Predicted mutation
evidence seq id position mutation freq annotation gene description
RA NC_014034 432,463 Δ1 bp 2.0% intergenic (+66/+82) RCAP_rcc00403 → / ← RCAP_rcc00404 hypothetical protein/Sua5/YciO/YrdC/YwlC family protein

Read alignment evidence...
  seq id position change freq score reads annotation genes product
*NC_014034432,4630A→. 2.0% 4.0 ‑2.1 ‑0.0200intergenic (+66/+82)RCAP_rcc00403/RCAP_rcc00404hypothetical protein/Sua5/YciO/YrdC/YwlC family protein
Reads supporting (aligned to +/- strand):  new base (3/1):  ref base (66/130):  total (69/131)
Fisher's exact test strand distribution p-value = 1.19e-01
Kolmogorov-Smirnov test that lower quality scores support polymorphism than reference p-value = 9.84e-01

Only 100 of 219 total aligned reads displayed.

GCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCG  >  NC_014034/432364‑432563
                                                                                                     |                                                                                                     
gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGCGGCAAGATGaaa                                                                                                       >  2:3098925/1‑101 (MQ=255)
gcgcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGaaa                                                                                                       <  2:4057177/101‑1 (MQ=255)
  gcACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGc                                                                                                     <  1:1075139/101‑1 (MQ=255)
   cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                    <  2:826476/101‑1 (MQ=255)
   cACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGcc                                                                                                    <  2:1746272/101‑1 (MQ=255)
    ccGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCg                                                                                                   <  1:953487/100‑1 (MQ=255)
       cTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATc                                                                                                <  2:537867/101‑1 (MQ=255)
        tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                               <  1:4621467/101‑1 (MQ=255)
        tttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATcc                                                                                               <  1:1566945/101‑1 (MQ=255)
         ttCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCg                                                                                              <  1:414656/101‑1 (MQ=255)
          tgggcggcgacggGTCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGGGGCAAGAT.AAACCCCATccttt                                                                                            <  2:3230350/99‑4 (MQ=14)
             gcggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGGAAGATGAAAGCCGATCCGGGcc                                                                                          >  1:368404/1‑100 (MQ=255)
              cggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCtt                                                                                         <  1:4086522/101‑1 (MQ=255)
               ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                        <  2:306514/101‑1 (MQ=255)
               ggcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTc                                                                                        <  1:1466521/101‑1 (MQ=255)
                gcggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCg                                                                                       <  2:3441428/101‑1 (MQ=255)
                  ggcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGtt                                                                                     <  2:4477121/101‑1 (MQ=255)
                   gcggGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGttt                                                                                    <  1:755558/101‑1 (MQ=255)
                    ggggCCACGCCTGAGGCGGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                   <  1:1792129/100‑1 (MQ=255)
                    cggGCCACGCCTGAGGCCGGGCAGGGGGGCNCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTc                                                                                   <  1:1612793/101‑1 (MQ=255)
                     gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                  <  2:2565931/101‑1 (MQ=255)
                     gggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCa                                                                                  <  1:1897214/101‑1 (MQ=255)
                      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                 <  1:2346572/101‑1 (MQ=255)
                      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                 <  1:2017232/101‑1 (MQ=255)
                      ggCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCAt                                                                                 <  2:4950907/101‑1 (MQ=255)
                       gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                <  2:1331622/101‑1 (MQ=255)
                       gCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATc                                                                                <  1:304608/101‑1 (MQ=255)
                         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                             <  1:867968/101‑1 (MQ=255)
                         cACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.tt                                                                             <  2:1607317/101‑1 (MQ=255)
                            gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                                                          <  1:3436511/101‑1 (MQ=255)
                            gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                                                          <  1:3203470/101‑1 (MQ=255)
                            gCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcc                                                                          >  1:2418904/1‑101 (MQ=255)
                              cTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGcccc                                                                        >  1:2894562/1‑101 (MQ=255)
                               tGAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCa                                                                       <  2:1086873/101‑1 (MQ=255)
                                gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTTGGGCAAGATGAAAAACGATACCGGCGTCGATTCATCTTTGCCCCa                                                                       >  2:4402018/1‑101 (MQ=11)
                                gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAGAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                                      >  1:3501807/1‑101 (MQ=255)
                                gAGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaa                                                                      >  2:3227401/1‑101 (MQ=255)
                                 gggCCGGGCGGGGGGGGGCTGCCCCCCTC.C.CGCCGGCCCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                    <  1:2749055/100‑1 (MQ=21)
                                 aGGCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCaaa                                                                     <  2:50344/101‑1 (MQ=255)
                                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                    <  2:140511/101‑1 (MQ=255)
                                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                    <  1:1874169/101‑1 (MQ=255)
                                  ggCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAat                                                                    <  1:3858554/101‑1 (MQ=255)
                                   gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                   <  2:2672690/101‑1 (MQ=255)
                                   gCCGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAata                                                                   <  2:3530945/101‑1 (MQ=255)
                                    ccGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAatat                                                                  <  2:3246675/101‑1 (MQ=255)
                                    ccGGGCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGAAATTTGAGGCAAGGTGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAattt                                                                  >  1:2452209/1‑99 (MQ=21)
                                      gggCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATACcc                                                                >  2:4262044/1‑101 (MQ=255)
                                        gCAGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCCTC.TTGCCCCAAATATCCCg                                                              >  1:489739/1‑101 (MQ=255)
                                         acggggggCTCTGCCCCCCGG.C.TTCG..CTCCCCCCGGGATATTTGCGGCAAGATGAA....G....GGGCATGATTTCATC.TTGCCCGAAATATCCCAGGGGTGAAttgg                                                  >  2:4596727/3‑99 (MQ=2)
                                          aGGGGGGCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCggg                                                            >  1:3138566/1‑101 (MQ=255)
