  2:4482703/101‑1 (MQ=255)
                             cgcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGAc                                                      >  1:1688656/1‑101 (MQ=255)
                              gcgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACg                                                     <  2:3618306/101‑1 (MQ=255)
                               cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                                                    >  2:811604/1‑101 (MQ=255)
                               cgGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGt                                                    <  2:4577751/101‑1 (MQ=255)
                                ggCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTc                                                   <  1:2139702/101‑1 (MQ=255)
                                 gcaaGACGCGGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG.CCCC.CC.GGCCGCGCCCCCACCCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                                  <  2:2275964/98‑1 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                                  >  2:3094601/1‑101 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                                  >  2:2904786/1‑101 (MQ=255)
                                 gCCAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCa                                                  >  1:3224964/1‑101 (MQ=255)
                                  cctacctcAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 <  1:3557390/94‑1 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 <  1:4579038/101‑1 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 <  2:1621888/101‑1 (MQ=255)
                                  ccAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCag                                                 <  2:1962496/101‑1 (MQ=255)
                                   cAGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCaga                                                <  1:4166601/101‑1 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               <  1:2759718/101‑1 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               >  2:136277/1‑101 (MQ=255)
                                    aGACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagag                                               >  2:2783727/1‑101 (MQ=255)
                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                              <  2:3238126/101‑1 (MQ=255)
                                     gACGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAgg                                              >  2:941907/1‑101 (MQ=255)
                                       cGCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGcc                                            >  2:4475640/1‑101 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                           >  2:3639907/1‑101 (MQ=255)
                                        gCAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCt                                           <  2:2697081/101‑1 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCagagatcgg                                          >  2:3557120/1‑96 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCtt                                          <  2:3431921/101‑1 (MQ=255)
                                         cAGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTGGCCGACGATGACGTCAGAGGCCtt                                          >  2:4524917/1‑101 (MQ=255)
                                          attttGGCGGAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCGCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         <  2:3072808/99‑1 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         >  1:2330891/1‑101 (MQ=255)
                                          aGTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtg                                         <  2:4202124/101‑1 (MQ=255)
                                           gTTTGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtga                                        <  1:2103259/101‑1 (MQ=255)
                                            tttGGCGGAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       <  2:4895966/101‑1 (MQ=255)
                                            tttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgat                                       <  1:838793/101‑1 (MQ=255)
                                             ttGGCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatg                                      >  2:2005049/1‑101 (MQ=255)
                                              tGGCGCAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     >  1:249448/1‑101 (MQ=255)
                                              tGGCGCAGGGCCAAAGAAAGGGAGC.GCAGAC.ACCCCGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTtgatga                                     <  2:836350/101‑1 (MQ=255)
                                               ggCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                                    >  2:4618039/1‑101 (MQ=255)
                                               ggCGCAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTgatgat                                    >  1:609573/1‑101 (MQ=255)
                                                gcgcAGGGCCAAAGGAAGGG.GGCGCAGCC.ACCCGGCCG...CG..CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATc                                   >  2:716085/1‑101 (MQ=255)
                                                 cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                                  <  1:4806283/101‑1 (MQ=255)
                                                 cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                                  <  2:2244752/101‑1 (MQ=255)
                                                 cgcAGGGCCAAAGGAA.GG.GG.GC.GCAG...CCACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATcg                                  <  1:2818690/101‑1 (MQ=255)
                                                                         ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCTGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg                 >  1:4297634/10‑101 (MQ=255)
                                                                         ggggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCg                 <  1:328112/92‑1 (MQ=255)
                                                                          gggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGa                >  2:1588859/9‑101 (MQ=255)
                                                                           ggcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAc               <  1:1645384/94‑1 (MQ=255)
                                                                            gcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAcc              >  2:2283399/7‑101 (MQ=255)
                                                                            gcgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGAcc              >  2:1483295/7‑101 (MQ=255)
                                                                             cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt             >  2:3301305/6‑101 (MQ=255)
                                                                             cgcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCt             >  1:4629558/6‑101 (MQ=255)
                                                                              gcagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGACGATCGCCCAGGCCTCGCCGCCGACCtt            >  1:3694834/5‑101 (MQ=255)
                                                                               cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc           <  1:392578/98‑1 (MQ=255)
                                                                               cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc           >  1:2218640/4‑101 (MQ=255)
                                                                               cagccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTc           >  1:3334709/4‑101 (MQ=255)
                                                                                  ccACCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgc        <  1:880824/101‑1 (MQ=255)
                                                                                    aCCCGGCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAgcgc      >  1:3588531/1‑101 (MQ=255)
                                                                                     cccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGcc     <  2:2757776/98‑1 (MQ=255)
                                                                                     cccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGcc     >  1:376512/4‑101 (MQ=255)
                                                                                     cccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGcc     <  1:3094839/98‑1 (MQ=255)
                                                                                      ccggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCa    <  1:3278547/99‑1 (MQ=255)
                                                                                        ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  >  1:1186434/1‑101 (MQ=255)
                                                                                        ggCCGCGCCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGa  <  1:3652293/101‑1 (MQ=255)
                                                                                        |                                                                                                    
CGCACCCGCAGCTTAGCAGGATGCACGGGCGCGGCCAGACGCAGTTTGGCGCAGGGCCAAAGGAAGGG.GG.GCAGCCGACCCCACCGGGCCGC.CCCCCAACCCCGGTCGTTCGATCAGTTCTTGCCGACGATGACGTCAGAGGCCTTGATGATCGCGCAGGCCTCGTCGCCGACCTTCAGCGCCAGA  >  NC_014034/3461307‑3461492

Base quality scores:  ATCG  < 3 ≤  ATCG  < 20 ≤  ATCG  < 29 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG