Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,432 Δ1 bp 2.0% intergenic (+35/+113) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4320A→. 2.0% 3.7 ‑2.9 ‑1.0198intergenic (+35/+113)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (49/145):  total (52/146)
Fisher's exact test strand distribution p-value = 5.63e-02
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 1.01e-01

Only 100 of 219 total aligned reads displayed.

CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGG  >  NC_014034/432333‑432532
                                                                                                     |                                                                                                     
 ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGAc                                                                                                      <  2:2289827/101‑1 (MQ=255)
 ccGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGAc                                                                                                      <  2:195533/101‑1 (MQ=255)
  cGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGGCt                                                                                                     >  2:1169527/1‑101 (MQ=255)
      cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTcccc                                                                                                 <  2:3668232/101‑1 (MQ=255)
      cgcTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTcccc                                                                                                 >  1:1544049/1‑101 (MQ=255)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTcccccc                                                                                               <  2:454604/101‑1 (MQ=255)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTcccccc                                                                                               <  2:2531365/101‑1 (MQ=255)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTcccccc                                                                                               <  1:1803397/101‑1 (MQ=255)
        cTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCCGCCGACTcccccc                                                                                               >  1:3302411/1‑101 (MQ=38)
         tGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCg                                                                                              <  2:2419290/101‑1 (MQ=255)
           ggCAGGATGGGGATCGATCCGCGCACGCTTGCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCggg                                                                                            <  1:4592521/101‑1 (MQ=25)
           ggCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCggg                                                                                            <  2:1367735/101‑1 (MQ=255)
             cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGat                                                                                          <  1:570398/101‑1 (MQ=255)
             cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGat                                                                                          <  1:1940700/101‑1 (MQ=255)
             cACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGat                                                                                          <  1:1042097/101‑1 (MQ=255)
              gcGATNGGGATCGACCCGCGCACGCTTTGGGCGGCCGCGGGCCACGCCTGAGACCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGata                                                                                         <  1:911443/100‑1 (MQ=21)
               cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTccccccccgccaa                                                                                        >  1:114464/1‑94 (MQ=37)
               cGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGatat                                                                                        <  2:1532565/101‑1 (MQ=255)
               cGATGGGAATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGatat                                                                                        <  2:2586980/101‑1 (MQ=255)
                 aTGGGGATCGATCCGCGCACGCTTTCGGCGGGGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGGTGCCCCCC.GTCCTGCGGACTCCCCCCCGGATAttt                                                                                      >  1:4027497/1‑101 (MQ=25)
                 aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGTCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATAttt                                                                                      <  1:4325687/101‑1 (MQ=255)
                 aTGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATAttt                                                                                      <  1:1152644/101‑1 (MQ=255)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTg                                                                                     <  2:4071565/101‑1 (MQ=255)
                  tGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTg                                                                                     <  1:2999341/101‑1 (MQ=255)
                   ggggATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCCGGATATTTGa                                                                                    >  2:267588/1‑101 (MQ=255)
                       atcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCa                                                                                <  1:4225838/101‑1 (MQ=255)
                        tcgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCaa                                                                               >  1:2753652/1‑101 (MQ=255)
                         cgatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAAGCAAg                                                                              >  2:184908/1‑101 (MQ=255)
                          gatcCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGa                                                                             <  1:2572186/101‑1 (MQ=255)
                           atcCGCGCACGCTTTCGGCGGCGGCGCGCCACGCCGGAGGCCGGGCAGGGGGCCGCTGCCCCCCGGC.CTGCGGGCTCCCCCCGGGTGCTTTGAGGccggat                                                                            >  2:3380844/1‑96 (MQ=255)
                             ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                                          >  2:3959169/1‑101 (MQ=255)
                             ccGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGa                                                                          <  2:2156034/101‑1 (MQ=255)
                              cgcgCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaa                                                                         <  2:1289920/101‑1 (MQ=255)
                               gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                        <  2:1745362/101‑1 (MQ=255)
                                cgcCCGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAg                                                                       <  1:2335154/101‑1 (MQ=255)
                                  cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                     <  2:826476/101‑1 (MQ=255)
                                   ccGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                    <  1:953487/100‑1 (MQ=255)
                                     gCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGAt                                                                  <  2:3221206/101‑1 (MQ=255)
                                      cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                 <  1:142804/101‑1 (MQ=255)
                                         tCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCgg                                                              <  1:4064213/101‑1 (MQ=255)
                                            gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGcc                                                           >  1:368404/1‑100 (MQ=255)
                                             gggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                          <  1:2845949/100‑1 (MQ=255)
                                                 ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                      <  2:4477121/101‑1 (MQ=255)
                                                  gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                     <  1:755558/101‑1 (MQ=255)
                                                     ggCCACGCCTGGGGCGGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                  <  2:1073898/101‑1 (MQ=21)
                                                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                  <  2:1607290/101‑1 (MQ=255)
                                                     ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                  <  2:4950907/101‑1 (MQ=255)
                                                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                 <  1:304608/101‑1 (MQ=255)
                                                      gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                 <  2:334554/101‑1 (MQ=255)
                                                       ccACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.t                                               <  2:686553/101‑1 (MQ=255)
                                                        cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                              <  1:867968/101‑1 (MQ=255)
                                                          cGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGc                                            >  2:3410866/1‑101 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                           >  1:2418904/1‑101 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                           <  1:3203470/101‑1 (MQ=255)
                                                           gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                           <  1:3436511/101‑1 (MQ=255)
                                                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                        <  2:1086873/101‑1 (MQ=255)
                                                              tGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                        >  2:2798863/1‑101 (MQ=255)
                                                               gAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTTGGGCAAGATGAAAAACGATACCGGCGTCGATTCATCTTTGCCCCa                                        >  2:4402018/1‑101 (MQ=11)
                                                               gAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAGAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                       >  1:3501807/1‑101 (MQ=255)
                                                               gAGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                       <  1:2142989/101‑1 (MQ=255)
                                                                aGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                      <  2:3022136/101‑1 (MQ=255)
                                                                aGGCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                      <  2:50344/101‑1 (MQ=255)
                                                                 ggCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                     <  1:3858554/101‑1 (MQ=255)
                                                                  gCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                    <  1:4501029/101‑1 (MQ=255)
                                                                  gCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                    <  2:3795903/101‑1 (MQ=255)
                                                                  gCCGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                    <  2:3530945/101‑1 (MQ=255)
                                                                   ccGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAatat                                   <  2:3246675/101‑1 (MQ=255)
                                                                   ccGGGCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGAAATTTGAGGCAAGGTGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAattt                                   >  1:2452209/1‑99 (MQ=21)
                                                                     gggCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATcc                                 >  2:4204129/1‑101 (MQ=255)
                                                                     gggCAGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATACcc                                 >  2:4262044/1‑101 (MQ=255)
                                                                      ggacggggggCTCTGCCCCCC.GG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAtt                     >  2:2709821/5‑101 (MQ=2)
                                                                        acggggggCTCTGCCCCCC.GG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttgg                   >  2:4596727/3‑99 (MQ=2)
                                                                         aGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGTTCCTTAAAACCAGATAAAAGGCCATACAGGCTTCGTTTCATC.TTGCCCCCAAAATCCCggg                             >  1:4135910/1‑101 (MQ=9)
                                                                         aGGGGGGCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                             >  1:3138566/1‑101 (MQ=255)
                                                                           gggggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggggg                           >  2:3098637/1‑101 (MQ=255)
                                                                            ggggCTCTGCCCCCC.GG.CTTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttggccaa               <  1:497130/101‑7 (MQ=2)
                                                                             gggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                         <  2:1553086/101‑1 (MQ=255)
                                                                             gggCGCTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                         <  2:3485013/101‑1 (MQ=255)
                                                                             gggCGCCGCCCCCC.GTCCTGCGGGCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                         <  1:3539895/101‑1 (MQ=25)
                                                                               gcgcTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                       <  1:1771721/101‑1 (MQ=255)
                                                                               gcgcTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                       <  2:49757/101‑1 (MQ=255)
                                                                               gcgcTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                       <  1:2744571/101‑1 (MQ=255)
                                                                               cgccgccccccc.TC.CTGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAt                      <  1:175571/95‑1 (MQ=25)
                                                                                 gcTGCCCCCC.GTCCTGCGGACTCGCCACGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                     <  2:4135593/101‑1 (MQ=37)
                                                                                 gcTGCCCCCC.GTCCTGCGGACCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATA.TTGCCCCAAATATCCCGGGGGTGAAtt                     <  1:4169243/101‑1 (MQ=37)
                                                                                  cTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                    <  2:54148/101‑1 (MQ=255)
                                                                                  cTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                    <  1:1878669/101‑1 (MQ=255)
                                                                                  cTGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAttt                    >  1:4690159/1‑101 (MQ=255)
                                                                                   tGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtttt                   <  2:3911819/101‑1 (MQ=255)
                                                                                   tGCCCCCC.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtttt                   <  2:4046952/101‑1 (MQ=255)
                                                                                    gCCCCCC.GTCCTGCGGANTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTg                  <  2:274773/101‑1 (MQ=255)
                                                                                     cccccc.GTCCTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTgg                 <  1:4837390/101‑1 (MQ=255)
                                                                                           cgtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAg            <  1:3959461/97‑1 (MQ=255)
                                                                                            gtccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc           <  1:1579785/98‑1 (MQ=255)
                                                                                              ccTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCTGGGGTGAATTTTGGCGCAGCCa         <  2:123122/100‑1 (MQ=255)
                                                                                                  cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag     <  1:4286309/101‑1 (MQ=255)
                                                                                                   ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga    >  2:3294030/1‑101 (MQ=255)
                                                                                                    gACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAagag   <  2:3149488/101‑1 (MQ=255)
                                                                                                     aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg  >  2:3229945/1‑101 (MQ=255)
                                                                                                     aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg  >  1:4763358/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
CCCGGGCGCTGGGCACGATGGGGATCGATCCGCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCC.GT.CTGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGG  >  NC_014034/432333‑432532

Base quality scores:  ATCG  < 3 ≤  ATCG  < 15 ≤  ATCG  < 24 ≤  ATCG  < 33 ≤  ATCG  < 40 ≤  ATCG