                                             ggggCGCTGCCCCCCGT.CGTGCGGACACCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCAGGGGt                                                         <  2:3379116/101‑1 (MQ=25)
                                              gggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                        <  2:3485013/101‑1 (MQ=255)
                                              gggCGCTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTg                                                        <  2:1553086/101‑1 (MQ=255)
                                                gcgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                                                      <  1:1771721/101‑1 (MQ=255)
                                                gcgcTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGaa                                                      <  2:49757/101‑1 (MQ=255)
                                                  gcTGCCCCCCGTCC.TGCGGACTCGCCACGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAtt                                                    <  2:4135593/101‑1 (MQ=37)
                                                   cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                   >  1:4212722/1‑101 (MQ=255)
                                                   cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                   <  2:54148/101‑1 (MQ=255)
                                                   cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAAttt                                                   <  2:2753450/101‑1 (MQ=255)
                                                   cTGCCCCCCGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAAttt                                                   >  1:4690159/1‑101 (MQ=255)
                                                      ccccccGTCC.TGCGGACTCCCCCGGGGGTATTTGACGCTACATGAAAGCCGATCAGGGCTTCGTTTCATC.TTGCCCCAAATATACGGGGGGGGAAttttag                                                >  2:4041419/1‑99 (MQ=25)
                                                      ccccccGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTgg                                                <  1:4837390/101‑1 (MQ=255)
                                                       cccccGTCC.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCCAATCTCCCCGGGGCGAATTTTGgc                                               >  1:4017555/1‑101 (MQ=25)
                                                          ccgtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCa                                            <  1:2609886/96‑1 (MQ=255)
                                                            gtcc.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGc                                          >  2:3006000/4‑101 (MQ=255)
                                                                  gCGGACTCCCCCCGGGATATTTGAGGCAAGATGGGAGCCCATCCGGACCTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCaaaa                                     <  2:2452035/101‑1 (MQ=21)
                                                                  gCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGCATTTTGGCGCAGCCaaaa                                     >  2:4268636/1‑101 (MQ=255)
                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                    <  1:4286309/101‑1 (MQ=255)
                                                                   cGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAag                                    <  1:906611/101‑1 (MQ=255)
                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAaga                                   >  2:3294030/1‑101 (MQ=255)
                                                                    ggACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGGGAATTTTGGCGCaagatgaaac                                   >  1:4037511/1‑92 (MQ=25)
                                                                      aCTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAgg                                 >  1:4763358/1‑101 (MQ=255)
                                                                          cccccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGc                             <  2:4689836/101‑1 (MQ=255)
                                                                             ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                          <  1:1392253/101‑1 (MQ=255)
                                                                             ccGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgc                          <  2:1049039/101‑1 (MQ=255)
                                                                              cGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAgcg                         <  1:2725105/101‑1 (MQ=255)
                                                                                ggATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcc                       >  1:2912269/1‑101 (MQ=255)
                                                                                  atatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGcccc                     <  2:3181778/101‑1 (MQ=255)
                                                                                   tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                    >  2:3748885/1‑101 (MQ=255)
                                                                                   tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                    <  2:3934374/101‑1 (MQ=255)
                                                                                   tatTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGccccc                    >  2:672938/1‑101 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                   <  2:2366497/101‑1 (MQ=255)
                                                                                    atTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCt                   >  2:312182/1‑101 (MQ=255)
                                                                                     tttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTa                  >  2:3958219/1‑101 (MQ=255)
                                                                                      ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                 <  2:3828209/101‑1 (MQ=255)
                                                                                      ttGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTAt                 >  1:4457180/1‑101 (MQ=255)
                                                                                         nggNANNATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc              <  1:2332813/100‑1 (MQ=255)
                                                                                         aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc              >  1:2212981/1‑101 (MQ=255)
                                                                                         aGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATccc              >  1:1269244/1‑101 (MQ=255)
                                                                                          ggCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATcccc             >  2:1264735/1‑101 (MQ=255)
                                                                                           gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg            >  2:249247/1‑101 (MQ=255)
                                                                                           gCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcg            >  2:2031183/1‑101 (MQ=255)
                                                                                             aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg          >  1:3379658/1‑101 (MQ=255)
                                                                                             aaGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcg          >  2:2244486/1‑101 (MQ=255)
                                                                                               gATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggc        >  1:4389669/1‑101 (MQ=255)
                                                                                                 tGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgc      >  1:4986220/1‑101 (MQ=255)
                                                                                                  gAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg     <  2:4212405/101‑1 (MQ=255)
                                                                                                  gAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg     >  2:1208970/1‑101 (MQ=255)
                                                                                                  gAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCcgcggcgcg     >  2:795579/1‑101 (MQ=255)
                                                                                                     aGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGAGGCAGCGCCCCCTATCCCCgcggcgcggcg  >  1:56070/1‑101 (MQ=255)
                                                                                                     |                                                                                                     
GCGCACGCTTTCGGCGGCGGCGGGCCACGCCTGAGGCCGGGCAGGGGGGCGCTGCCCCCCGT.C.TGCGGACTCCCCCCGGGATATTTGAGGCAAGATGAAAGCCGATCCGGGCTTCGTTTCATC.TTGCCCCAAATATCCCGGGGGTGAATTTTGGCGCAGCCAAAAGAGGGGGCAGCGCCCCCTATCCCCGCGGCGCGGCG  >  NC_014034/432364‑432563

Base quality scores:  ATCG  < 3 ≤  ATCG  < 19 ≤  ATCG  < 27 ≤  ATCG  < 33 ≤  ATCG  < 41 ≤  ATCG